scholarly journals West Nile virus outbreak in humans and epidemiological surveillance, west Andalusia, Spain, 2016

2018 ◽  
Vol 23 (14) ◽  
Author(s):  
Nuria López-Ruiz ◽  
María del Carmen Montaño-Remacha ◽  
Enric Durán-Pla ◽  
Mercedes Pérez-Ruiz ◽  
Jose María Navarro-Marí ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Wild Birds ◽  
Epidemiological Surveillance ◽  
Southern Europe ◽  
West Nile ◽  
Transmission Season ◽  
Epidemiological Changes ◽  
First Time ◽  
Regional Surveillance

In Andalusia, Spain, West Nile virus (WNV) surveillance takes place from April to November, during the active vector period. Within this area seroconversion to this virus was evidenced in wild birds in 2004, affecting horses and two humans for the first time in 2010. Since 2010, the virus has been isolated every year in horses, and national and regional surveillance plans have been updated with the epidemiological changes found. WNV is spreading rapidly throughout southern Europe and has caused outbreaks in humans. Here we describe the second WNV outbreak in humans in Andalusia, with three confirmed cases, which occurred between August and September 2016, and the measures carried out to control it. Surveillance during the transmission season is essential to monitor and ensure prompt identification of any outbreaks.

A Snapshot Avian Surveillance Reveals West Nile Virus and Evidence of Wild Birds Participating in Toscana Virus Circulation

2017 ◽  
Vol 17 (10) ◽  
pp. 698-708 ◽  
Author(s):  
Sabri Hacioglu ◽  
Ender Dincer ◽  
Cafer Tayer Isler ◽  
Zeynep Karapinar ◽  
Veysel Soydal Ataseven ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Wild Birds ◽  
Toscana Virus ◽  
West Nile

The survey of wild birds for West Nile virus in Poland

2011 ◽  
Vol 14 (4) ◽  
pp. 573-577 ◽  
Author(s):  
J. Niczyporuk ◽  
E. Samorek-Salamonowicz ◽  
W. Kozdruń ◽  
Z. Mizak
Keyword(s):  
West Nile Virus ◽  
Genetic Material ◽  
Early Spring ◽  
Wild Birds ◽  
Rt Pcr ◽  
West Nile ◽  
Late Autumn ◽  
The Brain

The survey of wild birds for West Nile virus in PolandTwo thousand one hundred and forty birds belonging to 39 different species from different locations in Poland were examined. The study has taken place from the early spring till late autumn 2007-2010 when the activity of the mosquitoes was the highest. The brain samples were taken from the birds and whole cellular RNA was isolated, then the RT-PCR and NRT-PCR were performed to detect the presence of West Nile virus (WNV). The obtained results were confirmed by the commercial WNV Kit. No genetic material of WNV was found in the examined samples.

Surveillance on antibodies against West Nile virus, Usutu virus, tick-borne encephalitis virus and Tribeč virus in wild birds in Drienovská wetland, Slovakia

Biologia ◽  
2019 ◽  
Vol 74 (7) ◽  
pp. 813-820 ◽  
Author(s):  
Tomáš Csank ◽  
Ľuboš Korytár ◽  
Terézia Pošiváková ◽  
Tamás Bakonyi ◽  
Juraj Pistl ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Wild Birds ◽  
Encephalitis Virus ◽  
West Nile ◽  
Usutu Virus ◽  
Tick Borne Encephalitis ◽  
Tick Borne Encephalitis Virus

scholarly journals Genome sequence analysis of the first human West Nile virus isolated in Italy in 2009

2009 ◽  
Vol 14 (44) ◽  
Author(s):  
L Barzon ◽  
E Franchin ◽  
L Squarzon ◽  
E Lavezzo ◽  
S Toppo ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Genome Sequence ◽  
Virus Transmission ◽  
Whole Genome Sequence ◽  
Veneto Region ◽  
West Nile ◽  
Phylogenetic Relatedness ◽  
Neuroinvasive Disease ◽  
Human West Nile Virus ◽  
First Time

In 2009, six new human cases of West Nile neuroinvasive disease (WNND) were identified in Veneto region, following the six cases already reported in 2008. A human West Nile virus (WNV) isolate was obtained for the first time from an asymptomatic blood donor. Whole genome sequence of the human WNV isolate showed close phylogenetic relatedness to the Italy-1998-WNV strain and to other WNV strains recently isolated in Europe, with the new acquisition of the NS3-Thr249Pro mutation, a trait associated with avian virulence, increased virus transmission, and the occurrence of outbreaks in humans.

West Nile virus lineage 2 in Sardinian wild birds in 2012: a further threat to public health

2013 ◽  
Vol 141 (11) ◽  
pp. 2313-2316 ◽  
Author(s):  
G. SAVINI ◽  
G. PUGGIONI ◽  
A. DI GENNARO ◽  
G. DI FRANCESCO ◽  
A. M. ROCCHIGIANI ◽  
...  
Keyword(s):  
Public Health ◽  
West Nile Virus ◽  
Sequence Analysis ◽  
Wild Birds ◽  
Homologous Sequence ◽  
West Nile ◽  
Lineage 2 ◽  
High Level

SUMMARYWest Nile virus (WNV) strains belonging to lineage 2 were detected and isolated from the tissues of a goshawk and two carrion crows in Sardinia in August 2012. According to NS3 sequence analysis, the Sardinian isolates shared a high level of similarity with those of Italian lineage 2 strains which circulated in 2011 and with the homologous sequence of the 2004 Hungarian isolate. Following the human fatality reported in 2011 in Olbia, this study is the first to report the spread and enzootic circulation of WNV lineage 2 in Sardinia.

scholarly journals Co-Circulation of West Nile and Usutu Flaviviruses in Mosquitoes in Slovakia, 2018

Viruses ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 639 ◽  
Author(s):  
Viktória Čabanová ◽  
Silvie Šikutová ◽  
Petra Straková ◽  
Oldřich Šebesta ◽  
Bronislava Vichová ◽  
...  
Keyword(s):  
European Union ◽  
West Nile Virus ◽  
The European Union ◽  
West Nile ◽  
Usutu Virus ◽  
Pathogenic Viruses ◽  
Lineage 2 ◽  
First Time

Monitoring West Nile virus (WNV) and Usutu virus (USUV) activity now has the highest priority among mosquito-borne pathogenic viruses circulating in the European Union. This study documents a first time detection and the co-circulation of WNV lineage-2 (with the minimal prevalence of 0.46%) and USUV clade Europe 2 (with the minimal prevalence of 0.25%) in mosquitoes from the same habitat of south-western Slovakia and underlines necessity to perform rigorous surveillance in birds, mosquitoes, horses and humans in that country.

scholarly journals West Nile Virus Mosquito Vectors (Diptera: Culicidae) in Germany

Viruses ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 493 ◽  
Author(s):  
Helge Kampen ◽  
Cora M. Holicki ◽  
Ute Ziegler ◽  
Martin H. Groschup ◽  
Birke Andrea Tews ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Virus Strain ◽  
Envelope Protein ◽  
Protein Gene ◽  
Viral Envelope ◽  
Mosquito Vectors ◽  
Viral Envelope Protein ◽  
West Nile ◽  
Qrt Pcr ◽  
First Time

In 2018, West Nile virus (WNV) broke out for the first time in Germany, with continuation of the epidemic in 2019, involving birds, horses and humans. To identify vectors and characterize the virus, mosquitoes were collected in both years in zoological gardens and on a horse meadow immediately following the diagnosis of disease cases in birds and horses. Mosquitoes were identified and screened for WNV by qRT-PCR, with virus-positive samples being sequenced for the viral envelope protein gene. While no positive mosquitoes were found in 2018, seven mosquito pools tested positive for WNV in 2019 in the Tierpark (Wildlife Park) Berlin. The pools consisted of Cx. pipiens biotype pipiens (n = 5), and a mixture of Cx. p. biotype pipiens and Cx. p. biotype molestus (n = 2), or hybrids of these, and were collected between 13 August and 24 September 2019. The virus strain turned out to be nearly identical to two WNV strains isolated from birds diseased in 2018 in eastern Germany. The findings represent the first demonstration of WNV in mosquitoes in Germany and include the possibility of local overwintering of the virus.

Attempts to detect West Nile virus in wild birds in Poland

2011 ◽  
Vol 59 (3) ◽  
pp. 405-408 ◽  
Author(s):  
Jowita Niczyporuk ◽  
Elżbieta Samorek-Salamonowicz ◽  
Wojciech Kozdruń ◽  
Zbigniew Mizak
Keyword(s):  
West Nile Virus ◽  
Early Spring ◽  
Wild Birds ◽  
Rt Pcr ◽  
West Nile ◽  
Late Autumn ◽  
The Brain

The aim of the study was to attempt the detection of West Nile virus (WNV) in wild birds in Poland. Forty-eight species of 1912 wild birds were used for the investigations. The birds were derived from various locations in Poland from early spring till late autumn of the years 2009–2011. The brain samples were homogenised and cellular RNA was isolated. Two methods (RT-PCR and nested RT-PCR) were used. The presence of WNV RNA was not detected in the samples examined. Additionally, a short analysis of the epizootiological situation regarding the presence of WNV in Poland is presented.

scholarly journals West Nile virus strain Kunjin NS5 polymerase is a phosphoprotein localized at the cytoplasmic site of viral RNA synthesis

2007 ◽  
Vol 88 (4) ◽  
pp. 1163-1168 ◽  
Author(s):  
Jason M. Mackenzie ◽  
Mark T. Kenney ◽  
Edwin G. Westaway
Keyword(s):  
West Nile Virus ◽  
Rna Polymerase ◽  
Virus Strain ◽  
Viral Rna ◽  
West Nile ◽  
Replication Complex ◽  
Virus Rna ◽  
West Nile Virus Strain ◽  
First Time ◽  
Cytoplasmic Location

Using West Nile virus strain Kunjin virus (WNVKUN) as a model system for flavivirus replication, we showed that the virus replication complex (RC) is associated with the dsRNA template located in induced membranes only in the cytoplasm. In this report we established for the first time that the RNA-dependent RNA polymerase NS5 is located in flavivirus-induced membranes, including the site of viral RNA replication. We found no evidence for nuclear localization of the essential RC components NS5 and its dsRNA template for WNVKUN or the closely related WNV strain Sarafend, by immuno-electron microscopy or by immunofluorescence. Metabolic radiolabelling with [32P]orthophosphate revealed that WNVKUN NS5 was phosphorylated and this was confirmed by Western blotting with antibodies specific for phosphorylated serine and threonine only. These observations of a cytoplasmic location for the WNV polymerase and its phosphorylation state correspond to the characteristics of the hepatitis C virus RNA polymerase NS5B.

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