Development and Validation of Derivative Spectroscopic Method for Simultaneous Estimation of Cefadroxil and Probenecid

2014 ◽  
Vol 7 (1) ◽  
pp. 2350-2355
Author(s):  
Pratik S Mehta ◽  
Pratik R. Patel ◽  
Rajesh R Parmar ◽  
M M K Modasiya ◽  
Dushyant A Shah
Keyword(s):  
Spectroscopic Method ◽  
Spectral Interference ◽  
Synthetic Mixture ◽  
Simultaneous Estimation ◽  
Zero Crossing ◽  
First Order ◽  
Derivative Spectroscopy ◽  
Development And Validation ◽  
Crossing Point

A novel, simple, accurate, sensitive, precise and economical derivative spectroscopic method was developed and validated for the determination of cefadroxil and probenecid in synthetic mixture. First order derivative spectroscopy method was adopted to eliminate spectral interference. The method obeys Beer’s Law in concentration ranges of 4-36 μg/ml for cefadroxil and of 5-25 μg/ml of probenecid. The zero crossing point for cefadroxil and probenecid was 260 nm and 237.8 nm respectively in 0.1N HCl. The method was validated in terms of accuracy, precision, linearity, limits of detection, limits of quantitation. This method has been successively applied to synthetic mixture and no interference from the synthetic mixture’s excipients was found.   

scholarly journals First-order derivative spectrophotometric estimation of gemifloxacin mesylate and ambroxol HCl in tablet dosage form

2021 ◽  
Vol 14 (2) ◽  
pp. 029-036
Author(s):  
Wrushali A. Panchale ◽  
Ravindra L. Bakal
Keyword(s):  
Dosage Form ◽  
Correlation Coefficients ◽  
Order Derivative ◽  
Simultaneous Estimation ◽  
Zero Crossing ◽  
First Order ◽  
Derivative Spectroscopy ◽  
Ich Guidelines ◽  
Tablet Dosage

Aim of present work was to develop and validate a simple, precise and accurate uv-vis spectrophotometric method for the simultaneous estimation of gemifloxacin mesylate (GEMI) and ambroxol HCl (AMB) in their combined tablet dosage form. The method is based on first-order derivative spectroscopy. For determination of sampling wavelengths, each of GEMI and AMB were scanned in the wavelength range of 200–400 nm in the spectrum mode and sampling wavelengths were selected at 360 nm (zero crossing of GEMI) where AMB showed considerable absorbance and at 221.6 nm (zero crossing of AMB) where GEMI showed considerable absorbance. The linearity was obtained in the concentration range of 32-192 µg/ml for GEMI and 7.5-45 µg/ml for AMB. The correlation coefficients were found to be 0.9987 and 0.9992 for GEMI and AMB, respectively. The method was validated as per ICH guidelines. Mean recoveries were found satisfactory. All the data of validation study was found to be satisfactorily. The proposed method can be applied for simultaneous estimation of both the drugs

scholarly journals A NEW VALIDATED THIRD ORDER DERIVATIVE SPECTROSCOPIC METHOD FOR SIMULTANEOUS ESTIMATION OF METOPROLOL SUCCINATE AND RAMIPRIL IN TABLET DOSAGE FORM

2020 ◽  
pp. 54-59
Author(s):  
ALEKHYA B. ◽  
M. SINDHUSHA ◽  
SORAJ K. RAUL ◽  
GOPAL K. PADHY
Keyword(s):  
Quantitative Analysis ◽  
Dosage Form ◽  
Spectroscopic Method ◽  
Simultaneous Estimation ◽  
Third Order ◽  
Metoprolol Succinate ◽  
Zero Crossing ◽  
Ich Guidelines ◽  
Tablet Dosage

Objective: The objective of the present work is to develop and validate a new UV derivative spectrophotometric method for simultaneous estimation of metoprolol succinate and ramipril in methanol: water (50:50v/v). Methods: “Zero crossing technique” was chosen for quantitative determination. The zero-crossing points (ZCP’s) were found to be 209 nm where metoprolol succinate was quantified and 211 nm where ramipril was quantified. This method was then subjected to accuracy, linearity, sensitivity and reproducibility according to ICH guidelines to ensure and confirm its validity. Results: The method was found to be obeying Beer’s law in the range of 10-50 µg/ml and 5-25 µg/ml for metoprolol succinate and ramipril, respectively. The % recoveries were observed between the range of 99.2-100.2 for metoprolol succinate and 99.57-99.86 for ramipril. The intra-day and inter-day results showed reproducibility. Conclusion: It can be concluded that the developed third-order UV derivative spectroscopic method for the simultaneous determination of metoprolol succinate and ramiprilcan be recommended for routine quantitative analysis.

scholarly journals Absorbance Correction Method for Simultaneous Estimation of Nifedipine and Metoprolol Succinate in Their Synthetic Mixture Using From Spectrophotometry

2015 ◽  
Vol 1 (3) ◽  
pp. 151
Author(s):  
Sojitra Rajanit ◽  
Paras Virani ◽  
Hashumati Raj
Keyword(s):  
Accurate Method ◽  
Correction Method ◽  
Synthetic Mixture ◽  
Simultaneous Estimation ◽  
Metoprolol Succinate ◽  
Absorption Correction ◽  
Ich Guidelines ◽  
Development And Validation ◽  
Tablet Dosage

A new simple, economical, precise and accurate method are described for the simultaneous determination of Nifedipine (NIF) and Metoprolol Succinate (MET) in combined tablet dosage form. The proposed method was applied for the determination of Nifedipine and Metoprolol Succinate in synthetic mixture, for determination of sampling wavelength, 10?g/ml of each of NIF and MET were scanned in 200-400 nm range and sampling wavelengths were 313nm for NIF and 275.40nm for MET are selected for development and validation of absorption correction method. For this method linearity observed in the range of 5-25?g/ml for NIF and 25-125?g/ml for MET, and in their pharmaceutical formulation with mean percentage recoveries 100.68 and 100.33, respectively. The method was validated according to ICH guidelines and can be applied for routine quality control testing.

scholarly journals Development and Validation of First-Order Derivative Spectrophotometry for Simultaneous Determination of Levocetirizine Dihydrochloride and Phenylephrine Hydrochloride in Pharmaceutical Dosage Form

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Kaminee Parmar ◽  
Sunil Baldania ◽  
Dimal Shah ◽  
Usmangani Chhalotiya ◽  
Naimin Parmar
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Correlation Coefficients ◽  
Order Derivative ◽  
Simultaneous Estimation ◽  
Pharmaceutical Dosage Form ◽  
Zero Crossing ◽  
Phenylephrine Hydrochloride ◽  
First Order ◽  
Crossing Point

A simple, precise, accurate, and economical spectrophotometric method has been developed for simultaneous estimation of levocetirizine dihydrochloride (LCT) and phenylephrine hydrochloride (PHE) by employing first-order derivative spectrophotometric method. The first-order derivative absorption at 240 nm (zero crossing point of PHE) was used for quantification of LCT and 283.2 nm (zero crossing point of LCT) for quantification of PHE. The linearity was established over the concentration range of 4–24 μg/mL and 8–48 μg/mL for LCT and PHE with correlation coefficients (r2) 0.9964 and 0.9972, respectively. The mean % recoveries were found to be in the range of 99.14%–100.43% for LCT and 98.73%–100.83% for PHE. The proposed method has been validated as per ICH guideline and successfully applied for the simultaneous estimation of LCT and PHE in combined tablet dosage form.

scholarly journals Spectrophotometric Simultaneous Determination of Salbutamol Sulfate and Ketotifen Fumarate in Combined Tablet Dosage Form by First-Order Derivative Spectroscopy Method

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Parth R. Joshi ◽  
Shraddha J. Parmar ◽  
Bhavna A. Patel
Keyword(s):  
Method Development ◽  
Order Derivative ◽  
Simultaneous Estimation ◽  
Salbutamol Sulfate ◽  
Pharmaceutical Dosage Forms ◽  
First Order ◽  
Ketotifen Fumarate ◽  
Derivative Spectroscopy ◽  
Relative Standard

Salbutamol sulfate and ketotifen fumarate are used in combination for the treatment of asthma. The present work deals with method development for simultaneous estimation of salbutamol sulfate and ketotifen fumarate in two-component tablet formulation by first-order derivative spectroscopy. For determination of sampling wavelength, 10 μg/mL of each of salbutamol and ketotifen was scanned in 200–400 nm ranges and sampling wavelengths were found to be 257 nm for salbutamol and 278 nm for ketotifen in first-order derivative spectroscopy. In this method, linearity was observed in the ranges of 5–45 μg/mL for salbutamol and 5–35 μg/mL for ketotifen. The % recovery was within the range between 98 and 102%, and % relative standard deviation for precision and accuracy of the method was found to be less than 2%. The method is validated as per international conference on harmonization guidelines. The method can be successfully applied for the simultaneous analysis of both drugs in pharmaceutical dosage forms.

scholarly journals Validation of a green spectrophotometric method for the determination of dropropizine in commercial oral solutions

2020 ◽  
Vol 4 (2) ◽  
pp. 12-18
Author(s):  
Millena Almeida Monsores ◽  
Mikaelly Pereira Caet ◽  
Anna Karolina Mouzer Machado ◽  
Marina Cardoso Nemitz ◽  
Vítor Todeschini ◽  
...  
Keyword(s):  
Spectrophotometric Method ◽  
Low Cost ◽  
Mean Value ◽  
Green Method ◽  
Zero Crossing ◽  
First Order ◽  
Relative Standard ◽  
Full Factorial ◽  
Crossing Point

The present work describes a first-order derivative spectrophotometric (1D-UV) method for determination of dropropizine in commercial oral solutions. The method was developed using ecologically correct solvents and validated according to International Conference on Harmonization (ICH) recommendations. The response was linear in the concentration range of 6–24 μg/mL (r = 0.9997, n = 7) at wavelength 249 nm, which was the zero crossing point of excipient solutions. The detection and quantitation limits were 0.36 and 1.18 μg/mL, respectively. The method showed adequate precision, with a relative standard deviation values lower than 1.41%. Excellent values of accuracy were obtained, with a mean value of 99.44%. The method proved to be robust by a full factorial design evaluation. It is simple, it has low cost, and it has low use of polluting reagents. Minimum environmental hazards observed and the results obtained attest to the reliability of the proposed green method, showing to be specific, linear, precise, accurate and robust. Thus, the validated 1D-UV spectrophotometric method was successfully applied to the quantitative analysis of dropropizine in oral solutions dosage forms, helping to improve quality control and to assure therapeutic efficacy.

scholarly journals Simultaneous Determination of Metoprolol-Hydrochlorothiazide and Propranolol-Hydrochlorothiazide in Combined Formulations by Derivative Spectroscopy

1997 ◽  
Vol 80 (2) ◽  
pp. 325-330 ◽  
Author(s):  
Challapalli V N Prasad ◽  
Vipin Bharadwaj ◽  
Vidya Narsimhan ◽  
Rama T Chowdhary ◽  
Pyare Parimoo
Keyword(s):  
Simultaneous Determination ◽  
Second Derivative ◽  
Zero Crossing ◽  
Derivative Spectra ◽  
Derivative Spectroscopy ◽  
Naoh Solution ◽  
Compensation Technique ◽  
Second Derivative Spectra ◽  
Crossing Point

Abstract A derivative spectrophotometric procedure was established for simultaneous determination of propranolol HCI (PP) with hydrochlorothiazide (HTZ) and metoprolol tartrate (MTP) with HTZ in tablet preparations. The method uses first- and second-derivative spectra of tablet extract in 0.01 N NaOH solution. Ratios of analyte concentrations in the mixture were determined by the compensation technique. The zero-crossing point (ZCP) was also used to estimate the amounts of PP and HTZ in the formulations, and results were compared with those from the compensation technique. The results were found to be precise and free from interferences.

scholarly journals Development and Validation of a Derivative Spectrophotometric Method for Simultaneous Determination of Simvastatin and Ezetimibe

2010 ◽  
Vol 7 (s1) ◽  
pp. S197-S202 ◽  
Author(s):  
Effat Souri ◽  
Masoud Amanlou
Keyword(s):  
Simultaneous Determination ◽  
Spectrophotometric Method ◽  
Dosage Form ◽  
Mechanisms Of Action ◽  
Dosage Forms ◽  
Pharmaceutical Dosage Form ◽  
Zero Crossing ◽  
First Order ◽  
Development And Validation

A combination of simvastatin and ezetimibe with complementary mechanisms of action is used for treating high levels of cholesterol in the blood. The aim of this study was to develop a rapid and sensitive derivative spectrophotometric method for analysis of these drugs in combined dosage forms. A first order derivative spectrophotometric method was developed for simultaneous determination of simvastatin and ezetimibe using zero-crossing technique. The measurements were carried out at 219 and 265 nm for simvastatin and ezetimibe respectively. The described method was found to be linear (r2>0.999) over the range of 2-40 μg/mL for simvastatin in the presence of 10 μg/mL ezetimibe at 219 nm and in the range of 1-20 μg/mL of ezetimibe in the presence of 20 μg/mL of simvastatin at 265 nm. The within-day and between-day precision values for both drugs were less than 3% (CV). Also, good recoveries were obtained with both synthetic mixtures and commercial tablets. The proposed method was successfully applied for simultaneous determination of simvastatin and ezetimibe in a pharmaceutical dosage form without any interference from excipients.

scholarly journals Dual Wavelength and First Order Derivative Spectrophotometric Methods for Simultaneous Estimation of Rivaroxaban and Aspirin in Synthetic Mixture

2020 ◽  
pp. 48-54
Author(s):  
Heba Zhdan ◽  
Nazira Sarkis
Keyword(s):  
Limit Of Detection ◽  
Wave Length ◽  
Dual Wavelength ◽  
Synthetic Mixture ◽  
Order Derivative ◽  
Simultaneous Estimation ◽  
Limit Of Quantification ◽  
Zero Crossing ◽  
First Order ◽  
Spectrophotometric Methods

Two simple, sensitive, accurate and precise spectrophotometric methods were developed and validated for simultaneous estimation of binary mixture of Rivaroxaban and Aspirin in their pure form and synthetic mixture in difficult ratio 1:20(Rivaroxaban: Aspirin). The first method is the dual wavelength method, where 250 nm and 286.44 nm were selected as λ1 and λ2 for determination. And the two wave length for determination of Aspirin were 243.53 nm and 259.2 nm. The linearity range was studied over concentration ranges (2 – 12) μg/mL and (40-240) μg/mL for Rivaroxaban and Aspirin respectively in both methods, with correlation coefficients not less than 0.9996 and up to 0.9999 respectively in a row. The limit of detection (LOD) and limit of quantification (LOQ) were 0.24μg/mL and 0.82 μg/mL for Rivaroxaban and for Aspirin were7.03 μg/mL and 22.01 μg/mL respectively in the first method. The second method is First order derivative. The wavelengths 275.25 nm and 250 nm were selected as zero crossing point to determine Rivaroxaban and Aspirin in a row. The LOD and LOQ were 0.31 μg/mL and 0.95 μg/mL for Rivaroxaban; and for Aspirin was found to be7.13 μg/Ml and 21.60μg/mL respectively. The two methods were successfully validated as per ICH guidelines. Both methods were simple, sensitive and rapid.

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