Bacterial Synthesis of Copper/Copper Oxide Nanoparticles

2008 ◽  
Vol 8 (6) ◽  
pp. 3191-3196 ◽  
Author(s):  
Syed Saif Hasan ◽  
Sanjay Singh ◽  
Rasesh Y. Parikh ◽  
Mahesh S. Dharne ◽  
Milind S. Patole ◽  
...  
Keyword(s):  
Copper Oxide ◽  
Copper Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Bacterial Cells ◽  
Negative Bacterium ◽  
Gram Negative ◽  
Nanoparticle Formation ◽  
Copper Oxide Nanoparticle ◽  
Ftir Studies ◽  
Oxide Nanoparticle

A bacterial mediated synthesis of copper/copper oxide nanoparticle composite is reported. A Gram-negative bacterium belonging to the genus Serratia was isolated from the midgut of Stibara sp., an insect of the Cerambycidae family of beetles found in the Northwestern Ghats of India. This is a unique bacterium that is quite specific for the synthesis of copper oxide nanoparticles as several other strains isolated from the same insect and common Indian mosquitoes did not result in nanoparticle formation. By following the reaction systematically, we could delineate that the nanoparticle formation occurs intracellularly. However, the process results in the killing of bacterial cells. Subsequently the nanoparticles leak out as the cell wall disintegrates. The nanoparticles formed are thoroughly characterized by UV-Vis, TEM, XRD, XPS and FTIR studies.

scholarly journals Co-Treatment of Copper Oxide Nanoparticle and Carbofuran Enhances Cardiotoxicity in Zebrafish Embryos

2021 ◽  
Vol 22 (15) ◽  
pp. 8259
Author(s):  
Ferry Saputra ◽  
Boontida Uapipatanakul ◽  
Jiann-Shing Lee ◽  
Shih-Min Hung ◽  
Jong-Chin Huang ◽  
...  
Keyword(s):  
Copper Oxide ◽  
Food Production ◽  
Copper Oxide Nanoparticles ◽  
Human Consumption ◽  
Oxide Nanoparticles ◽  
Aquatic Environments ◽  
Copper Oxide Nanoparticle ◽  
Zebrafish Larvae ◽  
Oxide Nanoparticle ◽  
Carbamate Pesticide

The use of chemicals to boost food production increases as human consumption also increases. The insectidal, nematicidal and acaricidal chemical carbofuran (CAF), is among the highly toxic carbamate pesticide used today. Alongside, copper oxide nanoparticles (CuO) are also used as pesticides due to their broad-spectrum antimicrobial activity. The overuse of these pesticides may lead to leaching into the aquatic environments and could potentially cause adverse effects to aquatic animals. The aim of this study is to assess the effects of carbofuran and copper oxide nanoparticles into the cardiovascular system of zebrafish and unveil the mechanism behind them. We found that a combination of copper oxide nanoparticle and carbofuran increases cardiac edema in zebrafish larvae and disturbs cardiac rhythm of zebrafish. Furthermore, molecular docking data show that carbofuran inhibits acetylcholinesterase (AChE) activity in silico, thus leading to impair cardiac rhythms. Overall, our data suggest that copper oxide nanoparticle and carbofuran combinations work synergistically to enhance toxicity on the cardiovascular performance of zebrafish larvae.

scholarly journals Biological synthesis and characterization of copper oxide nanoparticles using aqueous Psidium guajava leave extract and study of antibacterial activity of the copper oxide nanoparticles on Escherichia coli and Staphylococcus aureus

2021 ◽  
Vol 9 (1) ◽  
pp. 114-120
Author(s):  
Samuel Paul ◽  
Akintunde Sheyi ◽  
Iliya Daniel Bangu ◽  
Sani Idris Abubakar ◽  
Muawiyya Muazu Muhammad
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Copper Oxide ◽  
Leaf Extract ◽  
Psidium Guajava ◽  
Copper Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Copper Oxide Nanoparticle ◽  
Oxide Nanoparticle ◽  
And Staphylococcus Aureus

Nanobiotechnogy has been developed to synthesized copper oxide nanoparticle. This was carried out biologically using leaf extract of Psidium guajava as a reducing and stabilizing agent. Optical property showed a color change from dark brown to gold- yellow after the addition of leaf extract. The possible functional group in the plant extract responsible for the reduction of CuONps was identified as belonging to alkyl halide group by FT-IR analysis. UV- visible spectra of copper oxide nanoparticle showed a surface plasmon resonance peak at 390nm. The biologically synthesized copper nanoparticles were tested for antibacterial activity against two human pathogens viz, Escherichia coli and Staphylococcus aureus. Biosynthesized copper nanoparticle showed clear zone of inhibition or better activity against Escherichia coli and Staphylococcus aureus.

Biosynthesis of Copper Oxide Nanoparticles Using Lactobacillus casei Subsp. Casei and its Anticancer and Antibacterial Activities

2020 ◽  
Vol 16 (1) ◽  
pp. 101-111 ◽  
Author(s):  
Mehri Kouhkan ◽  
Parinaz Ahangar ◽  
Leila Ashrafi Babaganjeh ◽  
Maryam Allahyari-Devin
Keyword(s):  
Copper Oxide ◽  
Cancer Cells ◽  
Lactobacillus Casei ◽  
Copper Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
No Production ◽  
Cytotoxic Effects ◽  
Gram Negative ◽  
X Ray ◽  
Cuo Nps

Background:The present study reveals the synthesis of copper oxide nanoparticles (CuO NPs) by probiotic bacteria (Lactobacillus casei subsp. casei) and demonstrates the cytotoxic effects of these nanoparticles against gram negative and positive bacteria and cancer cell lines.Methods:The CuO NPs are biosynthesized from Lactobacillus casei subsp. casei (L. casei) in an eco-friendly and cost-effective process. These nanoparticles are characterized using Fourier transform infrared spectroscopy (FT-IR), X-ray diffractometer (XRD), field emission scanning electron microscopy (FESEM), energy dispersive X-ray (EDX) and transmittance electron microscope (TEM) analysis. The antibacterial activity is examined by Well-diffusion, minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) assays using Broth microdilution. Anticancer effects of these nanoparticles are evaluated by methyl thiazolyl diphenyl-tetrazolium bromide (MTT) assay and Griess test.Results:Our results confirm the biosynthesis of CuO NPs from L. casei. Antibacterial assays demonstrate that treatment of gram-negative and gram-positive bacteria with CuO NPs inhibits the growth of these bacteria. Furthermore, the cell viability of human cancer cells decreases while treated by nanoparticles. These nanoparticles increase nitric oxide (NO) secretion determined by NO production measurement.Conclusion:These results suggest that CuO NPs may exert antibacterial effects as well as cytotoxic effects on cancer cells by suppressing their growth, increasing the oxidative stress and inducing apoptosis.

ANTIBIOTIC PROFILE AND ANTIBACTERIAL ACTIVITY OF COPPER OXIDE NANOPARTICLES AGAINST Pseudomonas fluorescens ISOLATED FROM WOUND INFECTION

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Haider Qassim Raheem ◽  
Takwa S. Al-meamar ◽  
Anas M. Almamoori
Keyword(s):  
Antibacterial Activity ◽  
Copper Oxide ◽  
Pseudomonas Fluorescens ◽  
Wide Spectrum ◽  
Copper Oxide Nanoparticles ◽  
Morphological Properties ◽  
Clinical Samples ◽  
Oxide Nanoparticles ◽  
Cuo Nps ◽  
Disk Diffusion Assay

Fifty specimens were collected from wound patients who visited Al-Hilla Teaching Hospital. The samples were grown on Blood and MacConkey agar for 24-48 hr at 37oC. The bacterial isolates which achieved as a pure and predominant growth from clinical samples as Pseudomonas fluorescens, were identified using morphological properties and Vitek2 system. The anti-bacterial activity of copper oxide nanoparticles (CuO NPs) against was tested by (disk diffusion assay) using dilutions of (400, 200, 100, 50, 25, and 12.5‎µ‎g/ml). The (MIC and MBC) of each isolate was determined. CuO NPs shows wide spectrum antibacterial activity against tested bacteria with rise zone of inhibition diameter that is proportionate with the increase in nanoparticle concentration. The MIC of CuO NPs extended from 100-200‎µ‎g/ml and the MBC ranged from 200-400‎µ‎g/ml. The antibiotic profile was determined by Viteck 2 compact system (Biomérieux). CuO NPs‎ found highly effective and safe in P. fluorescens wounds infections comparing with used antibiotics.

Green synthesis of copper oxide nanoparticles using aloe vera extract

2020 ◽  
Vol 3 (3) ◽  
Author(s):  
Hemalatha D ◽  
Saraswath S
Keyword(s):  
Copper Oxide ◽  
Biomedical Application ◽  
Aloe Vera ◽  
Copper Oxide Nanoparticles ◽  
Klebsiella Pneumonia ◽  
Oxide Nanoparticles ◽  
Cupric Sulfate ◽  
X Ray Diffraction ◽  
Fouriertransform Infrared Spectroscopy ◽  
Effective Use

In material science, green method for synthesis of nanomaterials is feasible, cheaper and eco-friendly protocol. To accomplish this phenomenon, present study was aimed to synthesize Copper oxide nanoparticles using leaf extract of Aloevera with two different precursors CuCl2.2H2O (Cupric chloride) and CuSo4.5H2O (Cupric sulfate). The extraction of Aloevera is employed as reducing and stabilizing agent for this synthesis.Copper oxide Nanoparticles is effective use of biomedical application due to their antibacterial function. The synthesized Copper oxide nanoparticles were characterized by X-Ray Diffraction Spectroscopy (XRD), Energy Dispersive Spectroscopy (EDX), FourierTransform Infrared Spectroscopy (FT- IR) and Scanning Electron Microscope(SEM). XRD studies reveal the crystallographic nature of Copper oxide nanoparticles. Furthermore the Copper oxide nanoparticles have good Antibacterial activity against both gram negative (E.Coli, Klebsiella pneumonia) and gram positive (Bacillus cereus, Staphylococcus aureus)bacteria.

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