Effect of diazepam on serum testosterone and the ventral prostate gland in male rats

1979 ◽  
Vol 3 (1) ◽  
pp. 31-35 ◽  
Author(s):  
P. S. Cook ◽  
M. Notelovitz ◽  
P. S. Kalra ◽  
S. P. Kalra
Keyword(s):  
Prostate Gland ◽  
Serum Testosterone ◽  
Male Rats ◽  
Ventral Prostate

HYPOTHALAMIC-PITUITARY-TESTICULAR SYSTEM FOLLOWING TESTICULAR X-IRRADIATION

1976 ◽  
Vol 83 (1) ◽  
pp. 190-200 ◽  
Author(s):  
H. L. Verjans ◽  
K. B. Eik-Nes
Keyword(s):  
Interstitial Cell ◽  
Serum Testosterone ◽  
Male Rats ◽  
Ventral Prostate ◽  
Testis Weight ◽  
X Ray ◽  
Serum Lh ◽  
Testicular Weight ◽  
Unknown Factor ◽  
X Irradiation

ABSTRACT Testes of adult, male rats were exposed to a total dose of 1500 R of X-irradiation. Testicular weight decreased from day 8 after X-ray treatment. This decrease was, however, preceded by an increment of the testis weight on day 4 following treatment. X-ray treatment of testes was associated with significant increases in serum FSH. Testicular irradiation had, however, no effect on ventral prostate and seminal vesicles weights. Serum testosterone increased only on day 1, 2 and 4 after irradiation, while serum LH levels tended to increase from day 8 post-irradiation. These changes were not significant, however, when compared with non-irradiated controls. At 7, 13 and 20 days following 1500 R of bilateral, testicular X-irradiation, the hypothalamic-pituitary unit was still capable of responding to exogenous gonadotrophin releasing factor. Serum FSH may in male rats be regulated at least partly by circulating steroids of testicular origin and partly by an unknown factor of non-interstitial cell nature.

ANDROGEN RECEPTORS IN THE RAT BRAIN, ANTERIOR PITUITARY GLAND AND VENTRAL PROSTATE GLAND: EFFECTS OF ORCHIDECTOMY AND AGEING

1979 ◽  
Vol 81 (1) ◽  
pp. 75-81 ◽  
Author(s):  
B. D. GREENSTEIN
Keyword(s):  
Pituitary Gland ◽  
Binding Sites ◽  
Anterior Pituitary ◽  
Androgen Receptors ◽  
Prostate Gland ◽  
Anterior Pituitary Gland ◽  
Male Rats ◽  
Ventral Prostate ◽  
Binding Reaction ◽  
Old Rats

Available high-affinity binding sites for 5α-dihydrotestosterone (DHT) were measured in cytosols obtained from the amygdala, hypothalamus, anterior pituitary gland and ventral prostate gland of 12-week-old rats at various times after orchidectomy, and in the corresponding tissues of 18-month-old male rats. It is suggested that the lower affinity of the DHT binding reaction in brain and ventral prostatic cytosols after orchidectomy or ageing respectively, may explain, at least in part, the changes in the responsiveness of the tissues to androgens.

Effects on developmental landmarks and reproductive capability of 3,3',4,4'-Tetrachlorobiphenyl and 3,3',4,4',5- Pentacholorobiphenyl in offspring of rats exposed during pregnancy

1998 ◽  
Vol 17 (7) ◽  
pp. 365-372 ◽  
Author(s):  
A S Faqi ◽  
P R Dalsenter ◽  
H-J Merker ◽  
I Chahoud
Keyword(s):  
Serum Testosterone ◽  
In Utero ◽  
Male Offspring ◽  
Male Rats ◽  
Ventral Prostate ◽  
In Utero Exposure ◽  
Neonatal Hypothyroidism ◽  
Reproductive Effects ◽  
Pcb 126 ◽  
Daily Sperm Production

1 Pregnant Wistar rats were treated orally with a single dose of 100 mg3,3',4,4'-tetrachlorobiphenyl (PCB 77)/ kg b.w. or 10 mg3,3',4,4',5 pentachlorobiphenyl (PCB 126)/kg b.w. on day 15 of pregnancy. The control rats received peanut oil at the same day. Developmental landmarks were assessed in all offspring rats and reproductive effects of PCB 77 and PCB 126 on male offspring were studied on postnatal day 65 (at puberty) and on postnatal day 140 (at adulthood). 2 The ano-genital distance as well as the ratio anogenital distance to body length was reduced in male pups of the PCB 126 group and the age at vaginal opening was significantly delayed in the female pups. 3 Testis, brain weights and daily sperm production were permanently increased and seminal vesicle weights were decreased in male offspring of the PCB 77 group. In male rats of PCB 126 group, the brain weights were permanently increased and ventral prostate weights permanently reduced. In both PCB groups, however, serum testosterone concentration was reduced only at adulthood. Additionally, the male rats of the PCB 126 group showed alterations in sexual behavior. In these rats the number of mounts with intromissions was significantly increased. 4 The results of this study show that PCB 126 elicits some TCDD-like reproductive effects after in utero exposure, while the reproductive effects of in utero exposure to PCB 77 on male offspring may be attributed to the neonatal hypothyroidism induced by the substance during early fetal development. Further studies using multiple doses and providing thyroid hormone data will be necessary to support this hypothesis.

scholarly journals Sleep Deprivation Alters Rat Ventral Prostate Morphology, Leading to Glandular Atrophy: A Microscopic Study Contrasted with the Hormonal Assays

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Daniel P. Venâncio ◽  
Monica L. Andersen ◽  
Patricia S. L. Vilamaior ◽  
Fernanda C. Santos ◽  
Adriano Zager ◽  
...  
Keyword(s):  
Sleep Deprivation ◽  
Paradoxical Sleep ◽  
Microscopic Analysis ◽  
Serum Testosterone ◽  
Male Rats ◽  
Ventral Prostate ◽  
Control Group ◽  
Morphological Alterations ◽  
Rat Ventral Prostate ◽  
Glandular Atrophy

We investigated the effect of 96 h paradoxical sleep deprivation (PSD) and 21-day sleep restriction (SR) on prostate morphology using stereological assays in male rats. After euthanasia, the rat ventral prostate was removed, weighed, and prepared for conventional light microscopy. Microscopic analysis of the prostate reveals that morphology of this gland was altered after 96 h of PSD and 21 days of SR, with the most important alterations occurring in the epithelium and stroma in the course of both procedures compared with the control group. Both 96 h PSD and 21-day SR rats showed lower serum testosterone and higher corticosterone levels than control rats. The significance of our result referring to the sleep deprivation was responsible for deep morphological alterations in ventral prostate tissue, like to castration microscopic modifications. This result is due to the marked alterations in hormonal status caused by PSD and SR.

INHIBITION OF STEROID 5α-REDUCTASE IN VIVO: EFFECT ON SUPPRESSION OF LUTEINIZING HORMONE AND STIMULATION OF ACCESSORY SEX ORGANS BY TESTOSTERONE IN THE ORCHIDECTOMIZED RAT

1979 ◽  
Vol 81 (2) ◽  
pp. 209-220 ◽  
Author(s):  
LIDIA WEI LIU KAO ◽  
JUDITH WEISZ
Keyword(s):  
Prostate Gland ◽  
Male Rats ◽  
Ventral Prostate ◽  
Promoting Effect ◽  
Accessory Sex Organs ◽  
Pituitary Glands ◽  
Lh Secretion ◽  
Carboxylic Acid Derivative

An inhibitor of 5α-reductase, the 17β-carboxylic acid derivative of testosterone (testosterone-17βCA), has been used to evaluate the importance of the 5α-reduction of testosterone in its action on the suppression of LH secretion in male rats. The potential of testosterone-17βCA to inhibit the formation of 5α-dihydrotestosterone (DHT) was first demonstrated in vitro. When homogenates of hypothalami or anterior pituitary glands were incubated with [3H]testosterone in the presence of a 50-fold excess of testosterone-17βCA, the formation of labelled DHT was inhibited by more than 80%. Adult male rats that had been castrated for 1–2 months were fitted with chronic intravenous catheters and implanted with silicone elastomer sheets: one group received one sheet, 0·5–2·0 cm2 in size containing 1·6% testosterone, a second group received one 50 cm2 sheet containing 1·6% testosterone-17βCA and a third group received two sheets, one sheet 50 cm2 in size containing 1·6% testosterone-17βCA and the second ranging in size from 0·5 to 2·0 cm2 and containing 1·6% testosterone. Blood was withdrawn daily from each rat over a 4–5 day period after implantation of the steroids and the level of LH in the plasma was measured by radioimmunoassay. The seminal vesicles and the ventral prostate gland were removed at autopsy on day 4 or 5; the weights of these organs were shown to have increased progressively as the size of the implant of testosterone increased. In contrast, the level of LH in the plasma was suppressed to a comparable extent by implants of testosterone between 0·6 and 2 cm2, whereas a 0·5 cm2 implant of testosterone had no effect. Implants of testosterone-17βCA alone did not influence the weight of the accessory organs or the level of LH. When testosterone-17βCA and testosterone were implanted together, the growth-promoting effect of the latter on the accessory sex organs was significantly reduced. The effectiveness of testosterone in suppressing the level of LH in the plasma of these animals was not influenced by the presence of testosterone-17βCA and in certain instances the level was raised.

Steroidal interactions in the ageing endocrine system: absence of suppression and pathology in reproductive systems of old males from a mixed-sex socially stressful rat colony

1993 ◽  
Vol 137 (1) ◽  
pp. 115-122 ◽  
Author(s):  
G. T. Taylor ◽  
M. Bardgett ◽  
S. Farr ◽  
S. Womack ◽  
D. Komitowski ◽  
...  
Keyword(s):  
Hpa Axis ◽  
Stress Responses ◽  
Reproductive System ◽  
Sperm Count ◽  
Prostate Gland ◽  
Male Rats ◽  
Ventral Prostate ◽  
Same Sex ◽  
Stable Conditions ◽  
Hpt Axis

ABSTRACT A paradigm using chronic social stress and multiple measures of the reproductive system were used to assess changes with ageing in the dynamics of endogenous steroid interactions. The 22- to 24-month-old male rats lived for 8 weeks in one of four types of colony, in groups of the same sex or groups of mixed sex including familiar or unfamiliar old males. Measures of endocrinology (circulating steroid levels), behaviour (exploration and sociosexual responses), physiology (body and organ weights and epididymal sperm count) and histology (adrenal and ventral prostate glands) served as markers of activation of the hypothalamic-pituitary-adrenal (HPA) or hypothalamic-pituitary-testicular (HPT) axes. Old males living under stable conditions as familiar same-sex colonies served as the comparison group. Results indicated clear chronic activation of the HPA axis in the unfamiliar all-male colonies and of the HPT axis in the familiar males from mixed-sex colonies, whereas both steroidal axes were stimulated in colonies of unfamiliar males and females. Findings from aged males under chronic stress suggested that reproductive dysfunction may be limited to situations in which activation of the HPA axis occurs without concurrent stimulation of the HPT axis. Data on steroidal interactions from mixed-sex groups suggested that (1) chronic excitation of the HPA failed to suppress function in the reproductive system of the old males, (2) their stress responses were little affected by chronic HPT activation and (3) there was no evidence for stress-induced pathology, even in the vulnerable prostate gland. The conclusion is that increased risks for urogenital pathology with long-term exposure to stress is not an inevitable outcome for ageing male rats nor, perhaps, for other social species living under conditions in which multiple endocrine systems typically undergo simultaneous activation. Journal of Endocrinology (1993) 137, 115–122

ANTIANDROGENIC EFFECTS OF THE PINEAL GLAND AND MELATONIN IN CASTRATED AND INTACT PREPUBERTAL MALE RATS

1978 ◽  
Vol 79 (1) ◽  
pp. 77-83 ◽  
Author(s):  
R. ALONSO ◽  
L. PRIETO ◽  
C. HERNANDEZ ◽  
M. MAS
Keyword(s):  
Pineal Gland ◽  
Prostate Gland ◽  
Antagonistic Effect ◽  
Seminal Vesicles ◽  
Male Rats ◽  
Ventral Prostate ◽  
Inhibitory Influence ◽  
High Concentration ◽  
Accessory Sex Organs ◽  
Prepubertal Rats

In castrated prepubertal rats, pinealectomy enhanced the testosterone-induced growth response of the seminal vesicles and melatonin inhibited this effect in a dose-related manner. In entire animals, the serum concentration of LH was increased after pinealectomy with no significant changes in other parameters. Administration of melatonin to intact, pinealectomized rats did not affect the serum concentrations of LH or testosterone but caused a doserelated decrease in the weight of the seminal vesicles. The highest dose of melatonin tested reduced the weight of the ventral prostate gland and the uptake of radioactivity by both the ventral prostate gland and the testes after injection of [5-3H]uridine. It is suggested that the pineal gland and melatonin may exert an antagonistic effect on the biological activity of androgens administered to castrated rats and that melatonin can reduce the growth of the accessory sex organs of intact, pinealectomized rats, in spite of a high concentration of LH in the serum. The well-known inhibitory influence of systemically administered melatonin on the accessory sex organs in male rats may be due to its antagonistic effect at a peripheral level.

METABOLISM AND MODE OF ACTION OF ANDROGENS IN TARGET TISSUES OF MALE RATS

1972 ◽  
Vol 69 (1) ◽  
pp. 165-173 ◽  
Author(s):  
H. Schmidt ◽  
I. Noack ◽  
K. D. Voigt
Keyword(s):  
Cell Proliferation ◽  
Cell Metabolism ◽  
Seminal Vesicles ◽  
Male Rats ◽  
Ventral Prostate ◽  
Nucleic Acid Content ◽  
The Difference ◽  
Independent Effects ◽  
Sites Of Action ◽  
Peripheral Metabolism

ABSTRACT The effect of testosterone and 5α-dihydrotestosterone on protein and nucleic acid content as well as on the activities of some enzymes has been studied in the ventral prostate and the seminal vesicles of immature castrated rats. Both androgens were given intraperitoneally in doses of 1 mg daily for one or three days the rats were sacrificed one day after the last injection. In the prostate it was found that 5α-dihydrotestosterone had a greater effect on DNA increase, i. e. cell proliferation than testosterone, whereas cell metabolism was stimulated by the two androgens to nearly the same extent. In the seminal vesicles a single dose led to the same results as had been obtained in the prostate, i. e. a greater cell proliferative action of 5α-dihydrotestosterone and an equal stimulation of cell metabolism by testosterone and 5α-dihydrotestosterone was also observed. When three doses of the two androgens were given, cell proliferation as well as cell metabolism in the seminal vesicles were significantly more increased after 5α-dihydrotestosterone than after testosterone. The difference of action after systemic administration of the two androgens is explained by their different accumulation and by their different peripheral metabolism in the target tissues. From the partly independent effects of various androgens on cell proliferation and cell metabolism the conclusion may be drawn that there exist at least two intracellular sites of action.

Protective effect of green tea extract against cadmium-induced testicular damage in rats in respect of oxidant/antioxidant equilibrium and androgen production

2020 ◽  
Vol 21 (1) ◽  
pp. 31-35
Author(s):  
Basma El-Desoky ◽  
Shaimaa El-Sayed ◽  
El-Said El-Said
Keyword(s):  
Gene Expression ◽  
Single Dose ◽  
Green Tea ◽  
Serum Testosterone ◽  
Green Tea Extract ◽  
Male Rats ◽  
Controlled Study ◽  
Control Group ◽  
Testicular Damage ◽  
Tea Extract

Objective: Investigating the effect of green tea extract (GTE) on the testicular damage induced by cadmium chloride CdCl2 in male rats. Design: Randomized controlled study. Animals: 40 male Wistar rats. Procedures: Rats were randomly divided into four groups: A) control group (each rat daily received pellet diet); B) GTE group each rat daily received pellet diet as well as 3 ml of 1.5 % w/v GTE, C) CdCl2 group each rat was I/P injected a single dose of 1 mg/kg CdCl2, then daily received pellet diet, and D) CdCl2+GTE group each rat was I/P injected a single dose of 1 mg/kg CdCl2 then daily received pellet diet as well as 3 ml of 1.5 % w/v GTE. After 30 days, blood samples were collected for hormonal assays (testosterone, FSH, and LH). In addition, both testes were collected; one of them was used for quantification of 17-beta hydroxysteroid dehydrogenase III (17β-HSDIII) gene expression using a real-time PCR. The other testis was used for determination of catalase and reduced glutathione; GSH, Nitric oxide (NO) and malondialdehyde (MDA) levels. Results: CdCl2 decreased serum testosterone levels and its synthesis pathway (17β-HSDIII testicular gene expression). While antioxidants catalase and GSH were reduced, oxidants MDA were enriched in the testes of CdCl2-poisoned rats. This CdCl2-promoted testicular dysfunction was corrected via the administration of GTE to male rats. Conclusion and clinical relevance: GTE could be used as a remedy for protecting against CdCl2-induced testicular damage in male rats.

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