Improvement of the technology of bovine embryos transfer in the FSBSI "Center of experimental embryology and reproductive biotechnologies"

Abstract The efficiency of somatic cell cloning (somatic cell nuclear transfer; SCNT) as well as in vitro fertilization/in vitro embryo production (IVF/IVP) in mammals stay at relatively same level for over a decade. Despite plenty of different approaches none satisfactory break-through took place. In this article, we briefly summarize the implementation of mesenchymal stem cells (MSCs) for experimental embryology. The advantages of using MSCs as nuclear donors in somatic cell cloning and in vitro embryo culture are described. The description of results obtained with these cells in mammalian embryo genomic engineering is presented.

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Faculty Opinions recommendation of Investigation of respiration of individual bovine embryos produced in vivo and in vitro and correlation with viability following transfer.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.715897924.791352833 ◽

2012 ◽

Author(s):

Magdalena Zernicka-Goetz ◽

Anna Ajduk

Keyword(s):

Bovine Embryos

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Evaluation of Bovine Embryo Biopsy Techniques according to Their Ability to Preserve Embryo Viability

Journal of Biomedicine and Biotechnology ◽

10.1155/2012/541384 ◽

2012 ◽

Vol 2012 ◽

pp. 1-5 ◽

Cited By ~ 13

Author(s):

M. Cenariu ◽

E. Pall ◽

C. Cernea ◽

I. Groza

Keyword(s):

Pregnancy Rate ◽

Genetic Diagnosis ◽

Needle Aspiration ◽

Embryo Biopsy ◽

Embryo Cryopreservation ◽

Bovine Embryo ◽

Bovine Embryos ◽

Embryo Viability ◽

Biopsy Methods ◽

Biopsy Method

The purpose of this research was to evaluate three embryo biopsy techniques used for preimplantation genetic diagnosis (PGD) in cattle and to recommend the least invasive one for current use, especially when PGD is followed by embryo cryopreservation. Three hundred bovine embryos were biopsied by either one of the needle, aspiration or microblade method, and then checked for viability by freezing/thawing and transplantation to recipient cows. The number of pregnancies obtained after the transfer of biopsied frozen/thawed embryos was assessed 30 days later using ultrasounds. The results were significantly different between the three biopsy methods: the pregnancy rate was of 57% in cows that received embryos biopsied by needle, 43% in cows that received embryos biopsied by aspiration, and 31% in cows that received embryos biopsied by microblade. Choosing an adequate biopsy method is therefore of great importance in embryos that will undergo subsequent cryopreservation, as it significantly influences their viability after thawing.

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Contrasting effects of heat shock during in vitro maturation on development of in vitro‐fertilized and parthenogenetic bovine embryos

Reproduction in Domestic Animals ◽

10.1111/rda.13544 ◽

2019 ◽

Vol 54 (10) ◽

pp. 1357-1365

Author(s):

Luiz Sergio Almeida Camargo ◽

Fernanda Queiros Costa ◽

Michele Munk ◽

Sabine Wohlres‐Viana ◽

Raquel Varela Serapião ◽

...

Keyword(s):

Heat Shock ◽

In Vitro Maturation ◽

Bovine Embryos

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Microinjection of Bovine Embryos with a Foreign Gene and Its Detection at the Blastocyst Stage

Journal of Dairy Science ◽

10.3168/jds.s0022-0302(93)77677-8 ◽

1993 ◽

Vol 76 (11) ◽

pp. 3392-3399 ◽

Cited By ~ 17

Author(s):

Esmail Behboodi ◽

G.B. Anderson ◽

S. Horvat ◽

J.F. Medrano ◽

J.D. Murray ◽

...

Keyword(s):

Blastocyst Stage ◽

Foreign Gene ◽

Bovine Embryos

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Sex Determination of Bovine Embryos Using H-Y Antibodies

Acta Veterinaria Scandinavica ◽

10.1186/bf03548052 ◽

1989 ◽

Vol 30 (2) ◽

pp. 155-164 ◽

Cited By ~ 1

Author(s):

Birthe Avery ◽

Mette Schmidt

Keyword(s):

Sex Determination ◽

Bovine Embryos

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Nobiletin enhances the development and quality of bovine embryos in vitro during two key periods of embryonic genome activation

Scientific Reports ◽

10.1038/s41598-021-91158-7 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Karina Cañón-Beltrán ◽

Yulia N. Cajas ◽

Serafín Peréz-Cerezales ◽

Claudia L. V. Leal ◽

Ekaitz Agirregoitia ◽

...

Keyword(s):

Embryonic Development ◽

Signaling Pathway ◽

Akt Signaling ◽

Mitochondrial Activity ◽

Bovine Embryos ◽

Akt Signaling Pathway ◽

Cell Stage ◽

Development In Vitro

AbstractIn vitro culture can alter the development and quality of bovine embryos. Therefore, we aimed to evaluate whether nobiletin supplementation during EGA improves embryonic development and blastocyst quality and if it affects PI3K/AKT signaling pathway. In vitro zygotes were cultured in SOF + 5% FCS (Control) or supplemented with 5, 10 or 25 µM nobiletin (Nob5, Nob10, Nob25) or with 0.03% dimethyl-sulfoxide (CDMSO) during minor (2 to 8-cell stage; MNEGA) or major (8 to 16-cell stage; MJEGA) EGA phase. Blastocyst yield on Day 8 was higher in Nob5 (42.7 ± 1.0%) and Nob10 (44.4 ± 1.3%) for MNEGA phase and in Nob10 (61.0 ± 0.8%) for MJEGA phase compared to other groups. Mitochondrial activity was higher and lipid content was reduced in blastocysts produced with nobiletin, irrespective of EGA phase. The mRNA abundance of CDK2, H3-3B, H3-3A, GPX1, NFE2L2 and PPARα transcripts was increased in 8-cells, 16-cells and blastocysts from nobiletin groups. Immunofluorescence analysis revealed immunoreactive proteins for p-AKT forms (Thr308 and Ser473) in bovine blastocysts produced with nobiletin. In conclusion, nobiletin supplementation during EGA has a positive effect on preimplantation bovine embryonic development in vitro and corroborates on the quality improvement of the produced blastocysts which could be modulated by the activation of AKT signaling pathway.

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