High-throughput fluorescence microscopic analysis of protein abundance and localization in budding yeast

2016 ◽  
Vol 51 (2) ◽  
pp. 110-119 ◽  
Author(s):  
Nikko P. Torres ◽  
Brandon Ho ◽  
Grant W. Brown
Keyword(s):  
High Throughput ◽  
Budding Yeast ◽  
Microscopic Analysis ◽  
Protein Abundance ◽  
Fluorescence Microscopic Analysis

scholarly journals Quantification of enterohemorrhagic Escherichia coli O157:H7 protein abundance by high-throughput proteome

PLoS ONE ◽  
2018 ◽  
Vol 13 (12) ◽  
pp. e0208520 ◽  
Author(s):  
Wanderson Marques Da Silva ◽  
Jinlong Bei ◽  
Natalia Amigo ◽  
María Pía Valacco ◽  
Ariel Amadio ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
High Throughput ◽  
Enterohemorrhagic Escherichia Coli ◽  
Escherichia Coli O157 ◽  
Protein Abundance

In Vitro Anti-Biofilm Activity of Hydrogen-Peroxide Generating Electrochemical Bandage Against Yeast Biofilms

2021 ◽  
Author(s):  
Yash S. Raval ◽  
Abdelrhman Mohamed ◽  
Jayawant N. Mandrekar ◽  
Cody Fisher ◽  
Kerryl E. Greenwood-Quaintance ◽  
...  
Keyword(s):  
Membrane Damage ◽  
Microscopic Analysis ◽  
Viable Cell ◽  
Wound Infections ◽  
Unique Challenge ◽  
Cell Membrane Damage ◽  
Cell Counts ◽  
Extensive Cell ◽  
Fluorescence Microscopic Analysis

Wound infections are caused by bacteria and/or fungi. The presence of fungal biofilms in wound beds presents a unique challenge, as fungal biofilms may be difficult to eradicate. The goal of this work was to assess the in vitro anti-biofilm activity of a H 2 O 2 -producing electrochemical bandage (e-bandage) against 15 yeast isolates representing commonly-encountered species. Time-dependent decreases in viable biofilm CFU counts of all isolates tested were observed, resulting in no visible colonies with 48 hours of exposure by plate culture. Fluorescence microscopic analysis showed extensive cell membrane damage of biofilm cells after e-bandage treatment. Reductions in intracellular ATP levels of yeast biofilm cells were recorded post e-bandage treatment. Our results suggest that exposure to H 2 O 2 -producing e-bandages reduce in vitro viable cell counts of yeast biofilms, making this a potential new topical treatment approach for fungal wound infections.

scholarly journals High Throughput Screening for Compounds That Alter Muscle Cell Glycosylation Identifies New Role forN-Glycans in Regulating Sarcolemmal Protein Abundance and Laminin Binding

2012 ◽  
Vol 287 (27) ◽  
pp. 22759-22770 ◽  
Author(s):  
Paula V. Cabrera ◽  
Mabel Pang ◽  
Jamie L. Marshall ◽  
Raymond Kung ◽  
Stanley F. Nelson ◽  
...  
Keyword(s):  
Muscle Cell ◽  
High Throughput ◽  
High Throughput Screening ◽  
Protein Abundance ◽  
Laminin Binding

An X-ray fluorescence microscopic analysis of the tissue surrounding the multi-channel cochlear implant electrode array

2016 ◽  
Vol 17 (3) ◽  
pp. 129-131 ◽  
Author(s):  
Kathryn Spiers ◽  
Tina Cardamone ◽  
John B. Furness ◽  
Jonathan C. M. Clark ◽  
James F. Patrick ◽  
...  
Keyword(s):  
Cochlear Implant ◽  
Electrode Array ◽  
Microscopic Analysis ◽  
X Ray ◽  
Fluorescence Microscopic Analysis

scholarly journals High Throughput Analyses of Budding Yeast ARSs Reveal New DNA Elements Capable of Conferring Centromere-Independent Plasmid Propagation

2016 ◽  
Vol 6 (4) ◽  
pp. 993-1012 ◽  
Author(s):  
Timothy Hoggard ◽  
Ivan Liachko ◽  
Cassaundra Burt ◽  
Troy Meikle ◽  
Katherine Jiang ◽  
...  
Keyword(s):  
High Throughput ◽  
Budding Yeast ◽  
Dna Elements

Production of NAADP and its role in Ca2+ mobilization associated with lysosomes in coronary arterial myocytes

2006 ◽  
Vol 291 (1) ◽  
pp. H274-H282 ◽  
Author(s):  
Fan Zhang ◽  
Guo Zhang ◽  
Andrew Y. Zhang ◽  
Matthew J. Koeberl ◽  
Eryn Wallander ◽  
...  
Keyword(s):  
Coronary Arteries ◽  
Regulatory Mechanism ◽  
Microscopic Analysis ◽  
Bafilomycin A1 ◽  
Two Phase ◽  
Functional Studies ◽  
Adp Ribosyl Cyclase ◽  
Intracellular Ca ◽  
Arterial Smooth Muscle Cells ◽  
Fluorescence Microscopic Analysis

The present study was designed to determine the production of nicotinic acid adenine dinucleotide phosphate (NAADP) and its role associated with lysosomes in mediating endothelin-1 (ET-1)-induced vasoconstriction in coronary arteries. HPLC assay showed that NAADP was produced in coronary arterial smooth muscle cells (CASMCs) via endogenous ADP-ribosyl cyclase. Fluorescence microscopic analysis of intracellular Ca2+ concentration ([Ca2+]i) in CASMCs revealed that exogenous 100 nM NAADP increased [Ca2+]i by 711 ± 47 nM. Lipid bilayer experiments, however, demonstrated that NAADP did not directly activate ryanodine (Rya) receptor Ca2+ release channels on the sarcoplasmic reticulum. In CASMCs pretreated with 100 nM bafilomycin A1 (Baf), an inhibitor of lysosomal Ca2+ release and vacuolar proton pump function, NAADP-induced [Ca2+]i increase was significantly abolished. Moreover, ET-1 significantly increased NAADP formation in CASMCs and resulted in the rise of [Ca2+]i in these cells with a large increase in global Ca2+ level of 1,815 ± 84 nM. Interestingly, before this large Ca2+ increase, a small Ca2+ spike with an increase in [Ca2+]i of 529 ± 32 nM was observed. In the presence of Baf (100 nM), this ET-1-induced two-phase [Ca2+]i response was completely abolished, whereas Rya (50 μM) only markedly blocked the ET-1-induced large global Ca2+ increase. Functional studies showed that 100 nM Baf significantly attenuated ET-1-induced maximal constriction from 82.26 ± 4.42% to 51.80 ± 4.36%. Our results suggest that a lysosome-mediated Ca2+ regulatory mechanism via NAADP contributes to ET-1-induced Ca2+ mobilization in CASMCs and consequent vasoconstriction of coronary arteries.

scholarly journals Mcp4, a Meiotic Coiled-Coil Protein, Plays a Role in F-Actin Positioning during Schizosaccharomyces pombe Meiosis

2007 ◽  
Vol 6 (6) ◽  
pp. 971-983 ◽  
Author(s):  
Ayami Ohtaka ◽  
Daisuke Okuzaki ◽  
Takamune T. Saito ◽  
Hiroshi Nojima
Keyword(s):  
Subcellular Localization ◽  
Schizosaccharomyces Pombe ◽  
Coiled Coil ◽  
Microscopic Analysis ◽  
Specific Protein ◽  
Membrane Formation ◽  
Meiotic Progression ◽  
Specific Proteins ◽  
Fluorescence Microscopic Analysis

ABSTRACT Some meiosis-specific proteins of Schizosaccharomyces pombe harbor coiled-coil motifs and play essential roles in meiotic progression. Here we describe Mcp4, a novel meiosis-specific protein whose expression is abruptly induced at the horsetail phase and which remains expressed until sporulation is finished. Fluorescence microscopic analysis revealed that Mcp4 alters its subcellular localization during meiosis in a manner that partially resembles the movement of F-actin during meiosis. Mcp4 and F-actin never colocalize; rather, they are located in a side-by-side manner. When forespore membrane formation begins at metaphase II, the Mcp4 signals assemble at the lagging face of the dividing nuclei. At this stage, they are sandwiched between F-actin and the nucleus. Mcp4, in turn, appears to sandwich F-actin with Meu14. In mcp4Δ cells at anaphase II, the F-actin, which is normally dumbbell-shaped, adopts an abnormal balloon shape. Spores of mcp4Δ cells were sensitive to NaCl, although their shape and viability were normal. Taken together, we conclude that Mcp4 plays a role in the accurate positioning of F-actin during S. pombe meiosis.

The Current Using Situation of SBS Modified Asphalt in China and a New Method of Monitoring SBS Modifier Dosage

2013 ◽  
Vol 706-708 ◽  
pp. 575-578
Author(s):  
Jia Le Song ◽  
Chang Yong Ye ◽  
Bai Lin Wang ◽  
Zhi Mi Zhou ◽  
Yan Xiao ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Temperature ◽  
Research Group ◽  
Detection Method ◽  
Microscopic Analysis ◽  
Construction Site ◽  
Modified Asphalt ◽  
Sbs Modified Asphalt ◽  
The Relationship ◽  
Fluorescence Microscopic Analysis

The relationship between the SBS modifier types and asphalt components on the effect of modified asphalt properties was discussed in this paper. Higher block ratio, higher molecular weight and star-like structure of SBS can improve modified asphalt high temperature properties. Colloid content decrease leading the processing easier, result stability decline. Our research group invented a chemical titration combined with fluorescence microscopic analysis detection method monitoring SBS modifier dosage. This method applied in highway construction site, the effect is significant.

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