Prevalencia de sensibilización a cucaracha (Periplaneta americana y Blatella germánica). Experiencia del Servicio de alergia e inmunología clínica del Hospital General de México.

María de Lourdes Mendoza-Gertrudis; Alejandro Rosas-Alvarado; Aída Andrea Velasco-Medina; Héctor Alberto Cuevas-Mora; Antonio Albarrán-Godínez; Margaret Gisette Moya-Almonte; Carla Marcela Vallejos-Pereira; Guillermo Velázquez Sámano

doi:10.29262/ram.v67i3.773

Prevalencia de sensibilización a cucaracha (Periplaneta americana y Blatella germánica). Experiencia del Servicio de alergia e inmunología clínica del Hospital General de México.

Revista Alergia México ◽

10.29262/ram.v67i3.773 ◽

2021 ◽

Vol 67 (3) ◽

Author(s):

María de Lourdes Mendoza-Gertrudis ◽

Alejandro Rosas-Alvarado ◽

Aída Andrea Velasco-Medina ◽

Héctor Alberto Cuevas-Mora ◽

Antonio Albarrán-Godínez ◽

...

Keyword(s):

Periplaneta Americana ◽

Blatella Germanica ◽

Hospital General

Antecedentes: Las pruebas cutáneas por técnica de punción son herramientas importantes para el diagnóstico de la alergia respiratoria. Se han identificado los antígenos de las cucarachas como responsables del desarrollo de rinitis, asma y otras enfermedades alérgicas. Objetivo: Demostrar la participación del antígeno de cucaracha en enfermedades alérgicas. Métodos: Se llevó a cabo un estudio retrolectivo y transversal, para el cual se revisaron historias clínicas y los antecedentes de atopia; se recolectaron los resultados de biometría hemática, citología nasal, IgE total y estudio coproparasitoscópico seriado. Previo consentimiento informado se aplicaron pruebas cutáneas en pacientes de tres a 74 años. Resultados: Se estudiaron 1837 pacientes, la prevalencia de reactividad cutánea al antígeno de cucaracha fue de 17.90 %; 56 % de los pacientes tenía diagnóstico de rinitis alérgica y solo 6 %, de asma y rinitis. Conclusión: Se debe considerar la aplicación del antígeno de cucaracha en las pruebas cutáneas en la práctica del alergólogo.

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SENSIBILIZAÇÃO DA BLOMIA TROPICALIS EM PACIENTES ASMÁSTICOS EM SÃO LUÍS E REGIÕES METROPOLITANA

Revista Ceuma Perspectiva ◽

10.24863/rccp.v30i2.149 ◽

2019 ◽

Vol 30 (2) ◽

pp. 28

Author(s):

Poliana Oliveira Lemos de Brito ◽

Thalita Diniz Duarte ◽

João Lucas do Carmo Lima ◽

Caroline Zilma Kalil de Paula Costa Pereira ◽

Camila Rodrigues Azevedo ◽

...

Keyword(s):

Periplaneta Americana ◽

Dermatophagoides Farinae ◽

Blomia Tropicalis ◽

Blatella Germanica

As doenças alérgicas podem causar comprometimento da qualidade de vida das crianças, no qual está exposto a uma série de alérgenos, que podem gerar uma resposta do sistema imunológico como reações de hipersensibilidade. O objetivo da pesquisa foi avaliar o perfil sócio-econômico e ambiental de crianças e adolescentes sensibilizados a Blomia tropicalis (Blo t) em São Luís e região metropolitana. Aprovada pelo comitê de ética e pesquisa, com o parecer de numero: 58737916.30000.5084. Foi realizado um estudo epidemiológico por meio de análise de prontuários em pacientes do Hospital da Criança de São Luís-MA, onde foram submetidos ao teste cutâneo para o alérgeno Blo t e aplicado o questionário ISSAC e complementar. Teste estatístico utilizado foi o Qi-quadrado- Fisher. A população estudada foi constituída por 87 crianças e adolescentes, entre idade de 1 a 17 anos. Na caracterização clínica destes pacientes foi observado uma associação entre a sensibilização o Blo t e a sensibilização ao Dermatophagoides pteronissynus, Dermatophagoides farinae, Periplaneta americana, Blatella germânica e ao cachorro. Observou-se também uma associação com baixa renda familiar, asma e eczema atópico. Quanto a caracterização ambiental observou-se uma correlação entre manchas na parede (mofo) e forro no quarto. Concluímos que a sensibilização o Blo t é um fator de risco para desenvolvimento de asma e eczema e esta relacionado com vários alérgenos, a baixa renda familiar e que o controle ambiental para blo t é essencial para diminuir a prevalência da asma e do eczema e para melhorar a qualidade vida.PALAVRAS-CHAVE: asma; alérgeno; hipersensibilidade

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Deltamethrin contact exposure mediated toxicity and histopathological aberrations in tissue systems of cockroach species Periplaneta americana and Blatella germanica

10.1101/2021.12.06.471460 ◽

2021 ◽

Author(s):

Sunil Dhiman ◽

Kavita Yadav ◽

BN Acharya ◽

DP Nagar ◽

Rama Rao Ghorpade

Keyword(s):

Periplaneta Americana ◽

Microscopic Analysis ◽

Cellular Level ◽

Food Material ◽

Test Species ◽

Blatella Germanica ◽

Short Term Exposure ◽

Contact Exposure ◽

Delayed Mortality ◽

Tissue Systems

AbstractCockroach species Periplaneta americana and Blatella germanica potentially survive in locations close to human activity. Besides spoiling food material, cockroaches also transfer pathogens of different diseases among human. Since the insecticides have been used extensively to control cockroaches, information on their insecticide susceptibility and toxicity at cellular level may be crucial. In the study, deltamethrin toxicity as well as the deltamethrin-mediated cytomorphological changes in brain, ovary and midgut of the two important cockroach species has been assessed. Different concentrations [0.00025% (0.0025mg/ml), 0.0025% (0.025mg/ml), 0.025 (0.25mg/ml), 0.25% (2.5mg/ml), 0.5% (5mg/ml), 1% (10mg/ml)] of deltamethrin in acetone were used to expose test species in WHO bottle assay. Knockdown was recorded after 5 min interval while delayed mortality was observed after 24 hr. Brain, ovary and gut were dissected post 1 hr exposure and 24 hr holding (for 0.25%, 0.5% and 1% concentration), and tissues were processed for microscopic analysis. Deltamethrin exposed cockroaches and dissected tissues were used to estimate deltamethrin using HPLC. At 0.00025% (lowest concentration), the percentage knock-down observed was 66.7% for P. americana and 80% B. germanica respectively (R2= 0.78; p=0.0001) in 1 hr. KDT50 value was found to be 8.7 min (95% CI: 7.3-10.2), while KDT99 was 20.7 min (95% CI: 16.0-35.7) in P. americana at 1% concentration. Whereas, the KDT50 and KDT99 values for B. germanica were 7.4 min (95% CI: 5.4-9.1) and 27.4 min (95% CI: 18.2-80.0) at similar concentration. LD50 and LD95 values (for 60 min standard exposure) were 0.0006 % (95% CI: 0.00-0.001) and 0.034% (95% CI: 0.013-0.49) respectively for P. americana, while these values were 0.0005 (95% CI: 0.00-0.001) and 0.04 (95% CI: 0.01-0.23) for B. germanica. Exposure to 1% deltamethrin induced considerable toxic effect in the epithelial cells in the midgut. HPLC estimated 0.21±0.05 mg (95% CI - 0.18-0.25; CoV 23.9%) deltamethrin in P. americana post 1% exposure. Even short term exposure of low concentration of synthetic pyrethroid deltamethrin displayed immediate knockdown and delayed mortality in both the test species. Considerable histological damage was observed in both the insects at 1% exposure. In India, resistance to deltamethrin may have been reported among different insects due its extensive use, however the formulations such as insecticide paints, attractant baits etc. developed using deltamethrin as active ingredient could be useful in cockroach control operations.

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Efficacy Test on Some Entomopathogenic Bacterial Isolates of the Periplaneta americana and Blatella germanica Cockroaches (Orthoptera) at Laboratory Scale

Biosaintifika Journal of Biology & Biology Education ◽

10.15294/biosaintifika.v10i2.12934 ◽

2018 ◽

Vol 10 (2) ◽

pp. 306-312

Author(s):

Deni Zulfiana ◽

Monaliza Sekar Rini ◽

Bramantyo Wikantyoso ◽

Ni Putu Ratna Ayu Krishanti

Keyword(s):

Periplaneta Americana ◽

Bacterial Isolate ◽

Biological Control Agent ◽

Control Agent ◽

Morphological Observation ◽

Bacterial Isolates ◽

Blatella Germanica ◽

Disease Vector ◽

Negative Impacts ◽

Spraying Method

Cockroach is one of insects which plays a role as a disease vector, causing negative impacts to human health. Controlling cockroach by using excessive insecticide adds residue to the environment and strengthens its resistance toward insecticides. The objective of this research was to implement and evaluate the alternative use of biological control agent, such as entomopathogenic bacteria against two types of cockroach. In this study, three entomopathogenic bacterial isolates derived from dead Spodoptera litura pupa and Bacillus thuringiensis were employed. The test was performed by administering spraying and baiting methods at cell concentration of 108 CFU/ml. The results showed that three entomopathogenic bacterial isolates used in this study affected cockroach mortality. The result of morphological observation and Postulate Koch test showed that cockroach mortality was caused by the bacterial isolates. The spraying method on BLSP4 bacterial isolate contributed to the highest mortality rate by 80% to the Blatella germanica. Meanwhile, in the baiting method, the isolate of B. thuringiensis caused the highest mortality to the Periplaneta americana by 10%. Based on the mortality period, it can be recognized that BLSP4 treatment with spraying method affected the mortality of B. germanica effectively within 2 hours 30 minutes 46 seconds. An important finding in this study was that BLSP4 bacteria has been known potential for controlling cockroach using spraying method. The result of this study provides insights that BLSP4 bacteria can be used as a new alternative for controlling cockroach and generally in pest management.

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Frecuencia de reactividad cutánea hacia antígenos inhalables en pacientes con cuadro clínico de enfermedad alérgica

Revista Alergia México ◽

10.29262/ram.v64i1.185 ◽

2017 ◽

Vol 64 (1) ◽

pp. 7

Author(s):

Isabel Cristina Rojas-Méndez ◽

Oswaldo Arana-Muñoz ◽

Aída Inés López-García ◽

Daniela Rivero-Yeverino ◽

Chrystopherson Gengyny Caballero-López ◽

...

Keyword(s):

Lolium Perenne ◽

Periplaneta Americana ◽

Cynodon Dactylon ◽

Dermatophagoides Pteronyssinus ◽

Dermatophagoides Farinae ◽

Blatella Germanica ◽

Schinus Molle

Antecedentes: El diagnóstico de las enfermedades alérgicas debe basarse en la historia clínica alergológica adecuada y en una prueba inmunológica de sensibilización; la de mayor sensibilidad y especificidad es la prueba cutánea por punción.Objetivo: Determinar la frecuencia de la reactividad cutánea hacia aeroalérgenos, por grupos etarios, en pacientes del Servicio de Alergia e Inmunología Clínica del Hospital Universitario de Puebla, México.Métodos: Se realizó estudio transversal que incluyó a pacientes de 2 a 64 años de edad, con síntomas sugestivos de enfermedad alérgica, en quienes se realizaron pruebas cutáneas con aeroalérgenos; los criterios diagnósticos fueron los de las guías internacionales. Se calcularon frecuencias, porcentajes y medidas de dispersión.Resultados: De 173 pacientes, 63 % fue del sexo femenino. La edad media fue de 22.3 años. La frecuencia de la reactividad cutánea para Quercus sp. fue 12.72 %, Periplaneta americana 9.83 %, Dermatophagoides farinae 9.25 %, Cynodon dactylon 8.09 %, Blatella germanica 8.09 %, Holcus halepensis 6.94 %, Dermatophagoides pteronyssinus 6.36 %, Schinus molle 5.78 %, Fraxinus uhdei 5.20 %, Lolium perenne 5.20 %, Ambrosia eliator 5.20 % y Artemisa tridentata 4.62 %.Conclusión: Los Dermatophagoides son los aeroalérgenos más identificados, pero en el presente estudio fue más común un polen, probablemente debido a factores geográficos-medioambientales, aunque no fue así en el análisis por grupos etarios.

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Baratas (Insecta, Blattaria) sinantrópicas na cidade de Manaus, Amazonas, Brasil

Acta Amazonica ◽

10.1590/s0044-59672008000100020 ◽

2008 ◽

Vol 38 (1) ◽

pp. 173-178 ◽

Cited By ~ 1

Author(s):

José Albertino Rafael ◽

Neliton Marques da Silva ◽

Racy Manuel Najar Sarmento Dias

Keyword(s):

Periplaneta Americana ◽

Supella Longipalpa ◽

Blatella Germanica

A coleta de baratas na cidade de Manaus resultou em seis espécies associadas às habitações, estabelecimentos comerciais e educacionais, sendo quatro predominantemente dentro das habitações, Blatella germanica (Linnaeus, 1758), Supella longipalpa (Fabricius, 1798), Periplaneta americana (Linnaeus, 1758), P. australasiae (Fabricius, 1775) e duas fora das habitações, Pycnoscelus surinamensis (Linnaeus, 1758) e Blaberus parabolicus Walker, 1868. P. americana foi comum tanto interna como externamente às instalações urbanas; P. australasiae foi predominante em barcos; P. surinamensis e B. parabolicus foram invasoras ocasionais de residências na estação chuvosa. São apresentadas fotos coloridas, em tamanho natural, para reconhecimento das espécies.

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The Temperature and Humidity Relations of the Cockroach

Journal of Experimental Biology ◽

10.1242/jeb.12.2.185 ◽

1935 ◽

Vol 12 (2) ◽

pp. 185-190 ◽

Cited By ~ 1

Author(s):

D. L. GUNN

Keyword(s):

Periplaneta Americana ◽

Unit Surface Area ◽

Ecological Significance ◽

Preferred Temperature ◽

Previous Conclusion ◽

Blatella Germanica ◽

Dry Air ◽

Blatta Orientalis ◽

Upper Limit ◽

Unit Surface

1. The rates of desiccation of three species of cockroaches (Periplaneta americana, Blatta orientalis and Blatella germanica) in dry air at various temperatures have been compared (Fig. 1). There are clear specific differences. 2. The rates of respiration of the three species are proportional to the weight to the power of 0 75-0 8. 3. In all three species, at 30° C. in dry air the animal loses about 6 mg. of water by evaporation for every 1 mg. of oxygen consumed. The smallest species (Blatella germanica), with the thinnest cuticle, loses least rapidly per unit surface area. These facts are consistent with the previous conclusion that the water passes through both the spiracles and the cuticle. 4. The upper limit of preferred temperature of Periplaneta americana and Blatella germanica is 33° C, while for Blatta orientalis it is 29° C. The lower limit is not so sharply defined and further work is required before it can be regarded as significant. 5. Certain suggestions are made concerning the ecological significance of the observations described.

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Investigation of the Synthesis of Verbenyl Compounds, Effective Attractants of Cockroach Species, by Acyloxylation of α-Pinene

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19921719 ◽

1992 ◽

Vol 57 (8) ◽

pp. 1719-1730 ◽

Cited By ~ 3

Author(s):

Peter Vinczer ◽

Mária Kajtár-Peredy ◽

Zoltan Juvancz ◽

Lajos Novak ◽

Csaba Szántay

Keyword(s):

Acidic Medium ◽

Large Scale ◽

Periplaneta Americana ◽

Neutral Medium ◽

Blatella Germanica ◽

Insect Attractants ◽

Large Scale Preparation ◽

Scale Preparation ◽

Cyclobutane Ring

The synthesis of verbenyl acetate (Ia) and propionate (Ib) was investigated by acyloxylation of α-pinene with lead(IV) acetate (LTA), lead(IV) propionate (LTP), mercury(II) acetate (MA) and iodosobenzene diacetate (IBDA) in neutral and acidic medium. The neutral medium is better for the formation of Ia and Ib, because the acidic medium helps the opening of cyclobutane ring of α-pinene. These methods can be used for large scale preparation of insect attractants to cockroach species (Blatella germanica, Blatella orientalis and Periplaneta americana).

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