scholarly journals Effect of Autologous Platelet-Rich Plasma (PRP) Treatment on Endometrial Receptivity in ICSI cases

2021 ◽  
Vol 10 (2) ◽  
pp. 73-89
Author(s):  
Wafaa Rahmatullah ◽  
Manal Al-Obaidi ◽  
Huda Hussaini
Keyword(s):  
Growth Factors ◽  
Endometrial Thickness ◽  
Platelet Rich Plasma ◽  
Embryo Implantation ◽  
Power Doppler ◽  
Resistance Index ◽  
Serum Levels ◽  
Endometrial Receptivity ◽  
Ultrasound Findings ◽  
Autologous Platelet Rich Plasma

The crosstalk between a receptive endometrium and a functional blastocyst during human embryo implantation is crucial for conception. Because platelets rich plasma (PRP) with concentrated platelets 4-5 times higher than normal, when release of granules containing growth factors including VEGF, TGF, PDGF, IGF, and EGF, these factors involved for sub endometrial angiogenesis and endometrial receptivity. 44 women under the age of 40 were given antagonist ovarian stimulation treatments, the oocytes were harvested utilizing 2-D power doppler ultrasound guidance, then ICSI done for them. On the hCG day all had an intrauterine autologous PRP infusion. Power Doppler is utilized to measure endometrial thickness (EnT), pulsatility index (PI), and resistance index (RI) of sub endometrial arteries, as well as serum levels of VEGF and EGF were measured on the hCG day and ovum pickup (OPU). The ultrasound findings, on the hCG day compared to those of OPU in all women with intrauterine infused autologous PRP were highly significant (HS). The mean EnT, RI, PI, VEGF and EGF at OPU day was lower than that on hCG day in a HS manner, with (p < 0.001), (p < 0.001), (p = 0.047), (p < 0.001), and (p < 0.001) respectively. These characteristics were shown to be significantly and independently associated to intrauterine PRP infusion. After PRP injection, both growth factors serum levels (VEGF and EGF) increased, and ultrasonography sub-endometrial metrics such as EnT, RI, and PI changed as the thickness grew while vascular resistance decreased, and all considered as predictors of endometrial receptivity.

scholarly journals ROLE OF PLATELET RICH PLASMA IN TREATMENT OF CHRONIC NON-HEALING VENOUS ULCER

2020 ◽  
Vol 4 (2) ◽  
Author(s):  
Firdaus A. Dekhaiya ◽  
Jignesh K. Joshi ◽  
Sarav Bamania
Keyword(s):  
Growth Factors ◽  
Platelet Rich Plasma ◽  
Autologous Blood ◽  
Healing Process ◽  
Venous Ulcer ◽  
Lower Limbs ◽  
Venous Ulcers ◽  
Autologous Platelet Rich Plasma ◽  
Adhesive Proteins ◽  
Autologous Platelet

Introduction: Venous ulcers are wounds that are thought to occur due to improper functioning of venous valves, usually of the lower limbs. Considering that PRP is a source of growth factors, and consequently has mitogenic, angiogenic, and chemotactic properties, it represents an adjunctive treatment for recalcitrant wounds. Moreover, PRP provides the wound with adhesive proteins, such as fibrinogen, which are important in wound healing. PRP contain more amount of platelets, cytokines and growth factors which are dispersed in a very small amount of plasma which can be prepared from a sample of centrifuged autologous blood. Application of PRP has been reported to be effective in both acute as well as chronic non healing venous ulcers. Aim and Objective: To evaluate the efficacy of autologous platelet rich plasma in the management of chronic venous ulcer. Material and Method: A Prospective study conducted on 100 patients of chronic venous ulcers admitted in Sir T. Hospital Bhavnagar from June 2018 to June 2019 after fulfilled our inclusion and exclusion criteria. PRP then injected intalesionally inside and around the periphery of the wound/ulcer. This process was done once/week for 12 weeks. At every week, the area and volume of ulcer was calculated and photographs were taken. Result: All the patients showed healing of the ulcer with reduction in size of ulcer more than 90% was observed in 72 patients, followed by 80–90% reduction in wound size in 18 patients after the 12 weeks follow-up. Overall, significant reduction in size of ulcer was observed in all the treated patients. Conclusion: Autologous platelet rich plasma (PRP) as an autologous method, it is biocompatible, simple, safe, affordable and less expensive procedure in the treatment of chronic venous ulcers. PRP is found to be useful in improving and enhancing the healing process in chronic venous leg ulcers without any side effect. Keywords:  Venous ulcer, Platelet rich plasma

scholarly journals Intrauterine Infusion of Autologous Platelet-Rich Plasma Affects the Endometrial Thickness, Epidermal Growth Factor and Pregnancy Outcome in Patients Undergoing IUI

2020 ◽  
Vol 9 (2) ◽  
Author(s):  
Wafaa A. Abaid ◽  
◽  
Manal T. Al-Obaidi ◽  
Muayad S. Abood ◽  
◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Endometrial Thickness ◽  
Platelet Rich Plasma ◽  
Chorionic Gonadotrophin ◽  
Human Chorionic Gonadotrophin ◽  
Epidermal Growth ◽  
Autologous Platelet Rich Plasma ◽  
The Mean ◽  
Autologous Platelet

Despite developments in assisted reproductive technology, there is immaterial progress in the implantation and pregnancy rates. Intrauterine infusion (IUIF) of autologous platelet-rich plasma (PRP) might renew implantation rates through its paracrine properties by progression cytokines and growth factors which favor implantation. Here we determine whether the IUIF of autologous PRP had a role in pregnancy outcome through its outcome on epidermal growth factor and endometrial thickness. An overall of 43 patients where prospectively randomly dispersed into two groups subjected to a superovulation program using Letrozole® tablet orally 2.5 mg twice daily 12 hours apart from day 2 for 5 days for one cycle. 20 women were considered as control receiving the conventional intrauterine insemination (IUI) management while 23 of them were given PRP by IUIF on the day of human chorionic gonadotrophin injection. The IUI was done for both groups 36-48 hours after confirming ovulation. The blood samples were collected from both groups on the day of IUI for the valuation of epidermal growth factor and an ultrasound was done on the day of human chorionic gonadotrophin injection and day of IUI for assessment of endometrial thickness. The mean endometrial thickness in the PRP group at the day of IUI was significantly thicker than that of the control group and the difference in percentage change of endometrial thickness between PRP group and controls significantly higher in PRP group. The mean epidermal growth factor and the pregnancy rate were significantly superior in the PRP group than that of controls. In conclusion, autologous PRP IUIF was well-tolerated and resulted in a significant expansion in endometrial thickness, epidermal growth factor Level and, subsequent pregnancy rate in an infertile woman undergoing IUI.

scholarly journals Treatment of unexplained thin endometrium with autologous platelet-rich plasma in a frozen embryo transfer cycle

2019 ◽  
Vol 2 (2) ◽  
pp. 80-82
Author(s):  
Sara Mahmood ◽  
Salma Kafeel ◽  
Riffat Bibi ◽  
Naveed Iqbal
Keyword(s):  
Embryo Transfer ◽  
Endometrial Thickness ◽  
Platelet Rich Plasma ◽  
Uterine Cavity ◽  
Blastocyst Stage ◽  
Frozen Embryo Transfer ◽  
Thin Endometrium ◽  
Autologous Platelet Rich Plasma ◽  
Autologous Platelet ◽  
Endometrial Proliferation

Inadequate endometrial proliferation is a known cause of implantation failure in assisted reproductive technology cycles. It is generally agreed that >9 mm endometrial thickness is associated with higher implantation. Several strategies have been explored to enhance endometrial proliferation. However, the results are either poor, inconsistent or subject to safety concerns. A 34-year old woman presented with unexplained thin endometrium in successive frozen embryo transfer (FET) cycles. Autologous platelet-rich plasma (PRP) was infused in the uterine cavity on day 10 of second FET cycle enhancing endometrial thickness, which post-PRP infusion measured 10.9 mm. Blastocyst-stage embryos were transferred resulting in a successful pregnancy. Autologous intrauterine infusion of PRP positively impacts endometrial proliferation and implantation which is safe, low resource and minimally invasive.

scholarly journals REPARATIVE PROCESSES FEATURES IN TROPHIC ULCERS CAUSED BY DIABETES MELLITUS WITH THE USE OF PLATELET-RICH PLASMA

2020 ◽  
Vol 73 (10) ◽  
pp. 2150-2155
Author(s):  
Igor D. Duzhiy ◽  
Andrii S. Nikolaienko ◽  
Vasyl M. Popadynets ◽  
Oleksandr V. Kravets ◽  
Igor Y. Hresko ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Growth Factors ◽  
Comparison Group ◽  
Platelet Rich Plasma ◽  
Main Group ◽  
Lower Limbs ◽  
Before And After ◽  
Autologous Platelet Rich Plasma ◽  
Inflammatory Changes ◽  
Autologous Platelet

The aim: Was following: the improvement of the treatment results of the lower limbs ulcers, caused by the diabetes mellitus by using our technique of the platelet-rich plasma application; the study of the features of the morphological and immunohistochemical changes, and the effect of the growth factors of the platelet-rich plasma on the regeneration and healing of the ulcers. Materials and methods: 38 patients with the trophic ulcers of the lower limbs, caused by diabetes mellitus were involved in the study. To assess the morphological features of the reparative processes before and after the treatment with PRP, the histological and immunohystochemical studies of the biopsy specimen of ulcers were carried out. Results: The total epithelialization of the ulcers in the patients from the main group was achieved on 46.5 day, and in the comparison group – 81.7 day. The light and optical study of the specimen of the main group, using the autologous platelet-rich plasma revealed the elimination of the destructive changes and reduction of the mixed-cellular infiltration compared to the patients from the comparison group. The immunohystochemical study revealed the increase of the growth factors receptors. Conclusions: The usage of the autologous platelet-rich plasma in the treatment of the trophic ulcers results in the rapid regeneration by the decrease of the destructive and inflammatory changes as well as the improvement of the vascularization.

P–600 Ovarian function following intraovarian injection of autologous platelet rich plasma (APRP) in women with low functional ovarian reserve

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
D Barad ◽  
S K Darmon ◽  
A Benor ◽  
N Gleicher
Keyword(s):  
Growth Factors ◽  
Ovarian Reserve ◽  
Ovarian Function ◽  
Endometrial Thickness ◽  
Platelet Rich Plasma ◽  
Controlled Trial ◽  
Autologous Blood ◽  
Poor Response ◽  
Medical Specialties ◽  
Menstruating Women

Abstract Study question Does exposure of ovaries to autologous growth factors in platelet rich plasma (APRP) affect the pituitary ovarian axis? Summary answer Within 60 days after injection, growing follicle numbers and estrogen levels increased, though FSH did not change, with effects most pronounced in still menstruating women. What is known already APRP is extracted from a patient’s autologous blood and delivers growth factors. It is widely used in several medical specialties and has in infertility practice been reported to increase folicle/egg numbers if injected into ovaries and improve endometrial thickness/implantation if used for perfusion of the endometrium. Study design, size, duration Prospective observational cohort study of women with low functional ovarian reserve, followed for 60 days after subcortical injection of ovaries. Participants/materials, setting, methods 44 women with prior poor response to ovulation induction, FSH &gt; 12 mIU/mL and AMH &lt; 1.0 ng/mL. APRP was prepared using Regen Lab PRP Kit which is approved by the US-FDA. 1.0–1.5 ml of PRP was injected into the cortex of each ovary divided among 7 to 10 injection sites. Participants were followed every three days with monitoring for estradiol, FSH and follicle growth for the first two weeks after PRP and then weekly. Main results and the role of chance 21/43 patients still regularly menstruated (subgroup A, age 43.9 ± 5.1 years); 23/43 (subgroup B, age 42.6 ± 6.2 years) were amenorrheic for a median of 6 months. In A, AMH, FSH and estradiol were 0.18 ± 0.20 ng/mL, 37.5 ± 47.6 mIU/mL, and 100.2 ± 73.4 pg/mL, while in B they were 0.06 ± 0.11 ng/mL, 73.0 ± 44.8 mIU/mL and 66.7 ± 57.6 pg/mL. Following APRP, A-patients demonstrated increased estradiol to 211 ± 193.7 pg/mL (P = 0.029) while B-patients only demonstrated a trend to 98.1 ± 86.5 (P = 0.09). Among A patients, 14/21 (66.7%) entered IVF cycles and 5/21 (23.8%) reached retrieval. So-far 1 patients established an ongoing clinical pregnancy. Among B patients 8/23 (34.8%) entered IVF cycles and only 2/23 (8.7%) reached retrieval and none achieved pregnancy. Limitations, reasons for caution This observational study was only carried out to estimate possible effects of APRP treatments. Based on these observations, we are now conducting a randomized controlled trial, limited to cycling women under age 45 years [registration # NCT04278313]. Wider implications of the findings: PRP appears to have limited ability to affect ovarian reserve of older, and especially amenorrheic women. It may, however, exert more favorable effects on still menstruating women. Promotion of APRP treatment as “ovarian rejuvenation,” however, appears to be an inappropriate choice of words. Trial registration number N/A

scholarly journals Ovarian Rejuvenation Using Autologous Platelet-Rich Plasma

Endocrines ◽  
2021 ◽  
Vol 2 (1) ◽  
pp. 15-27
Author(s):  
Tuyen Kim Cat Vo ◽  
Yuka Tanaka ◽  
Kazuhiro Kawamura
Keyword(s):  
Growth Factors ◽  
Platelet Rich Plasma ◽  
Controlled Trial ◽  
Infertility Treatment ◽  
Published Data ◽  
High Concentration ◽  
Treatment Needs ◽  
Initial Report ◽  
Plasma Fraction ◽  
Autologous Platelet Rich Plasma

Advanced maternal age is associated with the natural oocyte depletion, leading to low oocyte yield, high infertility treatment cancellation rates, and eventual decreases in pregnancy rates. Various innovative interventions have been introduced to improve the outcome of infertility treatment for aging patients. Numerous published data demonstrated that early follicle development was regulated by intraovarian growth factors through autocrine or paracrine mechanisms. Platelet-rich plasma (PRP), a plasma fraction of peripheral blood with a high concentration of platelets, has been implemented in regenerative medicine in the last decade. The plasma contains a variety of growth factors that were suggested to be able to enhance angiogenesis regeneration and the cell proliferation process. The initial report showed that an intraovarian injection of PRP improved the hormonal profile and increased the number of retrieved oocytes in patients with diminished ovarian reserve. Subsequently, several studies with larger sample sizes have reported that this approach resulted in several healthy live births with no apparent complications. However, the use of ovarian PRP treatment needs to be fully investigated, because no randomized controlled trial has yet been performed to confirm its efficacy.

Autologous platelet-rich plasma versus readymade growth factors in skin rejuvenation: A split face study

2017 ◽  
Vol 16 (2) ◽  
pp. 258-264 ◽  
Author(s):  
Heba I Gawdat ◽  
Amira M Tawdy ◽  
Rehab A Hegazy ◽  
Mohga M Zakaria ◽  
Ryham S Allam
Keyword(s):  
Growth Factors ◽  
Platelet Rich Plasma ◽  
Skin Rejuvenation ◽  
Autologous Platelet Rich Plasma ◽  
Autologous Platelet ◽  
Split Face

scholarly journals Evaluation of graft uptake using temporalis fascia and cartilage perichondrium supplemented with autologous platelet rich plasma in tympanoplasty

2021 ◽  
Vol 7 (2) ◽  
pp. 308
Author(s):  
N. R. Ankle ◽  
Rajesh Radhakrishna Havaldar ◽  
Sweta Sinha
Keyword(s):  
Growth Factors ◽  
Mechanical Stability ◽  
Platelet Rich Plasma ◽  
Donor Site ◽  
Chronic Otitis Media ◽  
Temporalis Fascia ◽  
Autologous Platelet Rich Plasma ◽  
Operative Assessment ◽  
Autologous Platelet ◽  
And Cartilage

<p class="abstract"><strong>Background:</strong> Tympanoplasty involves grafting the perforation of the tympanic membrane with materials such as temporalis fascia, cartilage perichondrium, periosteum, vein, fat etc. Both temporalis fascia and cartilage perichondrium are easy to harvest with minimum donor site complications and both have been used extensively in tympanoplasty. Platelet rich plasma aids as an adhesive and supplements healing by providing growth factors. Till date there is scarcity of literature comparing the healing outcome of both cartilage perichondrium and temporalis fascia supplemented with platelet rich plasma. Hence, in this study we are comparing cartilage perichondrium supplemented with platelet rich plasma and temporalis fascia supplemented with platelet rich plasma.  </p><p class="abstract"><strong>Methods:</strong> An observational study involving 60 patients was done. Patients with chronic otitis media were evaluated by otoendoscopy to assess the ear and were categorised into 2 groups which received temporalis fascia and cartilage perichondrium respectively. All cases were supplemented with platelet rich plasma. Post-operative assessment was done by otoendoscopy.   </p><p class="abstract"><strong>Results:</strong> Total 21 patients received temporalis fascia and 39 patients received cartilage perichondrium. At the end of 6 weeks the graft site appeared unhealthy in 6.66% cases who received temporalis fascia and 1.66% in those who received cartilage perichondrium.  </p><p class="abstract"><strong>Conclusions:</strong> We found that cartilage perichondrium supplemented with platelet rich plasma had a better uptake after 6 weeks due to its superior mechanical stability. The results are more rewarding than the use of temporalis fascia with platelet rich plasma.</p>

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