scholarly journals Preliminary Phytochemical Screening and Bioactive Compounds of Swietenia macrophylla Seed Support T1DM and T2DM

2021 ◽  
Vol 7 (1) ◽  
pp. 267-271
Author(s):  
M. Chandrabalan Kamaraj ◽  
Ramakrishnan Akshaya ◽  
Dandapani Sudhakaran Iyer
Keyword(s):  
Antioxidant Activity ◽  
Molecular Docking ◽  
Free Radical ◽  
Radical Scavenging ◽  
Docking Studies ◽  
Free Radical Scavenging ◽  
Seed Extract ◽  
Phytochemical Screening ◽  
Swietenia Macrophylla ◽  
Molecular Docking Studies

The purpose of this study was to investigate the antidiabetic effect of phytocompounds from Swietenia macrophylla seed using preliminary phytochemical screening, invitro antioxidant activity and molecular docking studies. The powdered seed extract of Swietenia macrophylla was to investigate the phytochemical screening exhibited the presence of alkaloid, phenols, tannins, flavonoids, terpenoids, steroids, carbohydrates, amino acids and proteins as major active constituents. The antioxidant activity of Swietenia macrophylla seed was evaluated by DPPH free radical scavenging assay. Rutin was used as a reference compound. The Swietenia macrophylla seed exhibited 56.0471% of free radical scavenging activity as compared with rutin. The molecular docking studies performed by using molecular docking server online respectively in which the antidiabetic target namely glutamine:fructose-6-phosphate amidotransferase (GFAT) (PDB id: 2ZJ3) have a potential interaction with swietenine, swietenolide, β-sitosterol, and fucosterol. In this study, the protein glutamine:fructose-6-phosphate amidotransferase (GFAT) was used from its structure perspectives. Its primary and secondary structures were evaluated using online tools. Its role in antidiabetic was assessed by molecular docking the compounds present in the seed extract of Swietenia macrophylla assayed by GC-MS analysis. This in-silico study demonstrates the interactions of active components of Swietenia macrophylla against Type I and Type II diabetes.

scholarly journals Phytochemical screening and In-vitro antioxidant activity of Centella asiatica extracts

2017 ◽  
Vol 9 (4) ◽  
pp. 615
Author(s):  
Mukesh Kumar Yadav ◽  
Santosh Kumar Singh ◽  
JS Tripathi ◽  
YB Tripathi
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Centella Asiatica ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Phytochemical Screening

<p><em>Centella asiatica</em> also known as <em>mandukparni </em>or Indian pennywort or <em>jalbrahmi</em>, which has been used as a medicine in the Ayurveda from ancient times and mentioned in many classical texts of Ayurveda. <em>Centella asiatica</em> has long been used to improve memory and cognitive function.</p><p>The study aimed to identify the phytochemicals present in different solvent extracts of <em>Centella asiatica </em>(i.e. PECA- Petroleum ether extract of <em>C. asiatica, </em>CCA- Chloroform extract of <em>C. asiatica, </em>EACA- Ethyl acetate extract of <em>C. asiatica,</em> ECA- Ethanolic extract of <em>C. asiatica, </em>HACA- Hydro-alcoholic extract of <em>C. asiatica</em>)<em> </em>and evaluate the respective in-vitro antioxidant potentials. <em></em></p><p>The phytochemical screening of extracts was done with standardized procedures and the antioxidant potential of different solvent extracts of <em>Centella asiatica</em> was assessed by its free radical scavenging activity 2, 2-diphenyl -1- picrylhydrazyl (DPPH) as well as hydrogen peroxide scavenging assay respectively for reducing capability.</p><p>In all different solvent extracts of <em>C. asiatica</em> revealed excellent free radical scavenging activity as revealed by 2-2- diphenyl-1-picryl-hydrazyl (DPPH) assay with  EC<sub>50</sub> values for ECA=128.752±1.85 μg/ml, HACA=274.884±1.21 μg/ml and hydrogen peroxide assay against the standard (Butylated hydroxytoluene) BHT, with the EC<sub>50</sub> values ECA=429.69±0.92 μg/ml HACA=458.08±0.58 μg/ml while rest solvent extracts shown very less antioxidant activity.</p><p> The present study indicates that the <em>Centella asiatica</em> extracts have good antioxidant activity which can be used in stress and anxiety and also a good source to be used as natural drugs.</p>

scholarly journals Phytochemical screening, free radical scavenging, antioxidant activity and phenolic content of Dodonaea viscosa

2012 ◽  
Vol 77 (4) ◽  
pp. 423-435 ◽  
Author(s):  
Tauheeda Riaz ◽  
Athar Abbasi ◽  
A Aziz-Ur-Rehman ◽  
Tayyaba Shahzadi ◽  
Muhammad Ajaib ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Antioxidant Activity ◽  
Phytochemical Screening ◽  
Dpph Radical ◽  
Dodonaea Viscosa ◽  
Total Antioxidant

The purpose of this study was to evaluate the antioxidant potential of Dodonaea viscosa Jacq. Methanolic extract of the plant was dissolved in distilled water and partitioned with n-hexane, chloroform, ethyl acetate and nbutanol sequentially. Phytochemical screening showed presence of phenolics, flavonoides and cardiac glycosides in large amount in chloroform, ethyl acetate and n-butanol fraction. The antioxidant potential of all these fractions and remaining aqueous fraction was evaluated by four methods: 1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, total antioxidant activity, Ferric Reducing Antioxidant Power (FRAP) assay and ferric thiocyanate assay along with determination of their total phenolics. The results revealed that ethyl acetate soluble fraction exhibited highest percent inhibition of DPPH radical as compared to other fractions. It showed 81.14 ? 1.38% inhibition of DPPH radical at a concentration of 60 ?g/ml. The IC50 of this fraction was found to be 33.95 ? 0.58 ?g/ml, relative to butylated hydroxytoluene (BHT), having IC50 of 12.54 ? 0.89 ?g/mL. It also showed highest FRAP value (380.53 ? 0.74 ?M of trolox equivalents) as well as highest total phenolic contents (208.58 ? 1.83 GAE ?g/g) and highest value of inhibition of lipid peroxidation (58.11 ? 1.49% at concentration of 500 ?g/ml) as compared to the other studied fractions. The chloroform fraction showed highest total antioxidant activity i.e.1.078 ? 0.59 (eq. to BHT).

scholarly journals Nutraceutical studies on Eriobotrya japonica (Thunb.) Lindl. (Fruits & Seeds)

2017 ◽  
Vol 3 (4) ◽  
pp. 44
Author(s):  
Rajalakshmi P ◽  
Pugalenthi M ◽  
Subashini G ◽  
Miss. Kavitha ◽  
Vishnukumar S
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Eriobotrya Japonica ◽  
Phytochemical Screening ◽  
Total Carbohydrate ◽  
Nutritional Parameters ◽  
Wide Range

Fruits and vegetables have plenty of natural antioxidants, especially vitamin C and E. Fruits contained beta-carotene, phenolic compounds such as anthocyanin and other flavonoids, which showcase a wide range of biological benefits, including antioxidant, anti-inflammatory and anti carcinogenic properties. Eriobotrya japonica commonly called as loquat fruit, is a subtropical evergreen fruit. In the present study an attempt was made to analyzed the phytochemical screening, nutraceuticals and antioxidant activity of the aforesaid traditionally important and pharmacologically potent fruits and seeds of E. japonica. The dried powdered fruits and seed samples were subjected for the analysis of nutritional parameters, phytochemical screening and evaluation of antioxidant activity. The nutritional parameters such as total carbohydrate, total starch, total proteins and levels of free amino acids were analyzed. Phytochemical screening of E. japonica clearly showed the presence of certain important secondary metabolites such as alkaloids, phenols, flavonoids, glycosides, cardiac glycosides, phytosterol and gums and mucilage. The antioxidant activity of the plant extracts were determined by using super oxide free radical scavenging assay. The results of the present study clearly indicated that methanol seed extract showed better free radical scavenging activity at concentration of 1000 mg/ml of extract. Among the selected samples fruits of E.japonica was found to be more potent than the seeds of the same species. However, more advanced pharmacological and clinical studies would be required to investigate in vivo mechanism of pharmacological effects of this important edible fruits E. japonica.

scholarly journals Phytochemical analysis, mineral composition and in vitro antioxidant activities of Solanum macrocarpon leaves

2016 ◽  
Vol 4 (1) ◽  
pp. 62
Author(s):  
Usunomena Usunobun ◽  
Igwe V. Chinwe
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Phytochemical Screening ◽  
Mineral Analysis

Background: The aim of this study is to determine phytochemicals and mineral composition as well as in vitro antioxidant activities of Solanum macrocarpon leaves.Methods: Qualitative phytochemical screening was carried out using standard procedures while Mineral analysis was carried out using Atomic Absorption Spectrophotometer (AAS). Solanum macrocarpon leaves were also subjected for measurement of reducing power and antioxidant/radical scavenging activity (2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity).Results: Phytochemical screening revealed the presence of flavonoids, saponins, alkaloids etc. Mineral analysis showed calcium (256.60mg/100g) to be higher in concentration and copper (0.62mg/100g) least in concentration while manganese was absent. Other minerals includes magnesium (81.69mg/100g), potassium (87.22mg/100g), sodium (32.51mg/100g), iron (31.41mg/100g), zinc (1.41mg/100g). Solanum macrocarpon leaves showed maximum antioxidant activity (DPPH free radical scavenging and reducing power capacity) as the higher the concentration, the higher the antioxidant activity, thus the better the free radical scavenging potentials.Conclusion: The data from this study revealed that Solanum macrocarpon has a rich content of phytochemicals, namely, saponins, alkaloids, flavonoids as well as minerals, bioactive components that are associated with health impacts. This study also revealed that Solanum macrocarpon leaves exhibit antioxidant activity. These findings thus suggest that Solanum macrocarpon leaves could act as potent source of antioxidants.

Free Radical Scavenging and Antioxidant Activity of Silver Nanoparticles Synthesized from Cuminum cyminum (Cumin) seed Extract

2021 ◽  
pp. 4349-4354
Author(s):  
Lubna Abdulazeem ◽  
Mohammad J. AL Jassani ◽  
Mustafa A. Al-Sheakh
Keyword(s):  
Antioxidant Activity ◽  
Silver Nanoparticles ◽  
Free Radical ◽  
Large Scale ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Seed Extract ◽  
Sem Images ◽  
Cumin Seed

Silver nanoparticles are extensive, applied in different fields. Green methods using plants have been used as renewable resources for the synthesis of biodegraded nanomaterials, thereby providing economic and safe synthesis routes. The green synthesis was done using the aqueous Cumin seed extract and as a bio-reducer agent and aqueous AgNO3 solution as a precursor under various conditions. The formation of silver nanoparticles was confirmed by the observation of the shift in color from colorless to dark brown. The synthesized AgNPs were characterized using UV/V is spectroscopy, XRD, FTIR, and SEM. Also, the synthesized AgNPs were evaluated for their antioxidant activity (In-vitro) by DPPH assay. The bio-reduced mixture showed a maximum peak at around 388nm. The XRD peaks were observed at 38o and 46o, corresponding to 111, 200, 220, and 311, and the peak widening suggested a smaller particle size. The FTIR absorption spectra indicated the presence of residual plant extract as a reducing agent in the reaction mixture. Also, analysis of C. cyminum seed extract strongly suggested the presence of OH stretching in alcoholic and phenolic compounds as the main phytochemicals parts, which is supported by a strong peak at approximately 3296cm−1. The SEM images clearly showed that AgNPs were almost spherical in shape and 48.7nm in size. The synthesized AgNPs showed almost the same pattern of ascorbic acid-free radical scavenging activity except at concentrations 100 and 50µg/ml with significant differences (P≤0.05) and it is dose-dependent. Silver nanoparticles can be synthesized on a large scale following a simple and eco-friendly method using C. cyminum seed extract that can be used as an effective antioxidant.

scholarly journals Synthesis, Characterization, Biological Evaluation and Molecular Docking Studies of Some Oxazinyl-Thiazolidinone Derivatives

2020 ◽  
Vol 32 (9) ◽  
pp. 2125-2129
Author(s):  
RAMARAJAN RAJALAKSHMI ◽  
RAJAVEL SANTHI ◽  
THANGARAJ ELAKKIYA
Keyword(s):  
Antioxidant Activity ◽  
Molecular Docking ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Docking Studies ◽  
Biological Evaluation ◽  
Molecular Docking Studies ◽  
Ir Studies ◽  
Wide Range

A series of new 4-thiazolidinone derivatives of 2-(4-chlorophenyl)-3-(6-(thiophen-2-yl)-4-p-tolyl-4H-1,3-oxazin-2-yl)- thiazolidin-4-one (7h-m) are synthesized because of its wide range of biological activities.1H & 13C NMR, IR studies were applied for the elucidation of all the synthesized compounds. All the synthesized compounds have been tested for antidiabetic and antioxidant activity in vitro method against standard. The analogs 7h-m was evaluated for α-amylase and α-glucosidase inhibitory potential. The structures of all the compounds have been screened for antioxidant activity using DPPH radical scavenging assay, NO scavenging method. Molecular docking studies were accomplished in addition to understand the binding affinity of those compounds with PDBID 2HR7 which showed that the synthesized derivatives bind in the lively binding site of the target protein

Changes in antioxidant activity and free radical scavenging potential of rosemary extract and tocopherols in isolated rapeseed oil triacylglycerols during accelerated tests

2005 ◽  
Vol 93 (2) ◽  
pp. 227-235 ◽  
Author(s):  
Malgorzata Nogala-Kalucka ◽  
Jozef Korczak ◽  
Małgorzata Dratwia ◽  
Eleonora Lampart-Szczapa ◽  
Aleksander Siger ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Rapeseed Oil ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Rosemary Extract ◽  
Accelerated Tests

Antioxidant Activity of Silk Fibroin Peptide Hydrolyzed by Pancreatin

2014 ◽  
Vol 1081 ◽  
pp. 110-114 ◽  
Author(s):  
Zhen Zhu ◽  
Hua Yin ◽  
Yan An
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Nitrogen Content ◽  
Silk Fibroin ◽  
Radical Scavenging ◽  
Amino Nitrogen ◽  
Free Radical Scavenging ◽  
Hydrolysis Process ◽  
Superoxide Free Radical ◽  
Hydrolysis Of

This research adopts the pancreatin hydrolysis of silk fibroin active peptide, evaluate the antioxidant activity of hydrolysates. In the process of hydrolysis of silk fibroin, by measuring the amino nitrogen content of neutral formaldehyde titration method. Find the amino nitrogen content gradually stabilized at around 0.37g/L, and superoxide free radical scavenging rate changing with time fluctuation trend, superoxide free radical scavenging rate to a maximum of 65.03% at 220min.The use of silk fibroin hydrolysis process optimization,reaction time 160min, enzyme concentration4% , substrate concentration 20mg/ml, pH 8, temperature 38°C. The hydrolysis process under the hydrolysate on superoxide radical scavenging rate of 72.73%. The scavenging rate of hydroxyl radical is 47.24%. Red blood cell hemolysis induced by H2O2 inhibition rate was 24.30%.

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