scholarly journals Detection of Bovine TB infection in Pre-slaughter Cattle at Selected Abattoirs in Accra, Ghana Using the BOVIGAMTM 2G Assay

2020 ◽  
Vol 2 (10) ◽  
pp. 29-33
Author(s):  
Ivy Brago Amanor ◽  
Gloria Ivy Mensah ◽  
Raphael Amediko ◽  
James Edinam Futse ◽  
Kennedy Kwasi Addo
Keyword(s):  
Slaughter Cattle

scholarly journals Molecular detection of cattle Sarcocystis spp. in North-West Italy highlights their association with bovine eosinophilic myositis

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Selene Rubiola ◽  
Tiziana Civera ◽  
Felice Panebianco ◽  
Davide Vercellino ◽  
Francesco Chiesa
Keyword(s):  
18S Rdna ◽  
Inflammatory Myopathy ◽  
Molecular Techniques ◽  
Diaphragm Muscle ◽  
Economic Losses ◽  
Intermediate Hosts ◽  
Slaughter Cattle ◽  
Significant Difference ◽  
Pcr Targeting

Abstract Background Cattle are intermediate hosts of six Sarcocystis species, among which Sarcocystis hominis and Sarcocystis heydorni can infect humans through the consumption of raw or undercooked meat. In addition to the zoonotic potential, there is increasing interest in these protozoa because of the evidence supporting the role of Sarcocystis spp. in the occurrence of bovine eosinophilic myositis (BEM), a specific inflammatory myopathy which leads to carcass condemnation and considerable economic losses. Actually, all the prevalence studies carried out on cattle in Italy have been based on either morphological or 18S rDNA-based molecular techniques, most likely leading to misidentification of closely related species. Therefore, there is a strong need for new data on the prevalence of the different Sarcocystis spp. in cattle in Italy and their association with bovine eosinophilic myositis. Methods To reach our aim, individual striated muscle samples from BEM condemned carcasses (N = 54) and diaphragm muscle samples from randomly sampled carcasses (N = 59) were obtained from Northwest Italy slaughterhouses. Genomic DNA was extracted and analyzed by multiplex-PCR targeting 18S rDNA and cox1 genes. PCR products amplified using the genus-specific primer set in absence of the specific fragment for S. hirsuta, S. cruzi, S. hominis or S. bovifelis were sequenced to achieve species identification. Results Sarcocystis DNA was detected in 67.8% of the samples from slaughter cattle and in 90.7% of the samples from BEM condemned carcasses. S. cruzi was identified as the most prevalent species in slaughter cattle (61%), followed by S. bovifelis (10.2%), S. hominis (8.5%) and S. hirsuta (1.7%). Notably, among the different Sarcocystis spp. detected, the presence of S. bovifelis and S. hominis was significantly higher in samples isolated from BEM condemned carcasses (46.3% and 40.7% respectively), while there was no statistically significant difference between the presence of S. cruzi or S. hirsuta in BEM condemned carcasses (42.6% and 1.8%, respectively) and randomly sampled carcasses. Furthermore, DNA sequence analysis revealed the presence of a putative new species in two carcasses. Conclusions Our study contributes to updating the data on the prevalence of the different Sarcocystis spp. in cattle in Italy, highlighting the presence of three Sarcocystis spp., S. cruzi, S. hominis and S. bovifelis, in BEM lesions and allowing us to speculate on the possible role of S. hominis and S. bovifelis as the major sarcosporidian species involved in bovine eosinophilic myositis. Graphic Abstract

scholarly journals PRESENCE AND LEVELS OF CONCENTRATION OF POLYCYCLIC AROMATIC HYDROCARBONS (PAHS) IN SMOKED FISH, HIDES AND SKIN OF SLAUGHTER CATTLE AND GOATS IN AWKA URBAN, NIGERIA

2019 ◽  
pp. 14-17
Author(s):  
IKECHUKWU OFOMATA ◽  
LYNDA ONYINYE OBODOECHI ◽  
REGINALD IKECHUKWU OBIDIKE ◽  
JOHN ANAELOM NWANTA
Keyword(s):  
World Health ◽  
Fish Tissues ◽  
Anambra State ◽  
Smoked Fish ◽  
Permissible Level ◽  
Flame Ionization ◽  
Slaughter Cattle ◽  
Polycyclic Aromatic ◽  
The Mean ◽  
Health Organization

Objective: This study evaluated the presence and concentration of PAHs in singed and unsinged hides and skin of slaughter cattle and goats and smoked and non-smoked fish in Awka urban area of Anambra State, Nigeria. Methods: Sixty samples of singed and unsinged hides and skin for were collected from Kwata slaughterhouse in Awka town where fish tissues were also collected from fish smoking spots in Awka urban. The samples were examined for the presence PAHs such as: benzo[a]pyrene; benzo[a]anthracene; benzo[k]flouranthene and indeno[1-2-3cd]pyrene using gas chromatography equipped with flame ionization detector. Results: The result showed that the mean concentrations (µg/kg) of benzo[a]pyrene and indeno [1-2-3cd]pyrene in unsinged cattle hides were 7.89±7.77 and 2.25±0.97 respectively. The mean concentrations upon singeing were of 15.81±14.93 and 3.55±2.89. The mean concentrations (µg/kg) of benzo[a]pyrene; benzo[k]flouranthene and indeno[1-2-3cd]pyrene in unsigned goat skins were 6.52±10.77; 6.58±5.93 and 3.48±0.00 respectively. When the goatskin was singed, the mean concentrations were 17.50±26.03; 10.84±11.19 and 8.79±11.77. The mean concentrations (µg/kg) of benzo[a]pyrene; benzo[k]flouranthene and indeno[1-2-3cd]pyrene in non-smoked fish were 8.65±9.67; 2.11±2.10 and 54.76±60.35 respectively. When the fish were smoked, the mean concentrations of 26.42±40.21; 6.64±11.11 and 599.36±1395.61 were recorded respectively. In all species of animals sampled, no trace of benzo[a]anthracene was detected. Conclusion: The mean concentrations of all carcinogenic PAHs detected in this work were below the maximum permissible level set by World Health Organization (WHO) and European Commission (30µg/kg) with the exception of indeno[1-2-3cd]pyrene in smoked and non-smoked fish, which was far above the permissible level.

scholarly journals From unloading to trimming: studying bruising in individual slaughter cattle

2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Helen C Kline ◽  
Zachary D Weller ◽  
Temple Grandin ◽  
Ryan J Algino ◽  
Lily N Edwards-Callaway
Keyword(s):  
Confidence Interval ◽  
Sample Size ◽  
Animal Welfare ◽  
Economic Value ◽  
Traumatic Events ◽  
Visual Assessment ◽  
Slaughter Cattle ◽  
Number Of Factors ◽  
Trim Loss ◽  
Analyze Data

Abstract Livestock bruising is both an animal welfare concern and a detriment to the economic value of carcasses. Understanding the causes of bruising is challenging due to the numerous factors that have been shown to be related to bruise prevalence. While most cattle bruising studies collect and analyze data on truckload lots of cattle, this study followed a large number (n = 585) of individual animals from unloading through postmortem processing at five different slaughter plants. Both visual bruise presence and location was recorded postmortem prior to carcass trimming. By linking postmortem data to animal sex, breed, trailer compartment, and traumatic events at unloading, a rich analysis of a number of factors related to bruise prevalence was developed. Results showed varying levels of agreement with other published bruising studies, underscoring the complexity of assessing the factors that affect bruising. Bruising prevalence varied across different sex class types (P < 0.001); 36.5% of steers [95% confidence interval (CI): 31.7, 41.6; n = 378], 52.8% of cows (45.6, 60.0; 193), and 64.3% of bulls (no CI calculated due to sample size; 14) were bruised. There was a difference in bruise prevalence by trailer compartment (P = 0.035) in potbelly trailers, indicating that cattle transported in the top deck were less likely to be bruised (95% CI: 26.6, 40.4; n = 63) compared to cattle that were transported in the bottom deck (95% CI: 39.6, 54.2; n = 89). Results indicated that visual assessment of bruising underestimated carcass bruise trimming. While 42.6% of the carcasses were visibly bruised, 57.9% of carcasses were trimmed due to bruising, suggesting that visual assessment is not able to capture all of the carcass loss associated with bruising. Furthermore, bruises that appeared small visually were often indicators of larger, subsurface bruising, creating an “iceberg effect” of trim loss due to bruising.

scholarly journals Potential and competitiveness of EU countries in terms of slaughter livestock production

2019 ◽  
Vol 65 (No. 12) ◽  
pp. 550-559 ◽  
Author(s):  
Agnieszka Tłuczak
Keyword(s):  
European Union ◽  
Great Britain ◽  
Spatial Econometrics ◽  
Cattle Production ◽  
Strong Concentration ◽  
Eu Countries ◽  
Slaughter Cattle ◽  
European Union Countries ◽  
The Impact ◽  
The Relationship

Models and methods of spatial econometrics are gaining more and more popularity. Their advantage is the opportunity to examine the interrelationships between individual territorial units. These methods, apart from the own potential of the region, take into account the impact of neighbouring objects and location in space. The aim of the study is to examine the relationship between the potential and the level of competitiveness of individual European Union countries in the field of slaughter cattle production. In addition, the paper attempts to determine the specialisation of individual EU countries in the production of slaughter animals by sector. The analysis covered the years 2010–2016, using Eurostat data. The obtained results allow indicating countries in which there is a strong concentration of income potential (Sweden, Spain, Great Britain, France and Belgium). Countries in which the highest values of the potential quotients in the entire European Union are distinguished (Poland, Finland and Belgium).<br />

Prevalence and Antibiograms of Salmonellae in Slaughter Cattle, Slaughter Areas and Effluents in Zaria Abattoir, Nigeria

1989 ◽  
Vol 52 (4) ◽  
pp. 232-235 ◽  
Author(s):  
ABIODUN A. ADESIYUN ◽  
OYINDASOLA O. ONI
Keyword(s):  
Lymph Nodes ◽  
Antimicrobial Agents ◽  
Food Hygiene ◽  
Mesenteric Lymph Nodes ◽  
Widespread Occurrence ◽  
Slaughter Cattle ◽  
Mesenteric Lymph ◽  
High Incidence ◽  
Resistance Patterns ◽  
Health Significance

Mesenteric lymph nodes of slaughter cattle, swabs of slaughter and dressing areas and effluents of Zaria abattoir were cultured for salmonellae. Of a total of 510 samples cultured, 23 (5%) were positive for Salmonella. Five (4%) of 118 bovine lymph nodes contained salmonellae whereas sites swabbed during dressing had a frequency of isolation of 7% (11 of 150) and only 4 (3% of 150 samples) were positive after cleaning of dressing areas. Three (3%) of 92 effluent samples contained salmonellae. Thirteen of the 23 isolates of Salmonella were of different serotypes. The predominant serotypes were S. dublin (4 isolates), S. widemarsh (4 isolates) and S. Handoff (3 isolates). Twenty (87%), 8 (35%) and 8 (35%) isolates were resistant to streptomycin, neomycin and tetracycline respectively, while 6 (26%), 5 (22%) and 3(13%) isolates were not susceptible to gentamicin, ampicillin and chloramphenicol respectively. Overall, 15 resistance patterns were observed. The widespread occurrence of salmonellae in Zaria abattoir coupled with the high incidence of resistance to antimicrobial agents are of public health significance from the viewpoint of food hygiene and therapy for salmonellosis.

scholarly journals PRESENCE AND LEVELS OF CONCENTRATION OF POLYCYCLIC AROMATIC HYDROCARBONS (PAHS) IN SMOKED FISH, HIDES AND SKIN OF SLAUGHTER CATTLE AND GOATS IN AWKA URBAN, NIGERIA

2019 ◽  
pp. 14-17
Author(s):  
IKECHUKWU OFOMATA ◽  
LYNDA ONYINYE OBODOECHI ◽  
REGINALD IKECHUKWU OBIDIKE ◽  
JOHN ANAELOM NWANTA
Keyword(s):  
World Health ◽  
Fish Tissues ◽  
Anambra State ◽  
Smoked Fish ◽  
Permissible Level ◽  
Flame Ionization ◽  
Slaughter Cattle ◽  
Polycyclic Aromatic ◽  
The Mean ◽  
Health Organization

Objective: This study evaluated the presence and concentration of PAHs in singed and unsinged hides and skin of slaughter cattle and goats and smoked and non-smoked fish in Awka urban area of Anambra State, Nigeria. Methods: Sixty samples of singed and unsinged hides and skin for were collected from Kwata slaughterhouse in Awka town where fish tissues were also collected from fish smoking spots in Awka urban. The samples were examined for the presence PAHs such as: benzo[a]pyrene; benzo[a]anthracene; benzo[k]flouranthene and indeno[1-2-3cd]pyrene using gas chromatography equipped with flame ionization detector. Results: The result showed that the mean concentrations (µg/kg) of benzo[a]pyrene and indeno [1-2-3cd]pyrene in unsinged cattle hides were 7.89±7.77 and 2.25±0.97 respectively. The mean concentrations upon singeing were of 15.81±14.93 and 3.55±2.89. The mean concentrations (µg/kg) of benzo[a]pyrene; benzo[k]flouranthene and indeno[1-2-3cd]pyrene in unsigned goat skins were 6.52±10.77; 6.58±5.93 and 3.48±0.00 respectively. When the goatskin was singed, the mean concentrations were 17.50±26.03; 10.84±11.19 and 8.79±11.77. The mean concentrations (µg/kg) of benzo[a]pyrene; benzo[k]flouranthene and indeno[1-2-3cd]pyrene in non-smoked fish were 8.65±9.67; 2.11±2.10 and 54.76±60.35 respectively. When the fish were smoked, the mean concentrations of 26.42±40.21; 6.64±11.11 and 599.36±1395.61 were recorded respectively. In all species of animals sampled, no trace of benzo[a]anthracene was detected. Conclusion: The mean concentrations of all carcinogenic PAHs detected in this work were below the maximum permissible level set by World Health Organization (WHO) and European Commission (30µg/kg) with the exception of indeno[1-2-3cd]pyrene in smoked and non-smoked fish, which was far above the permissible level.

scholarly journals Distribution of Shiga-Toxigenic Escherichia coli O157 in the Gastrointestinal Tract of Naturally O157-Shedding Cattle at Necropsy

2010 ◽  
Vol 76 (15) ◽  
pp. 5278-5281 ◽  
Author(s):  
James E. Keen ◽  
William W. Laegreid ◽  
Carol G. Chitko-McKown ◽  
Lisa M. Durso ◽  
James L. Bono
Keyword(s):  
Escherichia Coli ◽  
Gastrointestinal Tract ◽  
Escherichia Coli O157 ◽  
Slaughter Cattle ◽  
Entire Gastrointestinal Tract

ABSTRACT Shiga-toxigenic Escherichia coli (STEC) O157 occurrence was determined along the entire gastrointestinal tract (GIT) of each of four naturally shedding cattle and at three sites in 61 slaughter cattle. STEC O157 was distributed along the entire GIT, though interanimal distribution was variable. Neither feces nor rectoanal-junction samples accurately predicted the STEC O157-negative status of any particular animal.

The effect of feed type on mouth lesions in slaughter cattle

1994 ◽  
Vol 74 (3) ◽  
pp. 571-573 ◽  
Author(s):  
D. B. Karren ◽  
L. A. Goonewardene ◽  
J. A. Bradley
Keyword(s):  
Lymph Node ◽  
Key Words ◽  
Slaughter Cattle ◽  
Lymph Node Enlargement ◽  
Key Words Incidence

The incidence of tongue lesions in slaughter cattle was 19.1%. Although the incidence of lymph node enlargement was low, it was five times higher in cattle with tongue lesions. Carcass weights were not affected by the presence of mouth lesions. The incidence of lesions was significantly higher (P < 0.00001) for cattle finished on silage from semi-dwarf rough awn (29.3%) compared to normal stem rough awn (13.5%) and normal stem smooth awn (11.8%) barley. Key words: Incidence, lesions, tongue, barley, cattle.

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