scholarly journals Bacillus thuringiensis for integrated pest management in processing tomatoes

1995 ◽  
Vol 48 ◽  
pp. 289-293 ◽  
Author(s):  
P.J. Cameron ◽  
G.P. Walker ◽  
T.J.B. Herman ◽  
J.D. Fletcher
Keyword(s):  
Bacillus Thuringiensis ◽  
Integrated Pest Management ◽  
Pest Management ◽  
Processing Tomatoes

scholarly journals Synergy of Lepidopteran Nucleopolyhedroviruses AcMNPV and SpliNPV with Insecticides

Insects ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 316 ◽  
Author(s):  
Beatriz Dáder ◽  
Eduardo Aguirre ◽  
Primitivo Caballero ◽  
Pilar Medina
Keyword(s):  
Bacillus Thuringiensis ◽  
Integrated Pest Management ◽  
Pest Management ◽  
Spodoptera Littoralis ◽  
Spodoptera Exigua ◽  
Autographa Californica ◽  
Antagonistic Effects ◽  
Lepidopteran Pests ◽  
Excellent Control ◽  
Autographa Californica Multiple Nucleopolyhedrovirus

The joint use of baculoviruses and synthetic insecticides for integrated pest management requires the study of the additive, synergistic or antagonistic effects among them on pest mortality. Droplet bioassays were conducted with Autographa californica multiple nucleopolyhedrovirus (AcMNPV), Spodoptera littoralis nucleopolyhedrovirus (SpliNPV) and seven insecticides (azadirachtin, Bacillus thuringiensis, cyantraniliprole, emamectin, metaflumizone, methoxyfenozide and spinetoram) on Spodoptera exigua and Spodoptera littoralis. The lethal concentrations LC50 and LC95 were calculated through probit regressions. Then, the sequential feeding of insecticides and nucleopolyhedroviruses was studied. Larvae were provided with the LC50 of one insecticide, followed by the LC50 of one nucleopolyhedrovirus 24 h later. The inverse order was also conducted. The insecticide LC50 and LC95 were higher for S. littoralis than for S. exigua. AcMNPV showed greater toxicity on S. exigua than SpliNPV on S. littoralis. Emamectin showed synergy with AcMNPV when the chemical was applied first, and metaflumizone and AcMNPV were synergistic regardless of the order of application, both from the first day of evaluation. SpliNPV was synergistic with azadirachtin and emamectin when it was applied first, but synergy was reached after 12–13 days. Excellent control is possible with the LC50 of azadirachtin, emamectin and metaflumizone in combination with nucleopolyhedroviruses, and merits further study as a means of controlling lepidopteran pests.

scholarly journals Can Bacillus thuringiensis affect the biological variables of natural enemies of Lepidoptera?

2018 ◽  
Vol 85 (0) ◽  
Author(s):  
Gabriella Aparecida Salis de Carvalho ◽  
Daniel Júnior Martins ◽  
Isadora Mileny Costa de Brito ◽  
Sebastião Lourenço de Assis Júnior ◽  
Marcus Alvarenga Soares ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Integrated Pest Management ◽  
Pest Management ◽  
Pest Control ◽  
Wheat Bran ◽  
Natural Enemies ◽  
Oviposition Period ◽  
Control Methods ◽  
Biological Variables ◽  
Coleoptera Tenebrionidae

ABSTRACT: The entomopathogen Bacillus thuringiensis (Bt) is widely used as one of the ingredients in pest control formulations, but researches conducted on its effect on non-target organisms are still in the nascent stage. This investigation aimed to uncover if Bt treated with Tenebrio molitor (Coleoptera: Tenebrionidae) larvae and pupae could affect the biological variables of Podisus nigrispinus (Hemiptera: Pentatomidae) and Palmistichus elaeisis (Hymenoptera: Eulophidae), all of which established natural enemies of leaf defoliator caterpillars in the eucalyptus culture. Larvae of T. molitor were fed on wheat bran containing different concentrations of B. thuringiensis (0.00; 0.25; 0.50; 1.00; 2.00 and 4.00 g Agree/kg bran). When the larvae attained size of about 2 cm, they were used as prey for P. nigrispinus (Bioassay I), and their pupae used as hosts for P. elaeisis (Bioassay II). Only the biological variables oviposition period and egg numbers by posture of the predator P. nigrispinus were altered. The biological variables of P. elaeisis were not altered, since it was possible to use these control methods within the integrated pest management.

scholarly journals Formulasi bioinsektisida Bacillus thuringiensis isolat indigenos untuk pengendalian Hyposidra talaca pada tanaman teh (Formulation of indigenous isolate of Bacillus thuringiensis bioinsecticide to control Hyposidra talaca on tea)

2019 ◽  
Vol 87 (1) ◽  
Author(s):  
Happy WIDIASTUTI ◽  
TRI - PANJI ◽  
Ciptadi Achmad YUSUP ◽  
Iman RUSMANA ◽  
Tri Eko WAHYONO
Keyword(s):  
Bacillus Thuringiensis ◽  
Integrated Pest Management ◽  
Pest Management ◽  
Phase Contrast ◽  
Phase Contrast Microscope ◽  
Contrast Microscope ◽  
Preliminary Study ◽  
Hyposidra Talaca ◽  
The One ◽  
White Clay

A study has been conducted to develop indigenousBacillusthuringiensisbioinsecticide. Preliminary study has been conducted to explore B. thuringiensisfrom sample of soil, leaf, and infected larvae from selected tea area as well as another area such as cocoa, and acasia. The result showed that based on the colony morphology, it was found 10 isolateswith the characteristics of B. thuringiensis colony.  Assessed of the ability to formed crystal using phase contrast microscope and staining revealed that 4 isolates as crystal protein forming isolates. The four isolates used as active coumpound of bioinsecticide. The best formula based on the viability of bacteria was the one withwhite clay as a carrier. While the best liquid formula based on the similar characteristic was using maltosa as osmoprotectant. Theassessment ofthetoxisity B. thuringiensistowards Hyposidra talacalarvae showed that B. thuringiensiscould control H. talacalarvaeup to 37.5%. However, the toxicity need longer periode compared to that commercial B. thuringiensisbioinsecticide. [Keywords:Bt insecticide, cypermethrine, integrated pest management, Tea looper].AbstrakPenelitian telah dilakukan untuk mengembangkan bioinsektisida berbahan aktif B. thuringiensisdari isolat asli Indonesia. Eksplorasi B. thuringiensisdari contoh berupa tanah, daun, dan ulat dari kebun teh telah dilakukan demikian pula dari habitat lain seperti kebun kakao dan akasia.  Berdasarkan morfologi koloni diperoleh 10 isolat yang menunjukkan ciri-ciri koloni B. thuringiensis. Selanjutnyaisolat yang diperoleh diuji kemampuan pembentukan kristal protein dengan pewarnaan dan pengamatan mikroskop phase kontras dan menghasilkan 4 isolat yang mampu membentuk kristal protein. Selanjutnya keempat isolat yang diperoleh digunakan sebagai bahan aktif dalam formulasi bioinsektisida. Formula terbaik berdasarkan kriteria viabilitas bakteri adalah formula yang menggunakan bahan pembawa berupa white clay. Formula terbaik untuk bioinsektida cair berdasarkan kriteria viabilitas B. thuringiensisdan kejernihan bioinsektisida adalah menggunakan maltosesebagai osmoprotektan.Pada pengujian toksisitas isolat B. thuringiensisterhadap larvaulat jengkal(Hyposidra talaca)menunjukkan bahwa B. thuringiensishasil percobaan dapat mengen-dalikan larva ulat jengkalhingga 37,5%. Namun demikian toksisitasnya memerlukan waktu yang lebih lama dibandingkan dengan bioinsektisida berbahan aktif B. thuringiensiskomersial. [Kata kuci: Bioinsektisida Bt, pengendalian hama terpadu, sipermetrin, ulat jengkal teh].

scholarly journals Relationship between chemical properties of the soil and the occurrence of Bacillus thuringiensis

2009 ◽  
Vol 39 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Ricardo Antonio Polanczyk ◽  
José Cola Zanúncio ◽  
Sérgio Batista Alves
Keyword(s):  
Bacillus Thuringiensis ◽  
Integrated Pest Management ◽  
Pest Management ◽  
Soil Ph ◽  
Negative Correlation ◽  
Chemical Properties ◽  
High Positive Correlation ◽  
Positive Correlation ◽  
High Negative Correlation ◽  
The Relationship

The soil and other substrates such as mushroom compounds are the main sources of new Bacillus thuringiensis (Bt) isolates for Integrated Pest Management programs. This study describes the relationship between chemical properties of the soil (pH, OM, P3+, K1+, Ca2+, Mg2+, H1++Al3+, B3+, Cu2+, Fe2+, Mn2+ and Zn2+) and the occurrence of Bt in Brazil. A total of 1,197 bacterial colonies were obtained, being 512 of them identified as Bt. The Bt index (iBt), which is the relation between Bt colonies and bacterial counts ranged from 0.18 to 0.86. The iBt may be expressed with the formula: iBt= -0.4 + 0.6Ca2+ + 0.07Cu2+ + 0.009Fe2+ - 0.53Mg2+ -0.12Mn2+ + 1.26Zn2+. A cluster of samples with fewer colonies and a high negative correlation (antagonism) between Mn2+ and Ca2+; Mg2+ and Ca2+; Mg2+ and Zn2+; Mn2+ and Zn2+ and a high positive correlation (synergism) between Mn2+ and Mg2+; Zn2+ and Ca2+ was observed. The relationship between these elements and their effect on the Bt presence are discussed.

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