Biological activity and fatty acid composition of Caesar's mushroom

Abstract Purpose Polysorbate excipients are commonly used as surfactants to stabilize therapeutic proteins in formulations. Degradation of polysorbates could lead to particle formation and instability of the drug formulation. We investigated how the fatty acid composition of polysorbate 80 impacts the degradation profile, particle formation, and product stability under stress conditions. Methods Two polysorbate 80-containing therapeutic protein formulations were reformulated with either Polysorbate 80 NF synthesized from a fatty acid mixture that contains mainly oleic acid (≥58%) or a version of polysorbate 80 synthesized with high oleic acid (>98%). Stress conditions, including high temperature and esterase spiking, were applied and changes to both the polysorbate and the therapeutic protein product were investigated for stability, purity, innate immune response and biological activity. Results The addition of esterase and storage at 37°C led to significant hydrolysis of the polysorbate and increases in sub-visible particle formation for both polysorbates tested. The fatty acid composition of polysorbate 80 did not directly alter the stability profile of either therapeutic protein as measured by size exclusion chromatography, or significantly impact innate immune response or biological activity. However, formulations with Polysorbate 80 NF showed greater propensity for sub-visible particle formation under stress conditions. Conclusions These results suggest that composition of fatty acids in polysorbate 80 may be a promoter for sub-visible particulate formation under the stress conditions tested but may not impact protein aggregation or biological activity.

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LIPOPOLYSACCHARIDES OF RALSTONIA SOLANACEARUM: FATTY ACID COMPOSITION AND BIOLOGICAL ACTIVITY

Microbiology&Biotechnology ◽

10.18524/2307-4663.2012.2(18).92887 ◽

2012 ◽

Vol 0 (2(18)) ◽

pp. 20-29

Author(s):

Р. В. Грицай ◽

О. В. Голубець ◽

О. С. Броварська ◽

Л. Д. Варбанець

Keyword(s):

Biological Activity ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Ralstonia Solanacearum

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Fatty Acid Composition and Biological activity of Zizyphus spina-christi L Seeds Oil from Sudan

International Journal of Engineering and Applied Sciences (IJEAS) ◽

10.31873/ijeas.6.11.07 ◽

2019 ◽

Vol 6 (11) ◽

Author(s):

Fath El-Rahman A. ◽

Munged I. ◽

Tegani A. ◽

Ibrahim A. ◽

Mohamed A. ◽

...

Keyword(s):

Biological Activity ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition

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Fatty acid composition and biological activity of four olive oils from Kabylia (Algeria) against Rhyzopertha dominica (Coleoptera: Bostrychidae) infesting wheat seeds

Studia Universitatis Babeş-Bolyai Biologia ◽

10.24193/subbbiol.2021.1.01 ◽

2021 ◽

Vol 66 (1) ◽

pp. 51-72

Author(s):

Safia Kerbel ◽

Ines Abdelli ◽

Hakim Azzi ◽

Jean-Francois Debras ◽

Abdellah Kellouche

Keyword(s):

Biological Activity ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Olive Oil ◽

Acid Composition ◽

Soft Wheat ◽

Rhyzopertha Dominica ◽

Contact Toxicity ◽

Olive Oils ◽

Wheat Seeds

The use of conventional insecticides is one of the most widely used methods of controlling pests of stored grains. But the presence of toxic residues in treated commodities and the emergence of insect resistant strains are becoming a growing concern. Olive oil is well known throughout the world for its benefits to human health, but little known for its biological activity against insect pests.The aim of this work is to study the fatty acid composition and the insecticidal activity of oils according to origin of plantation, against one of the main insect pests of stored grain Rhyzopertha dominica (Coleoptera: Bostrychidae). The olive oils were obtained using an oleodoser from olives of the 'Chemlal' variety harvested in 4 olive groves in Kabylia (Algeria) and the analysis of the fatty acid composition was carried out by gas chromatography. The main fatty acids found are oleic, palmitic and linoleic acids. Biological tests conducted under laboratory conditions, at a temperature of 30±1°C and a relative humidity of 70 ± 5 %, revealed that the 4 olive oils, applied on soft wheat grains, showed a contact toxicity against R. dominica. The toxicity of the oils varied as a function of the dose and the duration of treatments. After 24 h of exposure, all oils tested at the highest dose (0.4 mL/25 g) were found to be highly toxic to adults of R. dominica, with mortality rates ranging from 72.5 to 95 %. The toxicity of the 4 oils based on the LD50 (mL/25 g) values for 24 h mortality is established as follows: Maatkas (213), Bachloul (232), Tadmait (234) and M'Chedellah (263). The number of the F1 offspring decreases as the dose of oil is increased to reach zero with the highest dose, for all treatments. All oils tested completely preserve soft wheat seeds from R. dominica attacks using the same highest dose. On the other hand, results also revealed that treatments with olive oil do not affect the germination capacity of soft wheat seeds.

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Fatty Acid Composition and Biological Activity of Supercritical Extracts from Arctic Brown Algae Fucus vesiculosus

Russian Journal of Physical Chemistry B ◽

10.1134/s1990793117070065 ◽

2017 ◽

Vol 11 (7) ◽

pp. 1144-1152 ◽

Cited By ~ 3

Author(s):

K. G. Bogolitsyn ◽

P. A. Kaplitsin ◽

L. K. Dobrodeeva ◽

A. S. Druzhinina ◽

D. V. Ovchinnikov ◽

...

Keyword(s):

Biological Activity ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Brown Algae ◽

Fucus Vesiculosus

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Effect of chilling and subsequent storage at 20oC on electrolyte leakage and phospholipid fatty acid composition of tomato pericarp

Physiologia Plantarum ◽

10.1034/j.1399-3054.1993.870412.x ◽

1993 ◽

Vol 87 (4) ◽

pp. 522-527 ◽

Cited By ~ 1

Author(s):

M. Bergevin ◽

G. P. L'Heureux ◽

J. E. Thompson ◽

C. Willemot

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Electrolyte Leakage ◽

Phospholipid Fatty Acid ◽

Phospholipid Fatty Acid Composition ◽

Subsequent Storage

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Platelet Aggregation in Finnish Men and Its Relation to Fatty Acids in Platelets, Plasma and Adipose Tissue

Thrombosis and Haemostasis ◽

10.1055/s-0038-1660071 ◽

1985 ◽

Vol 54 (03) ◽

pp. 563-569 ◽

Cited By ~ 19

Author(s):

M K Salo ◽

E Vartiainen ◽

P Puska ◽

T Nikkari

Keyword(s):

Fatty Acids ◽

Adipose Tissue ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Platelet Aggregation ◽

Acid Composition ◽

Saturated Fatty Acids ◽

Free Living ◽

Ω3 Fatty Acids ◽

Induced Aggregation

SummaryPlatelet aggregation and its relation to fatty acid composition of platelets, plasma and adipose tissue was determined in 196 randomly selected, free-living, 40-49-year-old men in two regions of Finland (east and southwest) with a nearly twofold difference in the IHD rate.There were no significant east-southwest differences in platelet aggregation induced with ADP, thrombin or epinephrine. ADP-induced platelet secondary aggregation showed significant negative associations with all C20-C22 ω3-fatty acids in platelets (r = -0.26 - -0.40) and with the platelet 20: 5ω3/20: 4ω 6 and ω3/ ω6 ratios, but significant positive correlations with the contents of 18:2 in adipose tissue (r = 0.20) and plasma triglycerides (TG) (r = 0.29). Epinephrine-induced aggregation correlated negatively with 20: 5ω 3 in plasma cholesteryl esters (CE) (r = -0.23) and TG (r = -0.29), and positively with the total percentage of saturated fatty acids in platelets (r = 0.33), but had no significant correlations with any of the ω6-fatty acids. Thrombin-induced aggregation correlated negatively with the ω3/6ω ratio in adipose tissue (r = -0.25) and the 20: 3ω6/20: 4ω 6 ratio in plasma CE (r = -0.27) and free fatty acids (FFA) (r = -0.23), and positively with adipose tissue 18:2 (r = 0.23) and 20:4ω6 (r = 0.22) in plasma phospholipids (PL).The percentages of prostanoid precursors in platelet lipids, i. e. 20: 3ω 6, 20: 4ω 6 and 20 :5ω 3, correlated best with the same fatty acids in plasma CE (r = 0.32 - 0.77) and PL (r = 0.28 - 0.74). Platelet 20: 5ω 3 had highly significant negative correlations with the percentage of 18:2 in adipose tissue and all plasma lipid fractions (r = -0.35 - -0.44).These results suggest that, among a free-living population, relatively small changes in the fatty acid composition of plasma and platelets may be reflected in significant differences in platelet aggregation, and that an increase in linoleate-rich vegetable fat in the diet may not affect platelet function favourably unless it is accompanied by an adequate supply of ω3 fatty acids.

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Health Benefits of Pumpkin Seed and Nutrition Profile of 35 Pumpkin Accessions

EDIS ◽

10.32473/edis-hs1312-2017 ◽

2017 ◽

Vol 2017 (6) ◽

Author(s):

Geoffrey M. Meru ◽

Yuqing Fu ◽

Dayana Leyva ◽

Paul Sarnoski ◽

Yavuz Yagiz

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Health Benefits ◽

Pumpkin Seed ◽

Production Practices

This article aims to summise production and nutrition aspects of pumpkin seed. Specifically, it focuses on health benefits of the seeds, production practices and provides data on the oil, protein and fatty acid composition of 35 pumpkin accessions.

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