scholarly journals Liver cell membrane antibody, kidney injury molecule and lungs tumour antigen expression on the MCF-7 cell induced breast cancer administered with Citrus limon juice and tamoxifen in Sprawgue dawley rats

2020 ◽  
Vol 8 (3) ◽  
pp. 293-301
Author(s):  
Oyebadejo SA ◽  
Nsuhoridem SA ◽  
Oyeleye OE
Keyword(s):  
Breast Cancer ◽  
Cell Membrane ◽  
Cell Line ◽  
Liver Cell ◽  
Kidney Injury ◽  
Antigen Expression ◽  
Monitoring And Evaluation ◽  
Citrus Limon ◽  
Liver Cell Membrane ◽  
Mcf 7

Monitoring and evaluation have been one of the major measures to determine possible progression of response of disorder to the alternative treatments, however Anti-Liver Cell membrane Antibody, Kidney Injury Molecule and Lungs Tumour Antigen Expression on the MCF-7 Cell Induced Breast Cancer Rats administered with Citrus Limon juice and Tamoxifen were carried out, Over one hundred and twenty Sprague dawley rats of 40 days old average body weight 180-220g were divided into ten (10) containing of 12 animals per group, group 1 was control, fed only with rat chow and water, group 2 was MCF-7 cell line induced rats alone (BCIR only), group 3 Citrus Limon juice (CLJ) at 8.88%, Group 4 Citrus Limon juice (CLJ) at 17.32%, group 5 Citrus Limon juice (CLJ) at 25.98%, group 6 was given 0.2mg/kg of Tamoxifen alone, group 7 (BCIR+CLJ at 8.88%), group 8 (BCIR+CLJ at 17.32%), group 9 (BCIR+CLJ at 25.98%) and group (BCIR+ 0.2mg/kg of Tamoxifen). Acute and sub – acute toxicity were carried out after the establishment of safety dose following determination of LD50, on the fresh Citrus Limon juice Breast cancer induction and Tumor sizes were analysed. At the end of the administration, animals were sacrificed, fresh blood was taken, centrifuged at 3000 rpm, serum was collected and stored at 8oc Breast Cancer Tumor Associated Assays, Kidney Injury, Liver cell Membrane Antibody and Lung Tumor Antigens were analysed. Result, Mammary tumor induction was succeeded in all the MCF-7 cell line induced groups with minimum number of morbidity. Tumor size was significantly increase in tumor mass BCIR only at P>0.001 when compared to BCIR + CLJ and BCIR + Tamoxifen groups. Immunoogical expression of Breast Cancer Associated Tumor revealed significant increase suppressor gene/protein, Immunocytochemical in-vivo of Breast Cancer Associated Organs showed significant increase in KIM , LCMA and LTA at P>0.001 in BCIR when compared to all the BCIR + CLJ, BCIR + Tamoxifen and other non-BCIR groups. In conclusion, this study showed that KIM , LCMA and LTA exhibited strong Immunochemical expression of Citrus Limon juice potency possessing possible anticancer activities without posing pathological conditions on the body during and after usage, hence KIM, LCMA and LTA are demonstrated to be valuable in the monitoring and evaluation progression of tumor as Citrus Limon Juice could be used as alternative therapy in the treatment of Breast cancer that are hormonal dependant in similar manner to Tamoxifen.

scholarly journals 57. A NEW METHOD FOR DETECTING ANTI LIVER CELL MEMBRANE ANTIBODY USING A HUMAN HEPATOMA CELL LINE

1987 ◽  
Vol 22 (1) ◽  
pp. 105-105
Author(s):  
A Lobo-Yeo CMcSorley ◽  
I M McFarlane ◽  
A P Mowat ◽  
G Mielivergani ◽  
D Vergani
Keyword(s):  
Cell Membrane ◽  
Cell Line ◽  
Liver Cell ◽  
Hepatoma Cell ◽  
New Method ◽  
Hepatoma Cell Line ◽  
Human Hepatoma ◽  
Human Hepatoma Cell ◽  
Human Hepatoma Cell Line ◽  
Liver Cell Membrane

A Novel Triazole Derivative Drug Presenting In Vitro and In Vivo Anticancer Properties

2018 ◽  
Vol 18 (17) ◽  
pp. 1483-1493
Author(s):  
Ricardo Imbroisi Filho ◽  
Daniel T.G. Gonzaga ◽  
Thainá M. Demaria ◽  
João G.B. Leandro ◽  
Dora C.S. Costa ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Hepatic Function ◽  
Anticancer Properties ◽  
Mcf 7

Background: Cancer is a major cause of death worldwide, despite many different drugs available to treat the disease. This high mortality rate is largely due to the complexity of the disease, which results from several genetic and epigenetic changes. Therefore, researchers are constantly searching for novel drugs that can target different and multiple aspects of cancer. Experimental: After a screening, we selected one novel molecule, out of ninety-four triazole derivatives, that strongly affects the viability and proliferation of the human breast cancer cell line MCF-7, with minimal effects on non-cancer cells. The drug, named DAN94, induced a dose-dependent decrease in MCF-7 cells viability, with an IC50 of 3.2 ± 0.2 µM. Additionally, DAN94 interfered with mitochondria metabolism promoting reactive oxygen species production, triggering apoptosis and arresting the cancer cells on G1/G0 phase of cell cycle, inhibiting cell proliferation. These effects are not observed when the drug was tested in the non-cancer cell line MCF10A. Using a mouse model with xenograft tumor implants, the drug preventing tumor growth presented no toxicity for the animal and without altering biochemical markers of hepatic function. Results and Conclusion: The novel drug DAN94 is selective for cancer cells, targeting the mitochondrial metabolism, which culminates in the cancer cell death. In the end, DAN94 has been shown to be a promising drug for controlling breast cancer with minimal undesirable effects.

Design, Synthesis and Cytotoxicity Evaluation of New 3, 5-Disubstituted-2-Thioxoimidazolidinones

2018 ◽  
Vol 18 (4) ◽  
pp. 573-582 ◽  
Author(s):  
Khaled R.A. Abdellatif ◽  
Mostafa M. Elbadawi ◽  
Mohammed T. Elsaady ◽  
Amer A. Abd El-Hafeez ◽  
Takashi Fujimura ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Topoisomerase I ◽  
Cancer Cell Line ◽  
Cytotoxic Agents ◽  
Dependent Manner ◽  
Human Lung Fibroblast ◽  
Mcf 7

Background: Some 2-thioxoimidazolidinones have been reported as anti-prostate and anti-breast cancer agents through their inhibitory activity on topoisomerase I that is considered as a potential chemotherapeutic target. Objective: A new series of 3,5-disubstituted-2-thioxoimidazolidinone derivatives 10a-f and their S-methyl analogs 11a-f were designed, synthesized and evaluated for cytotoxicity against human prostate cancer cell line (PC-3), human breast cancer cell line (MCF-7) and non-cancerous human lung fibroblast cell line (WI-38). </P><P> Results and Method: While compounds 10a-f showed a broad range of activities against PC-3 and MCF-7 cell lines (IC50 = 34.0 – 186.9 and 24.6 – 147.5 µM respectively), the S-methyl analogs 11a-f showed (IC50 = 22.7 – 198.5 and 16.9 – 188.2 µM respectively) in comparison with 5-fluorouracil (IC50 = 60.7 and 40.7 µM respectively). 11c (IC50 = 22.7 and 29.2 µM) and 11f (IC50 = 28.7 and 16.9 µM) were the most potent among all compounds against both PC-3 and MCF-7 respectively with no cytotoxicity against WI-38. Conclusion: The newly synthesized compounds showed good activity against PC-3 and MCF-7 cell lines in comparison with 5-fluorouracil. Compounds 11c and 11f bound with human topoisomerase I similar to its known inhibitors and significantly inhibited its DNA relaxation activity in a dose dependent manner which may rationalize their molecular mechanism as cytotoxic agents.

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