scholarly journals Cases of Salmonella Urbana in Finland, the Czech Republic and Latvia, January-February 2010

2010 ◽  
Vol 15 (11) ◽  
Author(s):  
R Rimhanen-Finne ◽  
S Lukinmaa ◽  
T Martelius ◽  
H Rossow ◽  
R Karpíšková ◽  
...  
Keyword(s):  
Czech Republic ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Common Source ◽  
The Czech Republic ◽  
Pulsed Field ◽  
Source Of Infection ◽  
Pfge Profiles

A cluster of 14 cases of Salmonella Urbana cases in Finland, the Czech Republic and Latvia were identified in January-February, 2010. The majority of cases (11) were male and children under 16 years of age. The investigation is currently ongoing and comparison of pulsed-field gel electrophoresis (PFGE) profiles of the isolates suggests that the cases may have a common source of infection.

scholarly journals Typing of Human Campylobacter jejuniIsolates in Finland by Pulsed-Field Gel Electrophoresis

1998 ◽  
Vol 36 (6) ◽  
pp. 1787-1789 ◽  
Author(s):  
Marja-Liisa Hänninen ◽  
Sini Pajarre ◽  
Marja-Liisa Klossner ◽  
Hilpi Rautelin
Keyword(s):  
Campylobacter Jejuni ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Common Source ◽  
Pulsed Field ◽  
Source Of Infection

A total of 69 pulsed-field gel electrophoresis (PFGE) types were identified among 176 Campylobacter jejuni isolates from Finnish patients. In two geographic areas studied, five predominant PFGE types comprised over 40% of the isolates. One-third of the isolates had unique PFGE types. In small outbreaks, identical PFGE patterns were demonstrated, indicating a common source of infection.

Isolation and Pulsed-Field Gel Electrophoresis Typing of Listeria monocytogenes from Modified Atmosphere Packaged Fresh-Cut Vegetables Collected in Ireland

2006 ◽  
Vol 69 (10) ◽  
pp. 2524-2528 ◽  
Author(s):  
GILLIAN A. FRANCIS ◽  
DAVID O'BEIRNE
Keyword(s):  
Listeria Monocytogenes ◽  
Gel Electrophoresis ◽  
Fruits And Vegetables ◽  
Pulsed Field Gel Electrophoresis ◽  
Leafy Vegetables ◽  
Modified Atmosphere ◽  
Pulsed Field ◽  
Fruit Samples ◽  
Fresh Cut ◽  
Pfge Profiles

The incidence of Listeria monocytogenes in modified atmosphere packaged fresh-cut fruits and vegetables from chill cabinets of a supermarket in Ireland was investigated over a 2-year period. Overall, 9.58% of fresh-cut produce was contaminated with Listeria spp. Various species of Listeria were isolated from samples, including L. monocytogenes, L. seeligeri, L. innocua, L. welshimeri, and L. ivanovii. No fruit samples contained detectable L. monocytogenes. Overall, a total of 21 L. monocytogenes isolates (2.9% of samples) were recovered from a range of products, including dry coleslaw mix (80% shredded cabbage and 20% shredded carrot), bean sprouts, and leafy vegetables such iceberg, romaine, and radicchio lettuce and mixed salad leaves (curly endive, escarole, and radicchio leaves). Dry coleslaw mix appeared to have the highest incidence of Listeria contamination (20%) compared with other products. Listeria contamination was more frequent (P < 0.05) during the summer and autumn months than during the winter and spring months. The 21 L. monocytogenes isolates were subsequently subtyped by genomic macrorestriction techniques using ApaI with pulsed-field gel electrophoresis (PFGE). PFGE of digested DNA produced bands of 79 to 518 kb. Four PFGE profiles were identified, and approximately 50% of the isolates were associated with profile 1. This study indicates that fresh-cut vegetables packaged under a modified atmosphere can support growth of numerous species of Listeria, including L. monocytogenes.

scholarly journals Molecular typing of Escherichia coli O157[ratio ]H7 (H−) isolates from cattle in Japan

1999 ◽  
Vol 122 (2) ◽  
pp. 337-341 ◽  
Author(s):  
M. AKIBA ◽  
T. MASUDA ◽  
T. SAMESHIMA ◽  
K. KATSUDA ◽  
M. NAKAZAWA
Keyword(s):  
Escherichia Coli ◽  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Escherichia Coli O157 ◽  
Outbreak Strain ◽  
Pulsed Field ◽  
E Coli ◽  
Biological Methods ◽  
Pfge Profiles

A total of 77 Escherichia coli O157[ratio ]H7 (H−) isolates from cattle in Japan were investigated by molecular biological methods. Most of these isolates (43 isolates) possessed the stx2 gene, but not stx1. Fifteen bacteriophage types and 50 pulsed-field gel electrophoresis (PFGE) profiles were observed. One isolate was indistinguishable from the human outbreak strain by these methods. This indicates that cattle must be considered as a possible source of human E. coli O157[ratio ]H7 infection in Japan.

Outbreak ofSerratia marcescensInfection in a Neonatal Intensive Care Unit

2001 ◽  
Vol 22 (5) ◽  
pp. 303-305 ◽  
Author(s):  
G.A. Prasad ◽  
Paula G. Jones ◽  
Jacqueline Michaels ◽  
Jeffery S. Garland ◽  
Chandra R. Shivpuri
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Neonatal Intensive Care Unit ◽  
Serratia Marcescens ◽  
Gel Electrophoresis ◽  
Neonatal Intensive Care ◽  
Community Hospital ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Source Of Infection

AbstractWe report an outbreak ofSerratia marcescensinfection in the neonatal intensive care unit of a community hospital. The outbreak involved eight neonates, (five infected and three colonized), one of whom died. Pulsed-field gel electrophoresis confirmed that all isolates were identical strains. Cohorting and isolation of the infected neonates helped to control the outbreak. No environmental source of infection was found.

scholarly journals Intermediately virulent Rhodococcus equi isolates from pigs in Slovenia: discovery of new plasmid types and assessment of genetic diversity by pulsed-field gel electrophoresis

2009 ◽  
Vol 54 (No. 3) ◽  
pp. 111-117 ◽  
Author(s):  
M. Pate ◽  
M. Ocepek ◽  
I. Zdovc ◽  
C. Minato ◽  
Y. Ohtsu ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Gel Electrophoresis ◽  
Detailed Comparison ◽  
Rhodococcus Equi ◽  
Pulsed Field Gel Electrophoresis ◽  
High Genetic Diversity ◽  
Pulsed Field ◽  
Large Plasmids ◽  
Multiple Strains ◽  
Pfge Profiles

The presence of large plasmids in 30 <I>Rhodococcus equi</I> strains isolated from pig lymph nodes with granulomatous changes was investigated. Plasmid DNAs were isolated and digested with the restriction endonucleases <I>Bam</I>HI, <I>Eco</I>RI, <I>Eco</I>T22I and <I>Hind</I>III for detailed comparison and estimation of plasmid sizes. A total of nine isolates were identified as intermediately virulent (VapB-positive), harbouring large plasmids of type 5 (<I>n</I> = 5) and four new variants that we tentatively designated as type 19 (<I>n</I> = 1), 20 (<I>n</I> = 1), 21 (<I>n</I> = 1) and 24 (<I>n</I> = 1). All isolates were subjected to genotyping with pulsed-field gel electrophoresis (PFGE). High genetic diversity was observed: 21 distinct genotypes were detected; five were found in multiple isolates and the others were unique. Isolates of the same plasmid type exhibited different PFGE profiles and vice versa. In a few cases, multiple strains from certain farms were analysed, the majority of which exhibited diverse PFGE profiles. Our findings demonstrate the presence of a wide variety of <I>R. equi</I> strains even in small confined environments such as farms. This is the first molecular epidemiology study of intermediately virulent <I>R. equi</I> isolates from Slovenian pigs.

Comparison of Danish Isolates of Salmonella entericaSerovar Enteritidis PT9a and PT11 from Hedgehogs (Erinaceus europaeus) and Humans by Plasmid Profiling and Pulsed-Field Gel Electrophoresis

2000 ◽  
Vol 38 (10) ◽  
pp. 3631-3635 ◽  
Author(s):  
B. Nauerby ◽  
K. Pedersen ◽  
H. H. Dietz ◽  
M. Madsen
Keyword(s):  
Gel Electrophoresis ◽  
Fragment Length Polymorphism ◽  
Phage Type ◽  
Pulsed Field Gel Electrophoresis ◽  
Clonal Lineage ◽  
S1 Nuclease ◽  
Pulsed Field ◽  
Plasmid Profiling ◽  
Susceptibility Patterns ◽  
Pfge Profiles

During the years 1994 to 1998, 10 strains of Salmonella enterica serovar Enteritidis phage type 11 (PT11) and 6 PT9a strains were isolated from Danish hedgehogs, together with 7 strains that did not yield phage susceptibility patterns conforming with any known phage type (routine dilution no conformity [RDNC]). From 1995 to 1998, five Danish patients were reported infected with serovar Enteritidis PT11 and two with PT9a. All serovar Enteritidis PT11, PT9a, and RDNC isolates from hedgehogs and humans were analyzed by pulsed-field gel electrophoresis (PFGE), plasmid profiling, and restriction fragment length polymorphism (RFLP) of plasmids. By use of S1 nuclease and HindIII, the PT11 and PT9a isolates had identical plasmid profiles and RFLP patterns, which differed from the RDNC profiles. The PFGE profiles were identical for all serovar Enteritidis PT11 and PT9a strains from hedgehogs, four of five human strains of serovar Enteritidis PT11, and two human strains of serovar Enteritidis PT9a, irrespective of restriction enzyme, whereas the last human strain deviated slightly when NotI was used but not when XbaI or SpeI was used. The results indicate that serovar Enteritidis PT9a and PT11 are closely related and that PT11 and PT9a from Danish hedgehogs and humans belong to the same clonal lineage.

scholarly journals Pulsed-Field Gel Electrophoresis Profile Changes Resulting from Spontaneous Chromosomal Deletions in Enterohemorrhagic Escherichia coli O157:H7 during Passage in Cattle

2009 ◽  
Vol 75 (17) ◽  
pp. 5719-5726 ◽  
Author(s):  
Noriyo Yoshii ◽  
Yoshitoshi Ogura ◽  
Tetsuya Hayashi ◽  
Takashi Ajiro ◽  
Toshiya Sameshima ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Enterohemorrhagic Escherichia Coli ◽  
Sequencing Analysis ◽  
Pulsed Field ◽  
Chromosomal Deletions ◽  
Profile Changes ◽  
Dna Sequencing Analysis ◽  
Pfge Profiles

ABSTRACT A total of 905 enterohemorrhagic Escherichia coli (EHEC) O157:H7 isolates that were recovered from experimentally infected cattle, in addition to the inoculated strain, were analyzed by pulsed-field gel electrophoresis (PFGE). Twelve PFGE profiles other than that of the inoculated strain were observed. We successfully identified five distinct chromosomal deletions that affected the PFGE profiles using whole-genome PCR scanning and DNA sequencing analysis. The changes in PFGE profiles of EHEC O157:H7 isolates after passage through the intestinal tract of cattle were partially generated by deletion of chromosomal regions.

scholarly journals Whole-Genome Sequences of Two Listeria monocytogenes Serovar 1/2a Strains Responsible for a Severe Listeriosis Outbreak in Central Italy

2018 ◽  
Vol 6 (24) ◽  
Author(s):  
Massimiliano Orsini ◽  
Alessandra Cornacchia ◽  
Claudio Patavino ◽  
Marina Torresi ◽  
Patrizia Centorame ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Gel Electrophoresis ◽  
Central Italy ◽  
Pulsed Field Gel Electrophoresis ◽  
Single Band ◽  
Whole Genome ◽  
Genome Sequences ◽  
Content Type ◽  
Pulsed Field ◽  
Pfge Profiles

ABSTRACT We report the whole-genome sequences of two Listeria monocytogenes strains responsible for a severe invasive listeriosis outbreak in central Italy that occurred in 2015 and 2016. These two strains differ by a single band in their pulsed-field gel electrophoresis (PFGE) profiles.

scholarly journals Epidemiologic application of pulsed-field gel electrophoresis to an outbreak of Campylobacter jejuni in an Austrian youth centre

2000 ◽  
Vol 125 (1) ◽  
pp. 13-16 ◽  
Author(s):  
A. LEHNER ◽  
C. SCHNECK ◽  
G. FEIERL ◽  
P. PLESS ◽  
A. DEUTZ ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Restriction Endonucleases ◽  
Clinical Samples ◽  
Pulsed Field ◽  
Source Of Infection ◽  
Unpasteurized Milk ◽  
Stool Samples ◽  
Youth Centre

We report the first documented Campylobacter jejuni outbreak in an Austrian youth centre. Sixty-four children were involved of which 38 showed classical signs of campylobacter gastroenteritis. Since unpasteurized milk distributed by a local dairy was suspected to be the source of infection, stool samples were collected from 20 cows providing the milk. Five of the cows tested positive for C. jejuni. These isolates together with 37 clinical samples were compared by pulsed-field-gel electrophoresis (PFGE). The PFGE patterns, using the restriction endonucleases SmaI and SalI, were identical for the human and bovine isolates.This finding confirmed that the outbreak was caused by the consumption of unpasteurized milk contaminated with C. jejuni.

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