scholarly journals Evaluasi produksi dan kualitas inokulum fungi mikoriza arbuskula yang diproduksi dengan teknik hidroponik pada rumput Brachiaria decumbens var. mullato

2018 ◽  
Vol 16 (2) ◽  
pp. 10
Author(s):  
A T Aryanto ◽  
P DMH Karti ◽  
I Prihantoro

<p>ABSTRAK<br /><br />Pengembangan hijauan membutuhkan pupuk ramah lingkungan. Fungi Mikoriza Arbuskular (FMA) adalah asosiasi yang melibatkan jamur dan akar yang dianggap sebagai pupuk hayati untuk meningkatkan produktivitas tanaman dan toleran dari kondisi lingkungan. Ketersediaan FMA masih jarang, sehingga dibutuhkan produksi massal untuk dapat mendukung pengembangan hijauan. Tujuan dari penelitian ini adalah untuk menghasilkan inokulum AMF menggunakan sistem hidroponik dalam jumlah besar secara efektif. Penelitian ini dibagi menjadi 2 tahap. Rancangan Acak Kelompok Faktorial yang digunakan pada tahap pertama dengan faktor A adalah jenis sistem irigasi (Manual, Drip dan Nutrien Film Technique System (NFT)) dan B adalah larutan nutrisi (AB Mix dan Hyponex Red) dengan Pueraria javanica sebagai tanaman inang. Rancangan Acak Lengkap (RAL) dilakukan pada tahap kedua dengan menggunakan produksi inokulum FMA dari tahap pertama dengan Brachiaria decumbens var Mullato sebagai tanaman inang. Hasil penelitian menunjukkan bahwa interaksi antara sistem NFT dan AB Mix signifikan (P&lt;0,05) menghasilkan bahan kering tajuk, bahan kering akar dan spora paling tinggi. Semua tipe sistem irigasi dan nutrisi menunjukkan infeksi akar&gt;96%. FMA inokulasi di Brachiaria decumbes var Mulato signifikan(P &lt;0,05) pada bahan kering tajuk, kandungan N, kandungan P dan serapan P.</p><p><br />Kata kunci: Brachiaria decumbes, FMA, sistem Drip, sistem NFT, Pueraria javanica,</p><p><br />ABSTRACT</p><p>Forage mass production development requires environmental friendly fertilizer. Arbuscular Mycorrhizal Fungi (AMF) are mutualitic symbioses between plant and fungi that considered as natural biofertilizer with benefit to improve plant productivitity and environment stress tolerance. The availability of AMF is still low, so it takes mass production to be able to support forage development. The aim of the research was to produce AMF inoculum using hydroponic system in large quantities. This research divided into 2 stages. The factorial randomized block design was used for the first stage with A factor was type of irrigation system (Manual, Drip and Nutrient Film Technique System (NFT)) and B was the nutritional solution (AB Mix and Hyponex Red) using Pueraria javanica as host plant. Completely randomized design was conducted for the second stage by using AMF inoculum production from first stage using Brachiariadecumbens var Mullat as host plant. The best result was a combination beetwen NFT system and AB Mix significantly (P&lt;0.05) produce highest shoot dry matter, root dry matter and spore production. All type of irrigation system and nutrition showed root infection &gt;96%. AMF inoculation in Brachiariadecumbes var Mulato was significant different (P&lt;0.05) on shoot dry matter, N content, P content and P uptake.</p><p><br />Keywords: AMF, B. decumbes, Drip system, NFT system, P. javanica,</p>

2016 ◽  
Vol 40 (3) ◽  
pp. 203 ◽  
Author(s):  
Bambang Suwignyo ◽  
Bela Putra ◽  
Nafiatul Umami ◽  
Cahyo Wulandari ◽  
Ristianto Utomo

This study aimed to determine the effect of arbuscular mycorrhizal fungi (AMF) and phosphate (P) fertilizer on the nutrient content, phosphate uptake and in vitro digestibility of alfalfa (Medicago sativa L.).The research was conducted at green house of Forage and Pastures Science Laboratory, Faculty of Animal Science Universitas Gadjah Mada. The experiment was arranged in Completely Randomized Design using 3x4 factorial patterns with four replications. The first factor was dosage of phosphate fertilizer SP 36 (0, 60, and 120 kg/ha). Second factor was the dosage of AMF (0, 0.8, 1.6, and 2.4kg/ha). The variable measured was nutrient contents (crude protein, dry matter, and organic matter), total P uptake and dry matter and organic matter in vitro digestibility. The results showed that the interaction of AMF and P fertilizer had no significant effect on crude protein and total P uptake, but highly significant effect on the parameters of dry matter, organic matter and dry matter and organic matter in vitro digestibility. 


Revista CERES ◽  
2016 ◽  
Vol 63 (1) ◽  
pp. 86-94
Author(s):  
Dácio Jerônimo de Almeida ◽  
Walter Esfrain Pereira ◽  
Patrícia da Silva Alexandre ◽  
Járisson Cavalcante Nunes ◽  
Wagner de Melo Ferreira

ABSTRACT The indiscriminate use of mineral fertilizers in papaya orchards has increased production costs, and the use of arbuscular mycorrhizal fungi is a promising alternative to reduce such expenses. Therefore, the present research aimed at studying the efficiency of arbuscular mycorrhizal fungi (AMF) on dry matter and nutrient accumulation in Sunrise Solo papaya seedlings, by applying doses of P2O5 (triple superphosphate) that are harmful to the symbiosis. The experiment was carried out in a protected environment and was set up in a randomized block design with four replications, and consisted of four P2O5 doses (0, 672, 1386 and 2100 mg dm-3), three mycorrhizal fungi species (Gigaspora margarita, Entrophospora colombiana and Scutellospora heterogama) and the control treatment (mycorrhiza-free). Shoot and root dry matter as well as nitrogen, phosphorus and potassium contents in leaf and root tissues were assessed. Mycorrhizal inoculation promoted a 30% increase in shoot dry matter in relation to the control treatment. Mycorrhizal fungi promoted increases in leaf and root nitrogen content up to 672 mg dm-3 P2O5. Inoculation of E. colombiana favored the highest gains in root and shoot dry matter. P2O5 fertilization increased foliar and root phosphorus content.


2016 ◽  
Vol 6 (01) ◽  
pp. 5211 ◽  
Author(s):  
Sharanappa Jangandi ◽  
Chaitra B. Negalur* ◽  
Mr. Narayan ◽  
H. C. Lakshman

The influence of phosphate solubilizing bacterium (Bacillus polymyxa) and arbuscular mycorrhizal fungi (Rhizophagus fasciculatus) on growth and phosphorus uptake in Cajanus cajana were studied, in green house conditions. Experiments were carried out by using both sterilized and unsterilized garden soil (sandy loam). Mycorrhiza with P-solubilizing bacteria inoculated with sterilized soil produced significantly higher growth, dry matter and increase in nodule number and P uptake in shoot. Moderate or lower growth response was observed among the plants grown in unsterilized soil either PSB or AMF inoculation. On the contrary non-inoculated plants in sterilized garden soil did not showed meager growth and higher total P uptake. A synergistic effect was recorded with increased plant dry matter, nodule number and P uptake in the plants treated with both the inoculum and in sterilized soil.


2011 ◽  
Vol 46 (9) ◽  
pp. 1099-1104 ◽  
Author(s):  
João Ricardo Gonçalves de Oliveira ◽  
Thiago Alberto de Lima Morais ◽  
Natoniel Franklin de Melo ◽  
Adriana Mayumi Yano‑Melo

The objective of this work was to assess the potential of three isolates of arbuscular mycorrhizal fungi to promote growth of micropropagated plantlets of Tapeinochilos ananassae during acclimatization. The experiment was carried out in greenhouse, in a completely randomized block design, with four inoculation treatments: non‑inoculated control and plants inoculated with Glomus etunicatum, Acaulospora longula or Gigaspora albida, with ten replicates. After 90 days, the following parameters were evaluated: survival rate, height, leaf and tiller number, leaf area, fresh and dry biomass, contents of macro‑ and micronutrients in the root and shoot, glomerospore number, and mycorrhizal colonization. The survival percentage was 100%, except for plants inoculated with G. albida (80%). The isolate G. etunicatum is more suitable for plant development, since it improves survival, growth, dry matter production, nutritional status, and vigor of T. ananassae micropropagated plants.


2021 ◽  
Vol 134 ◽  
pp. 187-196
Author(s):  
M.J. Salomon ◽  
S.J. Watts-Williams ◽  
M.J. McLaughlin ◽  
C.J. Brien ◽  
N. Jewell ◽  
...  

2020 ◽  
Vol 3 (2) ◽  
pp. 56-64
Author(s):  
Rahayu Arraudah ◽  
Yudhy Harini Bertham ◽  
Hesti Pujiwati ◽  
Bambang Gonggo Murcitro ◽  
Entang Inoriah Sukarjo

Soybean is one of the most popular food crops for the community, but the needs for soybeans have not been fulfilled by soybean production. To meet the needs of soybeans, it is necessary to intensify agricultural land in Ultisol. This study aims to obtain the optimum concentration of humic acid and dosage of the Arbuscular Mycorrhizal Fungi (AMF) to increase soybean plants' production in Ultisols. This research was conducted from January to April 2020 in Beringin Raya Village, Muara Bangkahulu District, Bengkulu City, at an altitude of 10 m above sea level. The research design used a Randomized Complete Block Design (RCBD) two factors with three replications, arranged factorially in experimental units. The first factor is the concentration of humic acid, consisting of 4 levels: 0, 15, 30, and 45 mL L-1 . The second factor is the dose of AMF, consisted of 3 levels, namely: 0, 2.5, and 5 g plant-1. The results showed that the maximum soybean growth and yield in Ultisols were obtained from the humic acid concentration at 45 mL L-1 at the dose of AMF at 2.5 g plant-1 . The resulting production potential is 1.99 tons ha-1 . The administration of humic acid or AMF independently at this research stage had not yet given a maximum response to the growth and yield of soybean in Ultisol.


2021 ◽  
Vol 12 ◽  
Author(s):  
Ricardo Alexander Peña Venegas ◽  
Soon-Jae Lee ◽  
Moses Thuita ◽  
Deusdedit Peter Mlay ◽  
Cargele Masso ◽  
...  

A vast majority of terrestrial plants are dependent on arbuscular mycorrhizal fungi (AMF) for their nutrient acquisition. AMF act as an extension of the root system helping phosphate uptake. In agriculture, harnessing the symbiosis can potentially increase plant growth. Application of the AMF Rhizophagus irregularis has been demonstrated to increase the yields of various crops. However, there is a paradigm that AMF colonization of roots, as well as the plant benefits afforded by inoculation with AMF, decreases with increasing phosphorus (P) supply in the soil. The paradigm suggests that when fertilized with sufficient P, inoculation of crops would not be beneficial. However, the majority of experiments demonstrating the paradigm were conducted in sterile conditions without a background AMF or soil microbial community. Interestingly, intraspecific variation in R. irregularis can greatly alter the yield of cassava even at a full application of the recommended P dose. Cassava is a globally important crop, feeding 800 million people worldwide, and a crop that is highly dependent on AMF for P uptake. In this study, field trials were conducted at three locations in Kenya and Tanzania using different AMF and cassava varieties under different P fertilization levels to test if the paradigm occurs in tropical field conditions. We found that AMF colonization and inoculation responsiveness of cassava does not always decrease with an increased P supply as expected by the paradigm. The obtained results demonstrate that maximizing the inoculation responsiveness of cassava is not necessarily only in conditions of low P availability, but that this is dependent on cassava and fungal genotypes. Thus, the modeling of plant symbiosis with AMF under different P levels in nature should be considered with caution.


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