scholarly journals Soapwort extract supplementation alters antioxidant status of serum, liver and heart tissues in growing Japanese quails reared under chronic intermittent cold stress

2017 ◽  
Vol 86 (2) ◽  
pp. 159-165
Author(s):  
Bestami Dalkilic ◽  
Mehtap Ozcelik ◽  
Zafer Cambay ◽  
Naci Omer Alayunt ◽  
Ulku Gulcihan Simsek ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Cold Stress ◽  
Peroxidase Activity ◽  
Heart Tissue ◽  
Control Group ◽  
Glutathione Peroxidase Activity ◽  
Japanese Quails ◽  
Total Antioxidant ◽  
Oxidant Status

Antioxidant effect of dietary soapwort extract supplementation was studied in growing Japanese quails suffering from chronic intermittent cold stress. For this purpose, a total of ninety 15-d-old quails were divided into three groups with three replicates. Chronic intermittent cold stress was applied every night between 22.00 to 06.00 h; starting at 14 °C for the first week, and gradually weekly lowered to 8 °C. Three groups were fed with corn-soy based standard diets supplemented with 0, 50, and 100 ppm soapwort extract for four weeks. At the end of the study, three males and three females were slaughtered to determine total antioxidant and oxidant status of serum, malondialdehyde, glutathione, glutathione peroxidase activity, superoxide dismutase of liver and heart tissues. Although the dietary soapwort extract had no effect on serum total antioxidant capacity, it significantly lowered the total oxidant status of serum in cold stressed quails. Glutathione and superoxide dismutase enzyme activity of liver and heart tissues were similar among groups. While the dietary soapwort extract had no effect on glutathione peroxidase activity of the heart tissue, it significantly increased glutathione peroxidase activity in the liver tissue. In relation to the control group, malondialdehyde concentrations in the liver and heart tissues were significantly lower in soapwort extract groups. These data suggest that dietary soapwort extract could alleviate the detrimental effects of oxidative stress in growing Japanese quails exposed to cold stress.

Beneficial Effects of Allopurinol on Glutathione Levels and Glutathione Peroxidase Activity in Rat Ischaemic Acute Renal Failure

1996 ◽  
Vol 24 (1) ◽  
pp. 33-39 ◽  
Author(s):  
Ö Alataş ◽  
A Şahin ◽  
Ö Çolak ◽  
M Inal ◽  
T Köken ◽  
...  
Keyword(s):  
Placebo Group ◽  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Control Group ◽  
Left Renal Artery ◽  
Glutathione Peroxidase Activity ◽  
Beneficial Effects ◽  
Renal Ischaemia ◽  
Ischaemia Reperfusion ◽  
Glutamyl Transferase

The protective effect of allopurinol, an inhibitor of the enzyme, xanthine oxidase, against the renal ischaemia–reperfusion of the rat was investigated. Rats were subjected to renal ischaemia by clamping of the left renal artery and vein for 45 min, and were then reperfused for 24 h; these animals were randomized to receive either saline ( n = 10) or allopurinol ( n = 10) at a dose of 50 mg/kg bolus intraperitoneally 5 min before reperfusion. The control group comprised seven healthy rats not exposed to ischaemia or reperfusion. The blood urea nitrogen and plasma creatinine levels were increased in the allopurinol group, but the increase was less than that in the placebo group, compared with the controls. The kidney glutathione level was significantly reduced in the placebo group but not in the allopurinol group compared with the controls. The glutathione peroxidase activity in the kidney tissues was reduced more than two-fold in the placebo group compared with the controls, but the reduction in glutathione peroxidase was considerably less in the allopurinol group. Renal tissue lactate dehydrogenase, aspartate amino transferase, γ-glutamyl transferase and alkaline phosphatase activities were reduced almost two-fold in the placebo group, but allopurinol treatment maintained these enzyme activities close to the control activities. These results provide evidence that allopurinol treatment may have beneficial effects on antioxidant defences against ischaemia-reperfusion injury of rat kidneys.

scholarly journals Effects of estrogens and androgens on erythrocyte antioxidant superoxide dismutase, catalase and glutathione peroxidase activities during the menstrual cycle

2000 ◽  
Vol 167 (3) ◽  
pp. 447-452 ◽  
Author(s):  
C Massafra ◽  
D Gioia ◽  
C De Felice ◽  
E Picciolini ◽  
V De Leo ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Menstrual Cycle ◽  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Free Testosterone ◽  
Cycle Phase ◽  
Activity Levels ◽  
Glutathione Peroxidase Activity ◽  
Antioxidant Enzyme System ◽  
Peroxidase Enzyme

The effects of physiological changes in estrogens and androgens on the erythrocyte antioxidant superoxide dismutase, catalase and glutathione peroxidase enzyme activities during the menstrual cycle were investigated in healthy eumenorrheic women. Blood samples were taken on alternate days from twelve normally cyclic women (age range: 20 to 27 years; mean age: 24.1 years) from the first day of one menstrual cycle until the first day of the subsequent one. Plasma was analyzed for FSH, LH, estradiol, progesterone, testosterone, free testosterone and androstenedione concentrations. Erythrocyte superoxide dismutase, catalase and glutathione peroxidase activities were evaluated on the same days and cycle length was standardized on the basis of the preovulatory estradiol peak. Significant cyclic phase-related changes were observed in glutathione peroxidase (P<0.05), with higher glutathione peroxidase activity levels from the late follicular to the early luteal phase compared with those found in the early follicular phase (P<0.001 and P<0.002 respectively). A significant positive correlation was observed between mean estradiol and glutathione peroxidase cycle-related variations (r=0.80, P<0.001), whereas no significant cycle phase-dependent changes were seen in superoxide dismutase and catalase. No effect of progesterone and androgens on the erythrocyte antioxidant enzyme system was documented. The findings indicate that physiological ovarian estradiol production during the menstrual cycle may have an important role in regulating erythrocyte glutathione peroxidase activity.

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