Analysis of seed protein diversity in Cicer arietinum L. genotypes with different seed coat colour using SDS-PAGE

SDS-PAGE technique was used for the study of seed protein polymorphism among three genotypes of Cicer arietinum with different seed coat colour. A total of 24 polypeptide bands were recorded. Out of these 20 were common among all three genotypes and 4 (16.66%) were polymorphic. The data analysis using UPGMA clustering revealed that genotypes with C2 (dark brown) and C3 (black) were closer as compared to genotype with C1 (light brown) coat colour. Jaccard similarity coefficient value ranged from 0.87 to 0.92. The similarity matrix was subjected to UPGMA clustering to generate dendrogram. The most closely revealed genotypes were C2 (dark brown) and C3 (black) with the highest similarity index 0.92 whereas, C1 (light brown) showed minimum similarity index with C3 (black) genotype 0.87. Each of three genotypes of C.arietinum had some polypeptide bands which were peculiar to them only. This enabled distinguishing all three genotypes on the basis of specific polypeptide fragments using SDS-PAGE analysis.

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Inter and Intra-specific Variation in SDS-PAGE Electrophoregrams of Total Seed Protein in Chickpea (Cicer arietinum L.) Germplasm

Pakistan Journal of Biological Sciences ◽

10.3923/pjbs.2003.1991.1995 ◽

2003 ◽

Vol 6 (24) ◽

pp. 1991-1995 ◽

Cited By ~ 3

Author(s):

Rehana Asghar ◽

Tayyaba Siddique ◽

Muhammad Afzal

Keyword(s):

Cicer Arietinum ◽

Seed Protein ◽

Cicer Arietinum L ◽

Sds Page ◽

Specific Variation ◽

Total Seed

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Correlation Studies of Some Agronomic Characters in Sesame

Experimental Agriculture ◽

10.1017/s0014479700005895 ◽

1970 ◽

Vol 6 (1) ◽

pp. 27-31 ◽

Cited By ~ 7

Author(s):

M. Osman Khidir ◽

H. El Gizouli Osman

Keyword(s):

Seed Size ◽

Seed Coat ◽

Coat Colour ◽

Stem Height ◽

Agronomic Characters ◽

Seed Coat Colour ◽

Correlation Studies ◽

First Maturity ◽

Criteria For Selection ◽

Number Of Branches

SummaryIn 90 local sesame types there was some association between seed coat colour and seed size, stem height, number of branches, number of pods, yield per plant and earliness. Forty-five coefficients show the degree of correlation between ten agronomic characters. Yield was significantly and positively correlated with all characters except the number of days to first flowering and to first maturity. Stem height, number of pods per plant and seed size seem to be the best criteria for selection in sesame.

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Internal recycling of respiratory CO2 in pods of chickpea (Cicer arietinum L.): the role of pod wall, seed coat, and embryo

Journal of Experimental Botany ◽

10.1093/jxb/erh190 ◽

2004 ◽

Vol 55 (403) ◽

pp. 1687-1696 ◽

Cited By ~ 46

Author(s):

R. T. Furbank

Keyword(s):

Cicer Arietinum ◽

Seed Coat ◽

Cicer Arietinum L ◽

Pod Wall

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Genotypic variation for phenolic compounds in developing and whole seeds, and storage conditions influence visual seed quality of yellow dry bean genotypes

Canadian Journal of Plant Science ◽

10.1139/cjps-2019-0153 ◽

2020 ◽

Vol 100 (3) ◽

pp. 284-295

Author(s):

Mei Xiong ◽

Mengli Zhao ◽

Zhen-Xiang Lu ◽

Parthiba Balasubramanian

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Seed Coat ◽

Storage Temperature ◽

Condensed Tannin ◽

Consumer Preference ◽

Coat Colour ◽

Seed Coat Colour ◽

Duration Of Storage

Seed coat colour is an important determinant of the visual quality of dry beans, as seeds are sold as a dry commodity. Phenolic compounds have a major effect on the colour of bean seeds. The objectives of the study were to determine the changes in phenolic compounds during seed development and in whole seeds of yellow bean genotypes with contrasting seed coat colour, and the effects of storage temperature and duration on seed phenolics and colour. Condensed tannin, phenolic acid, flavonoids, and antioxidant activity were observed as early as 10 d after flowering in the developing seeds of Arikara Yellow, which darken at harvest and during postharvest storage. In contrast, for CDC Sol and AAC Y073 seeds which remain yellow, phenolic compounds and antioxidant activity were consistently low. Seed brightness (L*) and yellow colour (b*) were negatively correlated with phenolic compounds and antioxidant activity, and conversely seed redness (a*) was positively correlated with phenolic compounds, confirming a negative influence of phenolic compounds on seed coat colour. Yellow bean genotypes had low anthocyanin but were high in β-carotene. Storage temperature influenced condensed tannin and seed coat colour, whereas the duration of storage influenced phenolic compounds, antioxidant activity, and seed coat colour. Higher temperatures (20 or 30 °C) and longer storage duration (120 or 180 d) generally resulted in darker seeds with increasing redness compared with seeds stored at 6 °C or for 60 d. AAC Y073 and CDC Sol with improved seed coat colour may increase consumer preference, value, and marketability of yellow beans.

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