SEARCH FOR RESISTANCE SOURCES TO GLOBODERA PALLIDA AND POTATO VIRUS X IN THE COLLECTION OF POTATO VARIETIES USING MOLECULAR MARKERS

The genotypes of potatoes bred by Falenki Breeding station were studied for the presence of resistance genes markers to the following pathogens: Globodera rostochiensis, Globodera pallidа, Synchytrium endobioticum, potato virus X (PVХ) and potato virus Y (PVY). The method of multiplex PCR analysis was used. The varieties Shurminsky 2, Alisa, Viza, Chayka, Ognivo, Darik, Gloriya, Golubka, Virazh and a promising variety sample 56-09 were studied. In most (8 out of 10) genotypes, marker linked to the Sen1 gene of resistance to S. endobioticum was identified. DNA marker of the G. rostochiensis resistance gene (H1) and the G. pallida resistance gene marker (Gpa2) were found in six genotypes. The marker of the PVX resistance gene (Rx1) was detected in the varieties Shurminsky 2, Alisa, Chayka, Golubka, and Virazh. It has been established that none of the studied potato genotypes carries markers RYSC3, Ry186, YES3-3A linked to the PVY resistance genes. Although in the field, resistance was detected in the samples Chayka, Darik, Virazh, Alisa. Molecular markers linked to the largest number of resistance genes studied (H1, Gpa2, Sen1, and Rx1) were identified in the varieties Shurminsky 2, Golubka, and Virazh. Among the DNA markers used in the work, the data of potato genotype assessment using markers of virus resistance genes (PVX, RYSC3, Ry186, YES3-3A) were less consistent with field observations. The use of molecular markers makes it possible to determine the presence of resistance genes and assess the prospects of a sample in a short period of time, but, at the same time, requires careful choice of a DNA marker that is highly correlated with the manifestation of the trait.

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Presence and distribution of economically important potato viruses in Montenegro

Pesticidi i fitomedicina ◽

10.2298/pif1102117z ◽

2011 ◽

Vol 26 (2) ◽

pp. 117-127

Author(s):

Jelena Zindovic

Keyword(s):

Potato Virus ◽

Leaf Roll ◽

Potato Virus X ◽

Enzyme Linked Immunosorbent Assay ◽

Potato Leaf ◽

Potato Virus S ◽

Potato Varieties ◽

Potato Viruses ◽

Das Elisa

The research was carried out, in the period 2002-2004 in order to determine the presence and distribution of potato viruses at 12 different locations and on 9 different potato varieties grown in Montenegro. The research included collecting of samples in seed potato crops and testing of six economically important potato viruses: Potato leaf roll virus (PLRV), Potato virus Y (PVY), Potato virus X (PVX), Potato virus S (PVS), Potato virus A (PVA) i Potato virus M (PVM). Using the direct enzyme-linked immunosorbent assay (DAS-ELISA) and commercial antisera specific for six potato viruses, it was found that PVY was the most frequent virus during the three-year research period. The second frequent virus was PVS, followed by PVA, PLRV, PVM and PVX. Single and mixed infections were detected, and the most prevalent were the single infections of PVY. Also, in the period 2002-2004, PVY had the highest distribution and the number of present viruses was different at different localities and on different potato varieties. Further investigations were related to detailed characterization of the most prevalent virus (PVY), which is at the same time economically the most important one. Serological characterization of PVY was performed utilizing DAS-ELISA kit with commercial monoclonal antibodies specific for detection of the three strain groups of PVY, and the two strain groups - necrotic (PVYN/PVYNTN) and common (PVYO), were identified. Necrotic strains were prevalent in 2002 and 2004, while in 2003 PVYO was the most frequent strain in virus population. The presence of stipple streak strain (PVYC) was not detected in any of the tested samples.

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Field Evaluation and Enzyme-Linked Immunosorbent Assay Detection of Potato Leaf Roll Virus, Potato Virus X and Potato Virus Y in Potato Germplasm

Journal of Agricultural Science ◽

10.5539/jas.v9n7p229 ◽

2017 ◽

Vol 9 (7) ◽

pp. 229 ◽

Cited By ~ 1

Author(s):

Romana Anjum ◽

M. Aslam Khan ◽

Kolawole Oluwaseun Olawale ◽

Raheel Baber

Keyword(s):

Immunosorbent Assay ◽

Potato Virus ◽

Potato Virus Y ◽

Rating Scale ◽

Leaf Roll ◽

Potato Virus X ◽

Potato Leaf Roll Virus ◽

Enzyme Linked Immunosorbent Assay ◽

Potato Leaf ◽

Potato Varieties

Polerovirus: potato leaf roll virus (PLRV), Potyvirus: potato virus Y (PVY) and Potexvirus: potato virus X (PVX) is more destructive and well distributed throughout the Pakistan. Incidence has been reported to be as high as 90%, 25%, and ≥ 15%, respectively in the potato growing regions. To find out the source of resistance, twenty-nine virus free potato varieties were grown under field conditions with good agricultural practices. The disease severity of PLRV, PVY and PVX was recorded to determine the level of resistance of the potato varieties according to the disease rating scale. Infectivity and biological assay of all twenty-nine varieties were done in green house on potato, Datura stramonium, Nicotiana glutinosa and Physalis floridana. Non-inoculated plants were served as control. Leaf samples from potato varieties were collected for serological detection of PLRV, PVY and PVX by Double antibody sandwich Enzyme-Linked Immunosorbent Assay (DAS-ELISA). Out of twenty nine varieties, none of the variety was resistant to PLRV although three varieties; Mirrato, 394021-120 and Orla were moderately resistant. Only FD 48-4 and TPS 9813 showed resistance to PVX and PVY. While FD 3-10, FD 9616 and FD 37-13 were moderately to PVX and PVY. Rest of the varieties was found susceptible to all three viruses.

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Comparative rates of movement of potato virus X into tubers and eyes of three potato varieties

American Potato Journal ◽

10.1007/bf02852740 ◽

1959 ◽

Vol 36 (11) ◽

pp. 410-413 ◽

Cited By ~ 6

Author(s):

J. P. MacKinnon ◽

James Munro

Keyword(s):

Potato Virus ◽

Potato Virus X ◽

Potato Varieties

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Disease Severity Index as It Affects Responses in Potato Virus X–Challenged Potato Varieties

International Journal of Vegetable Science ◽

10.1080/19315260.2015.1077914 ◽

2016 ◽

Vol 22 (5) ◽

pp. 471-479

Author(s):

Misbah Iqbal Qamar ◽

Yasir Iftikhar ◽

Aamir Ali ◽

Muhammad Irfan Ullah ◽

Mustansar Mubeen

Keyword(s):

Disease Severity ◽

Potato Virus ◽

Potato Virus X ◽

Severity Index ◽

Disease Severity Index ◽

Potato Varieties

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Tight Physical Linkage of the Nematode Resistance Gene Gpa2 and the Virus Resistance Gene Rx on a Single Segment Introgressed from the Wild Species Solanum tuberosum subsp. andigena CPC 1673 into Cultivated Potato

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi.1999.12.3.197 ◽

1999 ◽

Vol 12 (3) ◽

pp. 197-206 ◽

Cited By ~ 57

Author(s):

Jeroen Rouppe van der Voort ◽

Konstantin Kanyuka ◽

Edwin van der Vossen ◽

Abdelhafid Bendahmane ◽

Paul Mooijman ◽

...

Keyword(s):

Solanum Tuberosum ◽

Resistance Gene ◽

Potato Virus ◽

Wild Species ◽

Potato Virus X ◽

Globodera Pallida ◽

Cyst Nematodes ◽

Total Size ◽

Bac Clones ◽

Mapping Populations

Comparative mapping studies within several plant genera have shown that genes for resistance to taxonomically diverse pathogens are often co-localized on the maps of these genotypes. Here we describe the genetic analysis of such a gene cluster on chromosome 12 of potato. This cluster harbors the resistance loci Gpa2 and Rx, which confer resistance to the potato cyst nematode Globodera pallida and potato virus X (PVX), respectively. By screening two mapping populations with 10 CAPS (cleaved amplified polymorphic sequence) markers derived from AFLP (amplified fragment length polymorphism) markers and the ends of overlapping bacterial artificial chromosome (BAC) clones, it is demonstrated that Gpa2 and Rx are tightly linked. From a recombination fraction of 1/3386, found after screening two different mapping populations, a genetic distance of 0.02 cM between Gpa2 and Rx was calculated. The physical length of this genetic interval does not exceed 300 kb of DNA as determined by the total size of overlapping BAC clones in the Rx/Gpa2 contig. Evidence is provided that both genes have been introgressed from a single wild species clone, Solanum tuberosum subsp. andigena CPC 1673. This suggests that these genes underlying the disease resistance specificities to potato virus X and potato cyst nematodes have a common evolutionary origin.

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