Non-Phenolic Antioxidant Compounds from Buddleja asiatica

2008 ◽  
Vol 63 (7-8) ◽  
pp. 483-491 ◽  
Author(s):  
Mortada M. El-Sayed ◽  
El-Sayed S. Abdel-Hameed ◽  
Wafaa S. Ahmed ◽  
Eman A. El-Wakil
Keyword(s):  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Compounds ◽  
Scavenging Activity ◽  
Chemical Analyses ◽  
Dpph Assay ◽  
Antioxidant Agents ◽  
Total Antioxidant

The methanol extract of the leaves of Buddleja asiatica Lour. (Loganiaceae) showed antioxidant activity toward the well known in vitro antioxidant tests such as total antioxidant capacity by the phosphomolybdenum method, free radical scavenging activity by the 1,1- diphenyl-2-picrylhydrazyl scavenging assay (DPPH assay) and hydrogen peroxide scavenging methods. Due to the high scavenging activity of the n-butanol successive fraction toward DPPH and H2O2 (SC50 = 11.99 and 18.54 μg/ml, respectively), this extract was subjected to chromatographic separation and isolation. Four non-phenolic compounds were isolated and identified on the basis of spectroscopic and chemical analyses: 1-O-ß-D-glucopyranosyl- 2-methoxy-3-(2-hydroxy-triaconta-3,12-dienoate)-glycerol (1), 3-O-[α-l-rhamnopyranosyl- (1→4)-ß-d-glucopyranosyl-(1→3)]-[ß-d-glucopyranosyl-(1→2)]-ß-d-fucopyranosyl-olean- 11,13(18)-diene-3ß,23,28-triol (2), 3-O-[α-l-rhamnopyranosyl-(1→4)-ß-d-glucopyranosyl- (1→4)-ß-d-glucopyranosyl-(1→3)]-ß-d-fucopyranosyl-olean-11,13(18)-diene-3ß,23,28-triol (3), and 3-O-[α-l-rhamnopyranosyl-(1→4)-ß-d-glucopyranosyl-(1→3)]-[ß-d-xylopyranosyl- (1→2)]-ß-d-glucuronopyranosyl-acid-olean-11,13(18)-diene-3ß,23,28-triol (4). The four compounds were evaluated as antioxidant agents using the three antioxidant bioassay tests.

scholarly journals Formulation Evaluation and In- Vitro Antioxidant activity of Microparticles of Syzygium Cumini Plant Extract

2021 ◽  
pp. 77-85
Author(s):  
Sweta Srivastava Koka ◽  
Pravin Kumar Sharma ◽  
Vaishali Sharma ◽  
Jaya Verma ◽  
G. N. Darwhekar
Keyword(s):  
Antioxidant Activity ◽  
Plant Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Flowering Plant ◽  
Dependent Manner ◽  
Scavenging Activity ◽  
Total Antioxidant ◽  
Antioxidant Supplement

Sygygium cumini, commonly known as Malabar plum, Java plum, black plum, jamun or jambolan, is an evergreen tropical tree in the flowering plant family Myrtaceae. The present study the antioxidant effects of microparticles of S. cumini plant extract were evaluated using in vitro, 2, 2-diphenylpicrylhydrazine photometric assay, total antioxidant activity and super oxide free radical scavenging activity. The microparticles showed different levels of radicals scavenging activity in a dose-dependent manner over the range of 50–150 µg/mL concentration, indicating the high antioxidative capacity of the extract. In all the experimental screening models microparticles of aqueous extract show the maximum inhibition of free radicals comparison of other experimental model at different concentrations. These findings suggest that the seed of Syginium cumini has potent antioxidant activity which may be responsible for some of its reported pharmacological activities and can be used as antioxidant supplement.

scholarly journals Evaluation of antioxidant activity of ormeloxifene: in vitro study

2017 ◽  
Vol 6 (10) ◽  
pp. 2507
Author(s):  
Vinay M. ◽  
Seethalakshmi S. ◽  
Vijay Kumar
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
No Synthase ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Dpph Assay

Background: Ormeloxifene (Centchroman) is a Selective Estrogen Receptor Modulator (SERM) which acts as estrogen antagonist and having anti progestogenic activity also. It is being used in the management of dysfunctional uterine bleeding and as nonhormonal oral contraceptive. It is also being investigated for the indications such as osteoporosis, breast and endometrial carcinoma. In this study, we have evaluated the Antioxidant potential of drug by using DPPH and NO synthase Assay. It was found that ormeloxifene has significant antioxidant activity which could be cause for its use in various gynaecological and other conditions.Methods: In this study, we have demonstrated in vitro antioxidant activity of ormeloxifene. DPPH and NO synthase assay tests were done for different concentrations of ormeloxifene.Results: In our study, it showed that the free radical scavenging activity of ormeloxifene was less in lower concentration and increased in the higher concentration in DPPH assay. The free radical scavenging activity of drug ormeloxifene was 22% at 100µg/ml and 27% for the concentrations of 1000µg/ml in DPPH assay. No scavenging activity was 3% at 100µg/ml and 11% at 1000µg/ml.Conclusions: The invitro antioxidant analysis of ormeloxifene, was proved to be a potent antioxidant.

Phytochemical constituents of Cardamine diphylla

2010 ◽  
Vol 88 (1) ◽  
pp. 50-55 ◽  
Author(s):  
Sabine Montaut ◽  
René S. Bleeker ◽  
Carine Jacques
Keyword(s):  
Brine Shrimp ◽  
Structure Elucidation ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Spectroscopic Methods ◽  
Scavenging Activity ◽  
Dpph Assay ◽  
Phytochemical Constituents

From the methanol extract of the Cardamine diphylla rhizome, methylethyl- (1), 2-methylbutyl- (2), 3-methylpentyl- (4), 3-indolylmethyl- (5), 1-methoxy-3-indolylmethyl- (6), 4-methoxy-3-indolylmethyl- (7) glucosinolates, and desulfo-2-methylbutylglucosinolate (3) were isolated. The structure elucidation of the compounds was performed by spectroscopic methods. The toxicity on brine shrimp larvae of the methanol extract of the C. diphylla rhizome was evaluated. In addition, the free-radical-scavenging activity of the crude extract was carried out by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay.

Phytochemical Screening and In Vitro Antioxidant Activity of Ethanolic Extract of Cassia occidentalis

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
S. Srividya ◽  
G. Sridevi ◽  
A. G. Manimegalai
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Cassia Occidentalis ◽  
Total Antioxidant ◽  
Anti Oxidant

The ethanolic extract of the leaves of Cassia occidentalis (Co) were subjected to phytochemical analysis by standard qualitative analysis and the invitro antioxidant activity was evaluated by determination of total antioxidant capacity, 1.1- diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, hydrogen peroxide (H2O2) radical scavenging activity, superoxide scavenging activity and Ferric reducing anti oxidant potential (FRAP). The analyses revealed that the ethanolic extract of Co was able to efficiently scavenge the free radicals in a dose dependant manner. The results were compared with the standard antioxidant ascorbic acid. The results have shown that crude ethanolic extract of the leaves of Co showed excellent antioxidant activity due to the presence of bioactive compounds namely alkaloids, betacyanin, cardiac glycosides, coumarins, flavonoids, phenols, steroids, saponins, tannins, terpenoids, anthraquinones and emodins.

scholarly journals EVALUATION OF IN VITRO ANTIOXIDANT ACTIVITY OF A MEDICINAL HERB, WEDELIA CHINENSIS (OSBECK) MERRILL.

2018 ◽  
Vol 11 (10) ◽  
pp. 433
Author(s):  
Rehana Banu H ◽  
Nagarajan N
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Total Antioxidant ◽  
Dpph Radical Scavenging ◽  
In Vitro Antioxidant Activity

Objective: The objective is to evaluate the antioxidant activity of the methanolic leaf extract of Wedelia chinensis.Methods: In vitro antioxidant activity was evaluated by studying 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, superoxide radical scavenging activity, ferric reducing ability of plasma (FRAP) scavenging activity, hydroxyl radical scavenging activity, metal chelating activity, and total antioxidant activity. Ascorbic acid, BHT, quercetin, and EDTA were used as standards for the experiments.Results: Inhibition concentration50 values observed for DPPH radical scavenging, superoxide radical scavenging, and hydroxyl radical scavenging activities were determined to be 62.5, 769.23, and 617.28 μg/ml, respectively and that of FRAP scavenging, metal chelating, and total antioxidant activities were found to be 343 μmol Fe (II)/g, 79.56 mg EDTA/g extract, and 398.17 mg ascorbic acid eq/g extract, respectively.Conclusion: The results clearly indicate that methanolic leaf extract of the study species W. chinensis is effective in scavenging free radicals and has the potential to be a powerful antioxidant.

scholarly journals Red SeaSuberea mollisSponge Extract Protects against CCl4-Induced Acute Liver Injury in Rats via an Antioxidant Mechanism

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Aymn T. Abbas ◽  
Nagla A. El-Shitany ◽  
Lamiaa A. Shaala ◽  
Soad S. Ali ◽  
Esam I. Azhar ◽  
...  
Keyword(s):  
Liver Injury ◽  
Acute Liver Injury ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Marine Sponges ◽  
Scavenging Activity ◽  
Dpph Assay ◽  
Antioxidant Mechanism ◽  
Histopathological Results

Recent studies have demonstrated that marine sponges and their active constituents exhibited several potential medical applications. This study aimed to evaluate the possible hepatoprotective role as well as the antioxidant effect of the Red SeaSuberea mollissponge extract (SMSE) on carbon tetrachloride- (CCl4-) induced acute liver injury in rats. In vitro antioxidant activity of SMSE was evaluated by 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay. Rats were orally administered three different concentrations (100, 200, and 400 mg/kg) of SMSE and silymarin (100 mg/kg) along with CCl4(1 mL/kg, i.p., every 72 hr) for 14 days. Plasma aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and total bilirubin were measured. Hepatic malondialdehyde (MDA), reduced glutathione (GSH), nitric oxide (NO), superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) were also measured. Liver specimens were histopathologically examined. SMSE showed strong scavenging activity against free radicals in DPPH assay. SMSE significantly reduced liver enzyme activities. Moreover, SMSE significantly reduced hepatic MDA formation. In addition, SMSE restored GSH, NO, SOD, GPx, and CAT. The histopathological results confirmed these findings. The results of this study suggested a potent protective effect of the SMSE against CCl4-induced hepatic injury. This may be due to its antioxidant and radical scavenging activity.

scholarly journals ANTIOXIDANT ACTIVITY OF LEAF EXTRACT FROM SIDA SUBCORDATA SPAN

2017 ◽  
Vol 1 (2) ◽  
Author(s):  
Rahma Yulia ◽  
Dachriyanus Dachriyanus ◽  
Amri Bachtiar
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Compounds ◽  
Spectrophotometric Methods ◽  
Dpph Method ◽  
Extract Fraction

This Study has tested the antioxidant activity of the extracts from Sida subcordata Span. Testing is done with antioxidant activity using DPPH method provides. Testing of antioxidant activity in vitro with spectrophotometric methods based on absorbance measurements radical DPPH (1,1-diphenylpicrylhydrazyl). All the extracts Showed radical scavenging activity. The ethyl acetate and methanol extracts were the most Appropriate as sources of antioxidant compounds as shown by Reviews their inhibition percentage (IP) values. Percentage inhibitor to extract ethyl acetate and methanol extract respectively 76.16% and 79.28% at a concentration of 1 mg / ml to 0.05 mM DPPH. while the results of the testing activities of antioxidant compounds y pure ethyl acetate extract fraction showed a smaller percentage inhibitor that is equal to 30% at the same concentration. Keyword - Sida subcordata Span, Malvaceae, Antioxidant, Alkaloid

scholarly journals Evaluation of in-Vitro antioxidant and antidiarrheal activities of Peperomia Pellucida methanol extracts on Albino mice

2021 ◽  
Vol 25 (9) ◽  
pp. 1681-1688
Author(s):  
O.D. Uwaya ◽  
P.O. Omozuwa ◽  
R.E. Inegbedion
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Scavenging Activity ◽  
Tropical Regions ◽  
Cholesterol Levels ◽  
Total Antioxidant ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Common Plant

Peperomia pellucida also known as shiny bush is a common plant wildly distributed in the tropical regions of the world including Nigeria. In ethnomedicine, it is used to treat hemorrhages, fevers, lower cholesterol levels and serves as a cough suppressant, emollient, and diuretic. This study evaluated the in vitro and antidiarrhea activities of Peperomia pellucida. For the in vitro antioxidant assay the following methods were used: 2-diphenyl-2-pierylhydrazylhydrate (DPPH) scavenging activity, Superoxide scavenging activity, Hydrogen peroxide scavenging activity, Lipid Peroxidation Assay, Total Antioxidant Capacity, 2,2’-Azinobis (3-Ethylbenzothiazoline-6-sulphonic Acid) (ABTS) radical scavenging activity and Hydroxyl radical scavenging activity. Antidiarrheal activities were studied using Castor Oil-Induced and Intestinal Transit in Mice models. Methanol and aqueous plant extract of Peperomia pellucida at various concentration demonstrated impressive in vitro antioxidant scavenging activities. The onset of diarrheal (**P<0.01) and the % of charcoal travel (*P<0.05) in the mice was reduced at 200 mg/kg compared to the control. Conclusively, this study showed that the aqueous and methanol extract of Peperomia pellucida has in vitro free radical scavenging activities. The plant demonstrated antidiarrheal activities.

scholarly journals In vitro and in vivo Antioxidant Properties of Extracts from the Root of Curcuma longa Linn

2020 ◽  
pp. 6-12
Author(s):  
Abdulrashid Mohammed ◽  
Muhammad Ibrahim Usman ◽  
Alhassan Muhammad Wudil ◽  
Adamu Jibrin Alhassan ◽  
Salisu Maiwada Abubakar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Curcuma Longa ◽  
Radical Scavenging ◽  
Diabetic Rats ◽  
Scavenging Activity ◽  
Liver And Kidney

Many plants possess antioxidants that exhibit additive or synergistic activities. The antioxidant activities of the root of Curcuma longa Linn extracts extracted different solvents were investigated by using several established in vitro systems: α,α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging activity, hydrogen Peroxide scavenging activity (HPSA), nitric oxide radical scavenging activity (NOSA) and ferric reducing antioxidant power (FRAP). The result showed that methanol extract exhibited greater antioxidant activity in vitro which was statistically significant compared to the other extracts. Based on the in vitro results, the methanol extract was subjected to column chromatography. Six pooled fractions (FI-FVI) were evaluated for in vivo antioxidant activity in liver and kidney of alloxan-induced diabetic rats using a total of forty-five (45) rats which were grouped into nine (9) groups of five (5) rats. The in vivo antioxidants showed a significant decrease in superoxide dismutase (SOD), catalase (CAT) and gluthatione peroxidase (GPx) levels in both liver and kidney of Alloxan-induced diabetic rats. These changes were significantly reversed after treatment with methanol fraction II and the standard drug. Thus, Curcuma longa Linn may be useful in the management of diabetes and oxidative stress.

scholarly journals Total Phenolic Content and Antioxidant Activity of Two Teucrium Species from Syria -

2020 ◽  
Vol 10 (1) ◽  
pp. 41-45
Author(s):  
Reem Ghassan Alali ◽  
Mays Rizk Khazem
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Total Phenolic ◽  
Natural Food ◽  
Antioxidant Compounds ◽  
Scavenging Activity ◽  
Total Polyphenols

Germander (Teucrium, Lamiaceae) is a genus commonly used as a medicinal herb in the middle east. The aim of this study was to estimate total polyphenols and antioxidant activity of different extracts from two Syrian Teucrium species (Teucrium polium and Teucrium creticum). Total polyphenols content was measured using Folin-Ciocalteu assay and the antioxidant activity was evaluated in vitro by DPPH assay (free radical scavenging activity against 1, 1 –diphenyl-2-picrylhydrazyl). The total polyphenols content and antioxidant activity of T. creticum extracts were higher than T. polium ones. Phenols contents ranged from 16.388 to 105.672 mg GA E/ g dry weight extract. The extracts showed significant scavenging activity against DPPH, with IC50 values ranging between 0.195 and 0.710 mg/ml. The methanolic extracts show higher antioxidant activity from the two species. A good correlation between DPPH scavenging activities and contents of polyphenols was found. These results show that Syrian Teucrium species, especially T. creticum least Known among all, is a rich source of phenols and natural antioxidant compounds, that can be used as a natural food preservative.

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