scholarly journals Behavioural and neurochemical characterisation of the anxiolytic properties of an aqueous extract of Dysphania ambrosioides (L.) Mosyakin and Clemants (Chenopodiaceae) in experimental mice

2021 ◽  
Vol 14 (03) ◽  
pp. 265-276
Author(s):  
Bigued ◽  
Germain Sotoing Taiwe ◽  
Jacqueline Stephanie Kameni Njapdounke ◽  
Joseph Ngaibi ◽  
Yvette Nguezeye ◽  
...  
Keyword(s):  
Open Field ◽  
Aqueous Extract ◽  
Mechanism Of Action ◽  
Elevated Plus Maze ◽  
Aqueous Extracts ◽  
Gaba Concentration ◽  
Elevated Plus Maze Test ◽  
Maze Test ◽  
Plus Maze ◽  
Brain Gaba

Ethnopharmacological relevance: Dysphania ambrosioides (L.) Mosyakin & Clemants (Chenopodiaceae) is a medicinal plant known for its anxiolytic, antidepressant and anticonvulsant activities in Cameroonian folk medicine. Aim of the study: The aim of this work is to evaluate the anxiolytic effects of Dysphania ambrosioides aqueous extracts and investigate its mechanism of action. Materials and methods: Elevated plus maze test and open field test were used for detecting it anxiolytic properties. The possible mechanism of action of the aqueous extracts were investigated after pretreatment of animals with different antagonists of GABAA complex receptors (5 mg/kg N-methyl-β-carboline-3-carboxamide, 4 mg/kg flumazenil or 2 mg/kg bicuculline) 30 minutes prior to the oral administration of 370 mg/kg Dysphania ambrosioides aqueous extract. Results: Dysphania ambrosioides increased the percentage of entries into and percentage of time in open arms, and reduced rearing, head dipping, and percentage of time in closed arms, in the elevated plus maze. It reduced rearing and defecation, and increased crossing, in the open field. In addition, anxiolytic-like properties of Dysphania ambrosioides were blocked by different antagonists of GABAA complex receptors (N-methyl-β-carboline-3-carboxamide, flumazenil or bicuculline) as examined in elevated plus maze test. Finally, the activity of GABA-T activity was inhibited and the brain GABA concentration was increased by the extracts, respectively. Conclusion: These results suggest that Dysphania ambrosioides possess anxiolytic-like properties in mice that might involve an action on benzodiazepine and/or GABA sites in the GABAA receptor complex or by modulating brain GABA concentration in the central nervous system.

scholarly journals Attenuation of anxiety on acute administration of aqueous extract of Terminalia belerica fruit pulp in Swiss albino mice

2017 ◽  
Vol 6 (2) ◽  
pp. 303
Author(s):  
Chandrashekar R. ◽  
Manohar V. R. ◽  
Poovizhi Bharathi R. ◽  
Mohandas Rai
Keyword(s):  
Aqueous Extract ◽  
Elevated Plus Maze ◽  
Acute Administration ◽  
Fruit Pulp ◽  
Swiss Albino Mice ◽  
Elevated Plus Maze Test ◽  
Maze Test ◽  
Plus Maze ◽  
Terminalia Belerica ◽  
Albino Mice

Background: The objective of this study was to evaluate the attenuation of anxiety on acute administration of aqueous extract of Terminalia belerica fruit pulp (AETBFP) by using elevated plus maze test and dark and light arena models.Methods: Thirty Swiss albino mice were divided into five groups, Group I received vehicle (1% Gum acacia suspension, 3ml/kg, orally), Group II received standard drug Diazepam (1mg/kg, orally) and Group III, IV and V received AETBFP 9, 18 and 36 mg/kg, orally respectively. In elevated plus maze test, the mouse was placed on the central platform facing towards open arm. The percentage of time spent and frequency of entries and number of rears in open arm was counted for a period of 5 min. In dark and light arena, the time spent, number of entries and number of rears in light arena was counted for a period of 5 min. The mean±SEM values were calculated for each group. The data was analyzed using one-way ANOVA followed by Dunnet’s multiple comparison tests; p< 0.05 was considered as statistically significant.Results: Significant (p<0.05) reduction in anxiety was noted in experimental animals when given at a dose of AETBFP (36mg/kg), where number of entries and duration of stay in open arm and light arena increased in elevated plus maze and light and dark arena respectively when compared with control animals.Conclusions: Our study reveals that AETBFP at a dose of 36mg/kg has significant attenuation of anxiety in Swiss albino mice.

scholarly journals The antianxiety activity of aqueous extract of Withania coagulans fruits in swiss albino mice by elevated plus maze

2020 ◽  
Vol 9 (9) ◽  
pp. 1337
Author(s):  
Devesh D. Gosavi ◽  
Amit S. Kamdi ◽  
Suvarna M. Kalambe ◽  
Pankaj N. Bohra
Keyword(s):  
Aqueous Extract ◽  
Open Space ◽  
Elevated Plus Maze ◽  
P Value ◽  
Swiss Albino Mice ◽  
Elevated Plus Maze Test ◽  
Maze Test ◽  
Withania Coagulans ◽  
Plus Maze ◽  
Albino Mice

Background: Several anti-anxiety drugs used today have the numerous side effects. The Withania coagulans a rare species, is not studied much for its actions on anxiety except in late seventies where it was explored for neuropsychopharmacology. Thus, it was thought commendable to explore the anti-anxiety activities of aqueous extract of Withania coagulans fruits in swiss albino mice using elevated plus maze test.Methods: Elevated plus maze (EPM) test was used for evaluating the anti-anxiety action of Withania coagulans fruits’ aqueous extract. If the extract had anti-anxiety action, then it was expected that the time spent in the open space would increase and that in the closed space would decrease. This increase in time in the open space, if found statistically significant, was considered for antianxiety action.Results: There was statistically highly significant (p value <0.001) association observed between the aqueous extract of Withania coagulans fruits with antianxiety action in the swiss albino mice by elevated plus maze test.Conclusions: The aqueous extract of Withania coagulans fruits did prove the antianxiety effect in the swiss albino mice by elevated plus maze test.

Anxiogenic-like effects ofUncariatomentosa(Willd.) DC. aqueous extract in an elevated plus maze test in mice: a preliminary study

2013 ◽  
Vol 27 (18) ◽  
pp. 1682-1685 ◽  
Author(s):  
María Celeste Bigliani ◽  
María Celeste Rosso ◽  
Paula M. Zunino ◽  
Gustavo Baiardi ◽  
Andrés Alberto Ponce
Keyword(s):  
Aqueous Extract ◽  
Elevated Plus Maze ◽  
Elevated Plus Maze Test ◽  
Maze Test ◽  
Plus Maze ◽  
Preliminary Study

scholarly journals A Novel Nutraceutical Formulation Can Improve Motor Activity and Decrease the Stress Level in a Murine Model of Middle-Age Animals

2021 ◽  
Vol 10 (4) ◽  
pp. 624
Author(s):  
Dimitris Tsoukalas ◽  
Ovidiu Zlatian ◽  
Mihaela Mitroi ◽  
Elisavet Renieri ◽  
Aristidis Tsatsakis ◽  
...  
Keyword(s):  
Open Field ◽  
Field Test ◽  
Elevated Plus Maze ◽  
Open Field Test ◽  
Centella Asiatica ◽  
The Elderly ◽  
Dependent Manner ◽  
Elevated Plus Maze Test ◽  
Maze Test ◽  
Plus Maze

Ageing is a genetically programmed physiological process that is modulated by numerous environmental factors, associated with decreasing physiological function, decreasing reproductive rate and increasing age-related mortality rate. Maintaining mobility performance and physical function in the elderly is the main objective of the successful ageing concept. In this study, we aimed to evaluate the beneficial effect of a novel nutraceutical formulation containing Centella asiatica L. extract, vitamin C, zinc and vitamin D3 (as cholecalciferol) on motor activity and anxiety with the use of a murine model of old animals, as a means of providing proof for clinical use in the elderly, for enhancing physical strength and improving life quality. Eighteen Sprague Dawley 18 months old male rats were divided into three groups and received corn oil (the control group) or 1 capsule/kg bw Reverse supplement (treatment group 1) or 2 capsules/kg bw Reverse supplement (treatment group 2), for a period of 3 months. The Reverse supplement (Natural Doctor S.A, Athens, Greece) contains 9 mg Centella asiatica L. extract, vitamin C (200 mg as magnesium ascorbate), zinc (5 mg as zinc citrate), vitamin D3 (50 µg as cholecalciferol) per capsule. Before and after the treatment, the motor function and behavioral changes for anxiety and depression were evaluated using the open-field test, elevated plus-maze test and rotarod test. The supplementation with Reverse (Natural Doctor S.A) supplement can improve the locomotor activity in old rats in a dose-dependent manner, as demonstrated by an increase in the latency to leave from the middle square, in the number of rearings in the open field test, in the time spent in the open arms and time spent in the center in the elevated plus-maze test and the latency to all in all three consecutive trials in the rotarod test. Stress also decreased significantly in a dose-dependent manner, following the treatment with Reverse supplement, as was demonstrated by the decrease in the number of groomings at the open field test and time spent in the dark and the number of groomings at the elevated plus-maze test.

scholarly journals GPR30 activation decreases anxiety in the open field test but not in the elevated plus maze test in female mice

2013 ◽  
Vol 4 (1) ◽  
pp. 51-59 ◽  
Author(s):  
Divya Anchan ◽  
Sara Clark ◽  
Kevin Pollard ◽  
Nandini Vasudevan
Keyword(s):  
Open Field ◽  
Field Test ◽  
Elevated Plus Maze ◽  
Open Field Test ◽  
Female Mice ◽  
Elevated Plus Maze Test ◽  
Maze Test ◽  
Plus Maze

scholarly journals Anxiolytic and antipyretic properties of the root aqueous extract of Bombax costatum Pellegr. et Vuillet. (Bombacaceae) in mice: Implication of behavioural and neurochemical approaches

2021 ◽  
Vol 15 (2) ◽  
pp. 140-150
Author(s):  
Ngassia Wanbara ◽  
Germain Sotoing Taiwe ◽  
Jacqueline Stephanie Kameni Njapdounke ◽  
Neteydji Sidiki ◽  
Alexandre Michel Njan Nloga ◽  
...  
Keyword(s):  
Traditional Medicine ◽  
Aqueous Extract ◽  
Plant Extract ◽  
Elevated Plus Maze ◽  
The Body ◽  
Control Group ◽  
Induced Hyperthermia ◽  
Elevated Plus Maze Test ◽  
Maze Test ◽  
Plus Maze

Ethnopharmacological relevance: Bombax costatum Pellegr. et Vuillet. (Bombacaceaea) is a plant used in traditional medicine in Cameroon to treat memory impairment, anxiety, insomnia and agitation. Aim of the study: The aqueous extract of Bombax costatum is evaluated for its anxiolytic like effect in mice using experimental models. Materials and methods: The plant extract is administered orally to mice. They were tested one hour later in the stress-induced hyperthermia, hole board, and open field or elevated plus maze tests, respectively. Finally, the brain Gamma aminobutyric acid [GABA] content and GABA-T were quantified in Bombax costatum aqueous extract-treated mice at the end of elevated plus maze test. Results: Bombax costatum aqueous extract showed anxiolytic activity. In stress-induced hyperthermia test, the plant extract significantly antagonised the increase of temperature. There is a significant reduction in the stress-induced hyperthermia from 1.13 ± 0.06℃ in the negative control group treated with distilled water to 0.26 ± 0.02℃ in the group of mice administered 100 mg/kg aqueous extract. In addition, Bombax costatum showed antipyretic activity by reducing the body temperature. In the elevated plus maze test, the aqueous extract increased the number of entries into, percentage of entries into, and percentage of time in open arms. It also reduced the percentage of entries and time in closed arms. Conclusion: The obtained results suggested that Bombax costatum aqueous extracts possess anxiolytic-like and antipyretic activities in mice. This plant could be helpful in the treatment of anxiety and fever in traditional medicine in Cameroon.

Sex differences in the elevated plus-maze test and large open field test in adult Wistar rats

2021 ◽  
Vol 204 ◽  
pp. 173168
Author(s):  
Parker Knight ◽  
Ranjithkumar Chellian ◽  
Ryann Wilson ◽  
Azin Behnood-Rod ◽  
Stefany Panunzio ◽  
...  
Keyword(s):  
Sex Differences ◽  
Open Field ◽  
Field Test ◽  
Wistar Rats ◽  
Elevated Plus Maze ◽  
Open Field Test ◽  
Elevated Plus Maze Test ◽  
Maze Test ◽  
Plus Maze

Anticonvulsant, Anxiolytic and Antidepressant Properties of the β-caryophyllene in Swiss Mice: Involvement of Benzodiazepine-GABAAergic, Serotonergic and Nitrergic Systems

2020 ◽  
Vol 14 (1) ◽  
pp. 36-51 ◽  
Author(s):  
George L. da Silva Oliveira ◽  
José C. Correia L. da Silva ◽  
Ana P. dos Santos C. L da Silva ◽  
Chistiane M. Feitosa ◽  
Fernanda R. de Castro Almeida
Keyword(s):  
Receptor Antagonist ◽  
Molecular Mechanisms ◽  
Elevated Plus Maze ◽  
Feeding Test ◽  
Swiss Mice ◽  
Elevated Plus Maze Test ◽  
Maze Test ◽  
Plus Maze ◽  
Gabaergic Receptors ◽  
Pre Treatment

Background: Central nervous system disorders such as anxiety, depression and epilepsy are characterized by sharing several molecular mechanisms in common and the involvement of the L-arginine/NO pathway in neurobehavioral studies with β-caryophyllene is still little discussed. Objectives: One of the objectives of the present study was to demonstrate the anxiolytic behavioral effect of β-caryophyllene (β-CBP) in female Swiss mice, as well as to investigate the molecular mechanisms underlying the results obtained. Methods: This study evaluated the neurobehavioral effects of β-CBP using the open field test, rota-rod test, elevated plus maze test, novelty suppressed feeding test, tail suspension test and forced swim test, as well as pilocarpine, pentylenetetrazole and isoniazid-induced epileptic seizure models. Results:: The results demonstrated that the neuropharmacological activities of β-CBP may involve benzodiazepine/GABAergic receptors, since the pre-treatment of β-CBP (200 mg/kg) associated with flumazenil (5 mg/kg, benzodiazepine receptor antagonist) and bicuculline (1 mg/kg, selective GABAA receptor antagonist) reestablished the anxiety parameters in the elevated plus-maze test, as well as the results of reduced latency to consume food in the novelty suppressed feeding test. In addition to benzodiazepine/GABAergic receptors, the neuropharmacological properties of β-CBP may be related to inhibition of nitric oxide synthesis, since pre-treatment with L-arginine (500- 750 mg/kg) reversed significantly the anxiolytic, antidepressant and anticonvulsant activities of β-CBP. Conclusion: The results obtained provide additional support in understanding the neuromolecular mechanisms underlying the anxiolytic, antidepressant and anticonvulsive properties of β-CBP in female Swiss mice.

scholarly journals Effects of test experience, closed-arm wall color, and illumination level on behavior and plasma corticosterone response in an elevated plus maze in male C57BL/6J mice: a challenge against conventional interpretation of the test

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Hirotaka Shoji ◽  
Tsuyoshi Miyakawa
Keyword(s):  
Elevated Plus Maze ◽  
Plasma Corticosterone ◽  
Illumination Level ◽  
Test Battery ◽  
Lower Percentage ◽  
Behavioral Tests ◽  
Elevated Plus Maze Test ◽  
Maze Test ◽  
Plus Maze ◽  
Corticosterone Response

AbstractThe elevated plus maze test is a widely used test for assessing anxiety-like behavior and screening novel therapeutic agents in rodents. Previous studies have shown that a variety of internal factors and procedural variables can influence elevated plus maze behavior. Although some studies have suggested a link between behavior and plasma corticosterone levels, the relationships between them remain unclear. In this study, we investigated the effects of experience with a battery of behavioral tests, the wall color of the closed arms, and illumination level on the behavior and plasma corticosterone responses in the elevated plus maze in male C57BL/6J mice. Mice were either subjected to a series of behavioral tests, including assessments of general health and neurological function, a light/dark transition test, and an open field test, or left undisturbed until the start of the elevated plus maze test. The mice with and without test battery experience were allowed to freely explore the elevated plus maze. The other two independent groups of naïve mice were tested in mazes with closed arms with different wall colors (clear, transparent blue, white, and black) or different illumination levels (5, 100, and 800 lx). Immediately after the test, blood was collected to measure plasma corticosterone concentrations. Mice with test battery experience showed a lower percentage of open arm time and entries and, somewhat paradoxically, had lower plasma corticosterone levels than the mice with no test battery experience. Mice tested in the maze with closed arms with clear walls exhibited higher open arm exploration than mice tested in the maze with closed arms with black walls, while there were no significant differences in plasma corticosterone levels between the different wall color conditions. Illumination levels had no significant effects on any measure. Our results indicate that experience with other behavioral tests and different physical features of the maze affect elevated plus maze behaviors. Increased open arm time and entries are conventionally interpreted as decreased anxiety-like behavior, while other possible interpretations are considered: open arm exploration may reflect heightened anxiety and panic-like reaction to a novel situation under certain conditions. With the possibility of different interpretations, the present findings highlight the need to carefully consider the test conditions in designing experiments and drawing conclusions from the behavioral outcomes in the elevated plus maze test in C57BL/6J mice.

Sign in / Sign up

Export Citation Format

Share Document