Resistance Management for Sustainable Use of Bacillus thuringiensis Crops in Integrated Pest Management

2004 ◽  
pp. 65-95 ◽  
Author(s):  
Y. Carrière ◽  
M. S. Sisterson ◽  
B. E. Tabashnik
2006 ◽  
Vol 20 (3) ◽  
pp. 756-767 ◽  
Author(s):  
Clarissa M. Hammond ◽  
Edward C. Luschei ◽  
Chris M. Boerboom ◽  
Pete J. Nowak

Agronomic research and extension personnel generally recognize the benefits of integrated pest management (IPM) but IPM practices have not been rapidly adopted by farmers. In order for applied research and extension programs to be as influential as possible, strategies and tactics must be evaluated in the context of the real-world constraints experienced by farmers. We investigated the linkage between farmers' pest management behaviors, attitudes, and constraints by analyzing an extensive corn pest management survey distributed throughout Wisconsin in 2002. Our objectives were to (1) create a benchmark against which future changes in pest management practices could be detected and (2) explore potential associations between practices and farm characteristics, e.g., farm size or commodity produced. A total of 213 farmers responded with descriptions of their operations; weed, insect, and disease pest management practices; crop consultant usage; interactions with their local agrichemical dealer; and attitudes regarding pest management decision-making. We compared the relative responses of cash-grain and dairy farmers as well as managers of large and small farms. Larger farm size and percentage of operation in cash-grain production were associated with an increased frequency of rotating crops, rotating herbicide families, and use of a broadcast herbicide application. Managers of large farms and/or cash-grain crops also more frequently indicated considering the level of pest control, price, carryover potential, weed resistance management, environmental safety, and risk to the applicator than did dairy or small-sized operations. Cash-grain farmers had significantly higher scores on a calculated IPM index than did dairy farmers (P < 0.0001). We also found a significant positive relationship between farm size and IPM score (P < 0.0001). Our results provide a benchmark for future comparisons of IPM adoption rates in Wisconsin and highlight the association between IPM research/extension and farmers' management behavior.


2007 ◽  
Vol 97 (2) ◽  
pp. 191-200 ◽  
Author(s):  
A. Khaliq ◽  
M.N.R. Attique ◽  
A.H. Sayyed

AbstractThe susceptibility of representative pyrethroid (cypermethrin, deltamethrin, lambdacyhalothrin, bifenthrin), organophosphate (chlorpyriphos, triazophos, profenophos) and new chemistry insecticides (spinosad, indoxacarb and emamectin) was investigated for 18 field populations ofPlutella xylostella(Linnaeus) from three different zones in Pakistan. The LC50(mg ml−1; 48 h) values of pyrethroids for various populations ranged from 0.19–1.88 for cypermethrin, 0.31–2.64 for deltamethrin, 0.08–1.16 for lambdacyhalothrin and 0.07–0.88 for bifenthrin. The LC50(mg ml−1; 48 h) of organophosphates ranged from 0.52–5.67 for chlorpyriphos, 0.37–4.14 for triazophos and 0.03–2.65 for profenophos. The most probable reason for low toxicity of organophosphates and pyrethroids is the evolution of multiple resistance mechanisms; however, further studies are required to establish these mechanisms. When these same products were tested against a susceptible laboratory population (Lab-Pak), the new chemistry compounds were significantly more toxic than pyrethroids and organophosphates. The results are discussed in relation to integrated pest management and insecticide resistance management strategies forP. xylostella.


Author(s):  
P.J. Cameron ◽  
G.P. Walker ◽  
T.J.B. Herman ◽  
J.D. Fletcher

Insects ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 316 ◽  
Author(s):  
Beatriz Dáder ◽  
Eduardo Aguirre ◽  
Primitivo Caballero ◽  
Pilar Medina

The joint use of baculoviruses and synthetic insecticides for integrated pest management requires the study of the additive, synergistic or antagonistic effects among them on pest mortality. Droplet bioassays were conducted with Autographa californica multiple nucleopolyhedrovirus (AcMNPV), Spodoptera littoralis nucleopolyhedrovirus (SpliNPV) and seven insecticides (azadirachtin, Bacillus thuringiensis, cyantraniliprole, emamectin, metaflumizone, methoxyfenozide and spinetoram) on Spodoptera exigua and Spodoptera littoralis. The lethal concentrations LC50 and LC95 were calculated through probit regressions. Then, the sequential feeding of insecticides and nucleopolyhedroviruses was studied. Larvae were provided with the LC50 of one insecticide, followed by the LC50 of one nucleopolyhedrovirus 24 h later. The inverse order was also conducted. The insecticide LC50 and LC95 were higher for S. littoralis than for S. exigua. AcMNPV showed greater toxicity on S. exigua than SpliNPV on S. littoralis. Emamectin showed synergy with AcMNPV when the chemical was applied first, and metaflumizone and AcMNPV were synergistic regardless of the order of application, both from the first day of evaluation. SpliNPV was synergistic with azadirachtin and emamectin when it was applied first, but synergy was reached after 12–13 days. Excellent control is possible with the LC50 of azadirachtin, emamectin and metaflumizone in combination with nucleopolyhedroviruses, and merits further study as a means of controlling lepidopteran pests.


2018 ◽  
Vol 85 (0) ◽  
Author(s):  
Gabriella Aparecida Salis de Carvalho ◽  
Daniel Júnior Martins ◽  
Isadora Mileny Costa de Brito ◽  
Sebastião Lourenço de Assis Júnior ◽  
Marcus Alvarenga Soares ◽  
...  

ABSTRACT: The entomopathogen Bacillus thuringiensis (Bt) is widely used as one of the ingredients in pest control formulations, but researches conducted on its effect on non-target organisms are still in the nascent stage. This investigation aimed to uncover if Bt treated with Tenebrio molitor (Coleoptera: Tenebrionidae) larvae and pupae could affect the biological variables of Podisus nigrispinus (Hemiptera: Pentatomidae) and Palmistichus elaeisis (Hymenoptera: Eulophidae), all of which established natural enemies of leaf defoliator caterpillars in the eucalyptus culture. Larvae of T. molitor were fed on wheat bran containing different concentrations of B. thuringiensis (0.00; 0.25; 0.50; 1.00; 2.00 and 4.00 g Agree/kg bran). When the larvae attained size of about 2 cm, they were used as prey for P. nigrispinus (Bioassay I), and their pupae used as hosts for P. elaeisis (Bioassay II). Only the biological variables oviposition period and egg numbers by posture of the predator P. nigrispinus were altered. The biological variables of P. elaeisis were not altered, since it was possible to use these control methods within the integrated pest management.


2019 ◽  
Vol 87 (1) ◽  
Author(s):  
Happy WIDIASTUTI ◽  
TRI - PANJI ◽  
Ciptadi Achmad YUSUP ◽  
Iman RUSMANA ◽  
Tri Eko WAHYONO

A study has been conducted to develop indigenousBacillusthuringiensisbioinsecticide. Preliminary study has been conducted to explore B. thuringiensisfrom sample of soil, leaf, and infected larvae from selected tea area as well as another area such as cocoa, and acasia. The result showed that based on the colony morphology, it was found 10 isolateswith the characteristics of B. thuringiensis colony.  Assessed of the ability to formed crystal using phase contrast microscope and staining revealed that 4 isolates as crystal protein forming isolates. The four isolates used as active coumpound of bioinsecticide. The best formula based on the viability of bacteria was the one withwhite clay as a carrier. While the best liquid formula based on the similar characteristic was using maltosa as osmoprotectant. Theassessment ofthetoxisity B. thuringiensistowards Hyposidra talacalarvae showed that B. thuringiensiscould control H. talacalarvaeup to 37.5%. However, the toxicity need longer periode compared to that commercial B. thuringiensisbioinsecticide. [Keywords:Bt insecticide, cypermethrine, integrated pest management, Tea looper].AbstrakPenelitian telah dilakukan untuk mengembangkan bioinsektisida berbahan aktif B. thuringiensisdari isolat asli Indonesia. Eksplorasi B. thuringiensisdari contoh berupa tanah, daun, dan ulat dari kebun teh telah dilakukan demikian pula dari habitat lain seperti kebun kakao dan akasia.  Berdasarkan morfologi koloni diperoleh 10 isolat yang menunjukkan ciri-ciri koloni B. thuringiensis. Selanjutnyaisolat yang diperoleh diuji kemampuan pembentukan kristal protein dengan pewarnaan dan pengamatan mikroskop phase kontras dan menghasilkan 4 isolat yang mampu membentuk kristal protein. Selanjutnya keempat isolat yang diperoleh digunakan sebagai bahan aktif dalam formulasi bioinsektisida. Formula terbaik berdasarkan kriteria viabilitas bakteri adalah formula yang menggunakan bahan pembawa berupa white clay. Formula terbaik untuk bioinsektida cair berdasarkan kriteria viabilitas B. thuringiensisdan kejernihan bioinsektisida adalah menggunakan maltosesebagai osmoprotektan.Pada pengujian toksisitas isolat B. thuringiensisterhadap larvaulat jengkal(Hyposidra talaca)menunjukkan bahwa B. thuringiensishasil percobaan dapat mengen-dalikan larva ulat jengkalhingga 37,5%. Namun demikian toksisitasnya memerlukan waktu yang lebih lama dibandingkan dengan bioinsektisida berbahan aktif B. thuringiensiskomersial. [Kata kuci: Bioinsektisida Bt, pengendalian hama terpadu, sipermetrin, ulat jengkal teh].


Agriculture ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 828
Author(s):  
Valborg Kvakkestad ◽  
Åsmund Lægreid Steiro ◽  
Arild Vatn

Integrated pest management (IPM) was introduced in the 1960s as a response to increasing pesticide use and has since evolved from being understood mainly as an economic issue to also including environmental and human health considerations. The EU has made IPM mandatory for all farmers through the Sustainable Use of Pesticides Directive (SUD). Using a mixed-methods approach, this paper examines how Norwegian cereal farmers have responded to this requirement. The qualitative results show that most farmers have an understanding of IPM that goes beyond economic considerations only. The quantitative results display that farmers’ intrinsic motivation for IPM changed after introduction of the SUD. There is increased emphasis on using methods other than spraying, producing grain without traces of pesticides, and preventing pesticide resistance. Farmers’ self-reported knowledge of IPM increased, and 41% of farmers stated that they use IPM to a greater extent than before the SUD was introduced. These results demonstrate that mandatory IPM requirements have been a successful strategy for increasing farmers use of IPM in Norway. Clearer IPM provisions and increased intrinsic motivation for IPM among farmers will, however, be important to reduce the risks from pesticides further.


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