scholarly journals Occurrence of Campylobacter fetus subsp. venerealis and Tritrichomonas foetus dna in bulls from Alagoas State, Brazil

2018 ◽  
Vol 39 (6) ◽  
pp. 2477
Author(s):  
Glaucia Grazielle Nascimento ◽  
Pollyanne Raysa Fernandes de Oliveira ◽  
Edson Moura da Silva ◽  
Rinaldo Aparecido Mota ◽  
José Wilton Pinheiro Junior
Keyword(s):  
Polymerase Chain Reaction ◽  
Tritrichomonas Foetus ◽  
Chain Reaction ◽  
Campylobacter Fetus ◽  
Fetus Subsp ◽  
Laboratory Examinations ◽  
Polymerase Chain ◽  
And Control ◽  
Monitor And Control

The aim of the present study is to diagnose the occurrence of infections caused by Campylobacter fetus subsp. venerealis and Tritrichomonas foetus in reproducer bulls from Alagoas State breeders, Brazil. The total of 162 preputial smegma samples were collected from nelore bulls from ten rural properties in the East, Agreste and Sertão mesoregions. The samples were subjected to the Polymerase Chain Reaction (PCR) technique in order to assess C. fetus subsp. venerealis and Tritrichomonas foetus DNA and cultivated in Modified Diamond Medium (DMM) for Tritrichomonas foetus isolation. Four point nine percent (4.9% - 8/162) of the evaluated bulls were infected with C. fetus subsp. venerealis and 3.0% (5/162) of the sample were infected with T. foetus, which was not isolated in any of the assessed animals. Based on our results, there was C. fetus subsp. venerealis and T. foetus DNA in bulls from Alagoas State, Brazil. Accordingly, it is necessary performing laboratory examinations in animals living in properties breeding animals for reproduction purpose in order to monitor and control such infections.

scholarly journals How prevalent is Plasmodium malariae in Rondônia, Western Brazilian Amazon?

2000 ◽  
Vol 33 (5) ◽  
pp. 489-492 ◽  
Author(s):  
Marisa Torres Vidal Cavasini ◽  
Weber Luidi Ribeiro ◽  
Fumihiko Kawamoto ◽  
Marcelo Urbano Ferreira
Keyword(s):  
Polymerase Chain Reaction ◽  
Plasmodium Species ◽  
Brazilian Amazon ◽  
Plasmodium Malariae ◽  
Mixed Species ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain ◽  
Potential Impact ◽  
And Control

We have compared results of Plasmodium species identification obtained with conventional on-site microscopy of Giemsa-stained thick smears (GTS) and a semi-nested polymerase chain reaction (PCR) in 96 malaria patients from Rondônia, Western Brazilian Amazon. Mixed-species infections were detected by PCR in 30% patients, but no such case had been found on GTS. Moreover, P. malariae infections were detected in 9 of 96 patients (10%) by PCR, but were not identified by local microscopists. The potential impact of species misidentification on malaria treatment and control is discussed.

scholarly journals Polymerase chain reaction for the diagnosis of bovine genital campylobacteriosis

2010 ◽  
Vol 30 (12) ◽  
pp. 1031-1035 ◽  
Author(s):  
Ana C.M. Groff ◽  
Jackeline K. Kirinus ◽  
Mariana Sá e Silva ◽  
Gustavo Machado ◽  
Mateus M. Costa ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Clinical Samples ◽  
Hexadecyltrimethylammonium Bromide ◽  
Bacterial Isolation ◽  
Chain Reaction ◽  
Guanidine Isothiocyanate ◽  
Campylobacter Fetus ◽  
Polymerase Chain ◽  
Bovine Genital Campylobacteriosis

Bovine genital campylobacteriosis is a common venereal disease of cattle; the prevalence of this disease can be underestimated mostly because of the nature of the etiological agent, the microaerobic Campylobacter fetus subspecies venerealis. The purpose of the current study was to evaluate the utilization of polymerase chain reaction (PCR) in the diagnosis of genital campylobacteriosis in samples obtained from bull prepuce aspirate, cow cervical mucus, and abomasum contents of aborted fetuses, collected into enrichment medium. Five different DNA extraction protocols were tested: thermal extraction, lysis with proteinase K, lysis with guanidine isothiocyanate, lysis with DNAzol, and lysis with hexadecyltrimethylammonium bromide (CTAB). The specificity, sensitivity, and technical application of the PCR assay were also evaluated with clinical samples and compared to bacterial isolation by standard culture. DNA extraction by the CTAB protocol provided better results in PCR, and it was able to detect 63 colony-forming units per ml of C. fetus. Out of 277 clinical samples tested, 68 (24%) were positive for Campylobacter fetus using PCR, while only 8 (2.8%) of the samples were positive by bacterial isolation in solid medium, proving the superiority of the PCR technique when compared to the standard isolation method, and providing evidence for its usefulness as a better screening test in cattle for the diagnosis of bovine genital campylobacteriosis.

Prevalence of Tritrichomonas foetus infection in cats with diarrhoea in the UK

2007 ◽  
Vol 9 (3) ◽  
pp. 214-218 ◽  
Author(s):  
Danièlle A. Gunn-Moore ◽  
Theresa M. McCann ◽  
Nicki Reed ◽  
Kerry E. Simpson ◽  
Bryn Tennant
Keyword(s):  
Polymerase Chain Reaction ◽  
Tritrichomonas Foetus ◽  
Chain Reaction ◽  
Faecal Samples ◽  
The Usa ◽  
Polymerase Chain ◽  
The Uk

Faecal samples from 111 cats with diarrhoea that were living in the UK were submitted for the assessment of Tritrichomonas foetus infection by polymerase chain reaction (PCR). Sixteen (14.4%) samples were found to be positive. In agreement with studies from the USA, infected cats were predominantly of a year of age or less and of a pedigree breed, with Siamese and Bengal cats specifically over-represented in this population.

scholarly journals Neonatal SARS-CoV-2 Infection and Congenital Myocarditis: A Case Report and Literature Review

2020 ◽  
Vol 8 (3) ◽  
Author(s):  
Reza Amiraskari ◽  
Elaheh Sayarifard ◽  
Hamed Kharrazi ◽  
Neda Naserfar ◽  
Azadeh Sayarifard
Keyword(s):  
Polymerase Chain Reaction ◽  
Literature Review ◽  
Vertical Transmission ◽  
Prevention And Control ◽  
Cardiac Injury ◽  
Insufficient Evidence ◽  
Chain Reaction ◽  
Case Presentation ◽  
Polymerase Chain ◽  
And Control

Introduction: With the outbreak of coronavirus 2019 (SARS-COV-2), the prevention and control of SARS-COV-2 infection in pregnant women and the potential risk of vertical transmission has become a major concern. Case Presentation: We report the case of a newborn in Iran with the manifestations of myocarditis at birth. The diagnosis of SARS-COV-2 infection was confirmed for the mother and the neonate by real-time reverse transcription polymerase chain reaction (rRT-PCR) using a pharyngeal specimen. Conclusions: Based on our literature review, there is still insufficient evidence to determine the effect of SARS-COV-2 infection on the fetus. Given the possibility of cardiac injury in SARS-COV-2 disease and manifestation of congenital myocarditis in our case, maternal vertical transmission of SARS-COV-2 could be considered.

Detection of α-Thalassemia of Southeast Asian (--SEA) Deletion by Novel Multiplex PCR

2020 ◽  
Vol 103 (8) ◽  
pp. 741-747
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Pcr ◽  
Control Strategy ◽  
Predictive Value ◽  
Prevention And Control ◽  
Globin Gene ◽  
Southeast Asian ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
And Control

Background: Southeast Asian (--SEA) deletion of α-thalassemia is the most common α-thalassemia-1 mutation in Thailand and neighborhood countries. The absence of α-globin gene production in a homozygous fetus is the most serious and leads to death in utero or soon after birth and life-threatening maternal complications. Therefore, sensitive and accurate assays for detecting α-thalassemia--SEA deletion are crucial for thalassemia diagnosis. Materials and Methods: The present study evaluated multiplex polymerase chain reaction (PCR)--SEA, comparing with the routinely performed gap-PCR for α-thalassemia diagnosis in prenatal thalassemia prevention and control strategy commonly used in Thailand. Four primers were employed to detect the deleted region of α-globin gene family. This, thus, provides high accuracy in discriminating heterozygous and homozygous --SEA deletion. Results: Multiplex PCR--SEA assay showed the results with 100% sensitivity, specificity, positive predictive value, negative predictive value, and efficiency in comparison to the routinely performed gap-PCR used in prenatal thalassemia prevention and control strategy. In addition, the multiplex PCR--SEA assay displayed lower detection limit for heterozygous detection. Conclusion: The novel multiplex PCR--SEA in the present study was proofed to be a good alternative for thalassemia diagnosis in thalassemia prevention and control strategy. The advantage of the assay is the capability to identify three wild types and one deleted fragment comparing with one wild type and one deleted fragment in the routinely performed gap-PCR. This is very useful, especially in the genes where polymorphism is common. Therefore, the multiplex PCR--SEA provides a better protocol for α-thalassemia--SEA diagnosis. Keywords: α-thalassemia, Hemoglobin Bart’s hydrop fetalis, Multiplex PCR, Polymerase chain reaction, Prenatal diagnosis, --SEA deletion

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