scholarly journals Two Derivative Spectrophotometric Methods for the Simultaneous Determination of 4-AminoAntipyrine in Presence of Its Acidic Products

2018 ◽  
Vol 31 (1) ◽  
pp. 114
Author(s):  
Ruba Fahmi Abbas
Keyword(s):  
Correlation Coefficient ◽  
Limit Of Detection ◽  
Coefficient Of Determination ◽  
Second Derivative ◽  
Zero Crossing ◽  
Spectrophotometric Methods ◽  
Ratio Spectrum ◽  
First Derivative ◽  
Normal Ratio ◽  
Recovery Percentage

    Simple, economic and sensitive mathematical spectrophotometric methods were developed for the estimation 4-aminoantipyrine in presence of its acidic product. The estimation of binary mixture 4-aminoantipyrine and its acidic product was achieved by first derivative and second derivative spectrophotometric methods by applying zero-crossing at (valley 255.9nm and 234.5nm) for 4-aminoantipyrine and (peak 243.3 nm and 227.3nm) for acidic product. The value of  coefficient of determination  for the liner  graphs were  not less than 0.996 and the recovery percentage were found to be in the range from 96.555 to 102.160. Normal ratio spectrophotometric method 0DD was used 50 mg/l acidic product as a divisor and then measured at 299.9 nm with correlation coefficient 0.998 and limit of detection 0.04098. ratio derivative methods 1DD and 2DD; are based on measuring the first derivative and second derivative for normal ratio spectrum at (peak 290.7 nm and valley 310 nm) for 1DD and(peak 286, valley 301 and peak 316nm) for 2DD the correlation coefficient for linearity graph not less than 0.997 and the recovery percentage were found to be in the range from 99.64 to 100.11.

scholarly journals Two Derivative Spectrophotometric Methods for the Simultaneous Determination of 4-AminoAntipyrine in Presence of Its Acidic Products

2018 ◽  
Vol 31 (2) ◽  
pp. 86
Author(s):  
Ruba Fahmi Abbas
Keyword(s):  
Correlation Coefficient ◽  
Limit Of Detection ◽  
Coefficient Of Determination ◽  
Second Derivative ◽  
Zero Crossing ◽  
Spectrophotometric Methods ◽  
Ratio Spectrum ◽  
First Derivative ◽  
Normal Ratio ◽  
Recovery Percentage

Simple, economic and sensitive mathematical spectrophotometric methods were developed for the estimation 4-aminoantipyrine in presence of its acidic product. The estimation of binary mixture 4-aminoantipyrine and its acidic product was achieved by first derivative and second derivative spectrophotometric methods by applying zero-crossing at (valley 255.9nm and 234.5nm) for 4-aminoantipyrine and (peak 243.3 nm and 227.3nm) for acidic product. The value of coefficient of determination for the liner graphs were not less than 0.996 and the recovery percentage were found to be in the range from 96.555 to 102.160. Normal ratio spectrophotometric method 0DD was used 50 mg/l acidic product as a divisor and then measured at 299.9 nm with correlation coefficient 0.998 and limit of detection 0.04098. ratio derivative methods 1DD and 2DD; are based on measuring the first derivative and second derivative for normal ratio spectrum at (peak 290.7 nm and valley 310 nm) for 1DD and (peak 286, valley 301 and peak 316nm) for 2DD the correlation coefficient for linearity graph not less than 0.997 and the recovery percentage were found to be in the range from 99.64 to 100.11.

scholarly journals DEVELOPMENT OF METHODS FOR DETERMINING THEOPHYLLINE, SALBUTAMOL SULFATE AND GLYCERYLGUAIACOLATE IN A SIMULTANNEOUS MULTICOMPONENT SYRUP PREPARATION USING ULTRAVIOLET SPECTROPHOTOMETRY DERIVATIVE

2019 ◽  
Author(s):  
Nur Tsalits Fahman Mughni
Keyword(s):  
Limit Of Detection ◽  
Drug Preparation ◽  
Second Derivative ◽  
Salbutamol Sulfate ◽  
Ultraviolet Spectrophotometry ◽  
Limit Of Quantification ◽  
Zero Crossing ◽  
First Derivative ◽  
Relative Standard ◽  
Active Substances

Theophylline, salbutamol sulfate and glycerylguaiacolate are active substances for treat asthma (dyspnoea). Combination theophylline, salbutamol sulfate and glycerylguaiacolate in drug preparation have to produce pharmacological effect, so the drug preparation needs the quality assurance of product. This study aims to determine theophylline, salbutamol sulfate and glycerylguaiacolate using ultraviolet spectrophotometry derivative. Research has been conducted in syrup was analyzed using zero crossing wavelength method. NaOH 0,1 N was selected as the solvent. Zero crossing wavelength of theophylline is 275 nm on the first derivative, salbutamol sulfate 262 nm on the second derivative and glycerylguaiacolate 243 nm on the second derivative. Results showed that the recovery of theophylline and glycerylguaiacolate are between 112,913%-118,353% and 116,129%-117,655%, respectively, while the relative standard deviation between 1,090%-1,903% for theophylline and 1,013%-1,922% for glycerylguaiacolate. Limit of detection of theophylline and glycerylguaiacolate are 1,626 ppm and 6,375 ppm, respectively while limit of quantification are 4,927 ppm and 19,319 ppm. So, it concluded that this method could be applied to determine theophylline and glycerylguaiacolate in syrup mixture.

scholarly journals VALIDATED SPECTROPHOTOMETRIC METHODS FOR THE ESTIMATION OF CINNARIZINE IN BINARY MIXTURE WITH PARACETAMOL IN BULK AND TABLETS

2021 ◽  
pp. 161-166
Author(s):  
AHMED M AL-GHANI ◽  
ANES AM THABET
Keyword(s):  
Quality Control ◽  
Second Derivative ◽  
Derivative Spectrum ◽  
Zero Crossing ◽  
Spectrophotometric Methods ◽  
First Derivative ◽  
Derivative Method ◽  
Analytical Tools ◽  
Second Derivative Method

Objectives: The aim of this work was to develop and validate new, simple, accurate, and selective spectrophotometric methods (derivative and derivative ratio spectrophotometric methods) for the determination of these drugs. These methods can be used as analytical tools in routine examination in quality control laboratories. Methods: The first method was derivative method in which the first derivative method (1D) for determination of PCM and the second derivative method (2D) for determination of CIN. The second method was the first derivative ratio spectrophotometric method (1DD) for determination of CIN and PCM. Results: In first method, the first derivative spectrum (1D) of PCM where PCM was determined by measuring the amplitude of the valley at 235 nm while CIN showed zero crossing spectrum, and the second derivative spectrum (2D) of CIN where CIN was determined by measuring the amplitude of the peak at 287.5 nm while PCM showed a zero value. In the second method, the first derivative ratio spectrophotometry (1DD) for CIN and PCM determination, where the amplitude at 290 and 291 nm, was selected for the determination of CIN and PCM, respectively. Conclusions: The developed methods were applied for the determination of the cited drugs in tablets containing binary drug mixture. The methods are simple and precise and can be used for routine analysis of the labeled drugs in combined dosage forms in quality control laboratories.

scholarly journals Three Different Spectrophotometric Methods for Simultaneous Determination of Pyriproxyfen and Chlorothalonil Residues in Cucumber and Cabbage Samples

2019 ◽  
Vol 2019 ◽  
pp. 1-11
Author(s):  
Shilan A. Omer ◽  
Nabil A. Fakhre
Keyword(s):  
Simultaneous Determination ◽  
Simultaneous Estimation ◽  
Zero Crossing ◽  
Spectrophotometric Methods ◽  
First Derivative ◽  
The Third ◽  
Mean Centering ◽  
Relative Standard ◽  
One Way Anova

In this study, three simple and accurate spectrophotometric methods for simultaneous determination of pyriproxyfen and chlorothalonil residues in cucumbers and cabbages grown in experimental greenhouse were studied. The first method was based on the zero-crossing technique measurement for first and second derivative spectrophotometry. The second method was based on the first derivative of the ratio spectra. However, the third method was based on mean centering of ratio spectra. These procedures lack any previous separation steps. The calibration curves for three spectrophotometric methods are linear in the concentration range of 1–30 μg·mL−1 and 0.5–7 μg·mL−1 for pyriproxyfen and chlorothalonil successively. The recoveries ranged from 82.12–97.40% for pyriproxyfen and 81.51–97.04% for chlorothalonil with relative standard deviations less than 4.95% and 5.45% in all instances for pyriproxyfen and chlorothalonil, respectively. The results obtained from the proposed methods were compared statistically by using one-way ANOVA, and the results revealed there were no significant differences between ratio spectra and mean centering methods with the zero-crossing technique. The proposed methods are successfully applied for the simultaneous estimation of the residue of both pesticides in cucumber and cabbage samples.

Spectrophotometric Method To Determine Ternary Mixtures of Atrazine, Diuron, and Chlorpyrifos in Water and Soil by a Ratio Spectrum-Zero Crossing Method

1995 ◽  
Vol 78 (2) ◽  
pp. 423-430 ◽  
Author(s):  
M Martínez Galera ◽  
J L Martínez Vidal ◽  
A Garrido Frenich ◽  
P Parrilla Vázquez
Keyword(s):  
Spectrophotometric Method ◽  
Ternary Mixtures ◽  
Zero Crossing ◽  
Ratio Spectrum ◽  
First Derivative ◽  
Simultaneous Use ◽  
Pesticides In Groundwater

Abstract A spectrophotometric method based on simultaneous use of first derivative of ratio spectra and measurement of zero-crossing wavelengths resolves ternary mixtures of atrazine, diuron, and chlorpyrifos. Calibration graphs were linear for atrazine between 1 and 15 μg/mL and for diuron and chlorpyrifos between 1 and 10 μg/mL. The pesticides in groundwater and soil were determined by the method, at part-per-billion levels, after solidphase extraction, with good results. Interferences caused by other pesticides were studied.

scholarly journals SIMULTANEOUS ESTIMATION OF ASPIRIN AND OMEPRAZOLE IN LABORATORY SAMPLE BY DIFFERENT UV SPECTROPHOTOMETRIC TECHNIQUES

INDIAN DRUGS ◽  
2020 ◽  
Vol 57 (09) ◽  
pp. 75-81
Author(s):  
Rakesh M Wani ◽  
Arun M Kashid ◽  
Amita Ghatge ◽  
Sumita Sahoo
Keyword(s):  
Simultaneous Equation ◽  
Ratio Method ◽  
Simultaneous Estimation ◽  
Laboratory Sample ◽  
Zero Crossing ◽  
Spectrophotometric Methods ◽  
First Derivative ◽  
First Derivative Spectrophotometry ◽  
Curve Method ◽  
Crossing Point

The aim of the present work was to develop simple, precise and economic UV- spectrophotometric methods for the simultaneous estimation of aspirin and omeprazole in a laboratory sample. The absorbance maxima (λmax) for detection of aspirin and omeprazole were selected as 274 nm and 302 nm, respectively, for simultaneous equation method while wavelength range for detection of aspirin and omeprazole were selected as 270 nm - 276 nm and 300 nm - 305 nm, respectively for area under curve method. Absorbance ratio method uses the ratio of absorbances at two selected wavelengths, one which is an isoabsorptive point and other being the λ max of one of the two components. From the overlay spectra of two drugs, it is evident that aspirin and omeprazole show an isoabsorptive point at 238.6 nm. In zero crossing first derivative spectrophotometry, aspirin showed zero crossing point at 301nm and omeprazole showed zero crossing point at 274nm. Linearity for aspirin was between 25- 125 μg/mL and omeprazole it was 3-15 μg/mL. These methods were successfully applied for estimation of aspirin and omeprazole in the laboratory sample.

scholarly journals Development of Ecofriendly Derivative Spectrophotometric Methods for the Simultaneous Quantitative Analysis of Remogliflozin and Vildagliptin from Formulation

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6160
Author(s):  
Mahesh Attimarad ◽  
Katharigatta N. Venugopala ◽  
Bandar E. Al-Dhubiab ◽  
Rafea Elamin Elgack Elgorashe ◽  
Sheeba Shafi
Keyword(s):  
Pharmaceutical Preparations ◽  
Peak Height ◽  
Zero Crossing ◽  
Spectrophotometric Methods ◽  
First Derivative ◽  
Relative Standard ◽  
Quality Control Study ◽  
Pharmaceutical Industries ◽  
Control Study

Three rapid, accurate, and ecofriendly processed spectrophotometric methods were validated for the concurrent quantification of remogliflozin (RGE) and vildagliptin (VGN) from formulations using water as dilution solvent. The three methods developed were based on the calculation of the peak height of the first derivative absorption spectra at zero-crossing points, the peak amplitude difference at selected wavelengths of the peak and valley of the ratio spectra, and the peak height of the ratio first derivative spectra. All three methods were validated adapting the ICH regulations. Both the analytes showed a worthy linearity in the concentration of 1 to 60 µg/mL and 2 to 90 µg/mL for VGN and RGE, respectively, with an exceptional regression coefficient (r2 ≥ 0.999). The developed methods demonstrated an excellent recovery (98.00% to 102%), a lower percent relative standard deviation, and a relative error (less than ±2%), confirming the specificity, precision, and accuracy of the proposed methods. In addition, validated spectrophotometric methods were commendably employed for the simultaneous determination of VGN and RGE from solutions prepared in the laboratory and the formulation. Hence, these methods can be utilized for the routine quality control study of the pharmaceutical preparations of VGN and RGE in pharmaceutical industries and laboratories. The ecofriendly nature of the anticipated spectrophotometric procedures was confirmed by the evaluation of the greenness profile by a semi-quantitative method and the quantitative and qualitative green analytical procedure index (GAPI) method.

scholarly journals Determination of Metformin Hydrochloride and Glyburide in an Antihyperglycemic Binary Mixture Using High-Performance Liquid Chromatographic-UV and Spectrometric Methods

2010 ◽  
Vol 93 (1) ◽  
pp. 133-140 ◽  
Author(s):  
Hesham Salem
Keyword(s):  
Binary Mixture ◽  
High Performance ◽  
Pharmaceutical Formulations ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Metformin Hydrochloride ◽  
Second Derivative ◽  
Zero Crossing ◽  
First Derivative

Abstract Three methods were developed for simultaneous determination of metformin hydrochloride and glyburide in an antihyperglycemic binary mixture without previous separation. In the first method, a reversed-phase HPLC column with acetonitrilewater (60 + 40, v/v) mobile phase at 0.9 mL/min flow rate was used to separate both compounds, with UV detection at 254 nm. Linearity was obtained in the concentration range of 0.060.24 µg/mL for glyburide and 1.56.0 µg/mL for metformin hydrochloride. The second method depended on first- and second-derivative UV spectrometry with zero-crossing measurements. The first-derivative amplitude at 261 nm was selected for the assay of glyburide, and the second-derivative amplitude at 235 nm was selected for the assay of metformin hydrochloride. The third method depended on measuring the first derivative of the ratio-spectra at 241 nm for glyburide and 227 nm for metformin hydrochloride. For the second and third methods, Beer's law was obeyed in the range of 1055 µg/mL for glyburide and 20200 µg/mL for metformin. The proposed methods were extensively validated and applied for the analysis of some pharmaceutical formulations containing binary mixtures of the mentioned drugs.

scholarly journals UV Spectrophotometric Methods to Quantify Alogliptin Benzoate and Pioglitazone Hydrochloride

2021 ◽  
pp. 31-41
Author(s):  
Dhanya B. Sen ◽  
Ashim K. Sen ◽  
Aarti S. Zanwar ◽  
Harshita Pandey ◽  
Rajesh A. Maheshwari
Keyword(s):  
Limit Of Detection ◽  
Spectroscopic Method ◽  
Simultaneous Estimation ◽  
Control Analysis ◽  
Limit Of Quantification ◽  
Spectrophotometric Methods ◽  
First Derivative ◽  
Ich Guidelines ◽  
The Difference ◽  
Tablet Dosage

Three new, precise, accurate and sensitive UV-Spectrophotometric methods namely Ratio Difference Spectroscopic Method (RDSM), First Derivative of Ratio Spectra Method (DR1) and Area Under Curve Method (AUC) were developed and validated for simultaneous assessment of alogliptin benzoate (ALO) and pioglitazone hydrochloride (PIO) in tablet dosage form. In RDSM, ratio spectra of both the drugs were recorded by dividing the mixtures using interfering drug as divisor. Then the difference between the amplitudes of obtained ratio spectra was measured at 288 and 291 nm for ALO and 236 and 245 nm for PIO. The second method DR1, where the first derivative of ratio spectra of both the drugs were recorded and the first derivative signal was measured at 290 nm for ALO and 276.8 nm for PIO. The scaling factor was fixed as 1 and wavelength interval (Δλ) as 2 for recording the first derivative of ratio spectra.  In the third method (AUC), peak area of recorded zero order spectra was measured at 276 ± 10 nm for ALO and 267.8 ± 10 nm for PIO. All three proposed methods were validated according to “International Conference on Harmonization” (ICH) guidelines parameters. For all three methods, ALO and PIO obeyed Beer’s law in the range of 0.5-5 & 1.8-18 µg/ml, respectively. The % RSD of repeatability of measurement, intra-day and inter-day precision were found to be less than 2 for all three methods. Limit of detection (LOD) and Limit of quantification (LOQ) of the drugs were calculated which proved the sensitivity of the methods. The accuracy ranged between 98-101% for all three methods. No interference from pharmaceutical excipients present in the formulation was observed.  These proposed methods were found to be simple, sensitive, accurate and precise and can be applied to the simultaneous estimation of ALO and PIO in combined tablet formulation and also appropriate for routine quality control analysis.

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