Diversity of high-molecular-weight glutenin subunits and evaluation of genetic similarities in spring bread wheats from different breeding centers

Background.Glutenin is a storage protein in wheat seeds, important for the quality of bread prepared from wheat. Studying glutenin polymorphism can help to identify valuable genotypes and promising new breeding lines for further crossings. The aim of this study was to identify subunits of glutenin and determine alleles at the Glu-1loci in the spring bread wheat germplasm collection.Materials and methods.A panel of 54 Russian and 76 Kazakh bread wheat germplasm accessions from various breeding centers was selected. Gliadin electrophoresis was carried out in a concentrating and separating polyacrylamide gel system following the Laemmli method. Glutenin subunits were identified according to the catalogue produced by Payne and Lawrence.Results and discussion.At the Glu-A1 locus, two alleles, band c, were identified, with different frequencies of occurrence among studied wheat accessions. The Glu-A1ballele occurred more frequently than Glu-A1сin the studied germplasm from all breeding centers. Meanwhile, six alleles, а, b, c, d, fand g, were found at theGlu-B1 locus. The most frequently occurring Glu-B1с allele encoded two subunits (7+9). The third homologous gene, Glu-D1, had only two identified alleles, aand d, with various frequencies among the studied wheat accessions. Wheat germplasm of various origin had specific combinations of glutenin subunits, providing different scores of grain quality. For example, the combination of glutenin subunits, 2*, 7+9 and 5+10, provided the highest score (= 9) of grain quality. A single change in the Glu-1subunit composition, 2*, 7+9 and 2+12, caused a reduction in grain quality (= 7 score); and the combination of Null, 7and 5+10 was accompanied by the lowest (= 6) grain quality. The analysis showed that two loci, Glu-A1 and Glu-B1, induced significant differences between wheat accessions originated from Tyumen and Chelyabinsk, while the accessions from Chelyabinsk and East Kazakhstan differed significantly at the Glu-A1and Glu-D1loci.

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Genetic diversity of gliadin-coding alleles in bread wheat (Triticum aestivum L.) from Northern Kazakhstan

PeerJ ◽

10.7717/peerj.7082 ◽

2019 ◽

Vol 7 ◽

pp. e7082 ◽

Cited By ~ 1

Author(s):

Maral Utebayev ◽

Svetlana Dashkevich ◽

Nina Bome ◽

Kulpash Bulatova ◽

Yuri Shavrukov

Keyword(s):

Genetic Diversity ◽

Triticum Aestivum ◽

Bread Wheat ◽

Polyacrylamide Gel Electrophoresis ◽

Germplasm Collection ◽

Triticum Aestivum L ◽

Wheat Breeding ◽

Spring Bread Wheat ◽

Northern Kazakhstan

Background Spring bread wheat (Triticum aestivum L.) represents the main cereal crop in Northern Kazakhstan. The quality of wheat grain and flour strongly depends on the structure of gluten, comprised of gliadin and glutenin proteins. Electrophoresis spectra of gliadins are not altered by environmental conditions or plant growth, are easily reproducible and very useful for wheat germplasm identification in addition to DNA markers. Genetic polymorphism of two Gli loci encoding gliadins can be used for selection of preferable genotypes of wheat with high grain quality. Methods Polyacrylamide gel electrophoresis was used to analyse genetic diversity of gliadins in a germplasm collection of spring bread wheat from Northern Kazakhstan. Results The highest frequencies of gliadin alleles were found as follows, in Gli1: -A1f (39.3%), -B1e (71.9%), and -D1a (41.0%); and in Gli-2: -A2q (17.8%), -B2t (13.5%), and -D2q (20.4%). The combination of these alleles in a single genotype may be associated with higher quality of grain as well as better adaptation to the dry environment of Northern Kazakhstan; preferable for wheat breeding in locations with similar conditions.

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Grain quality in bread wheat lines T. aestivum with introgression of genetic material T. dicoccoides and T. dicoccum

Doklady of the National Academy of Sciences of Belarus ◽

10.29235/1561-8323-2018-62-6-712-718 ◽

2019 ◽

Vol 62 (6) ◽

pp. 712-718

Author(s):

O. A. Orlovskaya ◽

S. I. Vakula ◽

L. V. Khotyleva ◽

A. V. Kilchevsky

Keyword(s):

Bread Wheat ◽

Grain Quality ◽

Wheat Variety ◽

Genetic Material ◽

Introgression Lines ◽

Spring Bread Wheat ◽

Gluten Quality ◽

Gluten Content ◽

Wheat Lines

Related wild and cultural wheat species are regularly involved for expanding T. aestivum genetic diversity because they contain many valuable genes. We evaluated the effect of the genetic material of tetraploid species of the genus Triticum (T. dicoccoides, T. dicoccum) on the grain quality of introgression lines of spring bread wheat. The composition of the high molecular weight glutenin subunits which play an essential role in the formation of wheat baking properties was identified in the introgression lines of bread wheat and their parental forms. The traits of grain quality (hardness, protein and gluten content, gluten quality) were also evaluated. The lines with Glu-1 loci alleles from wheat relatives T. dicoccoides and Т. dicoccum were selected. It was found that the introgression of alien genetic material into the common wheat genome had a positive effect on the parameters of grain quality such as hardness, protein and gluten content. The lines with Glu-A1 loci alleles from T. dicoccoides and Glu-B1 from T. dicoccum were at the level of a parent wheat variety or of a higher gluten quality. As a result of the research, the new lines of bread soft wheat with high grain quality were found and can be used in the crop breeding.

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Identification of loci controlling the grain quality of spring bread wheat varieties of the nursery KASIB

Vestnik Omskogo gosudarstvennogo agrarnogo universiteta ◽

10.48136/2222-0364_2021_4_45 ◽

2021 ◽

pp. 45-52

Author(s):

I.V. POTOTSKAYA ◽

O.G. KUZMIN ◽

M.N. KOSHKIN ◽

V.P. SHAMANIN

Keyword(s):

Bread Wheat ◽

Grain Quality ◽

Spring Bread Wheat ◽

Wheat Varieties

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Response of Yield and Grain Quality of some Bread Wheat (Triticum aestivum L.) Genotypes to Different Sowing Dates

Journal of Plant Production ◽

10.21608/jpp.2016.46876 ◽

2016 ◽

Vol 7 (10) ◽

pp. 1043-1051

Author(s):

Magda Abd El-Rahman ◽

Aml El-Saidy

Keyword(s):

Triticum Aestivum ◽

Bread Wheat ◽

Grain Quality ◽

Triticum Aestivum L ◽

Sowing Dates

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High yielding spring bread wheat germplasm for global irrigated and rainfed production systems

Euphytica ◽

10.1007/s10681-006-9346-6 ◽

2007 ◽

Vol 157 (3) ◽

pp. 351-363 ◽

Cited By ~ 61

Author(s):

R. P. Singh ◽

J. Huerta-Espino ◽

R. Sharma ◽

A. K. Joshi ◽

R. Trethowan

Keyword(s):

Bread Wheat ◽

Production Systems ◽

Spring Bread Wheat ◽

Wheat Germplasm

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Analysis of HMW glutenin subunits encoded by chromosome 1A of bread wheat (Triticum aestivum L.) indicates quantitative effects on grain quality

Theoretical and Applied Genetics ◽

10.1007/bf00224285 ◽

1992 ◽

Vol 83 (3) ◽

pp. 373-378 ◽

Cited By ~ 167

Author(s):

N. G. Halford ◽

J. M. Field ◽

H. Blair ◽

P. Urwin ◽

K. Moore ◽

...

Keyword(s):

Triticum Aestivum ◽

Bread Wheat ◽

Grain Quality ◽

Triticum Aestivum L ◽

Glutenin Subunits ◽

Hmw Glutenin Subunits ◽

Hmw Glutenin

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Grain quality of drought tolerant accessions within the MRI Zemun Polje maize germplasm collection

Spanish Journal of Agricultural Research ◽

10.5424/sjar/2014121-4392 ◽

2014 ◽

Vol 12 (1) ◽

pp. 186 ◽

Cited By ~ 4

Author(s):

Jelena Vančetović ◽

Dragana Ignjatović-Micić ◽

Sofija Božinović ◽

Milosav Babić ◽

Milomir Filipović ◽

...

Keyword(s):

Grain Quality ◽

Germplasm Collection ◽

Maize Germplasm ◽

Drought Tolerant

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Grain quality of common wheat lines with T. kiharae genetic material

Faktori eksperimental'noi evolucii organizmiv ◽

10.7124/feeo.v23.998 ◽

2018 ◽

Vol 23 ◽

pp. 108-113

Author(s):

O. A. Orlovskaya ◽

S. I. Vakula ◽

L. V. Khotyleva ◽

A. V. Kilchevsky

Keyword(s):

Molecular Weight ◽

Common Wheat ◽

Grain Quality ◽

Genetic Material ◽

Glutenin Subunits ◽

Gluten Quality ◽

Gluten Content ◽

Sds Page ◽

Wheat Lines

Aim. T. kiharae (AtAtGGDD, 2n=42) is a source of high protein and gluten content, resistance to many diseases. Сommon wheat lines with the introgression of T. kiharae genetic material were obtained in order to enrich T. aestivum L. gene pool. The aim of this study was to assess the impact of T. kiharae genetic material on the grain quality of T. aestivum/T. kiharae introgression lines. Methods. The composition of the high molecular weight glutenin subunits was analyzed by SDS-PAGE. Evaluation of the most important traits of grain quality (hardness, protein and gluten content, gluten quality) was carried out according to GOST. Results. Сomparative analysis of the composition of high molecular weight glutenin subunits of introgressive lines and their parental forms allowed us to identify lines with novel alleles of Glu-1 loci, specific for T. kiharae. For most of the introgression lines T. aestivum/T. kiharae hardness, protein and gluten content were higher than for parent wheat varieties. Conclusions. Introgression of T. kiharaegenetic material in the genome of common wheat had a positive effect on all studied parameters of grain quality except the gluten quality. Keywords: common wheat, T. kiharae, glutenin, SDS-PAGE, quality of grain.

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Economic and biological characteristics of the spring bread wheat varieties developed in the Voronezh FASC named after V. V. Dokuchaev

Grain Economy of Russia ◽

10.31367/2079-8725-2021-73-1-31-38 ◽

2021 ◽

Vol 1 (1) ◽

pp. 31-38

Author(s):

E. I. Malokostova

Keyword(s):

Environmental Factors ◽

Bread Wheat ◽

Grain Quality ◽

Seeding Rate ◽

Spring Bread Wheat ◽

Wheat Varieties ◽

Potential Productivity ◽

High Productivity ◽

Study Results ◽

Productivity Potential

The current paper has discussed information on the characteristics and properties of the zoned spring bread wheat varieties developed in the Federal Agricultural Scientific Centre named after V.V. Dokuchaev. The purpose of the study was to develop new spring bread wheat varieties with high productivity and grain quality, resistant to unfavorable biotic and abiotic environmental factors and adapted to the conditions of the Central Blackearth Region. In the FASC named after V.V. Dokuchaev there were developed such highly productive spring bread wheat varieties as ‘Krestyanka’, ‘Kurskaya 2038’, ‘Voronezhskaya 12’, ‘Chernozemnouralskaya 2’ and ‘Voronezhskaya 18’, which are currently approved for use in production. There were analyzed the study results of productivity, grain quality and resistance to unfavorable environmental factors in different growing periods of the varieties. There was found an unambiguous productivity excess of the developed varieties over the standard ones. The endurance of the presented varieties in unfavorable conditions indicates an increase in the stability of the main traits that characterize productivity, resistance to drought, diseases, and lodging. There has been established, that in terms of grain quality the varieties ‘Krestyanka’ and ‘Chernozemnouralskaya 2’ belong to strong wheat with potential productivity of 7.20 and 6.65 t/ha, respectively. The varieties ‘Kurskaya 2038’, ‘Voronezhskaya 12’ and ‘Voronezhskaya 18’ are valuable wheat varieties with realized productivity potential of 5.31, 5.70 and 6.96 t/ha, respectively. There has been identified that the varieties ‘Chernozemnouralskaya 2’ and ‘Voronezhskaya 18’ are recommended to sow with a seeding rate of 6.0 million germinating grains per hectare for the rapid seed reproduction and obtaining high gross grain yield, since productivity, yield of conditioned seeds, germination and 1000-grain weight at this rate was the best. The sowing dates of these varieties were early.

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