scholarly journals Molecular Characterization of Trichophyton Verrucosum Strains İsolated From Cattle by PCR-RFLP

2021 ◽  
Author(s):  
Osman Yaşar TEL ◽  
Ayfer GÜLLÜ YÜCETEPE ◽  
Oktay KESKİN ◽  
Sevil ERDENLİĞ GÜRBİLEK
Keyword(s):  
Molecular Characterization ◽  
Trichophyton Verrucosum ◽  
Pcr Rflp

scholarly journals Molecular Characterization of Pathogenicity Locus (PaLoc) and tcdC Genetic Diversity Among tcdA+B+ Clostridioides Difficile Clinical Isolates in Tehran, Iran

2020 ◽  
Author(s):  
Mansoor Kodori ◽  
Zohreh Ghalavand ◽  
Abbas Yadegar ◽  
Gita Eslami ◽  
Masoumeh Azimirad ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Characterization ◽  
Disease Severity ◽  
Genetic Variants ◽  
Rflp Analysis ◽  
Clostridioides Difficile ◽  
Pcr Rflp ◽  
The Common ◽  
Healthcare Associated

Abstract Background: Clostridioides difficile is the main cause of healthcare-associated diarrhea worldwide. It is proposed that certain C. difficile toxinotypes with distinct pathogenicity locus (PaLoc) variants are associated with disease severity and outcomes. Additionally, few studies have described the common C. difficile toxinotypes, and also little is known about the tcdC variants in Iranian isolates. We characterized the toxinotypes and the tcdC genotypes from a collection of Iranian clinical C. difficile tcdA+B+ isolates with known ribotypes (RTs).Methods: Fifty C. difficile isolates with known RTs and carrying the tcdA and tcdB toxin genes were analyzed. Toxinotyping was carried out based on a PCR-RFLP analysis of a 19.6 kb region encompassing the PaLoc. Genetic diversity of the tcdC gene was determined by the sequencing of the gene.Results: Of the 50 C. difficile isolates investigated, five distinct toxinotypes were recognized. Toxinotypes 0 (33/50, 66%) and V (11/50, 22%) were the most frequently found. C. difficile isolates of the toxinotype 0 mostly belonged to RT 001 (12/33, 36.4%), whereas toxinotype V consisted of RT 126 (9/11, 81.8%). The tcdC sequencing showed six variants (35/50, 70%); tcdC-sc3 (24%), tcdC-A (22%), tcdC-sc9 (18%), tcdC-B (2%), tcdC-sc14 (2%), and tcdC-sc15 (2%). The remaining isolates were wild-types (15/50, 30%) in the tcdC gene.Conclusions: The present study demonstrates that the majority of clinical tcdA+B+ isolates of C. difficile frequently harbor tcdC genetic variants. We also found that the RT 001/ toxinotype 0 and the RT 126/ toxinotype V are the most common types among Iranian isolates. Further studies are needed to investigate the putative association of various tcdC genotypes with CDI severity and its recurrence.

scholarly journals Molecular Analysis of Aquaglyceroporin 1 Gene in Non-Healing Clinical Iso¬lates Obtained from Patients with Cutaneous Leishmaniasis from Central of Iran

2019 ◽  
Author(s):  
Yasaman Alijani ◽  
Saeedeh Sadat Hosseini ◽  
Salman Ahmadian ◽  
Sonia Boughattas ◽  
Gilda Eslami ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cutaneous Leishmaniasis ◽  
Molecular Characterization ◽  
Significance Level ◽  
Detection And Identification ◽  
Pcr Rflp ◽  
Leishmania Spp ◽  
Sensitive Isolate ◽  
The One

Background: Regarding the antimonial-resistant of Leishmania spp., understanding of related mechanism is neces­sary. One of the most important involved molecules is aquaglyceropin1 (AQP1). The aim of this study was molecu­lar analysis of AQP1 gene from antimonial-resistant clinical isolates and its expression. Methods: Overall, 150 patients with cutaneous leishmaniasis referring to the reference laboratories of Yazd and Varzaneh,, located 105km southeast of Isfahan and 240km away from Yazd, were assessed from Jun 2015 to Dec 2017. After sampling, staining was done and evaluated for Leishman by microscope. Samples were collected in RNAlater solution for gene expression analysis in non-healing isolates. DNA extraction was performed from each slide with Leishman body. All patients with L. major isolates detected by ITS1-PCR-RFLP were followed for find­ing the resistant isolates, consequence of molecular characterization of AQP1 using PCR-RFLP. Gene expression of AQP1 from all resistant isolates was assessed in comparison with the one in a sensitive isolate. Statistical analysis was done using SPSS. The significance level was considered ≤0.05. Results: Five isolates were detected as antimonial resistant. Molecular detection and identification were appeared that all were L. major. The molecular characterization of AQP1 showed G562A mutation. Gene expression of AQP1 in resistant isolates showed 1.67 fold higher than the sensitive isolate. Conclusion: We reported a new point mutation of G562A in AQP1 gene involved in molecular mechanism in re­sistant isolates.

Molecular characterization of Ovar-DRB1 exon2 gene in Garole sheep resilient to gastrointestinal nematodes

2015 ◽  
Author(s):  
C. Paswan ◽  
L. L.L. Prince ◽  
R. Kumar ◽  
C. P. Swarnkar ◽  
D. Singh ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Molecular Characterization ◽  
Molecular Mechanisms ◽  
Gastrointestinal Nematodes ◽  
Exon 2 ◽  
Pcr Rflp

Ovine MHC DRB1 exon 2 (Ovar-DRB1.2) gene is arguably one of the critical genes, responsible for disease resistance against parasite in animals. This study was carried out for indepth analysis of polymorphism in MHC DRB1.2 gene and to explore the underlying molecular mechanisms for the development of disease resistance in Garole. PCR-RFLP study revealed that the gene is polymorphic in nature. The frequency of allele ‘A’ for endonuclease SacI and Allele ‘B’ for endonuclease Hin1I were significantly higher in Garole population.

scholarly journals Molecular characterization of 'Candidatus Phytoplasma solani' in celery: Case study in Futog

2021 ◽  
Vol 58 (2) ◽  
pp. 66-71
Author(s):  
Tatjana Popović ◽  
Petar Mitrović ◽  
Andrea Kosovac
Keyword(s):  
Molecular Characterization ◽  
Gene Detection ◽  
Convolvulus Arvensis ◽  
Visual Evaluation ◽  
Leaf Yellowing ◽  
Pcr Rflp ◽  
Novi Sad ◽  
Stolbur Phytoplasma

'Candidates Phytoplasma solani', known by its trivial name stolbur phytoplasma, is a plant pathogen infecting numerous crops in Serbia. Celery plants with prominent leaf yellowing and chlorosis, sporadically with tissue necrosis, were observed during August 2020 in Futog, situated in Novi Sad suburbia in Vojvodina. Total of 12 sampled celery plants, 8 symptomatic and 4 asymptomatic ones, were analysed for 'Ca. P. solani' presence. All symptomatic celery plants were infected with stolbur phytoplasma according to the stoll 1 gene detection and therefore were further subjected to multigene molecular characterization on three genes: tuf, stamp and vmpl. Combining molecular tools PCR/RFLP and sequencing reviled two 'Ca. P. solani' multilocus genotypes in celery: tuf-b/Rqg31/V14 and tuf-b/ Rpm35/V14, present in 4 samples each. Obtained results of the strain genotyping are in concordance with previous data on the 'Ca. P. solani' diversity on celery, but supplemented with genotyping of the vmpl gene. Outbreak of stolbur phytoplasma in the assessed locality in Futog is linked to tuf-b epidemiological cycle correlated in Serbia mainly with weed Convolvulus arvensis which was present in the subjected celery plot, and could have been the phytoplasma inoculum source. Visual evaluation of the symptom occurrence suggests on 10-15% of 'Ca. P. solani' affected celery plants scattered throughout the plot corresponding to the pathogen dispersal in crop by cixiid planthoper Hyalesthes obsoletus (Hemiptera: Auchenorrhyncha) associated with C. arvensis, main vector of stolbur phytoplasma in Serbia.

Molecular characterization of INH-resistant Mycobacterium tuberculosis isolates by PCR-RFLP and multiplex-PCR in North India

2009 ◽  
Vol 9 (6) ◽  
pp. 1352-1355 ◽  
Author(s):  
Jitendra Prasad Mathuria ◽  
Gopal Nath ◽  
Jai Kumar Samaria ◽  
Shampa Anupurba
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Multiplex Pcr ◽  
Molecular Characterization ◽  
North India ◽  
Pcr Rflp

Occurrence and molecular characterization of Staphylococcus aureus strains isolated from meat and dairy products by PCR-RFLP

2010 ◽  
Vol 60 (2) ◽  
pp. 189-196 ◽  
Author(s):  
Mohammad Mehdi Soltan Dallal ◽  
Zohreh Salehipour ◽  
Saeed Eshraghi ◽  
Jalil Fallah Mehrabadi ◽  
Ronak Bakhtiari
Keyword(s):  
Staphylococcus Aureus ◽  
Molecular Characterization ◽  
Dairy Products ◽  
Pcr Rflp

Molecular characterization of potato virus YN isolates by PCR-RFLP

1996 ◽  
Vol 102 (7) ◽  
pp. 655-662 ◽  
Author(s):  
L. Glais ◽  
C. Kerlan ◽  
M. Tribodet ◽  
V. Marie -Jeanne Tordo ◽  
C. Robaglia ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Potato Virus ◽  
Pcr Rflp

A novel PCR-RFLP assay for molecular characterization of Echinococcus granulosus sensu lato and closely related species in developing countries

2016 ◽  
Vol 115 (10) ◽  
pp. 3817-3824 ◽  
Author(s):  
Raja Chaâbane-Banaoues ◽  
Myriam Oudni-M’rad ◽  
Selim M’rad ◽  
Hizem Amani ◽  
Habib Mezhoud ◽  
...  
Keyword(s):  
Developing Countries ◽  
Molecular Characterization ◽  
Echinococcus Granulosus ◽  
Related Species ◽  
Closely Related Species ◽  
Pcr Rflp ◽  
Rflp Assay ◽  
Echinococcus Granulosus Sensu Lato

Molecular Characterization of Aster Yellows Phytoplasma Associated with Valerian and Sowthistle Plants by PCR-RFLP Analyses

2008 ◽  
Vol 156 (6) ◽  
pp. 326-331 ◽  
Author(s):  
A.-H. Khadhair ◽  
C. Hiruki ◽  
M. Deyholos
Keyword(s):  
Molecular Characterization ◽  
Aster Yellows ◽  
Pcr Rflp ◽  
Aster Yellows Phytoplasma
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