scholarly journals Influence of the fungicide TMTD as a stress factor on the ultrastructure of pea (Pisum sativum L.) symbiotic nodules

2020 ◽  
Author(s):  
A. P. Gorshkov ◽  
A. V. Tsyganova ◽  
V. E. Tsyganov
Keyword(s):  
Pisum Sativum ◽  
Stress Factor ◽  
Ultrastructural Organization ◽  
Wild Type ◽  
Negative Effects ◽  
Tetramethylthiuram Disulfide ◽  
Pisum Sativum L ◽  
Wild Type Line

The negative effects of tetramethylthiuram disulfide (TMTD) on the ultrastructural organization of nodules of pea wild-type line SGE were studied.

scholarly journals The Fungicide Tetramethylthiuram Disulfide Negatively Affects Plant Cell Walls, Infection Thread Walls, and Symbiosomes in Pea (Pisum sativum L.) Symbiotic Nodules

Plants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1488
Author(s):  
Artemii P. Gorshkov ◽  
Anna V. Tsyganova ◽  
Maxim G. Vorobiev ◽  
Viktor E. Tsyganov
Keyword(s):  
Pisum Sativum ◽  
Cell Walls ◽  
Infection Thread ◽  
Nodule Development ◽  
Starch Accumulation ◽  
Ultrastructural Organization ◽  
Negative Effects ◽  
Tetramethylthiuram Disulfide ◽  
Transmission Electron ◽  
Nodule Senescence

In Russia, tetramethylthiuram disulfide (TMTD) is a fungicide widely used in the cultivation of legumes, including the pea (Pisum sativum). Application of TMTD can negatively affect nodulation; nevertheless, its effect on the histological and ultrastructural organization of nodules has not previously been investigated. In this study, the effect of TMTD at three concentrations (0.4, 4, and 8 g/kg) on nodule development in three pea genotypes (laboratory lines Sprint-2 and SGE, and cultivar ‘Finale’) was examined. In SGE, TMTD at 0.4 g/kg reduced the nodule number and shoot and root fresh weights. Treatment with TMTD at 8 g/kg changed the nodule color from pink to green, indicative of nodule senescence. Light and transmission electron microscopy analyses revealed negative effects of TMTD on nodule structure in each genotype. ‘Finale’ was the most sensitive cultivar to TMTD and Sprint-2 was the most tolerant. The negative effects of TMTD on nodules included the appearance of a senescence zone, starch accumulation, swelling of cell walls accompanied by a loss of electron density, thickening of the infection thread walls, symbiosome fusion, and bacteroid degradation. These results demonstrate how TMTD adversely affects nodules in the pea and will be useful for developing strategies to optimize fungicide use on legume crops.

scholarly journals Growth, seed development and genetic analysis in wild type and Def mutant of Pisum sativum L

2011 ◽  
Vol 4 (1) ◽  
pp. 489 ◽  
Author(s):  
Kwadwo Ayeh ◽  
YeonKyeong Lee ◽  
Mike J Ambrose ◽  
Anne Hvoslef-Eide
Keyword(s):  
Pisum Sativum ◽  
Genetic Analysis ◽  
Seed Development ◽  
Wild Type ◽  
Pisum Sativum L

scholarly journals Regulation of the Later Stages of Nodulation Stimulated by IPD3/CYCLOPS Transcription Factor and Cytokinin in Pea Pisum sativum L.

Plants ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 56
Author(s):  
Elizaveta S. Rudaya ◽  
Polina Yu. Kozyulina ◽  
Olga A. Pavlova ◽  
Alexandra V. Dolgikh ◽  
Alexandra N. Ivanova ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Comparative Analysis ◽  
Pisum Sativum ◽  
Nodule Development ◽  
Rna Seq ◽  
Wild Type ◽  
Development Stages ◽  
Pisum Sativum L ◽  
Pea Mutant

The IPD3/CYCLOPS transcription factor was shown to be involved in the regulation of nodule primordia development and subsequent stages of nodule differentiation. In contrast to early stages, the stages related to nodule differentiation remain less studied. Recently, we have shown that the accumulation of cytokinin at later stages may significantly impact nodule development. This conclusion was based on a comparative analysis of cytokinin localization between pea wild type and ipd3/cyclops mutants. However, the role of cytokinin at these later stages of nodulation is still far from understood. To determine a set of genes involved in the regulation of later stages of nodule development connected with infection progress, intracellular accommodation, as well as plant tissue and bacteroid differentiation, the RNA-seq analysis of pea mutant SGEFix--2 (sym33) nodules impaired in these processes compared to wild type SGE nodules was performed. To verify cytokinin’s influence on late nodule development stages, the comparative RNA-seq analysis of SGEFix--2 (sym33) mutant plants treated with cytokinin was also conducted. Findings suggest a significant role of cytokinin in the regulation of later stages of nodule development.

scholarly journals A Non-Rogue Mutant Line Induced by ENU Mutagenesis in Paramutated Rogue Peas (Pisum sativum L.) Is Still Sensitive to the Rogue Paramutation

Genes ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1680
Author(s):  
Ricardo Pereira ◽  
José M. Leitão
Keyword(s):  
Dna Methylation ◽  
Pisum Sativum ◽  
Positional Cloning ◽  
Methylation Status ◽  
Mutant Line ◽  
Wild Type ◽  
Enu Mutagenesis ◽  
Naturally Occurring ◽  
Pisum Sativum L ◽  
Epigenetic Phenomenon

The spontaneously emerging rogue phenotype in peas (Pisum sativum L.), characterized by narrow and pointed leaf stipula and leaflets, was the first identified case of the epigenetic phenomenon paramutation. The crosses of homozygous or heterozygous (e.g., F1) rogue plants with non-rogue (wild type) plants, produce exclusively rogue plants in the first and all subsequent generations. The fact that the wild phenotype disappears forever, is in clear contradiction with the Mendelian rules of inheritance, a situation that impedes the positional cloning of genes involved in this epigenetic phenomenon. One way of overcoming this obstacle is the identification of plant genotypes harboring naturally occurring or artificially induced neutral alleles, non-sensitive to paramutation. So far, such alleles have never been described for the pea rogue paramutation. Here, we report the induction via 1-ethyl-1-nitrosourea (ENU) mutagenesis of a non-rogue revertant mutant in the rogue cv. Progreta, and the completely unusual fixation of the induced non-rogue phenotype through several generations. The reversion of the methylation status of two previously identified differentially methylated genomic sequences in the induced non-rogue mutant, confirms that the rogue paramutation is accompanied by alterations in DNA methylation. Nevertheless, unexpectedly, the induced non-rogue mutant showed to be still sensitive to paramutation.

scholarly journals Organization of the endoplasmic reticulum in cells of effective and ineffective pea nodules (Pisum sativum L.)

2019 ◽  
Vol 17 (4) ◽  
pp. 5-14
Author(s):  
Anna V. Tsyganova ◽  
Viktor E. Tsyganov
Keyword(s):  
Endoplasmic Reticulum ◽  
Pisum Sativum ◽  
Structural Changes ◽  
Nodule Development ◽  
Wild Type ◽  
Meristematic Cells ◽  
Pisum Sativum L ◽  
Transmission Electron ◽  
Infected Cells ◽  
Membrane Bound

Background. The endoplasmic reticulum (ER) is the largest membrane-bound organelle, which plays an important role in the functioning of a plant cell and participates in its differentiation. Materials and methods. Using the methods of transmission electron microscopy, the morphological features and dynamics of structural changes in the ER in symbiotic nodules of pea (Pisum sativum L.) wild-type and mutants blocked at different stages of nodule development were studied. Results. ER developed from a network of individual tubules in meristematic cells, to a developed network of cisterns around the nucleus and plasmalemma, and a network of granular and smooth tubules accompanying infection structures in colonized and infected cells and symbiosomes in infected cells. Conclusions. A correlation was found between the level of development of the ER network and the degree of bacteroid differentiation.

scholarly journals Characterization and structural analysis of wild type and a non-abscission mutant at the development funiculus (Def) locus in Pisum sativum L

2009 ◽  
Vol 9 (1) ◽  
pp. 76 ◽  
Author(s):  
Kwadwo Ayeh ◽  
YeonKyeong Lee ◽  
Mike J Ambrose ◽  
Anne Hvoslef-Eide
Keyword(s):  
Structural Analysis ◽  
Pisum Sativum ◽  
Wild Type ◽  
Pisum Sativum L

Ultrastructural organization of chloroplast membranes in mutants of Pisum sativum L. with impaired activity in the photosystems

Planta ◽  
1981 ◽  
Vol 151 (6) ◽  
pp. 512-524 ◽  
Author(s):  
V. I. Popov ◽  
D. N. Matorin ◽  
S. V. Gostimsky ◽  
S. V. Tageeva ◽  
B. L. Allakhverdov
Keyword(s):  
Pisum Sativum ◽  
Ultrastructural Organization ◽  
Chloroplast Membranes ◽  
Pisum Sativum L

scholarly journals SEED GERMINATION OF PEA (Pisum Sativum, L) UNDER DIFFERENT BORON CONCENTRATION LEVELS

Irriga ◽  
1998 ◽  
Vol 3 (1) ◽  
pp. 20-23 ◽  
Author(s):  
Maria Dolores Barbosa Lima
Keyword(s):  
Seed Germination ◽  
Pisum Sativum ◽  
Boron Concentration ◽  
Control Treatment ◽  
Level Control ◽  
Negative Effects ◽  
Pisum Sativum L ◽  
The Media ◽  
Caixa Postal ◽  
Concentration Levels

SEED GERMINATION OF PEA (Pisum Sativum, L) UNDER DIFFERENT BORON CONCENTRATION LEVELS  Maria Dolores Barbosa LimaEscola Superior de Ciências Agrárias de Rio Verde - ESUCARVCampus Universitário - Caixa Postal 10475901-970 - Rio Verde , Goiás  1 ABSTRACT                 This experiment attempted to establish the effect of irrigation with water containing different concentration of boron on the germination of pea (Pisum sativum L.), selected as a typical example of dicotyledon plants. 25 seeds were planted in aluminum trays bedded with a layer of cotton of a depth 1 cm, as a germination medium. The media was wetted to saturation with waters of varying boron concentration starting from zero B level (control treatment) up to the highest concentration of 20 (mg.dm-3) B. Germination of peas was not affected by boron concentrations up to 8 (mg.dm-3), and only slightly reduced as 20 (mg.dm-3). Once germinated, the seedling were smaller, took longer to develop and already showed toxicity symptoms when boron was applied in large doses. Hence, large amounts of boron seem to have little influence on germination, but distinct negative effects on the development of plants at the seedling stage. Keywords: irrigation, germination, boron, pea.

Development of Erysiphe pisi (Powdery Mildew) on Normal and Albino Mutants of Pea (Pisum sativum L.)

2000 ◽  
Vol 148 (11-12) ◽  
pp. 591-595 ◽  
Author(s):  
U. P. Singh ◽  
B. Prithiviraj ◽  
B. K. Sarma
Keyword(s):  
Powdery Mildew ◽  
Pisum Sativum ◽  
Erysiphe Pisi ◽  
Pisum Sativum L

Mitigation Effects of 24-Epibrassinolide and Thiourea in Field Pea (Pisum sativum L.) under Drought Stress

2018 ◽  
Vol 34 (2) ◽  
pp. 229-235 ◽  
Author(s):  
Prachi Garg ◽  
◽  
A. Hemantaranjan ◽  
Jyostnarani Pradhan ◽  
◽  
...  
Keyword(s):  
Drought Stress ◽  
Pisum Sativum ◽  
Field Pea ◽  
Pisum Sativum L
Sign in / Sign up

Export Citation Format

Share Document