scholarly journals Phytochemical analysis, mineral composition and in vitro antioxidant activities of Solanum macrocarpon leaves

2016 ◽  
Vol 4 (1) ◽  
pp. 62
Author(s):  
Usunomena Usunobun ◽  
Igwe V. Chinwe
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Phytochemical Screening ◽  
Mineral Analysis

Background: The aim of this study is to determine phytochemicals and mineral composition as well as in vitro antioxidant activities of Solanum macrocarpon leaves.Methods: Qualitative phytochemical screening was carried out using standard procedures while Mineral analysis was carried out using Atomic Absorption Spectrophotometer (AAS). Solanum macrocarpon leaves were also subjected for measurement of reducing power and antioxidant/radical scavenging activity (2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity).Results: Phytochemical screening revealed the presence of flavonoids, saponins, alkaloids etc. Mineral analysis showed calcium (256.60mg/100g) to be higher in concentration and copper (0.62mg/100g) least in concentration while manganese was absent. Other minerals includes magnesium (81.69mg/100g), potassium (87.22mg/100g), sodium (32.51mg/100g), iron (31.41mg/100g), zinc (1.41mg/100g). Solanum macrocarpon leaves showed maximum antioxidant activity (DPPH free radical scavenging and reducing power capacity) as the higher the concentration, the higher the antioxidant activity, thus the better the free radical scavenging potentials.Conclusion: The data from this study revealed that Solanum macrocarpon has a rich content of phytochemicals, namely, saponins, alkaloids, flavonoids as well as minerals, bioactive components that are associated with health impacts. This study also revealed that Solanum macrocarpon leaves exhibit antioxidant activity. These findings thus suggest that Solanum macrocarpon leaves could act as potent source of antioxidants.

scholarly journals Free radical scavenging ability of a potent therapeutic plant mussaenda luteola delile (rubiaceae)

2021 ◽  
Vol 8 (1) ◽  
pp. 69-72
Author(s):  
Betty T ◽  
Sumathi P ◽  
Indhumathi T ◽  
Prabavathi B ◽  
Devadharshini B
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Leaf Extracts

The aim of present study was to investigate the in vitro antioxidant potential and total extractive yield of Mussaenda luteola Delile leaves. Antioxidant activity was assessed by using 2,2- diphenyl-1-picryl-hydrazyl (DPPH• ) assay, reducing power activity and [2,2’-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid)] ABTS•+ assay. Here ascorbic acid (ASA) and rutin were used as standard antioxidants. The results of the study indicates that the chloroform extracts of the leaf of Mussaenda luteola possesses significant scavenging activity against DPPH• (17.56) and reducing power activity (0.759) at 700nm absorbance. The ethanolic leaf extracts holds high free radical scavenging activity (ABTS•+) at 735nm (94.59). The free radical scavenging and antioxidant activities may be attributed to the presence of adequate phenolic and flavonoid compounds. The ethanolic leaf extract of M. luteola yields maximum extractive yield percentage (37.08%). This study revealed that the leaf extracts of Mussaenda luteola has demonstrated significant antioxidant activity.

scholarly journals Phytochemical screening and In-vitro antioxidant activity of Centella asiatica extracts

2017 ◽  
Vol 9 (4) ◽  
pp. 615
Author(s):  
Mukesh Kumar Yadav ◽  
Santosh Kumar Singh ◽  
JS Tripathi ◽  
YB Tripathi
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Centella Asiatica ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Phytochemical Screening

<p><em>Centella asiatica</em> also known as <em>mandukparni </em>or Indian pennywort or <em>jalbrahmi</em>, which has been used as a medicine in the Ayurveda from ancient times and mentioned in many classical texts of Ayurveda. <em>Centella asiatica</em> has long been used to improve memory and cognitive function.</p><p>The study aimed to identify the phytochemicals present in different solvent extracts of <em>Centella asiatica </em>(i.e. PECA- Petroleum ether extract of <em>C. asiatica, </em>CCA- Chloroform extract of <em>C. asiatica, </em>EACA- Ethyl acetate extract of <em>C. asiatica,</em> ECA- Ethanolic extract of <em>C. asiatica, </em>HACA- Hydro-alcoholic extract of <em>C. asiatica</em>)<em> </em>and evaluate the respective in-vitro antioxidant potentials. <em></em></p><p>The phytochemical screening of extracts was done with standardized procedures and the antioxidant potential of different solvent extracts of <em>Centella asiatica</em> was assessed by its free radical scavenging activity 2, 2-diphenyl -1- picrylhydrazyl (DPPH) as well as hydrogen peroxide scavenging assay respectively for reducing capability.</p><p>In all different solvent extracts of <em>C. asiatica</em> revealed excellent free radical scavenging activity as revealed by 2-2- diphenyl-1-picryl-hydrazyl (DPPH) assay with  EC<sub>50</sub> values for ECA=128.752±1.85 μg/ml, HACA=274.884±1.21 μg/ml and hydrogen peroxide assay against the standard (Butylated hydroxytoluene) BHT, with the EC<sub>50</sub> values ECA=429.69±0.92 μg/ml HACA=458.08±0.58 μg/ml while rest solvent extracts shown very less antioxidant activity.</p><p> The present study indicates that the <em>Centella asiatica</em> extracts have good antioxidant activity which can be used in stress and anxiety and also a good source to be used as natural drugs.</p>

In Vitro Preliminary Phytochemical Screening and Free Radical Scavenging Ability of Drosera indica L.

2017 ◽  
Vol 9 (5) ◽  
Author(s):  
K. R. Asha ◽  
S. Hemmalakshmi ◽  
S. Priyanga ◽  
K. Devaki
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Phytochemical Screening ◽  
Dpph Radical ◽  
Whole Plant

Aim: The present study is carried out to explore the preliminary phytochemical screening and free radical scavenging activity of the whole plant Drosera indica L. Methods: a) Phytochemical screening – The qualitative analysis of secondary metabolites is carried out by the standard qualitative methods. b) In vitro free radical scavenging activity of the ethanolic and aqueous extract of the whole plant Drosera indica L is used for the analysis .Various concentrations (100 – 500mcg/ml) of the ethanol and aqueous extracts of Drosera indica L. are used in the various antioxidant assay methods such as reducing power, ferric reducing antioxidant power assay (FRAP), nitric oxide (NO) radical,2,2’ azinobis-3 ethylbenzothiozoline-6 sulfonic acid (ABTS+) radical, hydroxyl radical (OH.), 1,1-diphenyl-2-picryl hydroxyl (DPPH) radical , super oxide radical and hydrogen peroxide (H2O2) is carried out with the standard protocols. In all the assays ascorbic acid is used as the standard antioxidant. Results: Phytochemical screening of the plants reveal the presence of numerous chemicals including flavanoids, tannins, polyphenols, cardiac glycosides and saponins. The ethanolic extract of Drosera indica L. shows better ability to scavenge ,1,1-diphenyl-2-picryl hydroxyl( DPPH)radical, hydroxyl radical, hydrogen peroxide, nitric oxide radical and superoxide radical. FRAP and the reducing power abilities of the ethanolic extract is increased with the increase in concentration of the plant extract. Conclusion: The ethanolic extract of Drosera indica L. shows better ability to scavenge the free radicals than the aqueous extract. From this study, a conclusion is drawn that Drosera indica L. can have more beneficial effects with respect to the presence of many active secondary metabolites which may likely to combat with the oxidative stress diseases like diabetes, cancer, cardio-vascular diseases and in general boost the immune system.

scholarly journals Phytochemical screening, mineral composition and in vitro antioxidant activities of Pterocarpus mildbraedii leaves

2016 ◽  
Vol 4 (1) ◽  
pp. 23 ◽  
Author(s):  
Usunomena Usunobun ◽  
Igwe V. Chinwe
Keyword(s):  
Therapeutic Potential ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Property ◽  
Phytochemical Screening ◽  
Mineral Analysis ◽  
Biochemical Basis

Background: Pterocarpus mildbraedii is used in some Nigerian tribes in the treatment of headaches, pains, fever, convulsions, and respiratory disorders. This study was carried out to ascertain the phytochemicals, minerals and in vitro antioxidant activities of Pterocarpus mildbraedii leaves.Methods: Qualitative phytochemical screening was carried out using standard procedures while Mineral analysis was carried out using Atomic Absorption Spectrophotometer (AAS). Also, antioxidant activities of Pterocarpus mildbraedii leaves were evaluated using reducing power ability and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays.Results: Result on phytochemicals reveals the presence of flavonoids, saponins, alkaloids, tannins. Mineral analysis showed calcium (1283mg/kg) to be higher in concentration and manganese (4mg/kg) least in concentration (4mg/kg). Other analysed minerals include sodium (603mg/kg), potassium (1048mg/kg), magnesium (778mg/kg), zinc (25mg/kg), iron (146mg/kg), phosphates (1002mg/kg), etc. In vitro antioxidant activity results show that Pterocarpus mildbraedii possess potent but dose-dependent reducing power ability and free radical scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals.Conclusion: The presence of phytochemicals such as flavonoids, saponins, alkaloids and tannins in Pterocarpus mildbraedii provides some biochemical basis for the ethno pharmacological uses of the plant. Also the information provided in this research study may help to spread the ethno botanical uses of Pterocarpus mildbraedii leaves to other parts of the World. The findings clearly demonstrate that Pterocarpus mildbraedii leaves have promising antioxidant property and needs further evaluation to exploit its therapeutic potential.

scholarly journals EVALUATION OF ANTIOXIDANT AND FREE RADICAL SCAVENGING ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS OF LEAVES OF PIPER UMBELLATUM

2017 ◽  
Vol 10 (2) ◽  
pp. 274
Author(s):  
Sethupandian Geetha ◽  
Kokkaiah Irulandi ◽  
Palanichamy Mehalingam
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Frap Assay ◽  
Radical Scavenging Properties

Objective: This study was designed to determine the Total phenol, flavonoid content, antioxidant and free radical scavenging properties of different solvent extracts of Piper umbellatum.  Methods: Different solvent extracts evaluated with DPPH radical scavenging activity and Reducing power activity.Results: The presence of phenol and flavonoid showed highly in the methanol extracts than ethyl acetate and acetone extracts. All the extracts have various level of antioxidant activity. Methanol solvent extract have good extraction and show significant antioxidant activity. The effect of reducing power of methanol extract revealed good antioxidant activity compare with other tested extracts.Conclusion: On the basis of the above results we concluded that methanol extract of Piper umbellatum whole plant extracts shows significant antioxidant activity than ethyl acetate and acetone extracts.Keywords: Piper umbellatum, Antioxidant, DPPH, FRAP assay, Polar, Non polar solvents

Evaluation of antioxidant activity of Melothria maderaspatana in vitro

2010 ◽  
Vol 5 (2) ◽  
pp. 224-230 ◽  
Author(s):  
Boobalan Raja ◽  
Kodukkur Pugalendi
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Free Radical ◽  
Hydroxyl Radical ◽  
Superoxide Anion ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Melothria Maderaspatana

AbstractIn this study, an aqueous extract of leaves from Melothria maderaspatana was tested for in vitro antioxidant activity. Free radical scavenging assays, such as hydroxyl radical, hydrogen peroxide, superoxide anion radical and 2,2-diphenyl-1-picryl hydrazyl (DPPH), 2,2’-azinobis-(3-ethyl-enzothiazoline-6-sulfonic acid) (ABTS) radical scavenging, and reducing power assay, were studied. The extract effectively scavenged hydroxyl radical, hydrogen peroxide and superoxide anion radicals. It also scavenged DPPH and ABTS radicals. Furthermore, it was found to have reducing power. All concentrations of leaf extract exhibited free radical scavenging and antioxidant power, and the preventive effects were in a dose-dependent manner. The antioxidant activities of the above were compared to standard antioxidants such as butylated hydroxytoluene (BHT), ascorbic acid, and α-tocopherol. The results obtained in the present study indicate that the M. maderaspatana extract could be considered a potential source of natural antioxidant.

scholarly journals Evaluation of Free Radical Scavenging and Polyphenolic Contents of Bark of Pterygota alata Roxb

2014 ◽  
Vol 6 (3) ◽  
pp. 543-552 ◽  
Author(s):  
N. Jahan ◽  
M. S. Parvin ◽  
A. Khan ◽  
N. Das ◽  
M. S. Islam ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Soluble Fraction ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Deciduous Tree ◽  
Total Phenolic ◽  
Phenolic And Flavonoid

Pterygota alata is a large deciduous tree of Malvaceae family. The present study was designed to evaluate in vitro antioxidant activity of stem bark of the plant. Ferric reducing power (FRP) test, 1, 1- diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging test and total antioxidant capacity (TAC) assay were used to detect the antioxidant activity. Total phenolic and flavonoid contents were also determined as they are well known phytochemicals with antioxidant property. The bark of the plant showed efficient reducing power as well as free radical scavenging property (IC50 values 52.25 - 172.05). The bark also found rich in total phenolic and flavonoid content. The highest amount of total phenolic content was found in chloroform soluble fraction (29.898 µg/mL) followed by ethyl acetate soluble fraction (15.88 µg/mL). The highest content of total flavonoid also detected in chloroform soluble fraction (107.56 µg/mL) but followed by crude ethanol extract (98.66 µg/mL). Overall, the bark of the plant possesses significant antioxidant activity, therefore can be used as a good natural source of antioxidant. © 2014 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. doi: http://dx.doi.org/10.3329/jsr.v6i3.17586 J. Sci. Res. 6 (3), 543-552 (2014)

scholarly journals Preliminary Phytochemical Screening and In vitro Antioxidant Activity of Ethiopian Indigenous Medicinal Plants, Ocimum lamiifolium Hochst. ex Benth and Ocimum basilicum L.

2016 ◽  
Vol 8 (01) ◽  
Author(s):  
Suresh Kumar P. Nair ◽  
Kumar Ganesan ◽  
Henok Azalewor ◽  
Neethu Letha ◽  
Sharmila Gani
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Free Radical Scavenging ◽  
Ocimum Basilicum ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
Phytochemical Investigation

Antioxidants are the chemical substances which prevent the free radicals damage in the body. Numerous researches are going globally focussed on investigating natural antioxidants of plants origins. The aims of the present study were to evaluate preliminary phytochemical investigation and in vitro antioxidant activities of Ethiopian indigenous medicinal plants, Ocimum lamiifolium Hochst. ex Benth and Ocimum basilicum L. Aqueous, benzene and hexane crude leaves extracts of O. lamiifolium and O. basilicum were subjected to qualitative phytochemical screening using standard procedures. In addition, we investigated the antioxidant potential of crude aqueous leaves extract of O. lamiifolium and O. basilicum using tests involving inhibition of superoxide anions, DPPH, H2O2, NO and ABTS. Preliminary phytochemical investigation for benzene, hexane and aqueous extracts found alkaloids, sterols, carbohydrate and glycosides, tannins and flavonoids. The fraction inhibition of lipid peroxide at the first stage of oxidation illustrated antioxidant activity of O. lamiifolium and O. basilicum as 90% and 88% compared to those of gallic acid (97%) and BHT (84%) respectively. Also, the aqueous leaves extract of O. lamiifolium and O. basilicum exhibited significant DPPH free radical scavenging activity, nitric acid free radical scavenging activity assay, superoxide anion scavenging activity, ABTS scavenging activity and hydrogen peroxide free radical scavenging assay. Our findings provide confirmation that the aqueous leaves extract of O. lamiifolium and O. basilicum are potential source of natural antioxidants, and this warranted its uses in traditional medicine systems.

scholarly journals Nutraceutical studies on Eriobotrya japonica (Thunb.) Lindl. (Fruits & Seeds)

2017 ◽  
Vol 3 (4) ◽  
pp. 44
Author(s):  
Rajalakshmi P ◽  
Pugalenthi M ◽  
Subashini G ◽  
Miss. Kavitha ◽  
Vishnukumar S
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Eriobotrya Japonica ◽  
Phytochemical Screening ◽  
Total Carbohydrate ◽  
Nutritional Parameters ◽  
Wide Range

Fruits and vegetables have plenty of natural antioxidants, especially vitamin C and E. Fruits contained beta-carotene, phenolic compounds such as anthocyanin and other flavonoids, which showcase a wide range of biological benefits, including antioxidant, anti-inflammatory and anti carcinogenic properties. Eriobotrya japonica commonly called as loquat fruit, is a subtropical evergreen fruit. In the present study an attempt was made to analyzed the phytochemical screening, nutraceuticals and antioxidant activity of the aforesaid traditionally important and pharmacologically potent fruits and seeds of E. japonica. The dried powdered fruits and seed samples were subjected for the analysis of nutritional parameters, phytochemical screening and evaluation of antioxidant activity. The nutritional parameters such as total carbohydrate, total starch, total proteins and levels of free amino acids were analyzed. Phytochemical screening of E. japonica clearly showed the presence of certain important secondary metabolites such as alkaloids, phenols, flavonoids, glycosides, cardiac glycosides, phytosterol and gums and mucilage. The antioxidant activity of the plant extracts were determined by using super oxide free radical scavenging assay. The results of the present study clearly indicated that methanol seed extract showed better free radical scavenging activity at concentration of 1000 mg/ml of extract. Among the selected samples fruits of E.japonica was found to be more potent than the seeds of the same species. However, more advanced pharmacological and clinical studies would be required to investigate in vivo mechanism of pharmacological effects of this important edible fruits E. japonica.

Synthesis, Characterization, Free-radical Scavenging Capacity and Antioxidant Activity of Novel Series of Hydrazone, 1,3,4-oxadiazole and 1,2,4- triazole Derived from 3,5-dimethyl-1H-pyrazole

2019 ◽  
Vol 16 (7) ◽  
pp. 712-720 ◽  
Author(s):  
Khalid Karrouchi ◽  
Saad Fettach ◽  
Smaail Radi ◽  
El bekkaye Yousfi ◽  
Jamal Taoufik ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Anti Cancer ◽  
Ic50 Values

Background: Pyrazole is an important class of heterocyclic compound, has been shown to exhibit diverse biological and pharmacological activities such as anti-inflammatory, anti-cancer, antioxidant, etc. Methods: In this study, a series of novel 3,5-dimethyl-1H-pyrazole derivatives containing hydrazine 4a-l have been synthesized via the reaction of the 2-(3,5-dimethyl-1H-pyrazol-1-yl)acetohydrazide. All synthesized compounds have been tested for their in vitro antioxidant activities via utilization of 1,1-biphenyl-2-picrylhydrazyl (DPPH) as a free radical scavenging reagent. Results: The data reported herein indicates that compound 4k showed potential radical scavenging capacity and compounds 4f and 4g exhibited best activity for the iron binding while comparing with positive controls. Conclusion: Good activity was noted for some compounds. In particular, compound 4k showed the highest antioxidant activity with IC50 values of 22.79 ± 3.64 and 1.35 ± 0.66 μg/mL in the DPPH and ABTS tests, respectively.

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