Bromothymol Blue TS

2021 ◽  
Keyword(s):  
Bromothymol Blue

scholarly journals CO2 Convective Dissolution in Oil-Saturated Unconsolidated Porous Media at Reservoir Conditions

Energies ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 233
Author(s):  
Widuramina Amarasinghe ◽  
Ingebret Fjelde ◽  
Nils Giske ◽  
Ying Guo
Keyword(s):  
Porous Media ◽  
Crude Oil ◽  
Water Solution ◽  
Co2 Storage ◽  
Bromothymol Blue ◽  
Power Relationship ◽  
Dimensionless Time ◽  
3 Dimensional ◽  
Reservoir Conditions ◽  
Unconsolidated Porous Media

During CO2 storage, CO2 plume mixes with the water and oil present at the reservoir, initiated by diffusion followed by a density gradient that leads to a convective flow. Studies are available where CO2 convective mixing have been studied in water phase but limited in oil phase. This study was conducted to reach this gap, and experiments were conducted in a vertically packed 3-dimensional column with oil-saturated unconsolidated porous media at 100 bar and 50 °C (representative of reservoir pressure and temperature conditions). N-Decane and crude oil were used as oils, and glass beads as porous media. A bromothymol blue water solution-filled sapphire cell connected at the bottom of the column was used to monitor the CO2 breakthrough. With the increase of the Rayleigh number, the CO2 transport rate in n-decane was found to increase as a function of a second order polynomial. Ra number vs. dimensionless time τ had a power relationship in the form of Ra = c×τ−n. The overall pressure decay was faster in n-decane compared to crude oil for similar permeability (4 D), and the crude oil had a breakthrough time three times slower than in n-decane. The results were compared with similar experiments that have been carried out using water.

Acid–base sensor based on sol–gel encapsulation of bromothymol blue in silica: application for milk spoilage detection

2021 ◽  
Author(s):  
Mariele K. Stocker ◽  
Murilo L. Sanson ◽  
Arthur A. Bernardes ◽  
Antonio Marchi Netto ◽  
Rodrigo Brambilla
Keyword(s):  
Sol Gel ◽  
Bromothymol Blue ◽  
Acid Base

scholarly journals Conjugation of Peroxidase from Brassica oleracea gongylodes for Use as a Label- Prospect of a Novel Enzyme Tag for Immunoassay Systems

2017 ◽  
Vol 5 (1) ◽  
pp. 59-65 ◽  
Author(s):  
Premalatha Shetty ◽  
Avila D’Souza ◽  
Geethu CP
Keyword(s):  
Brassica Oleracea ◽  
Temperature Stability ◽  
Egg Yolk ◽  
Bromothymol Blue ◽  
Bromocresol Purple ◽  
Significant Extent ◽  
Periodate Treatment ◽  
Ammonium Sulfate Saturation ◽  
Tissue Antigens

Peroxidase tagged proteins are being used successfully as immune-histological probes for the demonstration of tissue antigens, and in enzyme amplified immunoassay systems for the quantitative determination of soluble and insoluble antigens. The glycoprotein nature of peroxidases can be exploited for conjugation to proteins of interest. Peroxidase extracted from the bulbs of Brassica oleracea gongylodes was salted out at 40-80% ammonium sulfate saturation and activated by treatment with 1-Fluoro-2,4-dinitro benzene (FDNB) and periodate. Treatment with 0.08% FDNB and 12.5mM periodate was optimized for activation of the enzyme. The treated enzyme was found to conjugate successfully to immunoglobulin fractions harvested from egg yolk (IgY), human plasma and goat serum. Enzyme conjugated to IgY fraction showed improvement in its pH stability and temperature stability. The affinity of the enzyme for its substrate phenol did not alter to a significant extent upon activation and conjugation. The conjugates exhibited high affinity towards phenol, bromocresol purple and bromothymol blue in comparison to HRP conjugates prepared using the same protocol. Int. J. Appl. Sci. Biotechnol. Vol 5(1): 59-65

An Intelligent Film Based on Cassia Gum Containing Bromothymol Blue-Anchored Cellulose Fibers for Real-Time Detection of Meat Freshness

2019 ◽  
Vol 67 (7) ◽  
pp. 2066-2074 ◽  
Author(s):  
Lele Cao ◽  
Guohou Sun ◽  
Cijian Zhang ◽  
Wenbo Liu ◽  
Jian Li ◽  
...  
Keyword(s):  
Real Time ◽  
Cellulose Fibers ◽  
Bromothymol Blue ◽  
Real Time Detection

scholarly journals Extractive Spectrophotometric Methods for Determination of Zolmitriptan in Tablets

2007 ◽  
Vol 90 (5) ◽  
pp. 1237-1241 ◽  
Author(s):  
Zeynep Aydogmus ◽  
Ipek Inanli
Keyword(s):  
High Performance ◽  
Correlation Coefficients ◽  
Bromothymol Blue ◽  
Chromatography Method ◽  
Statistical Comparison ◽  
Spectrophotometric Methods ◽  
Accuracy And Precision ◽  
Liquid Chromatography Method ◽  
Comparison Of The Results

Abstract Two simple and sensitive extractive spectrophotometric methods have been developed for determination of zolmitriptan (ZTP) in tablets. These methods are based on the formation of yellow ion-pair complexes between ZTP and tropaeolin OO (TPOO) and bromothymol blue (BTB) in citratephosphate buffer of pH 4.0 and 6.0, respectively. The formed complexes were extracted with dichloromethane and measured at 411.5 and 410 nm for TPOO and BTB, respectively. The best conditions of the reactions were studied and optimized. Beer's law was obeyed in the concentration ranges of 220 and 1.517 g/mL with molar absorptivities of 1.42 104 and 1.60 104 L/mol/cm for the TPOO and BTB methods, respectively. Correlation coefficients were 0.9998 and 0.9999 for TPOO and BTB methods, respectively. Limits of detection of the TPOO and BTB methods were 0.341 and 0.344 g/mL, respectively, and the limits of quantitation were 1.034 and 1.051 g/mL, respectively. Sandell's sensitivity and stability constant were also calculated. The proposed methods have been applied successfully for the analysis of the drug in its dosage forms. No interference was observed from excipients present in tablets. Statistical comparison of the results with those obtained by a high-performance liquid chromatography method showed excellent agreement and indicated no significant differences in accuracy and precision.

scholarly journals Two-layer Electrospun System Enabling Wound Exudate Management and Visual Infection Response

Sensors ◽  
2019 ◽  
Vol 19 (5) ◽  
pp. 991 ◽  
Author(s):  
Mohamed Bazbouz ◽  
Giuseppe Tronci
Keyword(s):  
Chronic Wound ◽  
Colour Change ◽  
Single Layer ◽  
Building Blocks ◽  
High Water ◽  
Bromothymol Blue ◽  
Wound Management ◽  
Wound Exudate ◽  
Management Capability ◽  
Chronic Wound Management

The spread of antimicrobial resistance calls for chronic wound management devices that can engage with the wound exudate and signal infection by prompt visual effects. Here, the manufacture of a two-layer fibrous device with independently-controlled exudate management capability and visual infection responsivity was investigated by sequential free surface electrospinning of poly(methyl methacrylate-co-methacrylic acid) (PMMA-co-MAA) and poly(acrylic acid) (PAA). By selecting wound pH as infection indicator, PMMA-co-MAA fibres were encapsulated with halochromic bromothymol blue (BTB) to trigger colour changes at infection-induced alkaline pH. Likewise, the exudate management capability was integrated via the synthesis of a thermally-crosslinked network in electrospun PAA layer. PMMA-co-MAA fibres revealed high BTB loading efficiency (>80 wt.%) and demonstrated prompt colour change and selective dye release at infected-like media (pH > 7). The synthesis of the thermally-crosslinked PAA network successfully enabled high water uptake (WU = 1291 ± 48 − 2369 ± 34 wt.%) and swelling index (SI = 272 ± 4 − 285 ± 3 a.%), in contrast to electrospun PAA controls. This dual device functionality was lost when the same building blocks were configured in a single-layer mesh of core-shell fibres, whereby significant BTB release (~70 wt.%) was measured even at acidic pH. This study therefore demonstrates how the fibrous configuration can be conveniently manipulated to trigger structure-induced functionalities critical to chronic wound management and monitoring.

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