Dependence of Lipase Activity on Water Content and Enzyme Concentration in Reverse Micelles

Basically, gelatin is pure protein food ingredient, obtained from thermal collagen denaturation from animals. Gelatin is used as a stabilizer, gelling agent, binder, thickener, emulsifier, adhesive, whipping agent, and food wrap which is edible coating. Protein levels in fish skin determine the amount of collagen contained in skin tissue, so the baronang fish skin has a considerable opportunity as a source of collagen to be hydrolyzed into gelatin. This study aimed to determine the characteristics of gelatin produced from baronang fish skin by enzymatic extraction and to determine the best treatment of a combination of enzyme concentrations with extraction time. Making gelatine was carried out by hydrolysis using bromelin enzyme at a concentration of 1%, 1.5% and 2% with extraction time of 2 hours, 4 hours and 6 hours. The result, obtained gelatine with water content ranging from 3.13-5.83%, the water content ranged from 0.17-3.56%, ash content 0.35 - 3.65% and protein levels ranged from 91-94, 72%. The chemical characteristics of gelatine from baronang fish skin have a yield of up to 6%, water content <6%, ash content <4% and protein content reaching 94%. The best treatment was obtained at 1% enzyme concentration and extraction for 4 hours with a protein content of 94.72%.

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Solute dynamics in block-copolymer reverse micelles: Do water content and copolymer concentration alter the microenvironment?

The Journal of Chemical Physics ◽

10.1063/1.4882905 ◽

2014 ◽

Vol 140 (23) ◽

pp. 234905 ◽

Cited By ~ 2

Author(s):

Sugosh R. Prabhu ◽

G. B. Dutt

Keyword(s):

Block Copolymer ◽

Water Content ◽

Reverse Micelles ◽

Copolymer Concentration ◽

Solute Dynamics

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On the Origin of Unchanged Lipase Activity Profile in Cationic Reverse Micelles†

Langmuir ◽

10.1021/la990366t ◽

2000 ◽

Vol 16 (1) ◽

pp. 76-80 ◽

Cited By ~ 23

Author(s):

Prasanta Kumar Das ◽

Arabinda Chaudhuri

Keyword(s):

Lipase Activity ◽

Reverse Micelles ◽

Activity Profile

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STUDI PEMBUATAN MINYAK KELAPA MURNI SECARA ENZIMATIS DENGAN MENGGUNAKAN BERBAGAI TINGKAT KONSENTRASI ENZIM NANAS PADA DUA JENIS KELAPA

Jurnal Agroqua Media Informasi Agronomi dan Budidaya Perairan ◽

10.32663/ja.v11i1.42 ◽

2017 ◽

Vol 11 (1) ◽

pp. 24

Author(s):

Prihanani Prihanani ◽

Danner Sagala ◽

Yondi Yonadi

Keyword(s):

Water Content ◽

Coconut Oil ◽

Enzyme Concentration ◽

Market Quality ◽

Virgin Coconut Oil ◽

Randomized Design ◽

Completely Randomized Design

Virgin coconut oil can be proccessed in many ways to meet market quality. One of them is enzymaticly process. The aim of this research was to know bromelin enzyme concentration on two varieties of coconut. The experiment was conducted at PT. Sandabi Indah Lestari laboratory, Lunjuk Village, Seluma Barat Subistrict and Seluma Regency from in July 2012. The experiment was arranged in completely randomized design with two factos and three replication. The first faktor was variety of coconut consisted of Kelapa dalam and Kelapa hibrida. The second factor was bromelin enzyme concentration consisted of LIPI enzyme (as control), 50, 100, 150 gram. Variety affected most significantly on virgin coconut oil rendement. Kelapa dalam variety produced the higher virgin coconut oil than kelapa hibrida variety. However, variety and enzyme were affected unsignificant on water content of the virgin coconut oil.

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Determination the Optimum Conditions of the Activity and Stability of Lipase Extracted from Sunflower Germinated Seeds

Iraqi Journal of Science ◽

10.24996/ijs.2021.62.2.8 ◽

2021 ◽

pp. 431-440

Author(s):

Alaa M. Dh. Al-Haidari ◽

Ibrahim S. Alsaadawi ◽

Saad Hussein Khudhair

Keyword(s):

Lipase Activity ◽

Specific Activity ◽

Enzyme Reaction ◽

Total Activity ◽

Enzyme Concentration ◽

Sunflower Seeds ◽

Optimum Conditions ◽

Inhibitory Effects ◽

Mineral Salts ◽

Lipase Enzyme

The present study was conducted to determine the optimum conditions required for lipase enzyme activity extracted from germinated sunflower seeds, including temperature, pH, agitation, time of incubation, enzyme concentration, substrate type, and concentrations of mineral salts and EDTA. Optimum pH, temperature and time of incubation required for lipase stability were also determined. The results showede optimum lipase activity (3.251U/ml) wasund at 30 ̊C and pH 7 after 20 minutes of incubation when using 1 ml lipase enzyme with 0.02 ml of CaCl2 (10 mM) at 100 rpm of agitation and in the presence of olive oil as the substrate for enzyme reaction. EDTA appeared to have inhibitory effects, while Ca+2 and Mg+2 have stimulatory effects on lipase activity. The values of lipase activity, total activity, and specific activity measured under optimum conditions were increased by 36.99%, 36.95%, and 38.21% over control, respectively. The enzyme showed stability at a temperature ranged between 30 to 50 ˚C, pH between 7 to 8, and time of incubation between 10 to 40 minutes. These results suggest that lipase enzyme extracted from germinated sunflower seeds have stability that depends on pH, temperature, and incubation period, which enables it to be used in different industries.

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