scholarly journals ENRAIZAMENTO IN VITRO DE CULTIVARES DE Gerbera hybrida (ASTERACEAE)

2020 ◽  
Vol 22 (1) ◽  
pp. 125-139
Author(s):  
Tarcisio Rangel do Couto ◽  
João Sebastião de Paula Araujo ◽  
Leandro Miranda de Almeida ◽  
João Paulo de Lima Aguilar
Keyword(s):  
Gerbera Hybrida

O trabalho objetivou otimizar um protocolo de enraizamento in vitro durante a micropropagação de cultivares de gérbera. Foram testadas três cultivares de gérbera (‘Pacific’, ‘Igloo’, ‘Igor’) em DIC fatorial 3x2x2, sendo três concentrações de ANA (0; 2,68 e 5,36 µmol L-1), duas concentrações de sais do meio MS (50% e 100%) e duas concentrações de sacarose (15 e 30 g L-1), com 10 repetições e a unidade experimental um frasco com 30 mL de meio e cinco plantas. Foi utilizado o meio MS, contendo 7,5 g L-1 de ágar e pH ajustado para 5,8. Após 30 dias foram contabilizados o número de folhas, o número de raízes e a massa fresca das mudas. O tratamento com 5,36 µmol L-1 de ANA + 100% de MS + 30 g L-1 de sacarose resultou em maior massa fresca, entretanto as mudas apresentaram-se com massas de calos na base, raízes mais espessas e em menor número. Provavelmente, o que resultou em maior ganho de biomassa, porém de tamanho menor que as mudas dos outros tratamentos. Recomenda-se para enraizamento in vitro de gérberas, meio MS completo com 30 g L-1 de sacarose e ausência de ANA.

Effect of Light-emitting Diodes on Growth of in vitro Propagules in Gerbera hybrida

2012 ◽  
Vol 20 (4) ◽  
pp. 233-237 ◽  
Author(s):  
Min-Kyung Kwon ◽  
◽  
Hyo Hoon Nam ◽  
Jeon Joong Sung ◽  
Jea Ha Lim
Keyword(s):  
Light Emitting Diodes ◽  
Gerbera Hybrida ◽  
Light Emitting ◽  
Effect Of Light

Mineral nutrition and in vitro growth of Gerbera hybrida (Asteraceae)

2014 ◽  
Vol 50 (4) ◽  
pp. 458-470 ◽  
Author(s):  
Randall P. Niedz ◽  
Scott E. Hyndman ◽  
Terrence J. Evens ◽  
Albert A. Weathersbee
Keyword(s):  
Mineral Nutrition ◽  
In Vitro Growth ◽  
Gerbera Hybrida

scholarly journals Genetic engineering of apple (Malus domestica Borkh.) for resistance to fungal diseases using g2ps1 gene from Gerbera hybrida (Asteraceae)

2014 ◽  
Vol 20 (1-2) ◽  
Author(s):  
N. M. Ali Bacha ◽  
M. Batha ◽  
A. M. Abdul-Kader
Keyword(s):  
Genetic Engineering ◽  
Shoot Multiplication ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Fungal Resistance ◽  
Pcr Analysis ◽  
Gerbera Hybrida ◽  
Adventitious Shoot Formation ◽  
Selection Agent

In the present study, g2ps1 gene from Gerbera hybrida coding for 2-pyrone synthase which contribute for fungal and insect resistance was used. The aim was to work out an efficient approach of genetic transformation for apple cvs. ‘Golden Delicious’, ‘Royal Gala’ and ‘MM111’, ‘M26’ rootstocks for improving their fungal resistance using genetic engineering techniques. Adventitious shoot formation from leaf pieces of apples studied was achieved using middle leaf segments taken from the youngest leaves from in vitro-grown plants.Optimum conditions for ‚direct’ shoot organogenesis resulted in high regeneration efficiency of  0%, 95%, 92%, 94% in the studied apples respectively. Putative transgenic shoots could be obtained on MS media with B5 Vitamins, 5.0 mg l-1 BAP, or 2.0 mg l-1 TDZ with 0.2 mg l-1 NAA in the presence of the selection agent “PPT” at 3.0-5.0 mgl-1. Shoot multiplication of transgenic shoots was achieved on: MS + B5 vitamins + 1.0 mg l-1 BAP + 0.3 mg l-1 IBA, 0.2 mg l-1 GA3+1.0 g/l MES+ 30 g/l sucrose + 7.0 g/l Agar, with the selection agent PPT at 5.0 mg l-1 and were subcultured every 4 weeks in order to get sufficient material to confirm transformation of the putative shoots obtained. Six, seven, one and six transgenic clones of the apples studied respectively have been obtained and confirmed by selection on the media containing the selection agent “PPT” and by PCR analysis using the suitable primers in all clones obtained for the presence of the selection” bar gene (447 bp) and the gene-of- interest “g2PS1” (1244 bp), with transformation efficiency of 0.4%, 0.6%, 0.1% and 0.3% respectively. These transgenic clones were multiplied further in vitro in the presence of the selection agent ‘PPT’ and rooted in vitro. Rooted transgenic plantlets were successfully acclimatized and are being kept under-containment conditions according to the biosafety by-law in Syria to evaluate their performance for fungal resistance .

scholarly journals Effect of Three Types of Ion Beam Irradiation on Gerbera (Gerbera hybrida) In Vitro Shoots with Mutagenesis Efficiency

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1480
Author(s):  
Tomoya Hosoguchi ◽  
Yuna Uchiyama ◽  
Hinata Komazawa ◽  
Masaki Yahata ◽  
Takashi Shimokawa ◽  
...  
Keyword(s):  
Ion Beam ◽  
Ion Beams ◽  
Beam Irradiation ◽  
Ion Beam Irradiation ◽  
Gerbera Hybrida ◽  
Line Energy ◽  
In Vitro Shoots ◽  
Genomic Mutation ◽  
Morphological Mutation

Gerbera in vitro shoots were irradiated using three types of ion beams with different line energy transfers (LETs) to investigate the effective LET and absorbed doses for mutagenesis. Furthermore, genomic mutation analyses were conducted on the obtained mutants. Survival rate analysis showed a lower lethal dose 50% (LD50) with ion beams with higher LETs. Trait/morphological mutations exhibited changes in the color and shape of petals and male sterility. Irradiation conditions with the highest growth change and trait/morphological mutation rates in each ion were C irradiation at 10 Gy, Ar irradiation at 5 Gy, and Fe irradiation at 5 Gy, with a range of absorbed dose of around LD50 to about 10 Gy lower. The highest trait/morphological mutation rate was 14.1% with Ar irradiation at 5 Gy, which was one of the criteria for ion beam irradiation of gerbera in vitro shoots. Furthermore, the genomic mutation in the flower color, petal shape, and male sterile mutants were confirmed by genotype analysis using Genotyping by Random Amplicon Sequencing-Direct technology. This is the first study to report the efficient production of gerbera mutants that could be analyzed. Our findings may lead to more efficient gerbera mutant production and analysis technology.

scholarly journals In Vitro Formation of Gerbera (Gerbera hybrida Hort.) Plantlets through Excised Scape Culture

1983 ◽  
Vol 52 (1) ◽  
pp. 45-50 ◽  
Author(s):  
Chien-young CHU ◽  
Min-chang HUANG
Keyword(s):  
Gerbera Hybrida

Ultrastructural Investigations of the Contractile Filaments of Amoebae

1974 ◽  
Vol 32 ◽  
pp. 188-189
Author(s):  
P.L. Moore
Keyword(s):  
Cytoplasmic Streaming ◽  
Three Dimensional ◽  
Freeze Fracture ◽  
Amoeboid Movement ◽  
Different Types ◽  
Chemical Conditions ◽  
Complete Interpretation

Previous freeze fracture results on the intact giant, amoeba Chaos carolinensis indicated the presence of a fibrillar arrangement of filaments within the cytoplasm. A complete interpretation of the three dimensional ultrastructure of these structures, and their possible role in amoeboid movement was not possible, since comparable results could not be obtained with conventional fixation of intact amoebae. Progress in interpreting the freeze fracture images of amoebae required a more thorough understanding of the different types of filaments present in amoebae, and of the ways in which they could be organized while remaining functional.The recent development of a calcium sensitive, demembranated, amoeboid model of Chaos carolinensis has made it possible to achieve a better understanding of such functional arrangements of amoeboid filaments. In these models the motility of demembranated cytoplasm can be controlled in vitro, and the chemical conditions necessary for contractility, and cytoplasmic streaming can be investigated. It is clear from these studies that “fibrils” exist in amoeboid models, and that they are capable of contracting along their length under conditions similar to those which cause contraction in vertebrate muscles.

In Vitro Induction of Crystals of MT Protein by Vinblastine-Colcemid in Mouse Spermatids

1974 ◽  
Vol 32 ◽  
pp. 132-133
Author(s):  
John J. Wolosewick ◽  
John H. D. Bryan
Keyword(s):  
Endoplasmic Reticulum ◽  
Smooth Endoplasmic Reticulum ◽  
Nuclear Ring ◽  
Rough Er ◽  
Distal Direction ◽  
In Vitro Induction

Early in spermiogenesis the manchette is rapidly assembled in a distal direction from the nuclear-ring-densities. The association of vesicles of smooth endoplasmic reticulum (SER) and the manchette microtubules (MTS) has been reported. In the mouse, osmophilic densities at the distal ends of the manchette are the organizing centers (MTOCS), and are associated with the SER. Rapid MT assembly and the lack of rough ER suggests that there is an existing pool of MT protein. Colcemid potentiates the reaction of vinblastine with tubulin and was used in this investigation to detect this protein.

Induction and Differentiation of Dental Epithelium in Vivo and in Vitro

1982 ◽  
Vol 40 ◽  
pp. 142-145
Author(s):  
E. J. Kollar
Keyword(s):  
Connective Tissue ◽  
Oral Mucosa ◽  
Basal Lamina ◽  
Functional Derivatives ◽  
Specific Source ◽  
Dental Epithelium ◽  
Connective Tissue Stroma

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.

Immunoenzymatic demonstration of the simultaneous presence of transferrin, hemopexin and albumin in a same hepatocyte and their ultrastructural localization

1978 ◽  
Vol 36 (2) ◽  
pp. 88-89
Author(s):  
M. Kraemer ◽  
J. Foucrier ◽  
J. Vassy ◽  
M.T. Chalumeau
Keyword(s):  
Plasma Proteins ◽  
Rat Hepatocytes ◽  
Ultrastructural Localization ◽  
Carrier Proteins ◽  
Normal Cells ◽  
Adult Rat ◽  
Adult Rat Hepatocytes ◽  
Monospecific Antisera ◽  
Different Levels

Some authors using immunofluorescent techniques had already suggested that some hepatocytes are able to synthetize several plasma proteins. In vitro studies on normal cells or on cells issued of murine hepatomas raise the same conclusion. These works could be indications of an hepatocyte functionnal non-specialization, meanwhile the authors never give direct topographic proofs suitable with this hypothesis.The use of immunoenzymatic techniques after obtention of monospecific antisera had seemed to us useful to bring forward a better knowledge of this problem. We have studied three carrier proteins (transferrin = Tf, hemopexin = Hx, albumin = Alb) operating at different levels in iron metabolism by demonstrating and localizing the adult rat hepatocytes involved in their synthesis.Immunological, histological and ultrastructural methods have been described in a previous work.

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