scholarly journals Flavonoids and Triterpenoids from the Roots of Rosa laevigata

2017 ◽  
Vol 58 (4) ◽  
Author(s):  
Ning Li ◽  
Kaijin Wang ◽  
Shiping Li ◽  
Xiangyu Zhai ◽  
Tianming Wang ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Methyl Ether ◽  
Betulinic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Assay ◽  
Isolation And Identification ◽  
Phytochemical Investigation ◽  
Rosa Laevigata

<p>Phytochemical investigation on the root of <em>R. laevigata</em> led the isolation and identification of two new flavonoids, (+)-catechin-8-acetic acid (<strong>1</strong>) and guibourtacacidine 4-methyl ether (<strong>2</strong>), one known flavonoid, guibourtacacidine (<strong>3</strong>), along with seven known triterpenoids, euscaphic acid (<strong>4</strong>), kajiichigoside F1 (<strong>5</strong>), nigaichigoside F2 (<strong>6</strong>), rubuside J (<strong>7</strong>), tomentic acid (<strong>8</strong>), rosamutin (<strong>9</strong>) and betulinic acid (<strong>10</strong>). Their structures were established on the basis of spectroscopic evidence and comparisons with literature data. Compounds <strong>1</strong>, <strong>2</strong> and <strong>3</strong> showed considerable radical scavenging activity in the 1, 1-diphenyl-2-picrylhydrazyl (DPPH) assay.<strong></strong></p>

scholarly journals 2,4-Furfurylidene-D-sorbitol and its tetra-methyl ether: synthesis, conformational studies, and radical scavenging activity

ARKIVOC ◽  
2016 ◽  
Vol 2016 (5) ◽  
pp. 50-68 ◽  
Author(s):  
Pierangela Ciuffreda ◽  
Andrea Brizzolari ◽  
Silvana Casati ◽  
Ivano Eberini ◽  
Luca Palazzolo ◽  
...  
Keyword(s):  
Methyl Ether ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Conformational Studies ◽  
Ether Synthesis

Non-Phenolic Antioxidant Compounds from Buddleja asiatica

2008 ◽  
Vol 63 (7-8) ◽  
pp. 483-491 ◽  
Author(s):  
Mortada M. El-Sayed ◽  
El-Sayed S. Abdel-Hameed ◽  
Wafaa S. Ahmed ◽  
Eman A. El-Wakil
Keyword(s):  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Compounds ◽  
Scavenging Activity ◽  
Chemical Analyses ◽  
Dpph Assay ◽  
Antioxidant Agents ◽  
Total Antioxidant

The methanol extract of the leaves of Buddleja asiatica Lour. (Loganiaceae) showed antioxidant activity toward the well known in vitro antioxidant tests such as total antioxidant capacity by the phosphomolybdenum method, free radical scavenging activity by the 1,1- diphenyl-2-picrylhydrazyl scavenging assay (DPPH assay) and hydrogen peroxide scavenging methods. Due to the high scavenging activity of the n-butanol successive fraction toward DPPH and H2O2 (SC50 = 11.99 and 18.54 μg/ml, respectively), this extract was subjected to chromatographic separation and isolation. Four non-phenolic compounds were isolated and identified on the basis of spectroscopic and chemical analyses: 1-O-ß-D-glucopyranosyl- 2-methoxy-3-(2-hydroxy-triaconta-3,12-dienoate)-glycerol (1), 3-O-[α-l-rhamnopyranosyl- (1→4)-ß-d-glucopyranosyl-(1→3)]-[ß-d-glucopyranosyl-(1→2)]-ß-d-fucopyranosyl-olean- 11,13(18)-diene-3ß,23,28-triol (2), 3-O-[α-l-rhamnopyranosyl-(1→4)-ß-d-glucopyranosyl- (1→4)-ß-d-glucopyranosyl-(1→3)]-ß-d-fucopyranosyl-olean-11,13(18)-diene-3ß,23,28-triol (3), and 3-O-[α-l-rhamnopyranosyl-(1→4)-ß-d-glucopyranosyl-(1→3)]-[ß-d-xylopyranosyl- (1→2)]-ß-d-glucuronopyranosyl-acid-olean-11,13(18)-diene-3ß,23,28-triol (4). The four compounds were evaluated as antioxidant agents using the three antioxidant bioassay tests.

Isolation and identification of antioxidant peptides derived from whey protein enzymatic hydrolysate by consecutive chromatography and Q-TOF MS

2013 ◽  
Vol 80 (3) ◽  
pp. 367-373 ◽  
Author(s):  
Qiu-Xiang Zhang ◽  
Hui Wu ◽  
Yu-Fang Ling ◽  
Rong-Rong Lu
Keyword(s):  
Whey Protein ◽  
Superoxide Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Gel Filtration ◽  
Scavenging Activity ◽  
Antioxidant Peptides ◽  
Isolation And Identification ◽  
Tof Ms

To isolate and identify antioxidant peptides from enzymatically hydrolysed whey protein, whey protein isolate was hydrolysed by different protease (trypsin, pepsin, alcalase 2·4L, promatex, flavourzyme, protease N). The hydrolysate generated by alcalase 2·4L had the highest antioxidant activities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, superoxide radicals and in a linoleic acid peroxidation system induced by Fe2+. The IC50 values of DPPH and superoxide radical scavenging activities of the hydrolysate decreased significantly (6·89 and 38·88%, respectively) after treatment with macroporous adsorption resin. Seven different peptides showing strong antioxidant activities were isolated from the hydrolysate using consecutive chromatographic methods including gel filtration chromatography and high-performance liquid chromatography. The molecular mass and amino acids sequences of the purified peptides were determined using a Quadrupole time-of-flight mass spectrometer (Q-TOF MS). One of the antioxidative peptides, Trp–Tyr–Ser–Leu, displayed the highest DPPH radical scavenging activity (IC50=273·63 μm) and superoxide radical scavenging activity (IC50=558·42 μm). These results suggest that hydrolysates from whey proteins are good potential source of natural antioxidants.

scholarly journals Antioxidant Activity of Moringa oleifera Extracts

2018 ◽  
Vol 16 (3) ◽  
pp. 297 ◽  
Author(s):  
Wiwit Denny Fitriana ◽  
Taslim Ersam ◽  
Kuniyoshi Shimizu ◽  
Sri Fatmawati
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Moringa Oleifera ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Dpph Assay ◽  
Abts Assay

Moringa oleifera have been evaluated for its antioxidant activity. M. oleifera leaves were extracted with methanol, ethyl acetate, dichloromethane and n-hexane. The antioxidant activity of extracts were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity assay and an improved 2,2’-azino-bis-[3-ethylbenzothiazoline sulphonate] (ABTS) radical cation decolorization assay in vitro. Trolox was used as standard with IC50 5.89 μg/mL in DPPH assay and 3.06 μg/mL in ABTS assay. The methanol extract showed the highest free radical scavenging activity with IC50 value of 49.30 μg/mL in DPPH assay and 11.73 μg/mL in ABTS assay. This study provided that M. oleifera leaves possess antioxidant.

scholarly journals Preliminary Phytochemical Screening and In vitro Antioxidant Activity of Ethiopian Indigenous Medicinal Plants, Ocimum lamiifolium Hochst. ex Benth and Ocimum basilicum L.

2016 ◽  
Vol 8 (01) ◽  
Author(s):  
Suresh Kumar P. Nair ◽  
Kumar Ganesan ◽  
Henok Azalewor ◽  
Neethu Letha ◽  
Sharmila Gani
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Free Radical Scavenging ◽  
Ocimum Basilicum ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
Phytochemical Investigation

Antioxidants are the chemical substances which prevent the free radicals damage in the body. Numerous researches are going globally focussed on investigating natural antioxidants of plants origins. The aims of the present study were to evaluate preliminary phytochemical investigation and in vitro antioxidant activities of Ethiopian indigenous medicinal plants, Ocimum lamiifolium Hochst. ex Benth and Ocimum basilicum L. Aqueous, benzene and hexane crude leaves extracts of O. lamiifolium and O. basilicum were subjected to qualitative phytochemical screening using standard procedures. In addition, we investigated the antioxidant potential of crude aqueous leaves extract of O. lamiifolium and O. basilicum using tests involving inhibition of superoxide anions, DPPH, H2O2, NO and ABTS. Preliminary phytochemical investigation for benzene, hexane and aqueous extracts found alkaloids, sterols, carbohydrate and glycosides, tannins and flavonoids. The fraction inhibition of lipid peroxide at the first stage of oxidation illustrated antioxidant activity of O. lamiifolium and O. basilicum as 90% and 88% compared to those of gallic acid (97%) and BHT (84%) respectively. Also, the aqueous leaves extract of O. lamiifolium and O. basilicum exhibited significant DPPH free radical scavenging activity, nitric acid free radical scavenging activity assay, superoxide anion scavenging activity, ABTS scavenging activity and hydrogen peroxide free radical scavenging assay. Our findings provide confirmation that the aqueous leaves extract of O. lamiifolium and O. basilicum are potential source of natural antioxidants, and this warranted its uses in traditional medicine systems.

scholarly journals Chemical Characterization, Mineral Content and Radical Scavenging Activity of Sideritis scardica and S. raeseri from R. Macedonia and R. Albania

2013 ◽  
Vol 8 (5) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Marija Karapandzova ◽  
Bujar Qazimi ◽  
Gjoshe Stefkov ◽  
Katerina Bačeva ◽  
Trajče Stafilov ◽  
...  
Keyword(s):  
Toxic Elements ◽  
Mineral Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Chemical Characterization ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Assay ◽  
Tea Infusions ◽  
Hexane Extracts

Chemical characterization was made of over-ground flowering parts of Sideritis scardica from R. Macedonia and S. raeseri from both R. Macedonia and R. Albania. GC/FID/MS investigation of the n-hexane extracts revealed more than 90 components, dominated by diterpenes and hydrocarbons. The most abundant components were hentriacontane, nonacosane and heptacosane, and two other components both with MW=286, probably diterpenes, which were not fully identified. In addition, the content of total phenols, made by the Folin-Cioclateu method, ranged up to 50.8 and up to 48.9 mg gallic acid/g for S. scardica and S. raeseri, respectively. Free radical scavenging activity was evaluated by DPPH assay and the activity, presented as IC50 values, ranged from 3.2-8.9 mg/mL and 7.6-12.6 mg/mL for S. scardica and S. raeseri, respectively. The content of twenty minerals in dried over-ground parts of the plants and in water tea-infusions were determined by the ICP-AES method and some of them alternatively by ETAAS. The most abundant minerals were K > Ca > Mg > P > Fe > Al > Na. The microelements and toxic elements contents were represented in the following order: Zn > Mn > B > Ba > Cu > Sr > Li > Ni > Cr > Co, and Cd > Pb > As, respectively. Water tea-infusions contained a large portion of the total K, P, Na, Cu and Pb, but smaller amounts of the other elements.

A New Chlorine-containing Glucosyl-fused Compound from Curculigo glabrescens

2009 ◽  
Vol 64 (9) ◽  
pp. 1077-1080 ◽  
Author(s):  
Cui-Cui Zhu ◽  
Tian-Ming Wang ◽  
Kai-Jin Wang ◽  
Ning Li
Keyword(s):  
Spectroscopic Analysis ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
2D Nmr ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Assay ◽  
Etoh Extract ◽  
Structure Activity ◽  
First Time

A new chlorine-containing glucosyl-fused compound, crassifoside H (1), was isolated from the EtOH extract of the rhizomes of Curculigo glabrescens. The structure was established on the basis of MS, IR, 1D and 2D NMR experiments. In addition, seven known compounds (2 - 8) were isolated and identified by spectroscopic analysis and comparison of their spectral data with those reported previously. All the compounds were isolated from this plant for the first time. The free radical scavenging activity of the isolated compounds was evaluated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. Compounds 1 and 2 showed strong radical scavenging activities. The primary structure-activity relationship is also discussed.

scholarly journals Comparative study of in vitro, ex vitro and in vivo grown plants of Arnica montana – polyphenols and free radical scavenging activity

2013 ◽  
Vol 72 (1) ◽  
pp. 13-22 ◽  
Author(s):  
Milena Nikolova ◽  
Mariya Petrova ◽  
Ely Zayova
Keyword(s):  
Free Radical ◽  
Methyl Ether ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Ex Vitro ◽  
Polyphenolic Content

Abstract Arnica montana L. is an endangered species rich in sesquiterpene lactones, phenolic acids and flavonoids with high pharmaceutical value. The polyphenolic content and free radical scavenging activity of plants that had passed all stages of cultivation: micropropagation and rooting (in vitro), adaptation in greenhouse (ex vitro) and mountain conditions (in vivo) were evaluated. Four surface flavonoid aglycones [scutellarein 6-methyl ether (hispidulin), scutellarein 6,4’-dimethyl ether (pectolinarigenin), 6-OH luteolin 6-methyl ether and kempferol-6-methyl ether] were detected in the acetone exudates of the studied samples bymeans of thin layer chromatography.No differences in the accumulation of surface flavonoids were found among the tested leaf extracts of in vitro, ex vitro and in vivo samples. However, the extracts from the flowers were richer in surface flavonoids than extracts from the leaves. The methanol extracts of the samples from ex vitro and in vivo grown A. montana plants had significantly higher radical scavenging activity and polyphenolic content than the extracts of in vitro samples. The observed differences in the contents of these biologically active compounds were related to different growth conditions and stages of plant development. The biotechnological method of A. montana established holds promise for the future production of antioxidants.

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