scholarly journals Application of Humic Acid and Arbuscular Mycorrhizal Fungi to Increase Growth and Yields of Soybean in Ultisol

2020 ◽  
Vol 3 (2) ◽  
pp. 56-64
Author(s):  
Rahayu Arraudah ◽  
Yudhy Harini Bertham ◽  
Hesti Pujiwati ◽  
Bambang Gonggo Murcitro ◽  
Entang Inoriah Sukarjo
Keyword(s):  
Humic Acid ◽  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Agricultural Land ◽  
Block Design ◽  
Arbuscular Mycorrhizal ◽  
Growth And Yield ◽  
Two Factors ◽  
Soybean Plants ◽  
Research Stage

Soybean is one of the most popular food crops for the community, but the needs for soybeans have not been fulfilled by soybean production. To meet the needs of soybeans, it is necessary to intensify agricultural land in Ultisol. This study aims to obtain the optimum concentration of humic acid and dosage of the Arbuscular Mycorrhizal Fungi (AMF) to increase soybean plants' production in Ultisols. This research was conducted from January to April 2020 in Beringin Raya Village, Muara Bangkahulu District, Bengkulu City, at an altitude of 10 m above sea level. The research design used a Randomized Complete Block Design (RCBD) two factors with three replications, arranged factorially in experimental units. The first factor is the concentration of humic acid, consisting of 4 levels: 0, 15, 30, and 45 mL L-1 . The second factor is the dose of AMF, consisted of 3 levels, namely: 0, 2.5, and 5 g plant-1. The results showed that the maximum soybean growth and yield in Ultisols were obtained from the humic acid concentration at 45 mL L-1 at the dose of AMF at 2.5 g plant-1 . The resulting production potential is 1.99 tons ha-1 . The administration of humic acid or AMF independently at this research stage had not yet given a maximum response to the growth and yield of soybean in Ultisol.

scholarly journals Physiological and biochemical responses of soybean plants inoculated with Arbuscular mycorrhizal fungi and Bradyrhizobium under drought stress

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Mohamed S. Sheteiwy ◽  
Dina Fathi Ismail Ali ◽  
You-Cai Xiong ◽  
Marian Brestic ◽  
Milan Skalicky ◽  
...  
Keyword(s):  
Drought Stress ◽  
Arbuscular Mycorrhizal Fungi ◽  
Secondary Metabolism ◽  
Mycorrhizal Fungi ◽  
Plant Biomass ◽  
Arbuscular Mycorrhizal ◽  
Growth And Yield ◽  
Relative Expression ◽  
Reverse Trend ◽  
Soybean Plants

Abstract Background The present study aims to study the effects of biofertilizers potential of Arbuscular Mycorrhizal Fungi (AMF) and Bradyrhizobium japonicum (B. japonicum) strains on yield and growth of drought stressed soybean (Giza 111) plants at early pod stage (50 days from sowing, R3) and seed development stage (90 days from sowing, R5). Results Highest plant biomass, leaf chlorophyll content, nodulation, and grain yield were observed in the unstressed plants as compared with water stressed-plants at R3 and R5 stages. At soil rhizosphere level, AMF and B. japonicum treatments improved bacterial counts and the activities of the enzymes (dehydrogenase and phosphatase) under well-watered and drought stress conditions. Irrespective of the drought effects, AMF and B. japonicum treatments improved the growth and yield of soybean under both drought (restrained irrigation) and adequately-watered conditions as compared with untreated plants. The current study revealed that AMF and B. japonicum improved catalase (CAT) and peroxidase (POD) in the seeds, and a reverse trend was observed in case of malonaldehyde (MDA) and proline under drought stress. The relative expression of the CAT and POD genes was up-regulated by the application of biofertilizers treatments under drought stress condition. Interestingly a reverse trend was observed in the case of the relative expression of the genes involved in the proline metabolism such as P5CS, P5CR, PDH, and P5CDH under the same conditions. The present study suggests that biofertilizers diminished the inhibitory effect of drought stress on cell development and resulted in a shorter time for DNA accumulation and the cycle of cell division. There were notable changes in the activities of enzymes involved in the secondary metabolism and expression levels of GmSPS1, GmSuSy, and GmC-INV in the plants treated with biofertilizers and exposed to the drought stress at both R3 and R5 stages. These changes in the activities of secondary metabolism and their transcriptional levels caused by biofertilizers may contribute to increasing soybean tolerance to drought stress. Conclusions The results of this study suggest that application of biofertilizers to soybean plants is a promising approach to alleviate drought stress effects on growth performance of soybean plants. The integrated application of biofertilizers may help to obtain improved resilience of the agro ecosystems to adverse impacts of climate change and help to improve soil fertility and plant growth under drought stress.

scholarly journals The Effects of Types of Manure and Mycorrhizal Applications on Sandy Soils on the Growth and Yield of Curly Red Chili (Capsicum annum L.)

2020 ◽  
Vol 35 (2) ◽  
pp. 258
Author(s):  
Sukmana Siswandana Putra ◽  
Eka Tarwaca Susila Putra ◽  
Jaka Widada
Keyword(s):  
Mycorrhizal Fungi ◽  
Block Design ◽  
Arbuscular Mycorrhizal ◽  
Fruit Production ◽  
Chicken Manure ◽  
Growth And Yield ◽  
Beach Sand ◽  
Market Demand ◽  
Soil Microbial ◽  
Two Factors

Market demand for chilies continues to increase, so efforts are needed to increase the productivity of the chili plants. One of the efforts is by intensifying production factors such as applying fertilizers on beach sand which contain low nutrients and organic matter so that manure input is needed and also utilizes soil microbial technology in the form of arbuscular mycorrhizal fungi which are relatively cheap economically and environmentally friendly. The purpose of this research was to study the effect of types of manure and mycorrhizal applications on the growth and yield of curly red chili. The research was arranged in a strip-plot Randomized Completely Block Design (RCBD) consisting of two factors with three replications. The first factor is the type of fertilizer (cow manure, chicken manure, guano fertilizer, quail manure and urea fertilizer) and the second factor is the application of mycorrhizae (with and without). This study concludes that quail manure increases plant height. The addition of chicken manure, guano fertilizer and quail manure multiplies the total fruit production. The application of mycorrhizae gives the same results on the growth and yield of chili.

scholarly journals Growth and Yield of Soybean with Application of Liquid Organic Fertilizer and Arbuscular Mycorrhizal Fungi in Ultisols

2021 ◽  
Vol 4 (1) ◽  
pp. 1-8
Author(s):  
Lisa Septiani ◽  
RR Yudhy Harini Bertham ◽  
Hesti Pujiwati ◽  
Bilman Wilman Simanihuruk
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Block Design ◽  
Organic Fertilizer ◽  
Arbuscular Mycorrhizal ◽  
The Body ◽  
Organic Fertilizers ◽  
Growth And Yield ◽  
Plant Weight ◽  
Four Levels

Soybean (Glycine max (L.) Merill) is the third most important food crop after rice and corn which contains protein and other nutrients essential for the body. Ultisol soil is a less fertile soil that has many limitations on its physical, chemical, and biological properties. Efforts that can be made include the use of liquid organic fertilizer (LOF) and arbuscular mycorrhizal fungi (AMF). This research was conducted from February to May 2020, in Beringin Raya, Muara Bangka Hulu District, Bengkulu City with an altitude of + 10 m above sea level. The purpose of this study was to explain the growth and yield of soybeans due to the application of liquid organic fertilizers and arbuscular mycorrhizal fungi in Ultisols. The research design used was a randomized complete block design (RCBD) with 2 factors with three replications. The first factor is the LOF dose which consists of four levels, namely: 0, 20, 40, and 60 mL L-1. The second factor is the AMF dose with three levels, namely; 0, 5, and 10 g plant-1. The results showed that there was no interaction between LOF and AMF. Giving a LOF concentration of 60 mL L-1 gave the highest yield on the growth and yield of soybeans, as well as the optimum concentration for seed/plant weight, which was 28.114 mL L-1, and the number of seeds was 37.589 mL L-1. AMF dosage of 10 g plant-1 gave the best growth and yield of soybean plants. 

scholarly journals Effects of selenium (Se) application and arbuscular mycorrhizal (AMF) inoculation on soybean (Glycine max) and forage grass (Urochloa decumbens) development in oxisol

2019 ◽  
Vol 13 ((03) 2019) ◽  
pp. 380-385 ◽  
Author(s):  
Soraya Marx Bamberg ◽  
Silvio Junio Ramos ◽  
Marco Aurelio Carbone Carneiro ◽  
José Oswaldo Siqueira
Keyword(s):  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Fold Increase ◽  
Tropical Soils ◽  
Fertilizer Application ◽  
Growth And Yield ◽  
Forage Grass ◽  
Urochloa Decumbens ◽  
Soybean Plants ◽  
Amf Inoculation

Fertilizer application can enhance the nutritional value of plants, such effects being influenced by the presence of arbuscular mycorrhizal fungi (AMF). Nutrients × AMF interactions are well-known for variety of elements but very little has been addressed on biofortification of selenium (Se) in plants grown in tropical soils. The purpose of this study was to evaluate the effect of Se application and AMF inoculation on growth and micronutrient contents on soybean plants as forage grass. The experiments were conducted in a completely randomized factorial design with five Se doses (0.0, 0.5, 1.0, 2.0 and 3.0 mg kg-1 for soybean plants, and 0.0, 0.5, 1.0, 3.0 and 6.0 mg kg-1 for forage plants), with and without AMF inoculation in three replicates. The results showed that soil Se had only slight effect on soybean growth but it caused a two-fold increase on grain yield. However, the growth of forage grass was enhanced by Se application when AMF was present. The AMF inoculation reduced benefit for soybean growth and yield but marked positive effect on forage grass at high doses of Se. Selenium contents in both plants were increased by its application in soil, being such effect proportional to soil applied doses. Selenium application and AMF inoculation had marked effects on micronutrients contents in both soybean plants and forage grass and they may contribute to Se and micronutrient biofortification.

scholarly journals INFLUENCE OF SOME LEGUMINOUS TREES AND ARBUSCULAR MYCORRHIZAL FUNGI ON THE PHYSIOLOGY AND YIELD OF DIOSCOREA ROTUNDATA -POIR

2020 ◽  
Author(s):  
Ezekiel Taiwo Afolayan
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Leucaena Leucocephala ◽  
Arbuscular Mycorrhizal ◽  
Gliricidia Sepium ◽  
Growth And Yield ◽  
Dioscorea Rotundata ◽  
Npk Fertilizers ◽  
Inorganic Fertilizers ◽  
Chlorophyll Contents

AbstractThis work compares the physiological and yield characteristics of white yam (Dioscorea rotundata – Poir) under Arbuscular mycorrhizal fungi (AMF) inoculation, green manures of Gliricidia sepium, Leucaena leucocephala and other soil amendments. The experiment was conducted on the plot of land that had been overcropped, located at the back of the male Hostel, Federal College of Education, Abeokuta, Ogun State, Nigeria. The land was cleared and heaped at 1m x 1m apart. The experimental design employed was a complete randomized design in 5 replicates. The treatments were Glomus deserticola (GD), Glomus fasciculatum (GF), Gliricidia sepium (GS), Leucaena leucocephala (LL), Poultry manure (PM) and NPK fertilizers. Soils were dug from the heaps, 20 g of the inoculums of AMF (GD/ GF) were poured into the dug hole, seeds were laid on it and covered with soil (for GD & GF treatments). Others were applied at one week after sprouting. Growth and yield Parameters were determined at harvest while relative water and chlorophyll contents were measured forth nightly from 10 weeks after treatment. Data obtained were subjected to ANOVA while means were separated by Duncan multiple range test at P> 0.05. Results showed that growth, yield and physiological characters were enhanced in GD, GD+GF, GS and PM treated plants more than in inorganic fertilizers treated plants. There was a positive significant relationship between white yam’s growth, physiology and tuber yield. The study justifies the use of plant/animal manures and Arbuscular mycorrhizal fungi in place of inorganic fertilizers.

scholarly journals Morphological and molecular identifications of three native arbuscular mycorrhizal fungi isolated from the rhizosphere of Elaeis guineensis and Jatropha curcas in Indonesia

2021 ◽  
Vol 22 (11) ◽  
Author(s):  
Maria Viva Rini ◽  
Fitri Yelli ◽  
Darwin Leonardo Tambunan ◽  
Inggar Damayanti
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Molecular Analysis ◽  
Jatropha Curcas ◽  
Mycorrhizal Fungi ◽  
Morphological Analysis ◽  
Agricultural Land ◽  
Elaeis Guineensis ◽  
Arbuscular Mycorrhizal ◽  
Fungal Colonization ◽  
Morphological Studies

Abstract. Rini MV, Yelli F, Tambunan DL, Damayanti I. 2021. Morphological and molecular identifications of three native arbuscular mycorrhizal fungi isolated from the rhizosphere of Elaeis guineensis and Jatropha curcas in Indonesia. Biodiversitas 22: 4940-4947. Molecular analysis has been widely used to provide more accurate identification within arbuscular mycorrhizal fungi (AMF) species than identification based on morphology. However, morphological analysis is essential for a basic preliminary of classification studies. Therefore, a study is needed to complete the identification of AMF isolates through morphological and molecular analyses. This research used three AMF isolates, namely MV 5, MV 17, and MV 18, which were isolated from Indonesian agricultural land. Spore-based taxonomy (shape, size, color, ornamentation, PVLG, and Melzer’s reaction) and fungal colonization on roots of maize trap plants were employed for the morphological studies. AMF species identification was performed using molecular analysis through nested-Polymerase Chain Reaction (PCR) to amplify a fragment of SSU rRNA followed by sequencing and phylogenetic tree construction. Morphological analysis showed that MV 5 had spores borne from the neck of the sporiferous saccule, MV 17 was found to have a bulbous suspensor without a germination shield, and MV 18 had spores borne from subtending hyphae. The SSUR rRNA analysis revealed that MV 5, MV 15, and MV 18 were identified as Acaulospora longula, Gigaspora margarita, and Glomus etunicatum, respectively. Both morphological and molecular methods demonstrated reliable and consistent results that complement AMF taxonomy studies.

scholarly journals MENINGKATKAN PERTUMBUHAN DAN HASIL PAKCOY (BRASSICA JUNCEARAPAL.) DENGAN MENGATUR DOSIS NUTRISI AB MIX AGRIFARM DAN UMUR BIBIT SECARA HIDROPONIK SISTEM NFT

GANEC SWARA ◽  
2019 ◽  
Vol 13 (2) ◽  
pp. 212
Author(s):  
I WAYAN SUKASANA ◽  
I NENGAH KARNATA ◽  
BUDI IRAWAN
Keyword(s):  
Combination Treatment ◽  
Agricultural Land ◽  
Block Design ◽  
Growth And Yield ◽  
Vegetable Crops ◽  
Randomized Block Design ◽  
Mustard Plant ◽  
Two Factors ◽  
Soil Media ◽  
Wet Weight

   Pakcoy is a type of mustard plant that is quite popular with the community, it is very suitable to be cultivated considering the need for vegetables is quite large. The community's need for fresh and pesticide-free vegetables is a choice now in line with the community's understanding of a healthy diet. Alternatives to fulfillment are chosen by planting with hydroponic media. Hydroponics is a substitute for soil media that can be used to grow vegetable crops. The presence of hydroponics is expected to be able to overcome the narrowing of agricultural land and reduce the use of pesticides considering that pesticides greatly affect human health and the environment.   This study aims to determine the effect of nutritional doses of AB mix agrifarm and age of seedlings and their interactions on the growth and yield of pakcoy plants, this study is carried out in the experimental garden of the Faculty of Agriculture, Tabanan University which began on 9 December 2018 until 12 January 2019.   The study used a Randomized Block Design (RBD) consisting of two factors. The first factor is the nutritional dose of AB mix agrifarm (N) which consists of three dose levels, namely the dose of 850 ppm, 1050 ppm and 1250 ppm. The second factor is the age of seedlings (U) which consists of two seedling age levels, namely the age of seedlings 1 week and 2 weeks. Each treatment is repeated four times.   The results of the study obtained the interaction of dose agrifarm treatment with seed age showed a very significant effect (p 1 0.01) on all parameters except for the parameters of the number of leaves aged 35 days old which showed no significant effect (p 5 0.05). The economical wet weight of plant-1 and BKO of the highest yield of 1-crop yield is produced in the combination treatment of N3U, which amounted to respectively 244.09 g and 78.10g or increased respectively by 57.78% and 293.85% compared to results the lowest produced by the combination treatment of N1U1 is 154.71g and 19.83g

scholarly journals Sunflower response to inoculation with single and mixed species of arbuscular mycorrhizal fungi: Agronomic characteristics

2019 ◽  
Vol 113 (2) ◽  
pp. 321
Author(s):  
Mazen IBRAHIM
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Glomus Intraradices ◽  
Arbuscular Mycorrhizal ◽  
Abundant Species ◽  
Growth And Yield ◽  
Agricultural Field ◽  
Mycorrhizal Dependency ◽  
Agronomic Characteristics ◽  
The Impact

The impact of indigenous arbuscular mycorrhizal fungi (AMF) on agronomic characteristics of sunflower (<em>Helianthus annuus</em> L.) was evaluated in a pot experiment. The indigenous AMF, including <em>Glomus intraradices, Glomus mosseae</em>, and <em>Glomus viscosum</em>, were isolated from an agricultural field in which cotton and sunflower plants were grown. The most abundant species (<em>G. viscosum</em>) was multiplied in a monospecific culture. Sunflower plants were inoculated with the mixture of three selected AMF species or solely with <em>G. viscosum</em>. The number of leaves, shoot length, head diameter, above ground biomass, and seeds mass were significantly higher in the plant inoculated with AMF mixture followed by individual inoculation with <em>G. viscosum</em> followed by the control. AMF mixture outperformed the <em>G. viscosumby</em> increasing mycorrhizal dependency and mycorrhizal inoculation effect of sunflower. The results indicate that AMF mixture could be considered as a good inoculum for improving growth and yield of sunflower in sustainable agriculture.

scholarly journals Influence of bioproducts and mycorrhizal fungi on the growth and yielding of sweet cherry trees

2020 ◽  
Vol 47 (No. 2) ◽  
pp. 122-129
Author(s):  
Sławomir Głuszek ◽  
Edyta Derkowska ◽  
Lidia Sas Paszt ◽  
Mirosław Sitarek ◽  
Beata Sumorok
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Sweet Cherry ◽  
Growth Parameters ◽  
Block Design ◽  
Organic Fertilizer ◽  
Arbuscular Mycorrhizal ◽  
Organic Fertilizers ◽  
Mineral Fertilizers ◽  
Cherry Trees

The experiment assessed the influence of various biofertilizers and biostimulants on the growth characteristics of the root system, its colonization by arbuscular mycorrhizal fungi and the yielding of sweet cherry trees in field conditions. The experiment, conducted in Pomological Orchard of Research Institute of Horticulture located in Skierniewice during 2011–2014, involved the use of a mycorrhizal substrate, organic fertilizers and biostimulant in randomised block design. The control combination consisted of plants fertilized with mineral fertilizers (NPK). The use of the organic fertilizer BF Ekomix in dose 100 g per tree each year in the spring significantly increased the number of root tips in comparison with the control trees. There was also a tendency for the roots to lengthen and increase their surface area under the influence of this biofertilizer. In addition, the inoculation of roots with the mycorrhizal substrate in dose 200 g per tree per year stimulated the colonization of the roots of sweet cherry trees by arbuscular mycorrhizal fungi, which in turn led to improved root growth parameters.

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