Comparison of Chemical Pretreatment for Recovery of Fermentable Sugars and Enzymatic Saccharification

2017 ◽  
Vol 104 (7-9) ◽  
pp. 273 ◽  
Author(s):  
Maheshwari Packiam ◽  
Karthikeyan Subburamu ◽  
Ramesh Desikan ◽  
Sivakumar Uthandi ◽  
Marimuthu Subramanian ◽  
...  
Keyword(s):  
Enzymatic Saccharification ◽  
Fermentable Sugars ◽  
Chemical Pretreatment

scholarly journals Valorization of sugarcane bagasse by chemical pretreatment and enzyme mediated deconstruction

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Vihang S. Thite ◽  
Anuradha S. Nerurkar
Keyword(s):  
Cell Wall ◽  
Sugarcane Bagasse ◽  
Structural Changes ◽  
Enzymatic Saccharification ◽  
Dry Weight ◽  
Gravimetric Analysis ◽  
Chemical Pretreatment ◽  
Cell Wall Degrading Enzymes ◽  
First Time ◽  
Soluble Components

Abstract After chemical pretreatment, improved amenability of agrowaste biomass for enzymatic saccharification needs an understanding of the effect exerted by pretreatments on biomass for enzymatic deconstruction. In present studies, NaOH, NH4OH and H2SO4 pretreatments effectively changed visible morphology imparting distinct fibrous appearance to sugarcane bagasse (SCB). Filtrate analysis after NaOH, NH4OH and H2SO4 pretreatments yielded release of soluble reducing sugars (SRS) in range of ~0.17–0.44%, ~0.38–0.75% and ~2.9–8.4% respectively. Gravimetric analysis of pretreated SCB (PSCB) biomass also revealed dry weight loss in range of ~25.8–44.8%, ~11.1–16.0% and ~28.3–38.0% by the three pretreatments in the same order. Release of soluble components other than SRS, majorly reported to be soluble lignins, were observed highest for NaOH followed by H2SO4 and NH4OH pretreatments. Decrease or absence of peaks attributed to lignin and loosened fibrous appearance of biomass during FTIR and SEM studies respectively further corroborated with our observations of lignin removal. Application of commercial cellulase increased raw SCB saccharification from 1.93% to 38.84%, 25.56% and 9.61% after NaOH, H2SO4 and NH4OH pretreatments. Structural changes brought by cell wall degrading enzymes were first time shown visually confirming the cell wall disintegration under brightfield, darkfield and fluorescence microscopy. The microscopic evidence and saccharification results proved that the chemical treatment valorized the SCB by making it amenable for enzymatic saccharification.

Effective Extraction of Lignin from Elephant Grass Using Imidazole and Its Effect on Enzymatic Saccharification To Produce Fermentable Sugars

2017 ◽  
Vol 56 (17) ◽  
pp. 5138-5145 ◽  
Author(s):  
Andréia Toscan ◽  
Ana Rita C. Morais ◽  
Susana M. Paixão ◽  
Luís Alves ◽  
Jürgen Andreaus ◽  
...  
Keyword(s):  
Enzymatic Saccharification ◽  
Fermentable Sugars ◽  
Elephant Grass

Bioethanol Production from Rice Straw in the MAFF Research Project, Japan

2009 ◽  
Vol 1219 ◽  
Author(s):  
Ken Tokuyasu
Keyword(s):  
Heat Treatment ◽  
Saccharomyces Cerevisiae ◽  
Rice Straw ◽  
Enzymatic Saccharification ◽  
Bioethanol Production ◽  
Chemical Pretreatment ◽  
Simple Method ◽  
Herbaceous Biomass ◽  
Free Glucose ◽  
Simultaneous Saccharification

AbstractRice straw is among the most abundant herbaceous biomass, and regarded as the central feedstock for bioethanol production in Japan. We found that significant amounts of soft carbohydrates (SCs), defined as carbohydrates readily recoverable by mere extraction from the biomass or brief enzymatic saccharification, exist in rice straw in the form of free glucose, free fructose, sucrose, starch, and β-1,3-1,4-glucan. Based on the finding, we proposed a simple method for bioethanol production from rice straw samples with SCs, by a heat treatment for sterilization and starch gelatinization, followed by simultaneous saccharification/fermentation with Saccharomyces cerevisiae. This method would offer an efficient process for bioethanol production without the aid of harsh thermo/chemical pretreatment step.

Kinetic Study on the Pretreatment and Enzymatic Saccharification of Rice Hull for the Production of Fermentable Sugars

2010 ◽  
Vol 162 (5) ◽  
pp. 1471-1482 ◽  
Author(s):  
Gong-Yuan Wei ◽  
You-Jung Lee ◽  
Yi-Joon Kim ◽  
Il-Hyuck Jin ◽  
Jai-Heon Lee ◽  
...  
Keyword(s):  
Kinetic Study ◽  
Enzymatic Saccharification ◽  
Fermentable Sugars ◽  
Rice Hull

scholarly journals Study of enzymatic saccharification of Agave leaves biomass to yield fermentable sugars

3 Biotech ◽  
2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Miguel A. Medina-Morales ◽  
Oscar Soto-Cruz ◽  
Juan C. Contreras-Esquivel ◽  
Raúl Rodríguez-Herrera ◽  
Heliodoro De la Garza-Toledo ◽  
...  
Keyword(s):  
Enzymatic Saccharification ◽  
Fermentable Sugars

scholarly journals LPMO AfAA9_B and Cellobiohydrolase AfCel6A from A. fumigatus Bost Enzymatic Saccharification Activity of Cellulase Cocktail

2020 ◽  
Author(s):  
Taisa Magnani Dinamarco ◽  
Aline Vianna Bernardi ◽  
Luis Eduardo Gerolamo ◽  
Paula Fagundes de Gouvêa ◽  
Deborah Kimie Yonamine ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Lignocellulosic Biomass ◽  
Filamentous Fungi ◽  
Biochemical Parameters ◽  
Enzymatic Saccharification ◽  
Fermentable Sugars ◽  
Optimal Temperature ◽  
High Turnover ◽  
Lytic Polysaccharide Monooxygenase ◽  
Polysaccharide Monooxygenase

Cellulose is the most abundant polysaccharide in lignocellulosic biomass, where it is interlinked with lignin and hemicellulose. Bioethanol can be produced from biomass. Because breaking down biomass is difficult, cellulose-active enzymes secreted by filamentous fungi play an important role in degrading recalcitrant lignocellulosic biomass. We characterized a cellobiohydrolase (AfCel6A) and lytic polysaccharide monooxygenase LPMO (AfAA9_B) from A. fumigatus after they were expressed in Pichia pastoris and purified. The biochemical parameters suggested that the enzymes were stable; the optimal temperature was ~60 °C. Further characterization revealed high turnover numbers (kcat of 147.9 s-1 and 0.64 s-1, respectively). Surprisingly, when combined, AfCel6A and AfAA9_B did not act synergistically. Association of AfCel6A and AfAA9_B inhibits the activity of AfCel6A, an outcome that needs to be further investigated. However, addition of AfCel6A or AfAA9_B boosts the enzymatic saccharification activity of a cellulase cocktail and the activity of cellulase Af-EGL7. The supplementation of an enzymatic cocktail with AfCel6A or AfAA9_B boosted the yield of fermentable sugars from complex substrates, especially sugarcane exploded bagasse, by up to 95%. The synergism between the cellulase cocktail and AfAA9_B is enzyme- and substrate-specific, which suggests a specific enzymatic cocktail for each biomass.

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