scholarly journals Conjugation of Peroxidase from Brassica oleracea gongylodes for Use as a Label- Prospect of a Novel Enzyme Tag for Immunoassay Systems

2017 ◽  
Vol 5 (1) ◽  
pp. 59-65 ◽  
Author(s):  
Premalatha Shetty ◽  
Avila D’Souza ◽  
Geethu CP
Keyword(s):  
Brassica Oleracea ◽  
Temperature Stability ◽  
Egg Yolk ◽  
Bromothymol Blue ◽  
Bromocresol Purple ◽  
Significant Extent ◽  
Periodate Treatment ◽  
Ammonium Sulfate Saturation ◽  
Tissue Antigens

Peroxidase tagged proteins are being used successfully as immune-histological probes for the demonstration of tissue antigens, and in enzyme amplified immunoassay systems for the quantitative determination of soluble and insoluble antigens. The glycoprotein nature of peroxidases can be exploited for conjugation to proteins of interest. Peroxidase extracted from the bulbs of Brassica oleracea gongylodes was salted out at 40-80% ammonium sulfate saturation and activated by treatment with 1-Fluoro-2,4-dinitro benzene (FDNB) and periodate. Treatment with 0.08% FDNB and 12.5mM periodate was optimized for activation of the enzyme. The treated enzyme was found to conjugate successfully to immunoglobulin fractions harvested from egg yolk (IgY), human plasma and goat serum. Enzyme conjugated to IgY fraction showed improvement in its pH stability and temperature stability. The affinity of the enzyme for its substrate phenol did not alter to a significant extent upon activation and conjugation. The conjugates exhibited high affinity towards phenol, bromocresol purple and bromothymol blue in comparison to HRP conjugates prepared using the same protocol. Int. J. Appl. Sci. Biotechnol. Vol 5(1): 59-65

scholarly journals Extractive Spectrophotometric Determination of Tenofovir Disoproxil Fumarate Using Acidic Triphenylmethane Dyes

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
K. Susmitha ◽  
M. Thirumalachary ◽  
T. Charan Singh ◽  
G. Venkateshwarlu
Keyword(s):  
Tenofovir Disoproxil Fumarate ◽  
Pharmaceutical Formulations ◽  
Transcriptase Inhibitor ◽  
Bromothymol Blue ◽  
Bromophenol Blue ◽  
Bromocresol Purple ◽  
Triphenylmethane Dyes ◽  
Spectrophotometric Methods ◽  
Nucleotide Reverse Transcriptase Inhibitor

Tenofovir disoproxil fumarate is a nucleotide reverse transcriptase inhibitor that has activity against the hepatitis B and HIV viruses. Three simple and sensitive extractive spectrophotometric methods have been described for the assay of tenofovir disoproxil fumarate either in pure form or in pharmaceutical formulations. The developed methods involve formation of colored chloroform extractable ion-pair complexes of the drugs with triphenylmethane dyes, namely, bromothymol blue (BTB), bromophenol blue (BPB), and bromocresol purple (BCP) in acidic medium. The extracted complexes showed absorbance maxima between 410 and 415 nm. Beer’s law is obeyed in the concentration ranges 1.5–25, 1.0–25, and 1.25–25 μg mL−1 with BTB, BPB, and BCP, respectively. The effectc of concentration of dye, pH, and interference of excipients have been studied and optimized. The limits of detection and quantification have been determined. All three methods are validated as per the guidelines of ICH. The methods have been applied to the determination of drug in commercial tablets and results of analysis were validated statistically through recovery studies.

scholarly journals Spectrophotometric Determination of Gemifloxacin Mesylate, Moxifloxacin Hydrochloride, and Enrofloxacin in Pharmaceutical Formulations Using Acid Dyes

2014 ◽  
Vol 2014 ◽  
pp. 1-16 ◽  
Author(s):  
Ayman A. Gouda ◽  
Alaa S. Amin ◽  
Ragaa El-Sheikh ◽  
Amira G. Yousef
Keyword(s):  
Pharmaceutical Formulations ◽  
Bromothymol Blue ◽  
Bromocresol Green ◽  
Bromophenol Blue ◽  
Bromocresol Purple ◽  
Acid Dyes ◽  
Spectrophotometric Methods ◽  
Significant Difference ◽  
Comparison Of The Results

Simple, rapid, and extractive spectrophotometric methods were developed for the determination of some fluoroquinolones antibiotics: gemifloxacin mesylate (GMF), moxifloxacin hydrochloride (MXF), and enrofloxacin (ENF) in pure forms and pharmaceutical formulations. These methods are based on the formation of ion-pair complexes between the basic drugs and acid dyes, namely, bromocresol green (BCG), bromocresol purple (BCP), bromophenol blue (BPB), bromothymol blue (BTB), and methyl orange (MO) in acidic buffer solutions. The formed complexes were extracted with chloroform and measured at 420, 408, 416, 415, and 422 nm for BCG, BCP, BPB, BTB, and MO, respectively, for GMF; at 410, 415, 416, and 420 nm for BCP, BTB, BPB, and MO, respectively, for MXF; and at 419 and 414 nm for BCG and BTB, respectively, in case of ENF. The analytical parameters and their effects are investigated. Beer’s law was obeyed in the ranges 1.0–30, 1.0–20, and 2.0–24 μg mL−1for GMF, MXF, and ENF, respectively. The proposed methods have been applied successfully for the analysis of the studied drugs in pure forms and pharmaceutical formulations. Statistical comparison of the results with the reference methods showed excellent agreement and indicated no significant difference in accuracy and precision.

Determination of Suitable Kohlrabi (Brassica oleracea var. gongylodes) Cultivars for Pickle Preparation

2010 ◽  
Vol 15 (2) ◽  
pp. 152-158 ◽  
Author(s):  
Myung-Ki Lee ◽  
Hye-Jung Yang ◽  
Su-Kyung Kim ◽  
Su-Hyoung Park ◽  
Sung-Won Moon
Keyword(s):  
Brassica Oleracea

Determination of the residue behavior of isocycloseram in Brassica oleracea and soil using the QuEChERS method coupled with HPLC

2021 ◽  
pp. 130734
Author(s):  
Juan Luo ◽  
Chuanfei Bian ◽  
Lei Rao ◽  
Wenwen Zhou ◽  
Yuqi Li ◽  
...  
Keyword(s):  
Brassica Oleracea ◽  
Quechers Method

scholarly journals Determination of egg yolk content in egg liqueurs

2011 ◽  
Vol 22 (No. 1) ◽  
pp. 9-15 ◽  
Author(s):  
H. Čížková ◽  
M. Voldřich ◽  
V. Prokorátová ◽  
F. Kvasnička
Keyword(s):  
Dry Matter ◽  
Raw Materials ◽  
Egg Yolk ◽  
Local Market ◽  
Chemical Markers ◽  
Regression Analyses ◽  
Food Law ◽  
Minimal Concentration ◽  
Egg Composition

The recent Czech Food law (Decree No. 264/2003, 93/2000 and 57/2003 of the law No. 110/1997 as amended) requires the minimal concentration of egg or egg yolk content in relevant food products (mayonnaises, egg pastas, egg liqueurs), however, the methods for the determination of egg and/or egg yolk content are not sufficiently specified. The presented study deals with the development and evaluation of the analytical methods for the determination of egg yolk content in egg liqueurs. Due to the high variability of the egg composition and a possible effect of processing on the composition of the product, several chemical markers were taken into the consideration: dry matter, phosphorus, fat, cholesterol, fatty acids, and lysozyme concentrations. The egg yolk content was estimated by means of multiple regression analyses of the calibration set (model samples) and the data obtained for raw materials and described in literature. According to the egg yolk content determined, only 6 from 10 analysed samples of egg liqueurs obtained from the local market met the limit of 140 g/l (calculated with the 10% standard deviation error of estimation) required by the recent Czech legislation.  

scholarly journals Spectrophotometric Determination of Doxazosin Mesylate in Tablets by Ion-Pair and Charge-Transfer Complexation Reactions

2009 ◽  
Vol 92 (1) ◽  
pp. 131-137 ◽  
Author(s):  
Zeynep Aydomu ◽  
Asli Barla
Keyword(s):  
Charge Transfer ◽  
Ion Pair ◽  
Bromophenol Blue ◽  
Bromocresol Purple ◽  
Charge Transfer Reaction ◽  
Accuracy And Precision ◽  
Doxazosin Mesylate ◽  
Significant Difference ◽  
Complexation Reactions

Abstract Two accurate, easy spectrophotometric methods for the determination of doxazosin mesylate were described. The first method was based on the formation of ion-pair complexes with the acidic sulfophthalein dyes bromocresol purple (BCP) and bromophenol blue (BPB) in pH 3.3 and 4.5 citratephosphate buffer, respectively. The formed complexes were extracted into dichloromethane, and their absorbance was measured at 403 and 410 nm for BCP and BPB, respectively. The second method was based on the charge transfer reaction of the drug as an n-electron donor with either 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) or 7,7,8,8-tetracyanoquinodimethane (TCNQ) as -acceptors, to give colored radical anions. The absorbances of products were measured at 457 nm in acetonitrile and 838 nm in methanol for DDQ and TCNQ, respectively. Under the optimum reaction conditions, Beer's law was obeyed with a good correlation coefficient (r = 0.99970.9999) in the concentration ranges 3.018.0, 3.020.0, 15.095.0, and 10.0100.0 g/mL for the BCP, BPB, DDQ, and TCNQ methods, respectively. Limits of detection of the BCP, BPB, DDQ, and TCNQ methods were 0.314, 0.408, 1.935, and 1.610 g/mL, respectively. The limits of quantification were 1.045, 1.360, 6.449, and 5.367 g/mL, respectively. The parameters molar absorptivity, precision, accuracy, recovery, robustness, and stability constant were studied. The proposed methods were successfully applied for determination of the drug in tablets with good accuracy and precision. Statistical comparison of the results with those obtained by a reported method showed good agreement and indicated no significant difference in accuracy and precision.

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