Biological synthesis and characterization of copper oxide nanoparticles using aqueous Psidium guajava leave extract and study of antibacterial activity of the copper oxide nanoparticles on Escherichia coli and Staphylococcus aureus

Nanobiotechnogy has been developed to synthesized copper oxide nanoparticle. This was carried out biologically using leaf extract of Psidium guajava as a reducing and stabilizing agent. Optical property showed a color change from dark brown to gold- yellow after the addition of leaf extract. The possible functional group in the plant extract responsible for the reduction of CuONps was identified as belonging to alkyl halide group by FT-IR analysis. UV- visible spectra of copper oxide nanoparticle showed a surface plasmon resonance peak at 390nm. The biologically synthesized copper nanoparticles were tested for antibacterial activity against two human pathogens viz, Escherichia coli and Staphylococcus aureus. Biosynthesized copper nanoparticle showed clear zone of inhibition or better activity against Escherichia coli and Staphylococcus aureus.

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Green Synthesis and Characterization of Copper Oxide Nanoparticles Using Psidium guajava Leaf Extract

IOP Conference Series Materials Science and Engineering ◽

10.1088/1757-899x/961/1/012011 ◽

2020 ◽

Vol 961 ◽

pp. 012011

Author(s):

Ammu Varughese ◽

Raminder Kaur ◽

Poonam Singh

Keyword(s):

Copper Oxide ◽

Green Synthesis ◽

Leaf Extract ◽

Psidium Guajava ◽

Copper Oxide Nanoparticles ◽

Synthesis And Characterization ◽

Oxide Nanoparticles

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Bacterial Synthesis of Copper/Copper Oxide Nanoparticles

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2008.095 ◽

2008 ◽

Vol 8 (6) ◽

pp. 3191-3196 ◽

Cited By ~ 65

Author(s):

Syed Saif Hasan ◽

Sanjay Singh ◽

Rasesh Y. Parikh ◽

Mahesh S. Dharne ◽

Milind S. Patole ◽

...

Keyword(s):

Copper Oxide ◽

Copper Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Bacterial Cells ◽

Negative Bacterium ◽

Gram Negative ◽

Nanoparticle Formation ◽

Copper Oxide Nanoparticle ◽

Ftir Studies ◽

Oxide Nanoparticle

A bacterial mediated synthesis of copper/copper oxide nanoparticle composite is reported. A Gram-negative bacterium belonging to the genus Serratia was isolated from the midgut of Stibara sp., an insect of the Cerambycidae family of beetles found in the Northwestern Ghats of India. This is a unique bacterium that is quite specific for the synthesis of copper oxide nanoparticles as several other strains isolated from the same insect and common Indian mosquitoes did not result in nanoparticle formation. By following the reaction systematically, we could delineate that the nanoparticle formation occurs intracellularly. However, the process results in the killing of bacterial cells. Subsequently the nanoparticles leak out as the cell wall disintegrates. The nanoparticles formed are thoroughly characterized by UV-Vis, TEM, XRD, XPS and FTIR studies.

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Co-Treatment of Copper Oxide Nanoparticle and Carbofuran Enhances Cardiotoxicity in Zebrafish Embryos

International Journal of Molecular Sciences ◽

10.3390/ijms22158259 ◽

2021 ◽

Vol 22 (15) ◽

pp. 8259

Author(s):

Ferry Saputra ◽

Boontida Uapipatanakul ◽

Jiann-Shing Lee ◽

Shih-Min Hung ◽

Jong-Chin Huang ◽

...

Keyword(s):

Copper Oxide ◽

Food Production ◽

Copper Oxide Nanoparticles ◽

Human Consumption ◽

Oxide Nanoparticles ◽

Aquatic Environments ◽

Copper Oxide Nanoparticle ◽

Zebrafish Larvae ◽

Oxide Nanoparticle ◽

Carbamate Pesticide

The use of chemicals to boost food production increases as human consumption also increases. The insectidal, nematicidal and acaricidal chemical carbofuran (CAF), is among the highly toxic carbamate pesticide used today. Alongside, copper oxide nanoparticles (CuO) are also used as pesticides due to their broad-spectrum antimicrobial activity. The overuse of these pesticides may lead to leaching into the aquatic environments and could potentially cause adverse effects to aquatic animals. The aim of this study is to assess the effects of carbofuran and copper oxide nanoparticles into the cardiovascular system of zebrafish and unveil the mechanism behind them. We found that a combination of copper oxide nanoparticle and carbofuran increases cardiac edema in zebrafish larvae and disturbs cardiac rhythm of zebrafish. Furthermore, molecular docking data show that carbofuran inhibits acetylcholinesterase (AChE) activity in silico, thus leading to impair cardiac rhythms. Overall, our data suggest that copper oxide nanoparticle and carbofuran combinations work synergistically to enhance toxicity on the cardiovascular performance of zebrafish larvae.

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