scholarly journals Molecular detection of Mycoplasma agalactiae and Mycoplasma capricolum in mastitic and non mastitic milk of goats by using Real Time Polymerase Chain Reaction

2018 ◽  
Vol 42 (1) ◽  
pp. 1-6
Author(s):  
Mohanad M. Jameel ◽  
Saleem M. Hasso
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Clinical Mastitis ◽  
Chain Reaction ◽  
Mycoplasma Agalactiae ◽  
Milk Samples ◽  
California Mastitis Test ◽  
Mycoplasma Capricolum ◽  
Acute Mastitis ◽  
Polymerase Chain

     A total of 200 samples were collected from 100 goats from more than one regions within the three governorates (Sulaimani, Erbil and Duhok); all these samples were tested by California mastitis test and these showed the following results: 103 samples (51.50%) were negative in all governorates, 28(14.00%) were trace, 35(17.50%) were (+) mark and 34(17.00%) were (++) mark. Clinical mastitis was observed on 93 out of 200 half udder of goats, in the three governorates; 37(18.5%) were acute mastitis, 27(13.5%) were sub acute mastitis and 19(9.5%) were Chronic mastitis. Examination of extracted DNA by Real Time Polymerase Chain Reaction out of 200 DNA extracted from milk samples of goats only 10 (5.00%) were positive for Mycoplasma agalactiae in the three governorates, 8(80%) from mastatic milk samples and 2 (20%) from non mastatic milk samples in result of M. agalactiae. For Mycoplasma capricolum 6 (3.00%) were positive and all were from mastatic milk.

scholarly journals Detection and enumeration of Staphylococcus aureus from bovine milk samples by real-time polymerase chain reaction

2013 ◽  
Vol 96 (11) ◽  
pp. 6955-6964 ◽  
Author(s):  
B.G. Botaro ◽  
C.S. Cortinhas ◽  
L.V. Março ◽  
J.F.G. Moreno ◽  
L.F.P. Silva ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Bovine Milk ◽  
Chain Reaction ◽  
Milk Samples ◽  
Polymerase Chain

Detection and Enumeration of Streptococcus agalactiae from Bovine Milk Samples by Real-Time Polymerase Chain Reaction

2015 ◽  
Vol 71 (3) ◽  
pp. 363-372 ◽  
Author(s):  
Nara Ladeira de Carvalho ◽  
Juliano Leonel Gonçalves ◽  
Bruno Garcia Botaro ◽  
Luis Felipe de Prada e Silva ◽  
Marcos Veiga dos Santos
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Streptococcus Agalactiae ◽  
Bovine Milk ◽  
Chain Reaction ◽  
Milk Samples ◽  
Polymerase Chain

Detection of clinical bovine mastitis caused by Mycoplasma bovis in Brazil

2020 ◽  
Vol 87 (3) ◽  
pp. 306-308
Author(s):  
Nathália Brancato Junqueira ◽  
Anelise Salina ◽  
Gabriela Capriogli Oliveira ◽  
Elena Mettifogo ◽  
Sâmea Fernandes Joaquim ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Bovine Mastitis ◽  
Dairy Farms ◽  
Clinical Mastitis ◽  
Dairy Herds ◽  
Mycoplasma Bovis ◽  
Chain Reaction ◽  
Research Communication ◽  
Milk Samples ◽  
Polymerase Chain

AbstractThe work reported in this research communication investigated the occurrence of Mycoplasma bovis (M. bovis) in milk samples from cows with clinical mastitis on dairy farms from seven Brazilian states. We hypothesized that M. bovis was present in bovine clinical mastitis milk in Brazil. A total of 561 milk samples were cultured on Hayflick agar and incubated in a microaerophilic atmosphere at 5% CO2. Polymerase chain reaction (PCR) was performed for the detection of Mycoplasma spp. and Mycoplasma bovis in milk samples. Mycoplasma spp. were isolated in 2% of the milk samples, and Mycoplasma bovis was verified in 3% of the milk samples by PCR. The results showed that Mycoplasma bovis is involved in clinical mastitis in Brazilian dairy herds. We emphasize the need for further studies to investigate the infection by this agent in clinical mastitis cases, particularly in Brazil, due to the lack of knowledge about its prevalence.

Detection of Mycoplasma agalactiae in sheep milk samples by polymerase chain reaction

1997 ◽  
Vol 54 (1) ◽  
pp. 17-22 ◽  
Author(s):  
S. Tola ◽  
A. Angioi ◽  
A.M. Rocchigiani ◽  
G. Idini ◽  
D. Manunta ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Mycoplasma Agalactiae ◽  
Milk Samples ◽  
Sheep Milk ◽  
Polymerase Chain

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain
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