scholarly journals Use of a whole-slide imaging system to assess the presence and alteration of lymphatic vessels in joint sections of arthritic mice

2012 ◽  
Vol 88 (8) ◽  
pp. 428-439 ◽  
Author(s):  
JX Shi ◽  
QQ Liang ◽  
YJ Wang ◽  
RA Mooney ◽  
BF Boyce ◽  
...  
Keyword(s):  
Imaging System ◽  
Lymphatic Vessels ◽  
Whole Slide Imaging

Abstract 13143: Safety Evaluation of Therapeutic Angiogenesis With Adipose-derived Regenerative Cells for Ischemic Limb in a Tumor Bearing Model

Circulation ◽  
2020 ◽  
Vol 142 (Suppl_3) ◽  
Author(s):  
Junya Suzuki ◽  
Yuuki Shimizu ◽  
Kazuhito Tsuzuki ◽  
Zhongyue Pu ◽  
Shukuro Yamaguchi ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Imaging System ◽  
Lymphatic Vessels ◽  
Blood Perfusion ◽  
Capillary Density ◽  
Therapeutic Angiogenesis ◽  
Control Group ◽  
In Vivo Bioluminescence Imaging ◽  
Regenerative Cells

Introduction: Implantation of adipose-derived regenerative cells (ADRC) is a promising novel strategy to augment angiogenesis and blood perfusion recovery in ischemic diseases with no other therapeutic option. However, there is a clinical concern underlying therapeutic angiogenesis that implantation of ADRC may promote tumor growth and metastasis via remote angiogenesis. Accordingly, we tested whether therapeutic angiogenesis with ADRC against hindlimb ischemia (HLI) would affect remote tumor growth and angiogenesis in a tumor-bearing mouse ischemic hindlimb model. Methods and results: B16F10-Luc (murine melanoma cells expressing luciferase, 1x106 cells/animal) were implanted to C57BL/6J mice’s (male, 8-10 weeks old, n=10) back. Mice were subjected to unilateral HLI surgery one day after tumor implantation. Then, mice were randomly assigned to the control group or the ADRC group (n=5 for each). ADRC (1x106 cells/animal) or PBS were implanted/injected into ischemic hindlimb muscles one day after the surgery. Blood perfusion recovery in HLI by laser Doppler perfusion imaging system and tumor size by a caliper were measured every week up to 21 days after surgery. At POD 21, tumor weight and luciferase activity in primary tumors obtained by in vivo bioluminescence imaging system were also evaluated. Immunohistochemistry by CD31 or LYVE1 staining was performed to detect feeder arteries or outflow lymphatic vessels in tumors. The results demonstrated that better blood perfusion recovery and more capillary density in HLI was observed in the ADRC group than in the control group (p<0.05, respectively). However, there were no significant differences in terms of tumor volume (p=0.95), tumor weight (p=0.88) and luciferase activity of primary tumor (p=0.92) between those two groups. No sign of distant metastasis was detected by macroscopic and pathological examination, and by in vivo bioluminescence imaging system in both groups. Further study also revealed that capillary density of peritumoral blood vessels or lymphatic vessels was not augmented by ADRC implantation into remote HLI. Conclusions: Our data indicated that therapeutic angiogenesis with ADRC implantation against HLI did not promote remote tumor growth, angiogenesis and metastasis.

scholarly journals Minimally invasive method for determining the effective lymphatic pumping pressure in rats using near-infrared imaging

2014 ◽  
Vol 306 (5) ◽  
pp. R281-R290 ◽  
Author(s):  
Tyler S. Nelson ◽  
Ryan E. Akin ◽  
Michael J. Weiler ◽  
Timothy Kassis ◽  
Jeffrey A. Kornuta ◽  
...  
Keyword(s):  
Minimally Invasive ◽  
Near Infrared ◽  
Infrared Imaging ◽  
Imaging System ◽  
Lymphatic Vessels ◽  
In Vivo Studies ◽  
No Donor ◽  
Pressure Cuff ◽  
Research Platform

The ability to quantify collecting vessel function in a minimally invasive fashion is crucial to the study of lymphatic physiology and the role of lymphatic pump function in disease progression. Therefore, we developed a highly sensitive, minimally invasive research platform for quantifying the pumping capacity of collecting lymphatic vessels in the rodent tail and forelimb. To achieve this, we have integrated a near-infrared lymphatic imaging system with a feedback-controlled pressure cuff to modulate lymph flow. After occluding lymphatic flow by inflating a pressure cuff on the limb or tail, we gradually deflate the cuff while imaging flow restoration proximal to the cuff. Using prescribed pressure applications and automated image processing of fluorescence intensity levels in the vessels, we were able to noninvasively quantify the effective pumping pressure (Peff, pressure at which flow is restored after occlusion) and vessel emptying rate (rate of fluorescence clearance during flow occlusion) of lymphatics in the rat. To demonstrate the sensitivity of this system to changes in lymphatic function, a nitric oxide (NO) donor cream, glyceryl trinitrate ointment (GTNO), was applied to the tails. GTNO decreased Peff of the vessels by nearly 50% and the average emptying rate by more than 60%. We also demonstrate the suitability of this approach for acquiring measurements on the rat forelimb. Thus, this novel research platform provides the first minimally invasive measurements of Peff and emptying rate in rodents. This experimental platform holds strong potential for future in vivo studies that seek to evaluate changes in lymphatic health and disease.

scholarly journals InstantScope: a low-cost whole slide imaging system with instant focal plane detection

2015 ◽  
Vol 6 (9) ◽  
pp. 3210 ◽  
Author(s):  
Kaikai Guo ◽  
Jun Liao ◽  
Zichao Bian ◽  
Xin Heng ◽  
Guoan Zheng
Keyword(s):  
Focal Plane ◽  
Imaging System ◽  
Low Cost ◽  
Whole Slide Imaging

scholarly journals Comparison of normal hindlimb lymphatic systems in rats with detours present after lymphatic flow blockage

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0260404
Author(s):  
Yuiko Suzuki ◽  
Yukari Nakajima ◽  
Toshio Nakatani ◽  
Mayumi Okuwa ◽  
Junko Sugama
Keyword(s):  
Lymph Node ◽  
Lymphatic Vessel ◽  
Near Infrared ◽  
Imaging System ◽  
Lymphatic Vessels ◽  
Lymphatic Drainage ◽  
Lymphatic Flow ◽  
Fluorescence Imaging System ◽  
Normal Rat ◽  
Flow Blockage

In the present study, we aimed to identify the normal hindlimb lymphatic systems in rats and compare them with the detours after lymphatic flow blockage. The lymphatic systems of the hindlimbs of normal rats were investigated via lymphography using a near-infrared fluorescence imaging system. The lymphatic vessels were stained using Evans Blue. The lymphatic flow was blocked through lymphatic vessel ligation combined with inguinal and popliteal lymph node dissection. Detours that appeared after 30 days were visualized using lymphography and immunostaining with anti-podoplanin antibodies. Three main results were obtained in the present study. First, the deep medial system, the superficial medial system, a connection between the superficial and deep medial lymphatic systems, and the superficial lateral system, were elucidated. Second, three types of detours, namely the detour of the lateral abdomen, the detour to the lymphatic vessel near the midline of the abdomen, and the detour to the contralateral inguinal lymph node, were identified after lymphatic flow blockage. Lastly, detours were located in the fatty layer above the panniculus carnosus muscle and their lumina were wide. The histology suggested that the detour was a pre-collecting lymphatic vessel. Lymphatic routes in the rat hindlimbs after lymphatic flow blockage were different from those of the normal rat lymphatic system. It was suggested that the detour is a pre-collecting lymphatic vessel and that encouraging its development may be a new method of simple lymphatic drainage.

scholarly journals Low-cost whole slide imaging system withsingle-shot autofocusing based on colormultiplexed illumination and deep learning

2021 ◽  
Author(s):  
Kaifa Xin ◽  
Shaowei Jiang ◽  
xu chen ◽  
Yonghong He ◽  
Jian Zhang ◽  
...  
Keyword(s):  
Deep Learning ◽  
Imaging System ◽  
Low Cost ◽  
Whole Slide Imaging

Integrating a Web-Based Whole-Slide Imaging System and Online Questionnaires in a National Cytopathology Peer Comparison Educational Program in Taiwan

2015 ◽  
Vol 59 (3) ◽  
pp. 278-283 ◽  
Author(s):  
Jen-Fan Hang ◽  
Wen-Yih Liang ◽  
Chih-Yi Hsu ◽  
Chiung-Ru Lai
Keyword(s):  
Educational Program ◽  
Large Scale ◽  
Imaging System ◽  
Whole Slide Imaging ◽  
Easy Method ◽  
Web Based ◽  
Glass Slides ◽  
Promising Solution ◽  
First Time ◽  
Whole Slide Images

Objective: In a peer comparison educational program, transferring glass slides between laboratories and collecting responses are time- and cost-consuming. Integrating a web-based whole-slide imaging (WSI) system and online questionnaires may serve as a promising solution. Study Design: Five gynecologic Papanicolaou-stained smears and 5 nongynecologic slides were selected. The 10 whole-slide images were acquired by a Leica SCN-400 system and released via an Aperio eSlide Manager. Online questionnaires generated by Google Forms with access to the 10 whole-slide images were released to all the practitioners in Taiwan by e-mail. After closing the program, an online posttest feedback survey was conducted. Results: A total of 302 participants joined the gynecologic test, and 291 joined the nongynecologic test. The correct interpretation rates were 81.8-93.7% in the former and 28.5-93.1% in the latter. In the posttest feedback survey, there were 63.2% of the participants reporting first-time WSI experience, and 97.9% of them said they would like to participate in a similar program again. Conclusion: Integrating a web-based WSI system and online questionnaires is an easy method to access nationwide practitioners. Participants can make interpretations using WSI even without prior experience. The model is valuable for those who want to initiate a large-scale cytopathology peer comparison educational program.

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