Phenotype analysis of unstimulated lymphocytes and anti-cd3-stimulated proliferating t-cells from cerebrospinal fluid and peripheral blood in patients with multiple sclerosis and other neurological diseases

Intracellular production of TNFα and IL-2 after stimulation with phorbol myristate/ionomycin was flowcytometrically measured in CD4+ T cells from peripheral blood (PB) and cerebrospinal fluid (CSF) of 29 patients with multiple sclerosis (MS), and 16 with other inflammatory and 41 with other non-inflammatory neurological diseases. In CSF, the percentages of CD4+TNFα+IL-2−T cells were significantly higher in patients with MS than either of the controls, whereas no difference was found in CD4+TNFα+IL-2+T or CD4+TNFα−IL-2+T cells. The increase was more pronounced at relapse than in remission. No significant change was detected in PB. These findings suggested that CD4+TNFα+IL-2−T cells are intrathecally upregulated in MS.

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Comparison of the response to phytohemagglutinin, of peripheral blood monuclear cells and cerebrospinal fluid lymphocytes in multiple sclerosis and other neurological diseases

Acta Neurologica Scandinavica ◽

10.1111/j.1600-0404.1986.tb04628.x ◽

1986 ◽

Vol 74 (1) ◽

pp. 66-71 ◽

Cited By ~ 1

Author(s):

E. Tournier-Lasserve ◽

E. Roullet ◽

O. Lyoncaen ◽

M.A. Bach

Keyword(s):

Multiple Sclerosis ◽

Cerebrospinal Fluid ◽

Peripheral Blood ◽

Neurological Diseases

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T cells from multiple sclerosis patients recognize immunoglobulin G from cerebrospinal fluid

Multiple Sclerosis Journal ◽

10.1191/1352458503ms906oa ◽

2003 ◽

Vol 9 (3) ◽

pp. 228-234 ◽

Cited By ~ 13

Author(s):

T Holmøy ◽

B Vandvik ◽

F Vartdal

Keyword(s):

Multiple Sclerosis ◽

T Cells ◽

Cerebrospinal Fluid ◽

T Cell ◽

Mononuclear Cells ◽

Neurological Diseases ◽

Affinity Maturation ◽

Peripheral Blood Mononuclear ◽

Number Of Patients ◽

Multiple Sclerosis Patients

Idiotopic sequences are created after V, D and J recombinations and by somatic mutations during affinity maturation of immuglobulin (Ig) molecules, and may therefore be potential immunogenic epitopes. Idiotope-specific T cells are able to activate and sustain the B cells producing such idiotopes. It is therefore possible that idiotope-specific intrathecal T cells could help maintain the persisting intrathecal synthesis of oligoclonal IgG observed in patients with multiple sclerosis (MS). This study was undertaken to examine T-cell responses to cerebrospinal fluid (CSF) IgG. Peripheral blood mononuclear cells (PBMC) from 14 of 21 MS patients and four of 17 control patients with other neurological diseases proliferated upon stimulation with autologous C SF IgG, while five and three, respectively, responded to serum IgG. By comparison, responses to myelin basic protein were recorded in only four MS and three control patients. Data from a limited number of patients indicate that the C SF IgG responsive cells were CD4+ and human leucocyte antigen DR restricted, that PBMC also respond to C SF IgG from other MS patients and that the C SF may contain T cells responding to autologous C SF IgG. This suggests that C SF IgG, or substances bound to this IgG, may represent T-cell immunogens, which could contribute to the intrathecal immune response in MS.

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Regulation of natural killer cell cytotoxicity by prostaglandin E in the peripheral blood and cerebrospinal fluid of patients with multiple sclerosis and other neurological diseases part 2. Effect of Exogenous PGE1 on spontaneous and interferon-induced natural killer☆

Journal of Neuroimmunology ◽

10.1016/0165-5728(83)90038-3 ◽

1983 ◽

Vol 4 (3) ◽

pp. 239-251 ◽

Cited By ~ 11

Author(s):

Jean E. Merrill ◽

Lawrence W. Myers ◽

George W. Ellison

Keyword(s):

Multiple Sclerosis ◽

Cerebrospinal Fluid ◽

Natural Killer Cell ◽

Natural Killer ◽

Peripheral Blood ◽

Neurological Diseases ◽

Killer Cell ◽

Prostaglandin E ◽

Cell Cytotoxicity ◽

Natural Killer Cell Cytotoxicity

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Quantification of EBNA-1-Specific CD8+ T-Cells in Cerebrospinal Fluid and Peripheral Blood of Patients with Multiple Sclerosis (P02.076)

Neurology ◽

10.1212/wnl.78.1_meetingabstracts.p02.076 ◽

2012 ◽

Vol 78 (Meeting Abstracts 1) ◽

pp. P02.076-P02.076

Author(s):

H. Erdur ◽

C. Meisel ◽

C. Schonemann ◽

K.-P. Wandinger ◽

B. Rosche

Keyword(s):

Multiple Sclerosis ◽

T Cells ◽

Cerebrospinal Fluid ◽

Cd8 T Cells ◽

Peripheral Blood

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Increased Intrathecal Chemokine Receptor CCR2 Expression in Multiple Sclerosis

Biomarker Insights ◽

10.1177/117727190700200028 ◽

2007 ◽

Vol 2 ◽

pp. 117727190700200 ◽

Cited By ~ 5

Author(s):

Hideto Nakajima ◽

Masakazu Sugino ◽

Fumiharu Kimura ◽

Toshiaki Hanafusa ◽

Toshiyuki Ikemoto ◽

...

Keyword(s):

Multiple Sclerosis ◽

Flow Cytometry ◽

T Cells ◽

Cerebrospinal Fluid ◽

Chemokine Receptor ◽

Neurological Diseases ◽

Healthy Controls ◽

Color Flow ◽

Ccr2 Expression ◽

Chemokine Receptor Ccr2

Expression of CCR2, CXCR3 and CCR4 on CD4+ T or CD8+ T cells in blood and cerebrospinal fluid (CSF) for multiple sclerosis (MS) was measured by 3-color flow cytometry, and compared to blood from healthy controls and CSF from patients with other inflammatory neurological diseases (INDs). CD4+CXCR3+/CD4+CCR4+ ratio (representing Th1/Th2 balance) was higher in both CSF and blood of MS patients than those of IND patients or healthy controls. Percentage of CCR2-positive T cells was significantly higher in CSF from MS patients. Increased CCR2 expression on T cells in CSF and Th1/Th2 imbalance may reflect the pathological processes involved in MS.

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