scholarly journals Anti-Inflammatory Activities of Ethyl Acetate Fraction From Uncaria Gambir Leaves Through the Inhibition of Edema, COX-2 and iNOS Expression

2020 ◽  
Author(s):  
Nanang Yunarto ◽  
Putri Reno Intan ◽  
Arifayu Addiena Kurniatri ◽  
Indah Sulistyowati ◽  
Nurul Aini
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Inos Expression ◽  
Cox 2 ◽  
Anti Inflammatory

Chemical Composition, Antioxidant and Anti-inflammatory Activity Evaluation of the Lebanese Propolis Extract

2019 ◽  
Vol 20 (1) ◽  
pp. 84-96 ◽  
Author(s):  
Rawan Zeitoun ◽  
Fadia Najjar ◽  
Batoul Wehbi ◽  
Alia Khalil ◽  
Mohammad Fayyad-Kazan ◽  
...  
Keyword(s):  
Chemical Analysis ◽  
Protein Expression ◽  
Ethyl Acetate ◽  
Antioxidant Properties ◽  
Ethyl Acetate Fraction ◽  
Chemical Compositions ◽  
Inos Protein ◽  
Inos Protein Expression ◽  
Cox 2 ◽  
Anti Inflammatory

Background: Propolis is a resinous substance produced by bees and known to possess antioxidant, antimicrobial, antiproliferative and anti-inflammatory activities. Objective: This study is aimed at evaluating the in vivo and in vitro anti-inflammatory potential of the Crude Ethanolic Extract (CE) of Lebanese propolis and its Ethyl Acetate Fraction (EAF). Method: Chemical content of propolis was characterized using high-performance liquid chromatography and LC-MS/MS. COX-2 and iNOS protein expression, nitric oxide (NO) and prostaglandin (PGE2) release in LPS-activated RAW monocytes were achieved respectively by western blot and spectrophotometry. Antioxidant activity was evaluated by DPPH free radical scavenging assay. Measurement of paw thickness in carrageenan-induced paw edema in mice and pathologic assessment of inflammation in paw sections were used to judge the anti-inflammatory properties of propolis. Results: Pathology analysis revealed in the treated group significant reduction of immune cell infiltration and edema. Both extract and ethyl acetate fraction showed significant anti-inflammatory and antioxidant effects in LPS-treated RAW cells characterized by the inhibition of COX-2 and iNOS protein expression, as well as PGE2 and NO release. Chemical analysis of the crude extract and its ethyl acetate fraction identified 28 different compounds of which two phenolic acids and nine other flavonoids were also quantified. Ferulic acid, caffeic acid, chrysin, galangin, quercetin, and pinocembrin were among the most representative compounds. Conclusion: Lebanese propolis is rich in a various amount of flavonoids which showed promising antiinflammatory and antioxidant properties. Additionally, chemical analysis showed unique chemical compositions with the potential of identifying ingredients with interesting anti-inflammatory activities.

Anti-inflammatory potential of an ethyl acetate fraction isolated from Justicia gendarussa roots through inhibition of iNOS and COX-2 expression via NF-κB pathway

2012 ◽  
Vol 272 (2) ◽  
pp. 283-289 ◽  
Author(s):  
Kavitha S. Kumar ◽  
Viji Vijayan ◽  
Shobha Bhaskar ◽  
Kripa Krishnan ◽  
V. Shalini ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Cox 2 ◽  
Anti Inflammatory

Anti-inflammatory activity of ethyl acetate fraction of the seeds of Brucea Javanica

2013 ◽  
Vol 147 (2) ◽  
pp. 442-446 ◽  
Author(s):  
Jianhong Yang ◽  
Shucai Li ◽  
Caifeng Xie ◽  
Haoyu Ye ◽  
Huan Tang ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Inflammatory Activity ◽  
Brucea Javanica ◽  
Anti Inflammatory

scholarly journals Anti-Inflammatory Potential of Ethyl Acetate Fraction of Moringa oleifera in Downregulating the NF-κB Signaling Pathway in Lipopolysaccharide-Stimulated Macrophages

Molecules ◽  
2016 ◽  
Vol 21 (11) ◽  
pp. 1452 ◽  
Author(s):  
Palanisamy Arulselvan ◽  
Woan Tan ◽  
Sivapragasam Gothai ◽  
Katyakyini Muniandy ◽  
Sharida Fakurazi ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Signaling Pathway ◽  
Moringa Oleifera ◽  
Ethyl Acetate Fraction ◽  
Anti Inflammatory

scholarly journals Ethyl Acetate Fraction from Hedyotis diffusa plus Scutellaria barbata Exerts Anti-Inflammatory Effects by Regulating miR-155 Expression and JNK Signaling Pathway

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Yuan Xu ◽  
Xiao-Xia Chen ◽  
Yi-Xin Jiang ◽  
Dan-Dan Zhang
Keyword(s):  
Ethyl Acetate ◽  
Signaling Pathway ◽  
Ethyl Acetate Fraction ◽  
Equal Weight ◽  
Dependent Manner ◽  
Jnk Signaling ◽  
Scutellaria Barbata ◽  
Hedyotis Diffusa ◽  
Jnk Signaling Pathway ◽  
Anti Inflammatory

Hedyotis diffusa Willd and Scutellaria barbata D. Don (HDSB) were the core couplet in medicines that were commonly used for the purpose of anti-inflammation and anticancer treatments in China. However, biological properties of this couplet have not been fully elucidated. In this study, we screened fractions of HDSB for their anti-inflammatory activities and explored pertinent molecular mechanisms on murine macrophage RAW264.7 cell model. Ethyl acetate fraction from the aqueous extract of the couplet at equal weight ratio (EA11) showed the strongest inhibition of the nitrite accumulation in supernatant of RAW264.7 cells stimulated with lipopolysaccharide (LPS)/interferon-γ (IFN-γ). In addition, EA11 inhibited iNOS and IL-1β expression in a concentration-dependent manner while promoting the expression of HO-1 and PPAR-γ. Anti-inflammatory capability is most likely facilitated by its inhibitory effect on JNK signaling pathway and miR-155 expression. This study suggests that EA11 may be represented as a potential anti-inflammatory therapeutic candidate.

Antinociceptive and anti-inflammatory activities of ethyl acetate fraction from Zanthoxylum armatum in mice

Fitoterapia ◽  
2011 ◽  
Vol 82 (3) ◽  
pp. 347-351 ◽  
Author(s):  
Tao Guo ◽  
Yun-Xia Deng ◽  
Hui Xie ◽  
Chun-Yan Yao ◽  
Cheng-Cheng Cai ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Zanthoxylum Armatum ◽  
Anti Inflammatory

scholarly journals Antimycobacterial and Nitric Oxide Production Inhibitory Activities ofOcotea notatafrom Brazilian Restinga

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Isabela Francisca Borges Costa ◽  
Sanderson Dias Calixto ◽  
Marlon Heggdorne de Araujo ◽  
Tatiana Ungaretti Paleo Konno ◽  
Luzineide Wanderley Tinoco ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
National Park ◽  
Antimycobacterial Activity ◽  
Ethyl Acetate Fraction ◽  
No Production ◽  
Nitric Oxide Production ◽  
Phytochemical Investigation ◽  
Inhibitory Activities ◽  
Cox 2 ◽  
Cox 1

The genusOcotea(Lauraceae) is distributed mainly in tropical and subtropical regions. Some species of this genus asO. puberulaandO. quixoshave been described in the literature, showing antibacterial activity. AndOcotea macrophyllashowed anti-inflammatory activity with inhibition of COX-1, COX-2, and LOX-5. The purpose of this study was the phytochemical investigation of the plant speciesOcotea notatafrom Restinga Jurubatiba National Park, Macaé, RJ, Brazil, and the search for antimycobacterial fractions and compounds. The crude extract was evaluated for antimycobacterial activity and presented95.75±2.53% of growth inhibition at 100 µg/mL. Then, it was subjected to a liquid-liquid partition and subsequently was chemically investigated by HPLC, revealing the major presence of flavonoids. In this process the partition fractions hexane, ethyl acetate, and butanol are shown to be promising in the antimycobacterial assay. In addition, ethyl acetate fraction was chromatographed and afforded two flavonoids identified by MS and NMR as afzelin and isoquercitrin. The isolated flavonoids afzelin and isoquercitrin were evaluated for their antimycobacterial activity and for their ability to inhibit NO production by macrophages stimulated by LPS; both flavonoids isoquercitrin (Acet22) and afzelin (Acet32) were able to inhibit the production of NO by macrophages. The calculated IC50of Acet22 and Acet32 was 1.03 and 0.85 µg/mL, respectively.

Anti-Oxidative and Anti-Inflammatory Effects of Lobelia chinensis In Vitro and In Vivo

2015 ◽  
Vol 43 (02) ◽  
pp. 269-287 ◽  
Author(s):  
Kun-Cheng Li ◽  
Yu-Ling Ho ◽  
Guan-Jhong Huang ◽  
Yuan-Shiun Chang
Keyword(s):  
Nitric Oxide ◽  
Folk Medicine ◽  
Ethyl Acetate Fraction ◽  
Raw264.7 Macrophages ◽  
Tnf Α ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Factor Α

Lobelia chinensis Lour (LcL) is a popular herb that has been widely used as folk medicine in China for the treatment of fever, lung cancer, and inflammation for hundreds of years. Recently, several studies have shown that the anti-inflammatory properties were correlated with the inhibition of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) from the NF-κB pathway. The aim of this study was to evaluate the anti-oxidative and anti-inflammatory activities of L. chinensis. Both suppressive activities on LPS-induced nitric oxide production in RAW264.7 macrophages in vitro and the acute rat lung injury model in vivo were studied. The results showed that the methanol extract of LcL and its fractions within the range of 62.5–250 μg/mL did not induce cytotoxicity (p < 0.001). The ethyl acetate fraction of LcL showed better NO inhibition activity than other fractions. On the other hand, the Lc-EA (62.5, 125, 250 mg/kg) pretreated rats showed a decrease in the pro-inflammatory cytokines (TNF-α, IL-β, IL-6) and inhibited iNOS, COX-2 expression through the NF-κB pathway. These results suggested that L. chinensis exhibited an anti-inflammatory effect through the NF-κB pathways.

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