scholarly journals Detección de anticuerpos séricos en toros inmunizados contra campylobacteriosis

2010 ◽  
Vol 21 (1) ◽  
pp. 8
Author(s):  
C. M. Campero ◽  
E. Morrell ◽  
C. Morsella ◽  
F. Paolicchi ◽  
D. Cano ◽  
...  
Keyword(s):  
Buenos Aires ◽  
Tritrichomonas Foetus ◽  
Campylobacter Fetus ◽  
Fetus Subsp ◽  
Los Grupos

<p>En el presente ensayo se evaluó la repuesta sérica vacunando 169 toros jóvenes para carne, vírgenes de 12–24 meses de edad, inmunizados con vacunas a célula entera de Campylobacter fetus. El trabajo se realizó en cuatro establecimientos libres de campylobacteriosis de la provincia de Buenos Aires (Argentina). Se utilizaron tres vacunas comerciales nacionales, conteniendo cepas inactivadas de C. fetus y sus subspecies: vacunas A (n= 84), B (n=19) y C (n=58) (suspensión de C. fetus subsp. fetus, intermedius y venerealis, inactivadas con formol). Las vacunas A y B poseían como vehículo hidróxido de aluminio, la vacuna C presentaba adyuvante oleoso. También se utilizó una vacuna experimental (D) (n=8) dual, oleosa, elaborada por el INTA Balcarce, conteniendo antígenos inactivados de C. fetus subsp. fetus, C. fetus subsp. venerealis y Tritrichomonas foetus. Todas las vacunas se aplicaron por vía subcutánea, dos dosis con intervalo de 21 días. Los animales se sangraron a los días 0, 21, 42, 93 y 123 post primera dosis vacunal (DPV). Los sueros fueron procesados por ELISA indirecto. Los valores de absorbancia expresados en densidad óptica fueron transformados a valores ELISA (VE). La vacuna experimental dual D demostró un incremento importante en los VE de los anticuerpos séricos con respecto las vacunas A, B y C (p&lt; 0,05). Las vacunas B y C tuvieron mejor respuesta a los 21 y 42 días DPV respecto a la vacuna A (p&lt; 0,05). A los 93 DPV, los animales del grupo C tuvieron un débil incremento de VE en comparación con aquellos animales de los grupos A y B (p&lt; 0,05). El desempeño general tendió a ser mayor en los animales inmunizados con la vacuna experimental que aquellos del grupo de vacunas comerciales. Se observaron VE muy bajos en general en las vacunas comerciales utilizadas. El método ELISA fue adecuado para la evaluación de la respuesta inmune sistémica en los toros vacunados. </p>

scholarly journals Prevalence of Tritrichomonas foetus and Campylobacter fetus subsp. venerealis among bulls slaughtered in the state of Minas Gerais, Brazil

2018 ◽  
Vol 39 (5) ◽  
pp. 2039
Author(s):  
Michele Placedino Andrade Botelho ◽  
Christian Hirsch ◽  
Andrey Pereira Lage ◽  
Christiane Maria Barcellos Magalhães da Rocha ◽  
Elaine Maria Seles Dorneles ◽  
...  
Keyword(s):  
Minas Gerais ◽  
Tritrichomonas Foetus ◽  
Sexually Transmitted ◽  
Molecular Tests ◽  
Campylobacter Fetus ◽  
Causative Agents ◽  
Fetus Subsp ◽  
Natural Mating ◽  
Cattle Herds ◽  
Bovine Genital Campylobacteriosis

Bovine genital campylobacteriosis (BGC) and bovine genital trichomonosis (BGT) are globally widespread, predominantly sexually transmitted diseases that mainly affect herds in which natural mating takes place. Bulls serve as good epidemiological indicators for studying the causative agents of these diseases in cattle herds. The objective of the present work was to determine the prevalence of BGC and BGT among bulls sent to slaughterhouses in southern Minas Gerais, Brazil. Samples of preputial smegma from 200 sexually mature, non-castrated bulls of several ancestries were collected in 2013 from four regional slaughterhouses. The polymerase chain reaction (PCR) was employed to detect Tritrichomonas foetus, Campylobacter fetus subsp. fetus, and C. fetus subsp. venerealis. Isolation also was used aiming to diagnosis of BGT. All smegma samples were negative in culture for T. foetus. Molecular tests revealed 8% (16/200) of animals to be positive for T. foetus, 17.5% (35/200) for Campylobacter. fetus subsp. fetus, and 13.5% (27/200) for C. fetus subsp. venerealis. The results of the present study indicate that T. foetus, C. fetus subsp. venerealis, and C. fetus subsp. fetus are present among bulls slaughtered in southern Minas Gerais, and that BGC and BGT occur in this region. These diseases therefore need to be considered during differential diagnosis of reproductive diseases affecting cattle herds in which natural mating is employed.

scholarly journals Prevalence of genital campylobacteriosis and trichomonosis in crossbred breeding bulls kept on zero-grazed smallholder dairy farms in the Tanga region of Tanzania

2005 ◽  
Vol 76 (4) ◽  
Author(s):  
E.S. Swai ◽  
J. Hulsebosch ◽  
W. Van der Heijden
Keyword(s):  
Dairy Farms ◽  
Significant Problem ◽  
Tritrichomonas Foetus ◽  
Campylobacter Fetus ◽  
Fetus Subsp ◽  
Smallholder Dairy ◽  
Smallholder Dairy Farms

A survey to demonstrate the presence or absence of genital campylobacteriosis and trichomonosis in cross-bred breeding bulls kept under smallholding dairy farms in the Tanga region of Tanzania was carried out during the period of January-June 1996. Sheath washings, swabs and preputial scrapings were collected from 58 randomly selected bulls. Campylobacter fetus subsp. venerealis was demonstrated in 3/58 (5.1 %) and Tritrichomonas foetus in 0/58 (0 %) of all bulls tested. Bull-level variables of level of taurine genes (62.5 % taurine genes, F2; 75 % taurine genes, F3) and age were not significantly associated with campylobacteriosis (P > 0.05). The result of the study identifies Campylobacter fetus subsp. venerelias as the agent of enzootic infertility in smallholder herds and suggests that may be a significant problem.

scholarly journals Ocorrência da infecção por Campylobacter fetus subsp. venerealis e Tritrichomonas foetus em búfalos no estado de Pernambuco, Brasil

2018 ◽  
Vol 70 (2) ◽  
pp. 457-462
Author(s):  
J.M. Borges ◽  
L.B.F. Soares ◽  
B.P. Silva ◽  
A.A. Macedo ◽  
J.M.B. Oliveira ◽  
...  
Keyword(s):  
Tritrichomonas Foetus ◽  
Campylobacter Fetus ◽  
De Se ◽  
Fetus Subsp

RESUMO Objetivou-se com estudo determinar a ocorrência da infecção por Campylobacter fetus subsp. venerealis e Tritrichomonas foetus em búfalos no estado de Pernambuco, Brasil. Foram coletadas 133 amostras biológicas (muco cervicovaginal e raspado prepucial) de animais, procedentes de oito propriedades, de diferentes regiões do estado. O material biológico coletado foi transferido para solução salina tamponada (PBS) e, posteriormente, inoculado em meios de transporte específicos, Lander para diagnóstico de C. fetus subsp. venerealis e Diamond para T. foetus. Para o diagnóstico das infecções por Campylobacter fetus subsp. venerealis e Tritrichomonas foetus, as amostras foram submetidas à reação em cadeia da polimerase (PCR) e cultivadas em meio ágar Columbia acrescido de antibiótico e Diamond, respectivamente. Para pesquisa de C. fetus subsp. venerealis, observou-se uma ocorrência de 1,8% (2/113) de animais positivos no exame microbiológico com confirmação pela PCR. Em relação à procedência, observou-se que 100% das amostras positivas pertenciam a dois machos do mesmo rebanho. Nenhum animal foi positivo na pesquisa de T. foetus. Este é o primeiro registro da infecção por C. fetus subsp. venerealis em búfalos no Brasil. Apesar da baixa ocorrência, recomenda-se adoção de medidas de controle, com o intuito de se evitar a disseminação do agente para outros rebanhos.

scholarly journals Occurrence of Campylobacter fetus subsp. venerealis and Tritrichomonas foetus dna in bulls from Alagoas State, Brazil

2018 ◽  
Vol 39 (6) ◽  
pp. 2477
Author(s):  
Glaucia Grazielle Nascimento ◽  
Pollyanne Raysa Fernandes de Oliveira ◽  
Edson Moura da Silva ◽  
Rinaldo Aparecido Mota ◽  
José Wilton Pinheiro Junior
Keyword(s):  
Polymerase Chain Reaction ◽  
Tritrichomonas Foetus ◽  
Chain Reaction ◽  
Campylobacter Fetus ◽  
Fetus Subsp ◽  
Laboratory Examinations ◽  
Polymerase Chain ◽  
And Control ◽  
Monitor And Control

The aim of the present study is to diagnose the occurrence of infections caused by Campylobacter fetus subsp. venerealis and Tritrichomonas foetus in reproducer bulls from Alagoas State breeders, Brazil. The total of 162 preputial smegma samples were collected from nelore bulls from ten rural properties in the East, Agreste and Sertão mesoregions. The samples were subjected to the Polymerase Chain Reaction (PCR) technique in order to assess C. fetus subsp. venerealis and Tritrichomonas foetus DNA and cultivated in Modified Diamond Medium (DMM) for Tritrichomonas foetus isolation. Four point nine percent (4.9% - 8/162) of the evaluated bulls were infected with C. fetus subsp. venerealis and 3.0% (5/162) of the sample were infected with T. foetus, which was not isolated in any of the assessed animals. Based on our results, there was C. fetus subsp. venerealis and T. foetus DNA in bulls from Alagoas State, Brazil. Accordingly, it is necessary performing laboratory examinations in animals living in properties breeding animals for reproduction purpose in order to monitor and control such infections.

IMMUNISATION AGAINST BOVINE VIBRIOSIS DUE TO CAMPYLOBACTER FETUS SUBSP. FETUS BIOTYPE INTERMEDIUS

1976 ◽  
Vol 52 (8) ◽  
pp. 362-365 ◽  
Author(s):  
B. L. Clark ◽  
J. H. Dufty ◽  
Mary J. Monsbourgh ◽  
I. M. Parsonson
Keyword(s):  
Campylobacter Fetus ◽  
Fetus Subsp

scholarly journals Colonización del espacio, la palabra y el cuerpo en el Chaco argentino

2003 ◽  
Vol 9 (19) ◽  
pp. 137-152 ◽  
Author(s):  
Pablo G. Wright
Keyword(s):  
Buenos Aires ◽  
Los Grupos

Este trabajo explora los efectos que tuvo la conquista y colonización del Chaco argentino en relación al espacio, la palabra y el cuerpo de los aborígenes chaqueños. Este proceso, que adquirió una dinámica rápida y violenta desde las expediciones militares enviadas por el gobierno de Buenos Aires entre1884 y 1911, produjo transformaciones profundas en el modo de vida de los grupos chaqueños (especialmente los mocovíes, tobas y pilagás del tronco lingüístico guaykurú, y de los wichí, del mataco-maká). Me interesa enfocar la mirada en las consecuencias de la política oficial de "pacificación" y "civilización" de los indígenas a través del sistema de "reducciones" estatales y religiosas, y otros modos modos de acción asociados a éstos, y el impacto que tuvieron en la construcción de la legalidad, la oralidad y la escritura, y el destino de los cuerpos disciplinados por las rutinas del "trabajo" y el manejo del espacio propios de la concepción capitalista occidental del trabajo, que se intentaban implantar en la región.

scholarly journals Influence of Campylobacter fetus subsp. fetus on ram sperm cell quality

2008 ◽  
Vol 57 (11) ◽  
pp. 1405-1410 ◽  
Author(s):  
Tidhar Zan Bar ◽  
Ronen Yehuda ◽  
Tomer Hacham ◽  
Sigal Krupnik ◽  
Benjamin Bartoov
Keyword(s):  
Sperm Cell ◽  
Sperm Cells ◽  
Male Factor ◽  
Motility Index ◽  
Fas Receptor ◽  
Campylobacter Fetus ◽  
Fetus Subsp ◽  
Ram Sperm ◽  
Female Sheep

Campylobacter fetus subsp. fetus infection can occur in female sheep, causing infertility or abortion. Despite extensive research on the effect of these bacteria on female fertility, little research has been done on the influence of C. fetus subsp. fetus on the male factor. Our objective was to examine the influence of C. fetus subsp. fetus on ram sperm. Motility index, percentage of live spermatozoa, mean αt value (an indication of the chromatin stability of the sperm cell) and percentage of sperm cells expressing the FAS receptor were measured in sperm incubated in the presence or absence of C. fetus subsp. fetus. The motility index and viability of sperm incubated with the bacteria were lower than those of untreated sperm samples after 5 h. In bacteria-incubated sperm cells, the percentage expressing FAS receptor was already significantly elevated at 15 min. Bacteria-incubated sperm showed a greater prevalence of morphological damage. The bacteria were attached to tail and acrosome regions, and the sperm damage was concentrated in both the motility and chromatin regions. Bacteria-infected sperm cells showed a decrease in motility, increase in early acrosome reaction and chromatin damage. Similar effects were induced by incubation of the sperm with supernatants from C. fetus subsp. fetus cultures. Thus this study demonstrates that C. fetus subsp. fetus has a detrimental effect on the quality of ram sperm.

scholarly journals Selection and characterization of peptides mimetic to Campylobacter fetus subsp. venerealis using phage display

2021 ◽  
Vol 51 (8) ◽  
Author(s):  
Eduardo Lara Ribeiro ◽  
Patrícia Tiemi Fujimura ◽  
Carlos Ueira-Vieira ◽  
Luiz Ricardo Goulart ◽  
Telma Maria Alves ◽  
...  
Keyword(s):  
Phage Display ◽  
Phage Library ◽  
Hyperimmune Serum ◽  
Subclinical Disease ◽  
Campylobacter Fetus ◽  
Fetus Subsp ◽  
Cattle Herds ◽  
Cervicovaginal Mucus ◽  
Bovine Genital Campylobacteriosis

ABSTRACT: Bovine genital campylobacteriosis (BGC) is a venereal and subclinical disease that affects the fertility of cattle herds, and it is caused by Campylobacter fetus subsp. venerealis . This study selected peptides mimetic to the BGC-causing agent from a phage library. Phage display is a technique that applies bacteriophage libraries that reveal peptides fused to the viral capsid in biological selections against target proteins. Biopannings were performed for biological selection in the phage library using rabbit hyperimmune serum and C. fetus subsp. venerealis protein extract. Five selected heptapeptides were considered mimetic to Cfv-NCTC 10354 based on the results of bioinformatics analysis and assays with hyperimmune serum and cervicovaginal mucus obtained from heifers. ALASLPL and LSYLFPP were the most reactive peptides and considered promising as possible mimetic immunogens for C. fetus subsp. venerealis.

scholarly journals La terminología de parentesco de los indios borucas

2006 ◽  
Vol 3 (5) ◽  
pp. 31
Author(s):  
Adolfo Constenla Umaña
Keyword(s):  
Buenos Aires ◽  
Los Grupos

Los borucas, uno de los grupos indígenas costarricenses que conservan su idioma, tienen sus poblados en la reserva Térraba-Boruca (cantón de Buenos Aires, provincia de Puntarenas) en la zona sur del litoral del país.Su número es aproximadamente de 800 personas, desconociéndose cuántas de ellas hablan todavía su lengua, que, como las otras del área (bribrí, cabécar y térraba) pertenecen al grupo talamanqueño de la rama occidental de la familia chibcha.

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