scholarly journals Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Requirements of Blood Agar for Diagnostic Microbiology Laboratory Tests

PLoS ONE ◽  
2009 ◽  
Vol 4 (7) ◽  
pp. e6141 ◽  
Author(s):  
Ellen Yeh ◽  
Benjamin A. Pinsky ◽  
Niaz Banaei ◽  
Ellen Jo Baron
Keyword(s):  
Laboratory Tests ◽  
Blood Agar ◽  
Microbiology Laboratory ◽  
Hair Sheep ◽  
Sheep Blood ◽  
Diagnostic Microbiology

Usefulness of a Multiplex PCR for Detection of Diarrheagenic Escherichia coli in a Diagnostic Microbiology Laboratory Setting

2016 ◽  
Vol 1 (2) ◽  
pp. 38-42 ◽  
Author(s):  
Khairun Nessa ◽  
Dilruba Ahmed ◽  
Johirul Islam ◽  
FM Lutful Kabir ◽  
M Anowar Hossain
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Clinical Laboratory ◽  
Proteinase K ◽  
Microbiology Laboratory ◽  
Pcr Assay ◽  
E Coli ◽  
Diarrheagenic Escherichia Coli ◽  
Multiplex Pcr Assay ◽  
Diagnostic Microbiology

A multiplex PCR assay was evaluated for diagnosis of diarrheagenic Escherichia coli in stool samples of patients with diarrhoea submitted to a diagnostic microbiology laboratory. Two procedures of DNA template preparationproteinase K buffer method and the boiling method were evaluated to examine isolates of E. coli from 150 selected diarrhoeal cases. By proteinase K buffer method, 119 strains (79.3%) of E. coli were characterized to various categories by their genes that included 55.5% enteroaggregative E. coli (EAEC), 18.5% enterotoxigenic E. coli (ETEC), 1.7% enteropathogenic E. coli (EPEC), and 0.8% Shiga toxin-producing E. coli (STEC). Although boiling method was less time consuming (<24 hrs) and less costly (<8.0 US $/ per test) but was less efficient in typing E. coli compared to proteinase K method (41.3% vs. 79.3% ; p<0.001). The sensitivity and specificity of boiling method compared to proteinase K method was 48.7% and 87.1% while the positive and negative predictive value was 93.5% and 30.7%, respectively. The majority of pathogenic E. coli were detected in children (78.0%) under five years age with 53.3% under one year, and 68.7% of the children were male. Children under 5 years age were frequently infected with EAEC (71.6%) compared to ETEC (24.3%), EPEC (2.7%) and STEC (1.4%). The multiplex PCR assay could be effectively used as a rapid diagnostic tool for characterization of diarrheagenic E. coli using a single reaction tube in the clinical laboratory setting.Bangladesh J Med Microbiol 2007; 01 (02): 38-42

Rapid Streptococcal Tests

PEDIATRICS ◽  
1996 ◽  
Vol 97 (2) ◽  
pp. 288-288
Author(s):  
S. DUBOSE RAVENEL ◽  
GREGORY CARL ELLIS ◽  
WILLIAM N. MICHAL
Keyword(s):  
Blood Culture ◽  
Sensitivity And Specificity ◽  
Sheep Blood Agar ◽  
Blood Agar ◽  
Practice Setting ◽  
Throat Culture ◽  
Culture Techniques ◽  
Sheep Blood ◽  
Office Practice ◽  
Important Study

Roddey et al have reported an important study on the sensitivity and specificity of the Strep A OIA test compared with two culture techniques—5% sheep blood agar and Todd-Hewitt broth—in an office practice setting. They found the sensitivity and specificity of OIA as compared with sheep blood culture to be 91.4% and 95.6%, and compared with the broth method, 90.4% and 94.1%, respectively. They conclude that the OIA method is preferable for the majority of their patients, but recommend a throat culture be performed in cases with a negative OIA test.

scholarly journals 980. Using Videoconferencing with the Clinical Microbiology Lab to Enhance Medical Student Learning During Clinical Rotations

2018 ◽  
Vol 5 (suppl_1) ◽  
pp. S41-S41
Author(s):  
Peter Chin-Hong ◽  
Brian Schwartz
Keyword(s):  
Medical Student ◽  
Medical Students ◽  
Focus Groups ◽  
Student Learning ◽  
Laboratory Tests ◽  
Clinical Microbiology ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory ◽  
Clinical Workflow ◽  
Medical Student Learning

Abstract Background Preclinical medical student learning in the microbiology laboratory traditionally focuses on enhancing understanding of microbiology but less on understanding clinical workflow. During a novel course that revisited foundational sciences during clerkships, we designed a virtual microbiology laboratory session to enhance understanding and familiarity with clinical workflow on testing of patient samples. Methods The virtual microbiology laboratory sessions were conducted twice in 2018, each including 80 third-year medical students. Clinical cases were used to build upon foundational knowledge. We live-streamed video and audio content from the clinical microbiology laboratory to a remote classroom via the Zoom videoconferencing platform. We conducted the session as a tour and lively interview with microbiology staff who explained the processing as well as diagnostic testing Methods. Students were able to ask questions. To evaluate the sessions we (1) distributed a quantitative survey using a 5-point Likert scale (5 = strongly agree) and (2) conducted focus groups with learners. Qualitative data were analyzed using open and axial coding. Results In a questionnaire administered to 160 students, 74% of respondents agreed that the technical aspects and faculty in the session provided the feeling on a “hands-on” tour. Of the respondents, 58% reported that they would be more likely to contact the microbiology laboratory team for help in ordering or interpreting various laboratory tests. In focus groups, learner reflections reinforced the ability of this format to ensure standardization with each student getting to clearly see the demonstration and hear instructor perspectives. Students also appreciated the linear approach of following a specimen from arrival to the laboratory, a better understanding of the laboratory staff and their roles in performing and interpreting laboratory tests. The live feed could be enhanced further by better audio and video synchronization and by reducing ambient noise. Conclusion Videoconferencing with the clinical microbiology laboratory can be used to effectively teach microbiology and infectious diseases content to advanced medical students. Whether this exposure to the microbiology laboratory can enhance patient care outcomes requires further study. Disclosures All authors: No reported disclosures.

Characterization and antibiogram of enteropathogenic Escherichia coli isolated from diarrhoeic and non-diarrhoeic dogs in South Bengal, India

2015 ◽  
Author(s):  
A. Banik ◽  
D. P. Isore ◽  
S. N. Joardar ◽  
K. Batabyal ◽  
S. Dey
Keyword(s):  
Escherichia Coli ◽  
Nalidixic Acid ◽  
West Bengal ◽  
Sheep Blood Agar ◽  
Blood Agar ◽  
Enteropathogenic Escherichia Coli ◽  
Highly Pathogenic ◽  
E Coli ◽  
Sheep Blood ◽  
Common Serotypes

Diarrhoea in canines is mainly caused by Escherichia coli which can be fatal also. To understand the depth of this infection, a study was undertaken to detect E. coli isolates from diarrhoeic and non-diarrhoeic dogs in Southern part of West Bengal. A total of 112 canine samples were tested during May to September 2012 revealing approx 63.4% (71) samples positive for Escherichia coli. The most common serotypes were O8 (23.9%) followed by O157 (19.7%), O101 (16.9%), O26 (15.5%), O153 (12.7%) and O6 (11.3%). Among these O6, O8, O26 and O157 were highly pathogenic to mice causing almost 100% mortality within 24hrs of inoculation and were also detected to be haemolytic on sheep blood agar plates except serotype O8. These isolates were mostly sensitive to nalidixic acid (80.29%), cotrimoxazole (78.88%), ciprofloxacin (74.65%), colistin and ceftriaxone (both 71.83%) but were resistant to amikacin (97.18%), kanamycin (95.78%), cephalexin (92.96%) and enrofloxacin (84.51%).

scholarly journals Sterility Duration of Preprimed Extracorporeal Membrane Oxygenation Circuits

2018 ◽  
Vol 23 (4) ◽  
pp. 311-314 ◽  
Author(s):  
Vi Ean Tan ◽  
Alan T. Evangelista ◽  
Dominick M. Carella ◽  
Daniel Marino ◽  
Wayne S. Moore ◽  
...  
Keyword(s):  
Extracorporeal Membrane Oxygenation ◽  
Room Temperature ◽  
Agar Plate ◽  
Fungal Growth ◽  
Blood Agar ◽  
Blood Agar Plate ◽  
Macconkey Agar ◽  
Pilot Data ◽  
Sheep Blood ◽  
Membrane Oxygenation

OBJECTIVES There is a lack of standardization and supporting data regarding the duration preassembled and preprimed extracorporeal membrane oxygenation (ECMO) circuits are expected to be sterile. Therefore, the purpose of this study was to prospectively evaluate whether preassembled and preprimed ECMO circuits could maintain sterility for a period up to 65 days. DESIGN Four ECMO circuits (2 neonatal/pediatric¼” and 2 adolescent/adult ⅜ ”) were assembled and primed under sterile conditions and maintained at room temperature. Culture samples were obtained from each circuit and plated within 1 hour. Culture samples were obtained on day 0 when assembled and primed then every 5 days up to day 65. Samples were plated on several different media including the following: blood agar plate: trypticase soy agar with 5% sheep blood, MacConkey agar, and thioglycollate broth then incubated at 35°C for 3 days. RESULTS All cultures obtained from the priming solution from of the¼” and ⅜ ” ECMO circuits produced no microbial or fungal growth for the 65-day study period. CONCLUSION These pilot data suggest preprimed ECMO circuits may maintain sterility for a period up to 65 days. Additional studies evaluating a larger number of ECMO circuits are needed to confirm these findings.

scholarly journals Improved Detection of Streptococcus pneumoniae in Middle-Ear Fluid Cultures by Use of a Gentamicin-Containing Medium

1999 ◽  
Vol 37 (10) ◽  
pp. 3415-3416
Author(s):  
Nechama Peled ◽  
Pablo Yagupsky
Keyword(s):  
Streptococcus Pneumoniae ◽  
Middle Ear ◽  
Agar Medium ◽  
Blood Agar ◽  
Sheep Blood ◽  
Routine Blood ◽  
Middle Ear Fluid ◽  
Columbia Agar

The performance of Columbia agar medium with added sheep blood and gentamicin (CAG) for isolation of Streptococcus pneumoniaefrom middle-ear fluid cultures was compared to that of routine blood agar medium (BA). Of 238 pneumococcal isolates recovered, CAG plates detected 233 (97.9%) but BA plates detected only 208 (87.4%) (P < 0.001).

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