scholarly journals Biochemical and Culture-Based Approaches to Identification in the Diagnostic Microbiology Laboratory

2020 ◽  
pp. ascls.119.001875
Author(s):  
S. Travis Altheide
Keyword(s):  
Microbiology Laboratory ◽  
Diagnostic Microbiology

Usefulness of a Multiplex PCR for Detection of Diarrheagenic Escherichia coli in a Diagnostic Microbiology Laboratory Setting

2016 ◽  
Vol 1 (2) ◽  
pp. 38-42 ◽  
Author(s):  
Khairun Nessa ◽  
Dilruba Ahmed ◽  
Johirul Islam ◽  
FM Lutful Kabir ◽  
M Anowar Hossain
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Clinical Laboratory ◽  
Proteinase K ◽  
Microbiology Laboratory ◽  
Pcr Assay ◽  
E Coli ◽  
Diarrheagenic Escherichia Coli ◽  
Multiplex Pcr Assay ◽  
Diagnostic Microbiology

A multiplex PCR assay was evaluated for diagnosis of diarrheagenic Escherichia coli in stool samples of patients with diarrhoea submitted to a diagnostic microbiology laboratory. Two procedures of DNA template preparationproteinase K buffer method and the boiling method were evaluated to examine isolates of E. coli from 150 selected diarrhoeal cases. By proteinase K buffer method, 119 strains (79.3%) of E. coli were characterized to various categories by their genes that included 55.5% enteroaggregative E. coli (EAEC), 18.5% enterotoxigenic E. coli (ETEC), 1.7% enteropathogenic E. coli (EPEC), and 0.8% Shiga toxin-producing E. coli (STEC). Although boiling method was less time consuming (<24 hrs) and less costly (<8.0 US $/ per test) but was less efficient in typing E. coli compared to proteinase K method (41.3% vs. 79.3% ; p<0.001). The sensitivity and specificity of boiling method compared to proteinase K method was 48.7% and 87.1% while the positive and negative predictive value was 93.5% and 30.7%, respectively. The majority of pathogenic E. coli were detected in children (78.0%) under five years age with 53.3% under one year, and 68.7% of the children were male. Children under 5 years age were frequently infected with EAEC (71.6%) compared to ETEC (24.3%), EPEC (2.7%) and STEC (1.4%). The multiplex PCR assay could be effectively used as a rapid diagnostic tool for characterization of diarrheagenic E. coli using a single reaction tube in the clinical laboratory setting.Bangladesh J Med Microbiol 2007; 01 (02): 38-42

Use of the EntericBio Gastro Panel II in a diagnostic microbiology laboratory: challenges and opportunities

Pathology ◽  
2017 ◽  
Vol 49 (4) ◽  
pp. 419-422 ◽  
Author(s):  
Gary McAuliffe ◽  
Liselle Bissessor ◽  
Deborah Williamson ◽  
Sharon Moore ◽  
Janet Wilson ◽  
...  
Keyword(s):  
Microbiology Laboratory ◽  
Challenges And Opportunities ◽  
Diagnostic Microbiology

scholarly journals Laboratory diagnosis of human infections transmitted by ticks, fleas, mites and lice in Australia

2018 ◽  
Vol 39 (4) ◽  
pp. 182
Author(s):  
John Stenos ◽  
Stephen R Graves
Keyword(s):  
Fold Recognition ◽  
Laboratory Diagnosis ◽  
Nucleic Acid Amplification ◽  
Microbiology Laboratory ◽  
Human Pathogens ◽  
Diagnostic Assays ◽  
Wide Range ◽  
Vector Borne ◽  
Diagnostic Microbiology ◽  
Human Infections

A wide range of human pathogens (viruses, bacteria, protozoa) are transmitted by ticks, fleas, mites and lice worldwide. Some of these infections occur in Australia1, whereas others appear to be absent, although they may occur in returned travellers. The key to diagnosis is two-fold: recognition of the possibility of a vector-borne infection by the treating doctor and confirmation of the diagnosis in a diagnostic, microbiology laboratory. Laboratory diagnostic assays include culture (used rarely), nucleic acid amplification (used increasingly) and serology (used often).

scholarly journals Methicillin-resistant Staphylococcus aureus in the community in West Essex

2005 ◽  
Vol 134 (2) ◽  
pp. 301-305 ◽  
Author(s):  
S. MILLERSHIP ◽  
J. HARRIS ◽  
N. BATCHELOR
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Clinical Presentation ◽  
Care Homes ◽  
Microbiology Laboratory ◽  
Local Hospital ◽  
Methicillin Resistant ◽  
Diagnostic Microbiology ◽  
Hospital Acquired ◽  
Hospital Contact

The study was undertaken in response to local concerns of a rising number of community-acquired methicillin-resistant Staphylococcus aureus (MRSA) infections. All patients resident in the community in Essex at the time of diagnosis with a first isolate of MRSA between 1994 and 2001 identified by the diagnostic microbiology laboratory were included. Although the annual incidence rose throughout the study period the majority of those with MRSA (82%) had had hospital contact within the last 5 years. The subset of patients without hospital contact had a median age of 79·5 years, 63% were female and 32% were residents of care homes. Carriage in this population was detected for up to 41 months post-diagnosis. Neither antibiogram nor clinical presentation distinguished these isolates from local hospital-acquired strains.

scholarly journals Outbreak Investigation Using High-Throughput Genome Sequencing within a Diagnostic Microbiology Laboratory

2013 ◽  
Vol 51 (5) ◽  
pp. 1396-1401 ◽  
Author(s):  
N. L. Sherry ◽  
J. L. Porter ◽  
T. Seemann ◽  
A. Watkins ◽  
T. P. Stinear ◽  
...  
Keyword(s):  
Genome Sequencing ◽  
High Throughput ◽  
Outbreak Investigation ◽  
Microbiology Laboratory ◽  
Diagnostic Microbiology
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