scholarly journals Effect of Cutting Size on the Performance of Exogenous Auxin IBA in Vegetative Propagation of Rubus erythroclados Mart. ex Hook. f.

2021 ◽  
pp. 56-66
Author(s):  
Júlio Tagliari Balestrin ◽  
Talissa Baroni ◽  
Juliana Marcia Rogalski
Keyword(s):  
Vegetative Propagation ◽  
Aerial Part ◽  
Callus Formation ◽  
Root Diameter ◽  
Size Factor ◽  
Stem Cuttings ◽  
Root Number ◽  
Rooted Cuttings ◽  
Shoot Number ◽  
Phytotoxic Effect

Aim: The climbing shrub Rubus erythroclados Mart. ex Hook. f. (amora-verde) has great food (fruits) and medicinal (leaves) potential. However, this species remains understudied. The objective of this study was to evaluate the interaction between cutting size and IBA concentration in the vegetative propagation of R. erythroclados by stem cuttings. Study Design: The experimental design used was completely randomized in a 3x5 factorial combination. The cutting size factor was represented by three levels (10, 15 and 20 cm) and IBA concentration factor by five levels (0, 1,000, 2,000, 3,000 and 4,000 mg L-1). Results: There was an interaction between the factors in 13 of the 19 variables analyzed, being: live cuttings at 30 and 60 days, live cuttings with callus formation, sprouted cuttings at 30, 60 and 90 days, rooted cuttings, shoot number, shoot diameter, root number, root length, length of the largest root and root diameter. Size increase of the R. erythroclados cuttings caused a decrease in the phytotoxic effect of IBA in the development of the aerial part. The effect of IBA in response to rooting induction, in the larger size of R. erythroclados cuttings, required an increase in IBA concentration compared to the cuttings of shorter size. Conclusion: Cuttings of 10 and 15 cm treated with 1,000 mg L-1 of IBA showed greater rooting (20.0%) compared to the other treatments, being, therefore, indicated for the vegetative propagation of R. erythroclados by stem cuttings.

scholarly journals Effect of Root-promoting Products in the Propagation of Organic Olive (Olea europaea L. cv. Cornicabra) Nursery Plants

HortScience ◽  
2008 ◽  
Vol 43 (7) ◽  
pp. 2066-2069 ◽  
Author(s):  
Ana Centeno ◽  
María Gómez-del-Campo
Keyword(s):  
Temperature Control ◽  
Vegetative Propagation ◽  
Treatment Duration ◽  
Sunflower Seed ◽  
Callus Formation ◽  
Stem Cuttings ◽  
Rooted Cuttings ◽  
Dry Extract ◽  
Basal Thickening ◽  
Auxin Effect

Olive cuttings root well using synthetic auxin indole-3-butyric acid (IBA). However, European and North American regulations do not allow the use of synthetic products to obtain organic vegetative propagation materials. In this work, we evaluated different products that could replace IBA in the propagation of olive cv. Cornicabra leafy-stem cuttings. In 2003, six products with a known auxin effect were assessed: IBA, algae extract, brewer's yeast, a bed of sunflower seed, seaweed dry extract (Sm-6 Organico™), and an extract of macerated seeds (Terrabal Organico™). The basal end of cuttings was treated with one of these products and placed on a mist bed with basal temperature control. After 2 months, rooting percentage, number of roots per cutting, number of cuttings with callus formation, and number of cuttings with basal thickening were determined. No significant differences were found in rooting percentage or number of roots per cutting between IBA and Terrabal Organico™ and Sm-6 Organico™. These last products had significantly higher percentage of rooted cuttings without callus formation than IBA. In 2004, a new trial was conducted in which seven treatments were evaluated: IBA applied for 7 s; Terrabal Organico™ applied for 1, 4, and 8 h; and Sm-6 Organico™ applied for 1, 4, and 8 h. No significant differences in rooting percentage or number of roots per cutting were observed between IBA and Terrabal Organico™ applied for 1 h, whereas all the Sm-6 Organico™ treatments had significantly lower rooting percentages than IBA. Both rooting percentage and the percentage of rooted cuttings without callus development decreased significantly as treatment duration with Terrabal Organico™ increased. Therefore, Terrabal Organico™ could produce a toxic effect on cuttings when treatment duration is increased. Thus, Terrabal Organico™ could be a valid alternative to IBA in the propagation of organic olive plants of cv. Cornicabra when applied to the basal end of cuttings for 1 h.

scholarly journals MINICUTTING OF CHARDONNAY GRAPEVINE AND SO4 GRAPEVINE ROOTSTOCK

2019 ◽  
Vol 21 (3) ◽  
pp. 323-332
Author(s):  
Daniele Camargo Nascimento ◽  
Maximiliano Dini ◽  
Roseane Maidana Moreira ◽  
Norton Victor Sampaio ◽  
Márcia Wulff Schuch
Keyword(s):  
Experimental Design ◽  
Vegetative Propagation ◽  
Callus Formation ◽  
Average Length ◽  
Root Number ◽  
Indolebutyric Acid ◽  
Shoot Number ◽  
Four Levels

The use of grapevine minicutting is an alternative for the production of quality plants. This technique has been gaining ground as one of the most used forms of vegetative propagation. The objective of this study was to evaluate the rooting of herbaceous minicuttings of Chardonnay grapevine and SO4 grapevine rootstock by using different concentrations of indolebutyric acid (IBA) to form a clonal mini-garden. The experimental design was randomized blocks with four levels of IBA concentrations (0, 500, 1000 and 1500 mg L-1). The minicuttings remained in trays containing vermiculite and kept in a micro sprinkler chamber. After 60 days, the percentage of survival, rooting and callus formation, root number and average length, shoot number and average length were evaluated. For Chardonnay, the means of survival and rooting varied between 93 and 100% in all treatments. In relation to SO4, the IBA concentration of 1500 mg L-1 negatively affected the minicuttings survival and rooting. It is possible to propagate Chardonnay grapevine and SO4 grapevine rootstock through herbaceous minicutting, with no need of apply IBA for the rooting.

scholarly journals Propagation of Ulmus parvifolia ‘Emerald Prairie’ by Stem Cuttings

2004 ◽  
Vol 22 (2) ◽  
pp. 55-57
Author(s):  
Jason J. Griffin ◽  
Kenneth R. Schroeder
Keyword(s):  
Plant Growth ◽  
Growth Stage ◽  
Stem Cuttings ◽  
Root Number ◽  
Stock Plant ◽  
Rooted Cuttings ◽  
Rooted Cutting ◽  
Ulmus Parvifolia ◽  
Survival And Growth ◽  
Terminal Shoot

Abstract Stem cuttings of Ulmus parvifolia Jacq. ‘Emerald Prairie’ (‘Emerald Prairie’ lacebark elm), consisting of 7.5 to 10 cm (3 to 4 in) terminal shoot portions, were collected four times throughout the year from mature stock plants and treated with the potassium (K) salt of indole-3-butyric acid (K-IBA) ranging from 0 to 20,000 ppm (2.0%). Rooting percentages were affected greatly by K-IBA treatment and stock plant growth stage. Little to no rooting was observed without K-IBA application, whereas overall rooting for treated softwood, early semi-hardwood, late semi-hardwood, and hardwood, cuttings was 92, 86, 87, and 8%, respectively. Mean root number varied by growth stage and K-IBA concentration. Higher concentrations of K-IBA increased the number of roots on rooted cuttings except for hardwood cuttings. In general, rooting percentage and mean root number were highest at the softwood stage with 15,000 or 20,000 ppm (1.5 or 2.0%) K-IBA, resulting in 97% rooting and 15 or 22 roots per rooted cutting, respectively. However, leaf abscission was high on softwood cuttings. Semi-hardwood cuttings achieved similar rooting percentages and may exhibit better survival and growth due to greater leaf retention.

scholarly journals Indolebutyric acid on boxwood propagation by stem cuttings

2018 ◽  
Vol 24 (4) ◽  
pp. 347-352 ◽  
Author(s):  
Leandro Marcolino Vieira ◽  
Silvano Kruchelski ◽  
Erik Nunes Gomes ◽  
Katia Christina Zuffellato-Ribas
Keyword(s):  
Statistical Difference ◽  
Callus Formation ◽  
Adventitious Rooting ◽  
Commercial Production ◽  
Stem Cuttings ◽  
Root Number ◽  
Indolebutyric Acid ◽  
Significant Percentage ◽  
Root Numbers ◽  
Buxus Sempervirens

Buxus sempervirens L., popularly known as boxwood, is a woody shrub widely used in gardening and landscaping. One of the most important factors for B. sempervirens commercial production is its propagation. The objective of this study was to evaluate the effect of indolebutyric acid (IBA) concentrations on the adventitious rooting of semihardwood stem cuttings of the species. Branches with new shoots were collected from stock plants with approximately 17 years of growth. Stem cuttings of six centimeters in length with two leaves in the upper region were made with a straight cut at the apex and a bevel cut at the base. Cuttings bases were immersed for 10 seconds in hydroalcoholic solutions (50% v v-1) with IBA at 0, 1500, 3,000 or 6,000 mg L-1. The cuttings were evaluated at 64 and 116 days after planting. Rooting, mortality, callus formation and sprouting percentages as well as root number and root length were measured. At 64 days, no significant percentage of rooting was observed in B. sempervirens stem cuttings and sprouting and callus formation were not significantly affected by the treatments. Keeping the cuttings in the greenhouse for 116 days led to rooting rates up to a 97.5%, with no statistical difference among treatments. Root lengths and root numbers did not differ as a function of treatments. The required period for B. sempervirens stem cuttings rooting is around 116 days; IBA application does not affect rooting, survival or sprouting of B. sempervirens stem cuttings.

scholarly journals Studies on Hormonal Effects on Rooting of Marcotting and Stem-Cuttings of Akee Apple (Blighia sapida K. D. Koenig)

2016 ◽  
Vol 8 (4) ◽  
pp. 468-471 ◽  
Author(s):  
Ehoniyotan Olayemi IBUKUN
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Vegetative Propagation ◽  
Callus Formation ◽  
Hormonal Treatment ◽  
The Other ◽  
Naphthalene Acetic Acid ◽  
Naphthaleneacetic Acid ◽  
Stem Cuttings ◽  
Hormonal Treatments

The effect of hormone on the rooting of stem-cuttings and marcotting of akee apple was studied using a combination between Indole -3- Butyric Acid (IBA) and 1- Naphthalene Acetic Acid (NAA). Stem-cuttings from mature akee trees from Challenge, Jalala and Ganmo in Ilorin, were treated with different dilutions of the liquid hormone in the combination of 1.0% Indole-3- butyric acid + 0.5% 1-Naphthaleneacetic acid before propagating them in a non-mist propagator. Marcotting was also carried-out on trees, using the hormonal combination of different dilutions. Observations and the results obtained revealed that the hormonal combination had significant effect on the rooting of stem-cuttings and marcotting. Both marcotting and stem cuttings did not produce at the end of the experiment roots in the absence of the hormonal treatment; a particular aspect was marcotting that initials produced roots. On the other hand, both marcotting and stem cuttings produced roots with the hormonal treatments; more roots were produced using the combination of 2,000 ppm of IBA and 1,000 ppm of NAA, compared with lower concentrations of the hormone mixtures. After callus formation, 2,000 ppm of IBA and 1,000 ppm of NAA combination gave the best results within stem-cuttings. Based on the results obtained, it was concluded that the combination of IBA and NAA in appropriate concentration promoted rooting in Akee apple and therefore are highly valuable for the vegetative propagation of this species through stem cutting and marcotting.

scholarly journals Sodium Cellulose Glycolate as a Thickening Agent for Liquid Auxin Formulations can Enhance Rooting of Stem Cuttings

2007 ◽  
Vol 25 (3) ◽  
pp. 126-130
Author(s):  
Eugene K. Blythe ◽  
Jeff L. Sibley
Keyword(s):  
Shoot Growth ◽  
Extended Period ◽  
Stem Cuttings ◽  
Root Number ◽  
Hedera Helix ◽  
Rooted Cuttings ◽  
Thickening Agent ◽  
Auxin Concentration ◽  
Ilex Vomitoria ◽  
Hibiscus Syriacus

Abstract Stem cuttings of Abelia ‘Edward Goucher’, Buxus sinica var. insularis ‘Wintergreen’, Hedera helix, Hibiscus syriacus ‘Collie Mullens’, Ilex vomitoria ‘Nana’, Juniperus conferta ‘Blue Pacific’, and Rosa ‘Red Cascade’ received a basal quick-dip in solutions of Dip ‘N Grow at concentrations of 0 + 0, 50 + 25, 250 + 125, 500 + 250, 750 + 375, and 1000 + 500 ppm IBA + NAA prepared with and without 13.5 g/liter sodium cellulose glycolate (SCG) as a thickening agent. Cuttings of Ilex vomitoria ‘Nana’ exhibited increased rooting with increasing auxin concentration with inclusion of SCG. The other six taxa exhibited similar rooting percentages among all treatments, but exhibited an increase in root number and/or total root length with inclusion of SCG. Initial shoot growth on rooted cuttings of Abelia ‘Edward Goucher’ showed some reduction with increasing auxin concentration with inclusion of SCG, suggesting cuttings absorbed more auxin from solutions containing SCG owing to their extended period of exposure to the auxin. Otherwise, there were no negative responses to solutions containing SCG. The greater viscosity of solutions prepared with SCG can help reduce the possibility of spillage and evaporation of alcohol during use of the auxin solutions.

scholarly journals Shoot Proliferation and Growth of Gardenia (Gardenia jasminoides Ellis) in Response to pH and Sugar Concentrations in Vitro

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 461B-461
Author(s):  
Karim H. Al-Juboory ◽  
L.K. Al-Amiry ◽  
M.M Kushad
Keyword(s):  
Shoot Proliferation ◽  
Dry Weight ◽  
Shoot Length ◽  
Stem Cuttings ◽  
Root Number ◽  
Fresh Weight ◽  
Gardenia Jasminoides ◽  
Shoot Number ◽  
Effects Of Ph

Stem cuttings of greenhouse-grown gardenia (Gardenia jasminoides Ellis) were surface-sterilized and planted on modified Murashige and Skoog (MS) medium supplemented with 2.0 mg/L (BA) and 0.5 mg/L (NAA). This study examines the effects of pH and various sugars on the growth and proliferation of in vitro-cultured gardenia. The highest average shoot number and shoot length were obtained at pH 4.0 to 5.0. In determining the effect of sugars on shoot proliferation, our findings indicated that sucrose and glucose at 30 and 40 g/L, respectively, produced a higher shoot number when compared to fructose and xylose. In addition, sucrose and glucose produced the highest root number, root length, and rooting percentage, while fructose and xylose had no effect on rooting. Sucrose and fructose produced more calli in comparison to other sugars. Xylose at 40 g/L produced the highest dry weight (18.5%), while xylose at 10 g/L produced the highest fresh weight (94.4%).

scholarly journals Effect of Indole-3-Butyric Acid on Clonal Propagation of Swietenia macrophylla through Branch Cutting

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Md. Salim Azad ◽  
Md. Abdul Matin
Keyword(s):  
Overall Survival ◽  
Butyric Acid ◽  
Clonal Propagation ◽  
Root Number ◽  
Swietenia Macrophylla ◽  
Rooted Cuttings ◽  
Survival Percentage ◽  
Shoot Number ◽  
Number Of Leaves ◽  
Branch Cutting

The study discloses the scopes of clonal propagation Swietenia macrophylla through branch cutting treated with IBA. A total of four hundred cuttings were used with four replications to assess the rooting ability. The study exposed significant (p<0.5) difference of rooted cuttings among the treatments. The highest (62.51%) rooting percent was observed in cutting with 0.4% IBA treatment. In addition, root number and its length per cutting were increased with increasing IBA concentration (p<0.5). The experiment showed significant (p<0.5) difference of sprouting among the treatments. The highest (67.27%) percent of sprouting was observed in cuttings with 0.4% solution, which showed a similar fashion of percent of rooting. The study also showed significant (p<0.5) difference of shoot number per cutting, the length of the longest shoot, and number of leaves per cutting among the treatments. The overall survival of rooted cuttings after transfer to polybag significantly (p<0.5) differed among the cuttings treated with different IBA solution. The highest survival percentage (69.67%) was observed in the cuttings rooted with 0.4% IBA treatment and the lowest (55.6%) survival was found in cuttings treated with control. The use of 0.4% IBA treatment is suggested for rooting of juvenile leafy branch cutting of S. macrophylla.

scholarly journals The prognostication of the rooting ability of apple stem cuttings by indices of seasonal growth of shoots

2021 ◽  
Vol 89-90 ◽  
pp. 101-109
Author(s):  
Alla Konopelko
Keyword(s):  
Callus Formation ◽  
Average Length ◽  
Total Duration ◽  
Biologically Active ◽  
Stem Cuttings ◽  
Seasonal Growth ◽  
Strong Negative Correlation ◽  
Rooted Cuttings ◽  
Rooting Ability ◽  
Short Period

The study involved seven species and cultivars of ornamental apple trees including Malus × floribunda, M. halliana, M. niedzwetzkyana, M. × purpurea, M. × purpurea ‘Ola’, M. × purpurea ‘Royalty’, and M. × purpurea ‘Selkirk’. The average value of increment, average length, and duration of shoots’ growth were determined to characterize the seasonal growth of Malus shoots. The percentages of rooted cuttings, callus formation without roots, and unviable cuttings were registered for each investigated taxon. Statistical analysis was performed in Microsoft Excel 2007 following Zaitsev (1990), and Atramentova & Utevskaya (2014).The total duration of shoots growth of the studied species and varieties ranged from 72 (M. × purpurea ‘Ola’) to 118 days (M. niedzwetzkyana); annual shoots reached a length from 213.75 mm (M. × purpurea) to 448.75 mm (M. niedzwetzkyana); the average increment of shoots ranged from 3.90 mm (M. × purpurea ‘Ola’) to 14.70 mm (M. floribunda).The rooting ability of Malus stem cuttings was limited to a reasonably short period of cutting procedure and depended on the application of biologically active substances, their concentrations, and complexes. The highest rooting rate (33.33 %) was observed in M. × purpurea ‘Ola’ after the treatment of its cuttings by 0.6 % indole-3-butyric acid (IBA) at the end of June. A slightly lower rooting rate (20.00 %) was observed in M. halliana cuttings treated by 0.4 % IBA and M. × purpurea ‘Selkirk’ cuttings treated by Podkorzen AB aqua at the end of June. Finally, 7.69 % of M. × floribunda cuttings were rooted with Podkorzen AB aqua at the beginning of July.The prediction of the rooting ability of Malus stem cuttings was confirmed by a strong negative correlation between the percentage of rooted cuttings and the duration of shoots’ growth (r = –0.88). Hence, the percentage of rooted cuttings increases with decreasing duration of shoots growth, depending on the genotype.

scholarly journals PENGARUH WAKTU PERENDAMAN STEK BATANG VANILI DALAM ZAT PENGATUR TUMBUH ROTOONE – F TERHADAP PERTUMBUHAN VANILI (VANILLA PLANIFOLIA ANDREWS)

AGRICA ◽  
2020 ◽  
Vol 3 (2) ◽  
pp. 86-98
Author(s):  
Philipus.N. Supardi ◽  
Sivester Seda
Keyword(s):  
Root Length ◽  
Immersion Time ◽  
Dry Weight ◽  
Root Diameter ◽  
Stem Cuttings ◽  
Vanilla Planifolia ◽  
Root Number ◽  
Soaking Time ◽  
Wet Weight ◽  
Number Of Shoots

This study aims to determine the effect of immersion time with the vanilla stem cuttings of plan growth regulators Rotoone – F of credit for the growth of roots and shoots of vanilla cuttings. The treatment used in this study is A0 (without immersion), A1 (immersion time 5 min), A2 (soaking time 10 min), and A3 (soaking time 15 min) variable observation in this study is the number of shoots, root number, number of The firs day of growing shoots, shoots length, root length, root diameter, wet weight and dry weight of cuttings of vanilla. The result showed that root growth best to treatment with the immersion time of 15 min (A3) is indicated by the highest number of roots, root length and diameter to treatment with the highest root of soaking time 15 min (A3) in 14 to 28 HST HST. The result showed that the growth of shoots best to treatment with the immersion time of 15 min (A3) is indicated by a number of days the first shoots to grow the fastest, most number of shoots, shoot length, wet weight and dry weight of the highest in treatments with soaking time 15 (A3) in 14 HST to 28 HST.

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