scholarly journals Effect of Root-promoting Products in the Propagation of Organic Olive (Olea europaea L. cv. Cornicabra) Nursery Plants

HortScience ◽  
2008 ◽  
Vol 43 (7) ◽  
pp. 2066-2069 ◽  
Author(s):  
Ana Centeno ◽  
María Gómez-del-Campo
Keyword(s):  
Temperature Control ◽  
Vegetative Propagation ◽  
Treatment Duration ◽  
Sunflower Seed ◽  
Callus Formation ◽  
Stem Cuttings ◽  
Rooted Cuttings ◽  
Dry Extract ◽  
Basal Thickening ◽  
Auxin Effect

Olive cuttings root well using synthetic auxin indole-3-butyric acid (IBA). However, European and North American regulations do not allow the use of synthetic products to obtain organic vegetative propagation materials. In this work, we evaluated different products that could replace IBA in the propagation of olive cv. Cornicabra leafy-stem cuttings. In 2003, six products with a known auxin effect were assessed: IBA, algae extract, brewer's yeast, a bed of sunflower seed, seaweed dry extract (Sm-6 Organico™), and an extract of macerated seeds (Terrabal Organico™). The basal end of cuttings was treated with one of these products and placed on a mist bed with basal temperature control. After 2 months, rooting percentage, number of roots per cutting, number of cuttings with callus formation, and number of cuttings with basal thickening were determined. No significant differences were found in rooting percentage or number of roots per cutting between IBA and Terrabal Organico™ and Sm-6 Organico™. These last products had significantly higher percentage of rooted cuttings without callus formation than IBA. In 2004, a new trial was conducted in which seven treatments were evaluated: IBA applied for 7 s; Terrabal Organico™ applied for 1, 4, and 8 h; and Sm-6 Organico™ applied for 1, 4, and 8 h. No significant differences in rooting percentage or number of roots per cutting were observed between IBA and Terrabal Organico™ applied for 1 h, whereas all the Sm-6 Organico™ treatments had significantly lower rooting percentages than IBA. Both rooting percentage and the percentage of rooted cuttings without callus development decreased significantly as treatment duration with Terrabal Organico™ increased. Therefore, Terrabal Organico™ could produce a toxic effect on cuttings when treatment duration is increased. Thus, Terrabal Organico™ could be a valid alternative to IBA in the propagation of organic olive plants of cv. Cornicabra when applied to the basal end of cuttings for 1 h.

scholarly journals Effect of Cutting Size on the Performance of Exogenous Auxin IBA in Vegetative Propagation of Rubus erythroclados Mart. ex Hook. f.

2021 ◽  
pp. 56-66
Author(s):  
Júlio Tagliari Balestrin ◽  
Talissa Baroni ◽  
Juliana Marcia Rogalski
Keyword(s):  
Vegetative Propagation ◽  
Aerial Part ◽  
Callus Formation ◽  
Root Diameter ◽  
Size Factor ◽  
Stem Cuttings ◽  
Root Number ◽  
Rooted Cuttings ◽  
Shoot Number ◽  
Phytotoxic Effect

Aim: The climbing shrub Rubus erythroclados Mart. ex Hook. f. (amora-verde) has great food (fruits) and medicinal (leaves) potential. However, this species remains understudied. The objective of this study was to evaluate the interaction between cutting size and IBA concentration in the vegetative propagation of R. erythroclados by stem cuttings. Study Design: The experimental design used was completely randomized in a 3x5 factorial combination. The cutting size factor was represented by three levels (10, 15 and 20 cm) and IBA concentration factor by five levels (0, 1,000, 2,000, 3,000 and 4,000 mg L-1). Results: There was an interaction between the factors in 13 of the 19 variables analyzed, being: live cuttings at 30 and 60 days, live cuttings with callus formation, sprouted cuttings at 30, 60 and 90 days, rooted cuttings, shoot number, shoot diameter, root number, root length, length of the largest root and root diameter. Size increase of the R. erythroclados cuttings caused a decrease in the phytotoxic effect of IBA in the development of the aerial part. The effect of IBA in response to rooting induction, in the larger size of R. erythroclados cuttings, required an increase in IBA concentration compared to the cuttings of shorter size. Conclusion: Cuttings of 10 and 15 cm treated with 1,000 mg L-1 of IBA showed greater rooting (20.0%) compared to the other treatments, being, therefore, indicated for the vegetative propagation of R. erythroclados by stem cuttings.

scholarly journals Studies on Hormonal Effects on Rooting of Marcotting and Stem-Cuttings of Akee Apple (Blighia sapida K. D. Koenig)

2016 ◽  
Vol 8 (4) ◽  
pp. 468-471 ◽  
Author(s):  
Ehoniyotan Olayemi IBUKUN
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Vegetative Propagation ◽  
Callus Formation ◽  
Hormonal Treatment ◽  
The Other ◽  
Naphthalene Acetic Acid ◽  
Naphthaleneacetic Acid ◽  
Stem Cuttings ◽  
Hormonal Treatments

The effect of hormone on the rooting of stem-cuttings and marcotting of akee apple was studied using a combination between Indole -3- Butyric Acid (IBA) and 1- Naphthalene Acetic Acid (NAA). Stem-cuttings from mature akee trees from Challenge, Jalala and Ganmo in Ilorin, were treated with different dilutions of the liquid hormone in the combination of 1.0% Indole-3- butyric acid + 0.5% 1-Naphthaleneacetic acid before propagating them in a non-mist propagator. Marcotting was also carried-out on trees, using the hormonal combination of different dilutions. Observations and the results obtained revealed that the hormonal combination had significant effect on the rooting of stem-cuttings and marcotting. Both marcotting and stem cuttings did not produce at the end of the experiment roots in the absence of the hormonal treatment; a particular aspect was marcotting that initials produced roots. On the other hand, both marcotting and stem cuttings produced roots with the hormonal treatments; more roots were produced using the combination of 2,000 ppm of IBA and 1,000 ppm of NAA, compared with lower concentrations of the hormone mixtures. After callus formation, 2,000 ppm of IBA and 1,000 ppm of NAA combination gave the best results within stem-cuttings. Based on the results obtained, it was concluded that the combination of IBA and NAA in appropriate concentration promoted rooting in Akee apple and therefore are highly valuable for the vegetative propagation of this species through stem cutting and marcotting.

scholarly journals The prognostication of the rooting ability of apple stem cuttings by indices of seasonal growth of shoots

2021 ◽  
Vol 89-90 ◽  
pp. 101-109
Author(s):  
Alla Konopelko
Keyword(s):  
Callus Formation ◽  
Average Length ◽  
Total Duration ◽  
Biologically Active ◽  
Stem Cuttings ◽  
Seasonal Growth ◽  
Strong Negative Correlation ◽  
Rooted Cuttings ◽  
Rooting Ability ◽  
Short Period

The study involved seven species and cultivars of ornamental apple trees including Malus × floribunda, M. halliana, M. niedzwetzkyana, M. × purpurea, M. × purpurea ‘Ola’, M. × purpurea ‘Royalty’, and M. × purpurea ‘Selkirk’. The average value of increment, average length, and duration of shoots’ growth were determined to characterize the seasonal growth of Malus shoots. The percentages of rooted cuttings, callus formation without roots, and unviable cuttings were registered for each investigated taxon. Statistical analysis was performed in Microsoft Excel 2007 following Zaitsev (1990), and Atramentova & Utevskaya (2014).The total duration of shoots growth of the studied species and varieties ranged from 72 (M. × purpurea ‘Ola’) to 118 days (M. niedzwetzkyana); annual shoots reached a length from 213.75 mm (M. × purpurea) to 448.75 mm (M. niedzwetzkyana); the average increment of shoots ranged from 3.90 mm (M. × purpurea ‘Ola’) to 14.70 mm (M. floribunda).The rooting ability of Malus stem cuttings was limited to a reasonably short period of cutting procedure and depended on the application of biologically active substances, their concentrations, and complexes. The highest rooting rate (33.33 %) was observed in M. × purpurea ‘Ola’ after the treatment of its cuttings by 0.6 % indole-3-butyric acid (IBA) at the end of June. A slightly lower rooting rate (20.00 %) was observed in M. halliana cuttings treated by 0.4 % IBA and M. × purpurea ‘Selkirk’ cuttings treated by Podkorzen AB aqua at the end of June. Finally, 7.69 % of M. × floribunda cuttings were rooted with Podkorzen AB aqua at the beginning of July.The prediction of the rooting ability of Malus stem cuttings was confirmed by a strong negative correlation between the percentage of rooted cuttings and the duration of shoots’ growth (r = –0.88). Hence, the percentage of rooted cuttings increases with decreasing duration of shoots growth, depending on the genotype.

scholarly journals Vegetative Propagation of Gabirobeira Associate to Indolbutyric Acid in Different Seasons

2019 ◽  
Vol 11 (7) ◽  
pp. 187
Author(s):  
Lasara K. F. Souza ◽  
Lana L. L. Dias ◽  
Moab A. Barbosa ◽  
Diego I. Rocha ◽  
Edésio F. Reis ◽  
...  
Keyword(s):  
Vegetative Propagation ◽  
Stem Cuttings ◽  
Spring Season ◽  
Rooted Cuttings ◽  
Randomized Design ◽  
Different Seasons ◽  
Completely Randomized Design ◽  
Adventitious Rhizogenesis

The propagation of gabirobeira is carried out by seeds. However, the recalcitrance and not tolerance of the seeds to the storage compromise the formation of seedlings. Thus, the propagation by cuttings can be an option, being able to provide significant results in different seasons. The objective of this work was to evaluate the vegetative propagation by stem cuttings of two species of gabirobeira (Campomanesia adamantium and Campomanesia pubescens) associate to indolbutyric acid (IBA) at differents seasons of the year. Cuttings of the two species were obtained in the 4 seasons and concentrations of 0, 2000, 4000, 6000 and 8000 mg L-1 of IBA were used, by immersion in the solution for 15 seconds and sequentially staked on trays containing a sand substrate. Sixty days after experiments were set it was evaluated the percentage of cuttings with shoots, live cuttings, dead cuttings, cuttings with calluses and rooted cuttings. A completely randomized design with 5 treatments, 4 replicates and 8 cuttings per plot was adopted. During the whole experiment, the greenhouse, substrate and cutting leaves temperature was monitored. The use of IBA did not promote adventitious rhizogenesis, but provided a high percentage of survival for the species Campomanesia adamantium and Campomanesia pubescens by stem cuttings during the spring season.

scholarly journals Vegetative Propagation of Peltophorum Petrocarpum (DC.) Backer ex K.Heyne a Multipurpose Tree

2014 ◽  
Vol 1 (2) ◽  
pp. 79-84
Author(s):  
Fadwa Mohammed Ahmed Ali ◽  
Yahia Hamid Ali Elbasheer
Keyword(s):  
Growth Performance ◽  
Biomass Production ◽  
Butyric Acid ◽  
Vegetative Propagation ◽  
Large Scale ◽  
Tree Planting ◽  
Research Centre ◽  
Stem Cuttings ◽  
Rooted Cuttings ◽  
Mother Plants

This paper describes a successful protocol of vegetative propagation by using stem cuttings of mature Peltophorum petrocarpum plants. The experiment was conducted in the nursery of the Forestry Research Centre at Soba during November 2011and April 2012. The socioeconomic importance of P. petrocarpum comes from its multipurpose uses and there is a demand in production of its seed for tree planting and domestication activities. Hence there is a need for efficient and economically feasible propagation method of P. pterocarpum. Therefore the objective of study is to develop a vegetative propagation protocol by using rooted stem cuttings; and to determine the effects of the rooting hormone indole-3-butyric acid (IBA) on rooting potentiality of stem cuttings of mature mother plants. The result revealed a significant effect (p<0.001) of IBA concentrations on rooting percentage and growth performance of stem cuttings. The highest rooting percent was obtained with 200ppm IBA which was not significantly different from 100ppm, but significantly different from 50 and 0.0ppm. The effect of IBA concentrations on root lengths and of roots per cutting was significantly different from 0.0 and 50ppm treatments. Although 100ppm IBA gave the tallest root (4.6 cm) but it was not significantly different from 200ppm (4.3 cm). Survival of rooted cuttings after transplanting from the propagator was 100% and 97% with 100 and 200mg IBA respectively. Accordingly the conclusion reached is that rooted cuttings can be used successfully for obtaining health, uniform plantations of P. petrocarpum for large-scale, biomass production and tree planting programmes.   

scholarly journals Cutting Type and IBA Treatment Duration Affect Teucrium fruticans Adventitious Root Quality

2014 ◽  
Vol 42 (2) ◽  
pp. 478-481 ◽  
Author(s):  
Leo SABATINO ◽  
Fabio D’ANNA ◽  
Giovanni IAPICHINO
Keyword(s):  
Acid Solution ◽  
Butyric Acid ◽  
Root Development ◽  
Root Length ◽  
Treatment Duration ◽  
Acid Treatment ◽  
Stem Cuttings ◽  
Root Number ◽  
Rooted Cuttings ◽  
Root Quality

Root development of stem cuttings of Silver Germander (Teucrium fruticans) was investigated in relation to cutting type and indole-3-butyric acid (IBA) treatment. Terminal cuttings of a clone grown in Sicily were trimmed to three types: terminal cuttings with apex (TWA), terminal cuttings without apex (TWOA) or sub-terminal cuttings (ST). To verify the cutting response to exogenous auxin, cuttings were dipped to a 2.0 cm depth in a 0.5% indole-3-butyric acid solution for 0, 5 or 7 minutes. Overall percent survival was 97 to 98%. Rooting percent, root number and root length were affected by cutting type and indole-3-butyric acid treatment. In general, TWA cuttings demonstrated a higher capacity to form roots than cuttings without apex (TWOA and ST cuttings). In absence of indole-3-butyric acid treatment, TWA and ST cuttings gave higher rooting percentages than TWOA cuttings. Exposing cuttings to indole-3-butyric acid improved percent rooting, number of roots per cutting and root length. The best results in terms of rooting percentage and root number per cuttings were obtained with TWA cuttings in combination with 7 min indole-3-butyric acid basal dip. However, cuttings taken farther down the stem, such as sub-terminal cuttings gave satisfactory rooting performance as well. We suggest that the use of all cutting types tested associated to indole-3-butyric acid basal dip for 5 or 7 min may be beneficial to propagators wishing to produce T. fruticans rooted cuttings with well-developed root system.

scholarly journals Early Season Softwood Cuttings Effective for Vegetative Propagation of Juglans cinerea

HortScience ◽  
2002 ◽  
Vol 37 (4) ◽  
pp. 697-700 ◽  
Author(s):  
Paula M. Pijut ◽  
Melanie J. Moore
Keyword(s):  
Butyric Acid ◽  
Cold Storage ◽  
Vegetative Propagation ◽  
Potassium Salt ◽  
Root Induction ◽  
First Flush ◽  
Stem Cuttings ◽  
Rooted Cuttings ◽  
Juglans Cinerea ◽  
Hardwood Species

Juglans cinerea L. (butternut) is a hardwood species valued for its wood and edible nuts. Information on the vegetative propagation of this species is currently unavailable. Our objective was to determine the conditions necessary for successful stem-cutting propagation of butternut. In 1999 and 2000, 10 trees (each year) were randomly selected from a 5- and 6-year-old butternut plantation located in Rosemount, Minn. Hardwood stem cuttings were collected in March, April, and May. Softwood cuttings were collected in June and July. K-IBA at 0, 29, or 62 mm in water and IBA at 0, 34, or 74 mm in 70% ethanol were tested for root induction on cuttings. The basal end of cuttings were dipped in a treatment solution for 10 to 15 seconds, potted in a peat: perlite mixture, and placed in a mist bed for 5 to 8 weeks. Rooted cuttings were gradually hardened off from the mist bed, allowed to initiate new growth, over-wintered in a controlled cold-storage environment, and then outplanted to the field. For hardwood cuttings, rooting was greatest for those taken in mid-May (branches flushed out), 22% with 62 mm K-IBA and 28% with 74 mm IBA. Softwood cuttings rooted best when taken in June (current season's first flush of new growth or softwood growth 40 cm or greater) and treated with 62 mm K-IBA (77%) or 74 mm IBA (88%). For 1999, 31 out of 51 rooted softwood cuttings (60.8%) survived overwintering in cold storage and acclimatization to the field. For 2000, 173 out of 186 rooted softwood cuttings (93%) survived overwintering and acclimatization to the field. Chemical names used: indole-3-butyric acid-potassium salt (K-IBA); indole-3-butyric acid (IBA).

scholarly journals Rooting potential of mature stem cuttings of some forest tree species for vegetative propagation

2013 ◽  
Vol 5 (2) ◽  
pp. 442-446
Author(s):  
E. G. Oboho ◽  
J. N. Iyadi
Keyword(s):  
Somatic Embryogenesis ◽  
Vegetative Propagation ◽  
Callus Formation ◽  
Forest Tree ◽  
Stem Cuttings ◽  
Annona Muricata ◽  
Garcinia Kola ◽  
The Third ◽  
Resource Poor ◽  
Irvingia Gabonensis

The growing of trees could be hindered by seed dormancy problems or unavailability of sufficient quantities of seeds, hence vegetative propagation using simple propagules like stem cuttings is a valued option especially for resource poor farmers. The rooting potential of mature stem cuttings of Gambeya albida, Irvingia gabonensis, Annona muricata, Garcinia kola and Triplochiton scleroxylon was investigated in a humid tent propagator without enhancement by artificial hormones. Propagation indicative parameters were estimated using percentages. G. kola had the highest root-ability indices with cutting survival rate of 85%, callus formation (75%), leaf loss (35%), days to axial bud sprout (55 days), days to leaf loss (23 days) and number of new leaves produced (9) at termination of study. T.scleroxylon had no survivals; an axial bud sprouted at 51 days but withered off by the third day. Other species were between these two extremes. Root-ability potential categorization for the species on the basis of this investigation was: easy to root for: G.kola and A.muricata, moderately easy to root for I.gabonensis and G.albida,unable to root for T.scleroxylon.The use of artificial hormone, juvenile stem cutting and somatic embryogenesis trials has been suggested especially for the moderately easy and unable to root categories.

scholarly journals Vegetative propagation of Veronica dahurica and Veronica pusanensis by stem cuttings with auxins

Rhizosphere ◽  
2021 ◽  
Vol 17 ◽  
pp. 100315
Author(s):  
Su-Hyun Kim ◽  
Jin-Ho Kim ◽  
Hye-Jin Oh ◽  
Sang-Yong Kim ◽  
Gang-Uk Suh
Keyword(s):  
Vegetative Propagation ◽  
Stem Cuttings
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