scholarly journals Role of HRCT Thorax in pre-operative assessment of RT-PCR COVID-19 negative oral cancer patients

2020 ◽  
Vol 4 (3) ◽  
pp. 008-011
Author(s):  
Ganta Roopa ◽  
Kantharia Shehnaz R ◽  
Shah Bhavin Bhupendra ◽  
Vasava Rajinikant Mohan ◽  
Vaishnav Tushar Vishnuprasad ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Cancer Patients ◽  
Public Transportation ◽  
Rt Pcr ◽  
Care Workers ◽  
Number Of Patients ◽  
Oral Cancer Patients ◽  
Complete Disruption ◽  
Operative Assessment

Background: Oral cancer accounts for 30% of all types of cancer in India. Surgery is the mainstay of treatment but due to the recent outbreak of COVID-19, there is a partial or complete disruption of health services in the country. The initial delay in the treatment was due to cancellations of planned surgeries as per government regulations and reduction in public transportation. In the latter half of the initial relaxation of the lockdown, we formulated our institutional protocol for the surgical treatment of oral cancer patients. On admission, all patients were kept in isolated wards followed by RT-PCR (Reverse Transcriptase-Polymerase Chain Reaction) testing on the same day. RT-PCR negative patients are subjected to HRCT Thorax (High-resolution Computer Tomography). This ensured the safety of health care workers, patients, and patient attendees. Keeping this in mind, we did an observational study on the role of HRCT in the pre-operative screening of asymptomatic oral cancer patients. Materials and methods: Retrospective analysis of prospectively collected data of 150 patients was done. HRCT reporting was done by two experienced senior radiologists of the Department of Radiology at our hospital. Results: The number of patients under the CO-RADS 1 category was 121 and CO-RADS 2 category was 29, according to CO-RADS classification. The results of RT-PCR and HRCT were compared and there was a 100% positive correlation between RT-PCR and HRCT Thorax. Conclusion: Our study supported the use of HRCT Thorax as a diagnostic tool in pre-operative screening of oral cancer patients for COVID 19, particularly in RT-PCR negative cases.

Prognostic role of Oct4, CD44 and c-Myc in radio–chemo-resistant oral cancer patients and their tumourigenic potential in immunodeficient mice

2015 ◽  
Vol 20 (1) ◽  
pp. 43-56 ◽  
Author(s):  
Sharada Sawant ◽  
Ravindran Gokulan ◽  
Harsh Dongre ◽  
Milind Vaidya ◽  
Devendra Chaukar ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Cancer Patients ◽  
Prognostic Role ◽  
Immunodeficient Mice ◽  
Oral Cancer Patients

scholarly journals Droplet Digital PCR Analysis of Liquid Biopsy Samples Unveils the Diagnostic Role of hsa-miR-133a-3p and hsa-miR-375-3p in Oral Cancer

Biology ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 379
Author(s):  
Salvatore Crimi ◽  
Luca Falzone ◽  
Giuseppe Gattuso ◽  
Caterina Maria Grillo ◽  
Saverio Candido ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Cancer Patients ◽  
Liquid Biopsy ◽  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Pcr Analysis ◽  
Screening Programs ◽  
Diagnostic Potential ◽  
Oral Cancer Patients

Despite the availability of screening programs, oral cancer deaths are increasing due to the lack of diagnostic biomarkers leading to late diagnosis and a poor prognosis. Therefore, there is an urgent need to discover novel effective biomarkers for this tumor. On these bases, the aim of this study was to validate the diagnostic potential of microRNAs (miRNAs) through the analysis of liquid biopsy samples obtained from ten oral cancer patients and ten healthy controls. The expression of four selected miRNAs was evaluated by using droplet digital PCR (ddPCR) in a pilot cohort of ten oral cancer patients and ten healthy donors. Bioinformatics analyses were performed to assess the functional role of these miRNAs. The expression levels of the predicted down-regulated hsa-miR-133a-3p and hsa-miR-375-3p were significantly reduced in oral cancer patients compared to normal individuals while no significant results were obtained for the up-regulated hsa-miR-503-5p and hsa-miR-196a-5p. ROC analysis confirmed the high sensitivity and specificity of hsa-miR-375-3p and hsa-miR-133a-3p. Therefore, both miRNAs are significantly down-regulated in cancer patients and can be used as biomarkers for the early diagnosis of oral cancer. The analysis of circulating miRNAs in a larger series of patients is mandatory to confirm the results obtained in this pilot study.

Uroporphyrinogen decarboxylase gene expression in oral squamous cell carcinomas.

2013 ◽  
Vol 31 (15_suppl) ◽  
pp. e17002-e17002
Author(s):  
Rajkumar Kottayasamy Seenivasagam ◽  
Arun Kumar Ganesan ◽  
Rajaraman Ramamurthy ◽  
Munirajan Arasambattu Kannan ◽  
Deva Magendhra Rao
Keyword(s):  
Oral Cancer ◽  
Cancer Patients ◽  
Squamous Cell ◽  
Splice Variants ◽  
Squamous Cell Carcinomas ◽  
Uroporphyrinogen Decarboxylase ◽  
Number Of Patients ◽  
Oral Cancer Patients ◽  
The Mean

e17002 Background: Oral cancer is one of the most common cancers in India. Uroporphyrinogen decarboxylase (UROD), a key regulator of heme biosynthesis, has recently been suggested as a novel tumor-selective radiosensitizing target against head and neck cancers. Further, a recent study has suggested that tumors with UROD expression may have poorer outcomes and higher rates of recurrence. We tested the expression of UROD splice variants in oral cancer patients who were treated in our hospital to study its expression and impact on the patients. Methods: The expression of both coding and noncoding splice variants of UROD gene was tested in 42 patients (31 male, 11 female) with oral cavity squamous cell carcinomas treated in 2011-12. Pretreatment tumor biopsies were collected and mRNA was extracted using Quiagen RNeasy kit method. cDNA was synthesized using Superscript III and subjected for UROD expression using specific primers. UROD expression and clinical data of patients were analyzed using IBM SPSS 20 Software. Results: The mean age of the patients was 52 years (range 32-70). Buccal mucosa (n=17) and tongue (n=13) were the most common subsites. Seventy six percent had locally advanced disease (T3/T4 and node-positive) and high-grade (70%) cancers. Chemoradiotherapy (CRT) (n =25) and chemotherapy (CT) (n = 10) was given in 35 patients of which 5 had progressive disease. The mean follow up was 11 months. Thirty seven patients were operated, 5 developed locoregional recurrence and 2 developed spine metastases and died during follow-up. UROD was expressed in 23 (54.8%) patients (coding variant = 52.8%, noncoding = 26%). There was no correlation between UROD expression and age, sex, subsite, stage, grade or node positivity. Patients with UROD expression had a slightly poorer response to CRT/CT (30.4% vs 24.1%; p =NS). Similarly, though they had a slightly higher incidence of recurrence, the difference was not significant. Both patients with spinal recurrence had UROD expression. Conclusions: UROD is highly expressed in oral cancer patients in India. Its proposed radiosensitizing and prognostic role in treatment and outcomes though promising needs further evaluation in a larger number of patients.

Abstract 3700: Role of salivary miRNAs and their target genes in oral cancer patients as a potential diagnostic tool

2020 ◽  
Author(s):  
Muhammad Jawad Khan ◽  
Andleeb Zahra ◽  
Ghulam Rabia ◽  
Syeda Aba Ali ◽  
Shanza Sahar ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Cancer Patients ◽  
Diagnostic Tool ◽  
Target Genes ◽  
Oral Cancer Patients

scholarly journals Role of Monocyte Count and Neutrophil-to-Lymphocyte Ratio in Survival of Oral Cancer Patients

2016 ◽  
Vol 21 (01) ◽  
pp. 21-27 ◽  
Author(s):  
Saurabh Bobdey ◽  
Balasubramaniam Ganesh ◽  
Prabhashankar Mishra ◽  
Aanchal Jain
Keyword(s):  
Oral Cancer ◽  
Cancer Patients ◽  
Neutrophil To Lymphocyte Ratio ◽  
Monocyte Count ◽  
Lymphocyte Ratio ◽  
Oral Cancer Patients

scholarly journals Oral Cancer Surgery and COVID 19 Pandemic: Our Experience

2020 ◽  
Vol 5 (2) ◽  
Author(s):  
Shehnaz Kantharia
Keyword(s):  
Oral Cancer ◽  
Cancer Patients ◽  
Initial Period ◽  
Operative Procedure ◽  
Hospital Staff ◽  
Cancer Surgery ◽  
Rt Pcr ◽  
Oral Cancers ◽  
Emergency Procedures ◽  
Oral Cancer Patients

The COVID 19 pandemic limited the clinical practice of Surgical Oncologists all over the world to performing emergency procedures and surgeries. During this initial period of two to three weeks we realised that further delay in the treatment of curable oral cancers would have serious adverse prognostic implications. So we started the process to formulate our own institutional guidelines to screen and test all preoperative asymptomatic oral cancer patients for COVID 19. All preoperative oral cancer patients were tested for Reverse Transcriptase Polymerase Chain Reaction (RT PCR) and a negative COVID RT PCR report was followed by HRCT Chest. We share our prospectively collected data of Oral Cancer Surgery patients from 14th April to 31st May 2020 with details of sub site, stage, operative procedure and type of reconstruction offered. Our institutional protocol to test all preoperative patients by the highly sensitive HRCT Chest combined with the gold standard RT PCR assay ensured the safety of our patients, attendants and the hospital staff.

The Role of Socioeconomic Status and Literacy in the treatment delay in Oral Cancer patients Visiting Mayo Hospital

2021 ◽  
Vol 15 (8) ◽  
pp. 1785-1787
Author(s):  
Ahmad Liaquat ◽  
Rabia Naseer ◽  
Muhammad Rashid ◽  
Saqib Mehmood Khan ◽  
Ehsan Ul Haq ◽  
...  
Keyword(s):  
Socioeconomic Status ◽  
Oral Cancer ◽  
Cancer Patients ◽  
Treatment Delay ◽  
Stage Iii ◽  
Care Hospital ◽  
Low Socioeconomic ◽  
Oral Cancer Patients ◽  
Late Stages

Background: One of the most important prognostic factors of oral cancer is the stage at the time of diagnosis. Unfortunately, most of the patients present in the late stages (stage III and IV). Aim: To assess the role of socioeconomic status and literacy in the treatment delay in patients of oral cancer. By identifying their role, we can educate the general public and healthcare doctors to detect and treat oral cancer. Method: This was a prospective study conducted in the Mayo hospital, a tertiary care hospital of Pakistan, from June 2018 to June 2020. Results: Out of 120 patients, 84 (70%) were males and 36 (30%) were females. The age range was 14 to 85 years. One hundred and eight patients (90%) presented with the late stages (stage III and IV). These late-stage presenter patients belonged to the low socioeconomic status (88.88%) and 83.33% of them were illiterate (<high school education). Only twelve patients (10%) presented with the early stage at the time of diagnosis. Conclusion: Low socioeconomic and lack of education have a significant association with the late presentation of oral cancer patients in the Pakistani population. Keywords: squamous cell carcinoma, treatment delay, socioeconomic status

Detection of oncometabolite nicotine imine in the nail of oral cancer patients and predicted as an inhibitor of DNMT1

2021 ◽  
Vol 16 ◽  
Author(s):  
Anwesha Deep Dutta ◽  
Ajay Kumar ◽  
Kiran Bharat Lokhande ◽  
Manmohan Mitruka ◽  
K. Venkateswara Swamy ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Oral Cancer ◽  
Cancer Patients ◽  
Dna Methyltransferase ◽  
Biological Fluids ◽  
Md Simulations ◽  
Dynamics Simulation ◽  
Dna Hypomethylation ◽  
Oral Cancer Patients

Background: Nicotine-metabolized product nicotine imine is suggested to play a role in metabolic changes in oral cancer. There is a significant gap in the detection of oncometabolite nicotine imine in biological fluids and nails of oral cancer patients. Oncometabolites are designated as metabolites those are usually elevated in cancer cells over normal cells. Interestingly, a direct or indirect link is missing that establishes a role of nicotine imine in pro-cancer cellular events including global DNA hypomethylation, a potential metabolic-epigenetic axis in oral cancer. Methods: A novel vertical tube gel electrophoresis (VTGE) system assisted purification and liquid chromatography-high resolution mass spectrometry (LC-HRMS) based identification of nicotine imine in the nails of oral cancer patients. Further, nicotine imine was evaluated for its molecular interactions with various methyltransferases including DNA methyltransferase 1 (DNMT1) by molecular docking and molecular dynamics (MD) simulations. Results: Data suggested the presence of nicotine imine in the nails of oral cancer patients. Molecular docking and MD simulations revealed a specific binding affinity by nicotine imine with DNMT1. Binding by nicotine imine is within the CXCC regulatory domain of DNMT1 including key residues as ARG690, PRO574, VAL658, PRO692 and ALA695. Similar binding residues are displayed by DNMT1 inhibitor 5'-Aza-2'-deoxycytidine. Conclusion : Nicotine imine is suggested as a predictive biomarker for oral cancer patients in nails and this finding is a first report. Molecular docking and dynamics simulation propose the role of nicotine imine as an inhibitor of DNMT1. This work supports the involvement of synergistic pro-tumor metabolic-epigenomic axis by nicotine imine that may contribute towards potential mutagenesis of normal squamous epithelium.

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