scholarly journals Rooting potential of mature stem cuttings of some forest tree species for vegetative propagation

2013 ◽  
Vol 5 (2) ◽  
pp. 442-446
Author(s):  
E. G. Oboho ◽  
J. N. Iyadi
Keyword(s):  
Somatic Embryogenesis ◽  
Vegetative Propagation ◽  
Callus Formation ◽  
Forest Tree ◽  
Stem Cuttings ◽  
Annona Muricata ◽  
Garcinia Kola ◽  
The Third ◽  
Resource Poor ◽  
Irvingia Gabonensis

The growing of trees could be hindered by seed dormancy problems or unavailability of sufficient quantities of seeds, hence vegetative propagation using simple propagules like stem cuttings is a valued option especially for resource poor farmers. The rooting potential of mature stem cuttings of Gambeya albida, Irvingia gabonensis, Annona muricata, Garcinia kola and Triplochiton scleroxylon was investigated in a humid tent propagator without enhancement by artificial hormones. Propagation indicative parameters were estimated using percentages. G. kola had the highest root-ability indices with cutting survival rate of 85%, callus formation (75%), leaf loss (35%), days to axial bud sprout (55 days), days to leaf loss (23 days) and number of new leaves produced (9) at termination of study. T.scleroxylon had no survivals; an axial bud sprouted at 51 days but withered off by the third day. Other species were between these two extremes. Root-ability potential categorization for the species on the basis of this investigation was: easy to root for: G.kola and A.muricata, moderately easy to root for I.gabonensis and G.albida,unable to root for T.scleroxylon.The use of artificial hormone, juvenile stem cutting and somatic embryogenesis trials has been suggested especially for the moderately easy and unable to root categories.

scholarly journals Studies on Hormonal Effects on Rooting of Marcotting and Stem-Cuttings of Akee Apple (Blighia sapida K. D. Koenig)

2016 ◽  
Vol 8 (4) ◽  
pp. 468-471 ◽  
Author(s):  
Ehoniyotan Olayemi IBUKUN
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Vegetative Propagation ◽  
Callus Formation ◽  
Hormonal Treatment ◽  
The Other ◽  
Naphthalene Acetic Acid ◽  
Naphthaleneacetic Acid ◽  
Stem Cuttings ◽  
Hormonal Treatments

The effect of hormone on the rooting of stem-cuttings and marcotting of akee apple was studied using a combination between Indole -3- Butyric Acid (IBA) and 1- Naphthalene Acetic Acid (NAA). Stem-cuttings from mature akee trees from Challenge, Jalala and Ganmo in Ilorin, were treated with different dilutions of the liquid hormone in the combination of 1.0% Indole-3- butyric acid + 0.5% 1-Naphthaleneacetic acid before propagating them in a non-mist propagator. Marcotting was also carried-out on trees, using the hormonal combination of different dilutions. Observations and the results obtained revealed that the hormonal combination had significant effect on the rooting of stem-cuttings and marcotting. Both marcotting and stem cuttings did not produce at the end of the experiment roots in the absence of the hormonal treatment; a particular aspect was marcotting that initials produced roots. On the other hand, both marcotting and stem cuttings produced roots with the hormonal treatments; more roots were produced using the combination of 2,000 ppm of IBA and 1,000 ppm of NAA, compared with lower concentrations of the hormone mixtures. After callus formation, 2,000 ppm of IBA and 1,000 ppm of NAA combination gave the best results within stem-cuttings. Based on the results obtained, it was concluded that the combination of IBA and NAA in appropriate concentration promoted rooting in Akee apple and therefore are highly valuable for the vegetative propagation of this species through stem cutting and marcotting.

XXVI.—A Comparative Study of the Stem Structure of the Genus Clematis, with special reference to Anatomical Changes induced by Vegetative Propagation

1928 ◽  
Vol 55 (3) ◽  
pp. 643-664 ◽  
Author(s):  
Edith Philip Smith
Keyword(s):  
Vegetative Propagation ◽  
Cell Activity ◽  
Stem Cuttings ◽  
Stem Anatomy ◽  
Anatomical Changes ◽  
The Third ◽  
Regeneration Response ◽  
Place Of Origin ◽  
Insight Into ◽  
Stem Structure

The investigation of which the results are embodied in this paper was undertaken in an attempt to gain an insight into the problem of the propagation of Clematis by means of stem cuttings.In order to be able to interpret the phenomena of regeneration induced by isolating portions of a plant, it is essential to investigate the normal anatomy in some detail. In any severed portion of a plant, the first signs of abnormal cell activity in the tissues depart only slightly from the normal, and unless they can be detected and identified at their initiation, it is impossible to define exactly the time and place of origin of the new growth.Accordingly the first part of the paper will deal with the normal stem-anatomy of the genus. The second part, with the changes in the anatomy resulting from vegetative propagation, and the third part with some experimental modifications of the regeneration-response.

scholarly journals Effect of Root-promoting Products in the Propagation of Organic Olive (Olea europaea L. cv. Cornicabra) Nursery Plants

HortScience ◽  
2008 ◽  
Vol 43 (7) ◽  
pp. 2066-2069 ◽  
Author(s):  
Ana Centeno ◽  
María Gómez-del-Campo
Keyword(s):  
Temperature Control ◽  
Vegetative Propagation ◽  
Treatment Duration ◽  
Sunflower Seed ◽  
Callus Formation ◽  
Stem Cuttings ◽  
Rooted Cuttings ◽  
Dry Extract ◽  
Basal Thickening ◽  
Auxin Effect

Olive cuttings root well using synthetic auxin indole-3-butyric acid (IBA). However, European and North American regulations do not allow the use of synthetic products to obtain organic vegetative propagation materials. In this work, we evaluated different products that could replace IBA in the propagation of olive cv. Cornicabra leafy-stem cuttings. In 2003, six products with a known auxin effect were assessed: IBA, algae extract, brewer's yeast, a bed of sunflower seed, seaweed dry extract (Sm-6 Organico™), and an extract of macerated seeds (Terrabal Organico™). The basal end of cuttings was treated with one of these products and placed on a mist bed with basal temperature control. After 2 months, rooting percentage, number of roots per cutting, number of cuttings with callus formation, and number of cuttings with basal thickening were determined. No significant differences were found in rooting percentage or number of roots per cutting between IBA and Terrabal Organico™ and Sm-6 Organico™. These last products had significantly higher percentage of rooted cuttings without callus formation than IBA. In 2004, a new trial was conducted in which seven treatments were evaluated: IBA applied for 7 s; Terrabal Organico™ applied for 1, 4, and 8 h; and Sm-6 Organico™ applied for 1, 4, and 8 h. No significant differences in rooting percentage or number of roots per cutting were observed between IBA and Terrabal Organico™ applied for 1 h, whereas all the Sm-6 Organico™ treatments had significantly lower rooting percentages than IBA. Both rooting percentage and the percentage of rooted cuttings without callus development decreased significantly as treatment duration with Terrabal Organico™ increased. Therefore, Terrabal Organico™ could produce a toxic effect on cuttings when treatment duration is increased. Thus, Terrabal Organico™ could be a valid alternative to IBA in the propagation of organic olive plants of cv. Cornicabra when applied to the basal end of cuttings for 1 h.

scholarly journals Stem cuttings and substrates in Dovyalis asexual propagation

2018 ◽  
Vol 8 (4) ◽  
pp. 587-595 ◽  
Author(s):  
André Renato Rinaldi ◽  
Fabíola Villa ◽  
Daniel Fernandes da Silva ◽  
Rafael Massahiro Yassue
Keyword(s):  
Butyric Acid ◽  
Vegetative Propagation ◽  
Root Systems ◽  
Stem Cuttings ◽  
Indole Butyric Acid ◽  
Asexual Propagation ◽  
The Third ◽  
Number Of Leaves

The objective of this study was to evaluate the asexual propagation of Dovyalis, through the use of substrates and cuttings. Three experiments were conducted in Marechal Cândido Rondon, Paraná State, Brazil, and the first experiment consisted of three removal positions of the cuttings (apical, middle and basal) x 4 Indole butyric acid (IBA) concentrations (0 mg L-1, 1000 mg L-1, 2000 mg L-1 and 3000 mg L-1); the second experiment evaluated the number of leaves in the cuttings (2, 4 and 6) x 3 cutting sizes (10, 15 and 20 cm); and the third experiment evaluated four substrates for rooting. The experiment was carried out in a randomized blocks design, with the first in a 3x4 and the second in a 3x3 factorial, respectively, containing 4 repetitions of 15 cuttings. The third experiment consisted of 5 repetitions of 15 cuttings. After the IBA treatment, the cuttings were taken to rooting in sandy beds during 70 days. The agronomical variables were evaluated. Basal and middle cuttings resulted in root systems with better development. The use of 1666 mg L-1 of IBA favors the Dovyalis cuttings rooting. Cuttings with 15 and 20 cm with four leaves favors the Dovyalis vegetative propagation. Dovyalis cuttings develop better on substrates containing latosol and vermiculite.

scholarly journals Effect of Cutting Size on the Performance of Exogenous Auxin IBA in Vegetative Propagation of Rubus erythroclados Mart. ex Hook. f.

2021 ◽  
pp. 56-66
Author(s):  
Júlio Tagliari Balestrin ◽  
Talissa Baroni ◽  
Juliana Marcia Rogalski
Keyword(s):  
Vegetative Propagation ◽  
Aerial Part ◽  
Callus Formation ◽  
Root Diameter ◽  
Size Factor ◽  
Stem Cuttings ◽  
Root Number ◽  
Rooted Cuttings ◽  
Shoot Number ◽  
Phytotoxic Effect

Aim: The climbing shrub Rubus erythroclados Mart. ex Hook. f. (amora-verde) has great food (fruits) and medicinal (leaves) potential. However, this species remains understudied. The objective of this study was to evaluate the interaction between cutting size and IBA concentration in the vegetative propagation of R. erythroclados by stem cuttings. Study Design: The experimental design used was completely randomized in a 3x5 factorial combination. The cutting size factor was represented by three levels (10, 15 and 20 cm) and IBA concentration factor by five levels (0, 1,000, 2,000, 3,000 and 4,000 mg L-1). Results: There was an interaction between the factors in 13 of the 19 variables analyzed, being: live cuttings at 30 and 60 days, live cuttings with callus formation, sprouted cuttings at 30, 60 and 90 days, rooted cuttings, shoot number, shoot diameter, root number, root length, length of the largest root and root diameter. Size increase of the R. erythroclados cuttings caused a decrease in the phytotoxic effect of IBA in the development of the aerial part. The effect of IBA in response to rooting induction, in the larger size of R. erythroclados cuttings, required an increase in IBA concentration compared to the cuttings of shorter size. Conclusion: Cuttings of 10 and 15 cm treated with 1,000 mg L-1 of IBA showed greater rooting (20.0%) compared to the other treatments, being, therefore, indicated for the vegetative propagation of R. erythroclados by stem cuttings.

scholarly journals Vegetative propagation of Veronica dahurica and Veronica pusanensis by stem cuttings with auxins

Rhizosphere ◽  
2021 ◽  
Vol 17 ◽  
pp. 100315
Author(s):  
Su-Hyun Kim ◽  
Jin-Ho Kim ◽  
Hye-Jin Oh ◽  
Sang-Yong Kim ◽  
Gang-Uk Suh
Keyword(s):  
Vegetative Propagation ◽  
Stem Cuttings

scholarly journals PELATIHAN PEMBUATAN BIBIT STEK BATANG TANAMAN REHABILITASI DI DESA KARANGSARI KABUPATEN KUNINGAN

2019 ◽  
Vol 2 (02) ◽  
Author(s):  
Yayan Hendrayana ◽  
Agus Yadi Ismail
Keyword(s):  
Community Service ◽  
Vegetative Propagation ◽  
Forest Productivity ◽  
Environmental Conservation ◽  
Stem Cuttings ◽  
Plant Seeds

Ficus spp. is a plant that can play an important role in efforts to increase environmental conservation and forest productivity. One type is beunying (Ficus fistulosa) which is included in plant rehabilitation and restoration. Propagation of the Ficus plant is easiest to do by vegetative or stem cuttings. Therefore community service offers a solution in the form of making rehabilitation plant seeds through cuttings of Ficus fistulosa tree trunks. The hope is that by utilizing the rehabilitation tree that tastes in the Pasirbatang Block, this tourism mobilizing farmer group can produce rehabilitation plant seeds and increase the opinions of its members.Keywords: Vegetative propagation, training, empowerment.��AbstrakFicus spp. merupakan tanaman yang dapat berperan penting dalam upaya peningkatan konservasi lingkungan maupun produktivitas hutan. Salah satu jenis nya adalah beunying (Ficus fistulosa) merupakan yang termasuk ke dalam tanaman rehabilitasi dan restorasi. Perbanyakan tanaman Ficus yang paling mudah dilakukan adalah dengan cara vegetatif atau stek batang. Oleh karena itu pengabdian kepada masyarakat menawarkan solusi berupa cara pembuatan bibit tanaman rehabilitasi melalui stek batang pohon Ficus fistulosa. Harapannya adalah dengan memanfaatkan pohon rehabilitasi yang berasa di Blok Pasirbatang ini kelompok tani penggerak pariwisata ini dapat menghasilkan bibit tanaman rehabilitasi serta meningkatkan pendapatn para anggotanya.Kata kunci : Perbanyakan vegetatif, pelatihan, pemberdayaan

scholarly journals The effect of auxin 2,4-D and cytokinin 2-ip on direct somatic embryogenesis formation of Coffea arabica L. leaf explant

2011 ◽  
Vol 27 (2) ◽  
Author(s):  
Rina Arimarsetiowati
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Coffea Arabica ◽  
Callus Formation ◽  
Culture Technique ◽  
Plant Production ◽  
Direct Somatic Embryogenesis ◽  
Arabica Coffee ◽  
Randomized Design ◽  
Coffea Arabica L

One of the propagation technique for coffee plant production is tissue culture. Tissue culture technique for Coffea arabica L. faces some problems, mainly in the planlet formation regenerated from explants. The objective of this experiment was to examine the effect 2,4-D and 2-ip combination on the formation of direct somatic embryogenesis of Coffea arabica L. in leaves explant. Auxin (2,4-D) and cytokinin (2-ip) concentrations of, respectively, 1; 5 µM and 5; 10; 15; 20 were used as treatments. This research was conducted using completely randomized design with 10 replications. Observation to induce somatic embryos was done by quantitatively on number of callus from explant and number of embryogenic callus. Beside that, observation by qualitative descriptive was also done on deve lopment of embryogenesis. The results showed that Arabica coffee leaves explant of AS 2K clones could be induced in all medium combination except 5µM 2,4-D and 20µM 2-ip combination. Arabica coffee leaves explant of S 795, Sigararutang and AS 1 varieties could be induced in all medium combination. The highest frequency of callus formation was found in AS 2K, Sigararutang and AS 1 varieties on medium containing 1µM 2,4-D in combination with 10µM 2-ip, whereas for the S 795 variety on medium containing 5µM 2,4-D in combination with 10µM 2-ip. The highest frequency of embriogenic callus in all Arabica coffee variety could be reached on medium containing 5µM 2,4-D in combination with 15µM 2-ip. Key words : Coffea arabica L., somatic embryogenesis, 2,4-D, 2-ip, tissue culture, leaves, callus embryogenic.

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