scholarly journals Content of sialic acids, ATP and superoxide anion-radical in loach embryos under the influence of flurenizyd

Author(s):  
N. Bodnarchuk ◽  
◽  
N. Harasym ◽  
A. Tril ◽  
D. Sanagurskiy ◽  
...  

It is known that flurenizide is a newly synthesized drug with pronounced antimicrobial, immunomodulatory functions. However, its effect on the functional properties of cells, in particular germ cells, is unknown. The aim of this study was to determine the presence of sialic acids as the final components of glycoconjugates, the main macroergic compound - ATP, to evaluate the generation of O2¯ in embryos of lobster (Misgurnus fossilis L.) under the influence of the antibiotic flurenizide. Studies have been performed on embryos of Misgurnus fossilis L. After fertilization, the zygotes were placed in Petri dishes with solutions of flurenizide in concentrations of 0.01; 0.05; 0.15; 1.0; 5.0; 15.0 mM, where allowed to develop. At the development stages, 2 blastomeres, 16 blastomeres, 64 blastomeres, VIII (256 blastomeres) and X divisions (1024 blastomeres) were sampled. In parallel, control studies were performed, where flureniside was not added to the samples. The content of sialic acids, ATP, superoxide anion radical was determined in the selected samples. We found that flureniside at the lowest concentration of 0.01 mM does not cause changes in the content of superoxide anion radical during early embryogenesis. The antibiotic in the maximum concentration causes changes in the content of free radical from the stage of development of 16 blastomeres to 1024 blastomeres, and from the stage of 64 blastomeres there is an increase in its number. Probably flurenizide at a concentration of 15.0 mM is the most reactive. It is known that flurenizide has antioxidant properties, but in its structure there are groups that have a toxic effect, which is most pronounced when exposed to high concentrations. In general, at the stage of development of 16 blastomeres there is a decrease in the amount of О2¯ under the influence of the studied antibiotic. At this time, the content of this free radical in the control increases, compared with other stages of development. Flureniside leads to an increase in the content of superoxide anion radical at development stage 2, 64 blastomeres and is particularly pronounced at concentrations from 0.05 to 15.0 mM at development stages 256 and 1024 blastomeres. 1024 blastomeres are the 10th stage of separation, where desynchronization occurs and the mitotic index decreases. Probably, the changes that take place at this stage of development are related to this. The antibiotic causes an increase in the content of sialic acids in the first stage of crushing (2 blastomeres). In step 16 of the blastomere, flureniside in low concentrations leads to a decrease in the content of sialic acids. However, already at the stage of separation of 64 and 256 blastomeres, the test substance in all concentrations causes a predominant decrease in the amount of sialic acids. At the last stage of synchronous crushing (1024 blastomeres) flurenizide in the maximum investigated concentration (15.0 mM) causes a significant accumulation of sialic acid content. Flureniside at concentrations of 0.15, 5.0 and 15.0 mМ causes a decrease in ATP content in loach embryos at the stage of development of 256 blastomeres by 28, 67 and 38 %, respectively. An increase in ATP content by 33 % occurs under the influence of flurenizide at a concentration of 1.0 mM. The ATP content also increases at the stage of development of embryos of 1024 blastomeres under the influence of flurenizide of all studied concentrations. Analysis of variance has shown that a significant contribution to the growth of ATP, sialic acid and superoxide anion radical, during the early embryogenesis of lobster embryos, is made by the factor of embryo development, while the factor of flureniside has a smaller contribution. Therefore, studies have shown that flurenizide causes increased generation of superoxide anion radical, decreased sialic acid content, as well as changes in ATP content in embryonic cells during early embryogenesis.

2015 ◽  
Vol 22 (37) ◽  
pp. 4234-4256 ◽  
Author(s):  
Renan Chiste ◽  
Marisa Freitas ◽  
Adriana Mercadante ◽  
Eduarda Fernandes

2014 ◽  
Vol 12 (3) ◽  
pp. 377-385 ◽  
Author(s):  
Vesna Šaponjac ◽  
Dragana Četojević-Simin ◽  
Gordana Ćetković ◽  
Jasna Čanadanović-Brunet ◽  
Sonja Djilas ◽  
...  

AbstractGround spice paprika was extracted with hexane, by conventional Soxhlet procedure (SX oleoresin), and with supercritical carbon dioxide at three different pressures — 20, 30 and 40 MPa (SF20, SF30 and SF40 oleoresins). The effect of extraction method and conditions on the colour intesity of paprika oleoresins, content of α-tocopherol, as well as antioxidant and antiproliferative activity was examined. Hexane showed highest selectivity for paprika pigments (886.02 ASTA), while α-tocopherol showed highest solubility (3846.9 mg kg−1) in supercritical carbon dioxide at 20 MPa. All paprika oleoresins exhibited good superoxide anion radical scavenging activity SF30 being the best superoxide anion radical scavenger. Cell growth activity was evaluated in vitro in human cell lines:cervix epitheloid carcinoma (HeLa), breast adenocarcinoma (MCF7) and colon adenocarcinoma (HT-29). The highest antiproliferative activity was exhibited by SX in MCF7 cell line (IC50=14.28 mg mL−1). Extract SF40 produced significant and selective antiproliferative action towards HeLa cell line. These results suggest that paprika oleoresins, due to high antiradical and tumor cell-inhibiting activity, can be regarded as functional food ingredients.


Author(s):  
Lun-Yi Zang ◽  
Greg Cosma ◽  
Henry Gardner ◽  
Kenneth Starks ◽  
Xianglin Shi ◽  
...  

Marine Drugs ◽  
2018 ◽  
Vol 16 (10) ◽  
pp. 394 ◽  
Author(s):  
Long-Yan Li ◽  
Yu-Qin Zhao ◽  
Yu He ◽  
Chang-Feng Chi ◽  
Bin Wang

In this report, acid-soluble collagen (ASC-MC) and pepsin-soluble collagen (PSC-MC) were extracted from the scales of miiuy croaker (Miichthys miiuy) with yields of 0.64 ± 0.07% and 3.87 ± 0.15% of dry weight basis, respectively. ASC-MC and PSC-MC had glycine as the major amino acid with the contents of 341.8 ± 4.2 and 344.5 ± 3.2 residues/1000 residues, respectively. ASC-MC and PSC-MC had lower denaturation temperatures (32.2 °C and 29.0 °C for ASC-MC and PSC-MC, respectively) compared to mammalian collagen due to their low imino acid content (197.6 and 195.2 residues/1000 residues for ASC-MC and PSC-MC, respectively). ASC-MC and PSC-MC were mainly composed of type I collagen on the literatures and results of amino acid composition, SDS-PAGE pattern, ultraviolet (UV) and Fourier-transform infrared spectroscopy (FTIR) spectra. The maximum solubility of ASC-MC and PSC-MC was appeared at pH 1–3 and a sharp decrease in solubility was observed when the NaCl concentration was above 2%. Zeta potential studies indicated that ASC-MC and PSC-MC exhibited a net zero charge at pH 6.66 and 6.81, respectively. Furthermore, the scavenging capabilities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical, superoxide anion radical and 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical of ASC-MC and PSC-MC were positively correlated with their tested concentration ranged from 0 to 5 mg/mL and PSC-MC showed significantly higher activity than that of ASC-MC at most tested concentrations (p < 0.05). In addition, the scavenging capability of PSC-MC on hydroxyl radical and superoxide anion radical was higher than those of DPPH radical and ABTS radical, which suggested that ASC-SC and PSC-SC might be served as hydroxyl radical and superoxide anion radical scavenger in cosmeceutical products for protecting skins from photoaging and ultraviolet damage.


PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3050 ◽  
Author(s):  
Ankush Prasad ◽  
Aditya Kumar ◽  
Ryo Matsuoka ◽  
Akemi Takahashi ◽  
Ryo Fujii ◽  
...  

Background The growth and development of plants is deleteriously affected by various biotic and abiotic stress factors. Wounding in plants is caused by exposure to environmental stress, mechanical stress, and via herbivory. Typically, oxidative burst in response to wounding is associated with the formation of reactive oxygen species, such as the superoxide anion radical (O2•−), hydrogen peroxide (H2O2) and singlet oxygen; however, few experimental studies have provided direct evidence of their detection in plants. Detection of O2•− formation in plant tissues have been performed using various techniques including electron paramagnetic resonance spin-trap spectroscopy, epinephrine-adrenochrome acceptor methods, staining with dyes such as tetrazolium dye and nitro blue tetrazolium (NBT); however, kinetic measurements have not been performed. In the current study, we provide evidence of O2•− generation and its kinetics in the leaves of spinach (Spinacia oleracea) subjected to wounding. Methods Real-time monitoring of O2•− generation was performed using catalytic amperometry. Changes in oxidation current for O2•− was monitored using polymeric iron-porphyrin-based modified carbon electrodes (φ = 1 mm) as working electrode with Ag/AgCl as the reference electrode. Result The results obtained show continuous generation of O2•− for minutes after wounding, followed by a decline. The exogenous addition of superoxide dismutase, which is known to dismutate O2•− to H2O2, significantly suppressed the oxidation current. Conclusion Catalytic amperometric measurements were performed using polymeric iron-porphyrin based modified carbon electrode. We claim it to be a useful tool and a direct method for real-time monitoring and precise detection of O2•− in biological samples, with the potential for wide application in plant research for specific and sensitive detection of O2•−.


2013 ◽  
Vol 807-809 ◽  
pp. 1984-1987 ◽  
Author(s):  
Qin Li ◽  
Chi Zhang

In this article,Cordyceps militaris,that from Hubei and Yunnan,were used as the research objects, to extract seleno-polysaccharide ,then the antioxidant activity of seleno-polysaccharide were determined by reducing power,the scavenging capacity on superoxide anion radical and hydroxyl radical.The results indicated that,the content of selenium in Hubei and Yunnan Cordyceps militaris samples were 210.0μg/g and 57.5μg/g,respectively;the share of Se-polysaccharide in total selenium were 11.086% and 26.713%,respectively,lower degree to turn the selenium organic in Hubei Cordyceps militaris.The reducing power of seleno-polysaccharide from Hubei and Yunnan Cordyceps militaris were 0.0253,0.0297,respectively;two kinds of Cordyceps militaris have certain ability to remove superoxide anion radical and different between them,the seleno-polysaccharide of Yunnan Cordyceps militaris has a better effect to remove superoxide anion radical under different sample amount.About hydroxyl radical,there were no ability to remove it.


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