Evaluation of the Efficacy of Vitamin D Supplementation With Two Different Doses During Pregnancy on Maternal and Cord Blood Vitamin D Status, Metabolic, Inflammatory and Oxidative Stress Biomarkers, and Maternal and Neonatal Outcomes: a Study Protocol

Abstract Background Vitamin D deficiency during pregnancy is common and is likely to be associated with metabolic complications in the mother. The aim of this study was to assess the efficacy of two doses of vitamin D supplementation during pregnancy on maternal and cord blood vitamin D status and metabolic and oxidative stress biomarkers. Methods The eligible pregnant women (n = 84) invited to participate in the study and randomly allocated to one of the two supplementation groups (1000 IU/d vitamin D and 2000 IU/d). Biochemical assessments of mothers including serum concentrations of 25(OH)D, calcium, phosphate, iPTH, fasting serum sugar (FBS), insulin, triglyceride, total cholesterol, LDL-C, HDL-C, malondialdehyde (MDA) and total antioxidant capacity (TAC) were done at the beginning and 34 weeks of gestation. Cord blood serum concentrations of 25(OH)D, iPTH, MDA and TAC were assessed at delivery as well. To determine the effects of vitamin D supplementation on metabolic markers 1-factor repeated-measures analysis of variance (ANOVA) was used. Between groups comparisons was done by using Independent-samples Student’s t-test or Mann-Whitney test. P < 0.05 was considered as significant. Results Supplementation with 1000 IU/d and 2000 IU/d vitamin D resulted in significant changes in vitamin D status over pregnancy (24.01 ± 21.7, P < 0.001 in 1000 IU/d group and 46.7 ± 30.6 nmol/L, P < 0.001 in 2000 IU/d group). Daily intake of 2000 compared with 1000 IU/d tended to increase the serum concentration of HDL-C (10 ± 8.37, P < 0.001 in 1000 IU/d group and 9.52 ± 11.39 mg/dL, P < 0.001 in 2000 IU/d group). A significant decrement in serum concentration of iPTH observed in both groups (− 4.18 ± 7.5, P = 0.002 in 1000 IU/d group and − 8.36 ± 14.17, P = 0.002 in 2000 IU/d group). Conclusions Supplementation with 2000 IU/d vitamin D as compared with 1000 IU/d, is more effective in promoting vitamin D status and HDL-C serum concentration and in decreasing iPTH over pregnancy. Trial registration This trial is registered at clinicaltrials.gov (NCT03308487). Registered 12 October 2017 ‘retrospectively registered’.

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Association between Vitamin D Status, Oxidative Stress Biomarkers and Viral Load in Human Immunodeficiency Virus Type 1 Infection

Current HIV Research ◽

10.2174/1570162x15666171005170227 ◽

2017 ◽

Vol 15 (5) ◽

Cited By ~ 4

Author(s):

Tamires Flauzino ◽

Andrea N.C. Simao ◽

Elaine R.D. de Almeida ◽

Helena K. Morimoto ◽

Sayonara R. Oliveira ◽

...

Keyword(s):

Oxidative Stress ◽

Vitamin D ◽

Human Immunodeficiency Virus ◽

Viral Load ◽

Human Immunodeficiency Virus Type ◽

Vitamin D Status ◽

Oxidative Stress Biomarkers ◽

Stress Biomarkers ◽

Immunodeficiency Virus

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Assessment of physical growth, some oxidative stress biomarkers and vitamin D status in children with Familial Mediterranean Fever

Meta Gene ◽

10.1016/j.mgene.2018.06.010 ◽

2018 ◽

Vol 17 ◽

pp. 212-215 ◽

Cited By ~ 1

Author(s):

Moushira Zaki ◽

Hala T. El-Bassyouni ◽

Hanaa Reyad ◽

Walaa Yousef ◽

Eman Youness ◽

...

Keyword(s):

Oxidative Stress ◽

Vitamin D ◽

Familial Mediterranean Fever ◽

Physical Growth ◽

Vitamin D Status ◽

Oxidative Stress Biomarkers ◽

Stress Biomarkers ◽

Mediterranean Fever

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Vitamin D deficiency, oxidative stress and antioxidant status: only weak association seen in the absence of advanced age, obesity or pre-existing disease

British Journal Of Nutrition ◽

10.1017/s000711451700188x ◽

2017 ◽

Vol 118 (1) ◽

pp. 11-16 ◽

Cited By ~ 15

Author(s):

Erica W. Wang ◽

Parco M. Siu ◽

Marco Y. Pang ◽

Jean Woo ◽

Andrew R. Collins ◽

...

Keyword(s):

Oxidative Stress ◽

Vitamin D ◽

Vitamin D Deficiency ◽

Antioxidant Status ◽

Total Antioxidant Status ◽

Advanced Age ◽

Vitamin D Status ◽

Oxidative Stress Biomarkers ◽

Antioxidant Power ◽

And Oxidative Stress

AbstractVitamin D deficiency (plasma 25-hydroxycholecalciferol (25(OH)D)<50 nmol/l) is highly prevalent, increases risk of non-communicable diseases (NCD) and associates with increased oxidative stress in obese subjects, the elderly and patients suffering from NCD. If confirmed as an independent driver of oxidative stress, nutritional and other public health strategies to improve vitamin D status would be strongly supported. We investigated vitamin D/oxidative stress links without the confounding effects of advanced age, obesity, smoking or pre-existing disease. Plasma 25(OH)D and biomarkers of oxidative stress and antioxidant status (plasma allantoin, oxidised LDL, ferric reducing antioxidant power (FRAP), ascorbic acid, urine 8-oxo-7,8-dihydro-2'-deoxyguanosine) were measured in fasting samples from 196 consenting, healthy adults aged 18–26 years. Correlation between 25(OH)D and each biomarker as well as biomarker differences across 25(OH)D quartiles and groups (<25/25–49/≥50 nmol/l) were investigated. Median 25(OH)D was 40 nmol/l; >70 % of participants were vitamin D deficient. No significant correlations and no biomarker differences across 25(OH)D quartiles or groups were seen except for total antioxidant status. A weak direct association (r0·252,P<0·05) was observed between 25(OH)D and FRAP, and those in the lowest 25(OH)D quartile and group had significantly lower FRAP values. Results did not reveal a clear link between vitamin D status and oxidative stress biomarkers in the absence of advanced age, obesity and disease, though some evidence of depleted antioxidant status in those with vitamin D deficiency was seen. Poor antioxidant status may pre-date increased oxidative stress. Study of effects of correction of deficiency on antioxidant status and oxidative stress in vitamin D-deficient but otherwise healthy subjects is needed.

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The Effects of Vitamin D-K-Calcium Co-Supplementation on Endocrine, Inflammation, and Oxidative Stress Biomarkers in Vitamin D-Deficient Women with Polycystic Ovary Syndrome: A Randomized, Double-Blind, Placebo-Controlled Trial

Hormone and Metabolic Research ◽

10.1055/s-0042-104060 ◽

2016 ◽

Vol 48 (07) ◽

pp. 446-451 ◽

Cited By ~ 14

Author(s):

M. Razavi ◽

M. Jamilian ◽

M. Karamali ◽

F. Bahmani ◽

E. Aghadavod ◽

...

Keyword(s):

Oxidative Stress ◽

Vitamin D ◽

Polycystic Ovary ◽

Controlled Trial ◽

Oxidative Stress Biomarkers ◽

Ovary Syndrome ◽

Double Blind ◽

Double Blind Placebo ◽

Stress Biomarkers ◽

And Oxidative Stress

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Comparisons of the oxidative stress biomarkers levels in gestational diabetes mellitus (GDM) and non-GDM among Thai population: cohort study

Endocrine Connections ◽

10.1530/ec-18-0093 ◽

2018 ◽

Vol 7 (5) ◽

pp. 681-687 ◽

Cited By ~ 16

Author(s):

Hathairat Rueangdetnarong ◽

Rattanaporn Sekararithi ◽

Thidarat Jaiwongkam ◽

Sirinart Kumfu ◽

Nipon Chattipakorn ◽

...

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Glycemic Control ◽

Cord Blood ◽

Inflammatory Marker ◽

Oxidative Stress Biomarkers ◽

Good Glycemic Control ◽

Stress Biomarkers ◽

Tnf Α ◽

Maternal And Neonatal Outcomes

Objective The primary objective of this study was to compare the levels of oxidative stress biomarkers between pregnancies with gestational diabetes mellitus (GDM) and normoglycemic pregnancies. Materials and methods A prospective study was conducted on pregnant women at average risk for GDM. The participants were screened for GDM with glucose challenge test and confirmed by 100 g, 3-h oral glucose tolerance test and categorized into the control (non-GDM) and GDM groups. Maternal blood was collected from all participants at gestational age (GA) 24–28 weeks and early labor and fetal cord blood was collected for measurements of 8 Isoprostane (8Isop) (oxidative stress marker), TNF-α (inflammatory marker) and IL-10 (anti-inflammatory marker) and were followed up for maternal and neonatal outcomes. Result A total of 62 women, 30 in GDM and 32 in control group, met the inclusion criteria. At 24–28 weeks of gestation, maternal serum 8Isop and TNF-α levels were significantly higher in GDM group (P = 0.032 and P = 0.047), in spite of good glycemic control. At early labor, maternal 8Isop levels were significantly higher in GDM (P = 0.001). The biomarkers in the cord blood as well as maternal and neonatal outcomes in both groups were not significantly different. Conclusion GDM is significantly associated with inflammatory process when compared to normal pregnancy, as indicated by higher oxidative stress and apoptosis markers. However, such levels were not correlated with the pregnancy outcomes. An increase in oxidative stress could not be prevented by good glycemic control. Cord blood biomarker levels in pregnancy with GDM were not changed, suggesting that the placenta could be the barrier for the oxidative stress and cytokines.

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