Evaluation of phenolic contents, free radical scavenging activity and functional group analysis of the leaf extract of a medicinal plant in Niger Delta region

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Free Radical ◽  
Niger Delta ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Delta Region ◽  
Niger Delta Region

Funtumia africana leaves have been used by the people of Niger Delta region of Nigeria to treat diabetes. This study aims to evaluate the polyphenolic contents, antioxidant, and free radical scavenging activities of the leaf crude extract and fractions. The crude aqueous methanolic leaf extract of F. africana was extracted by liquid-liquid partitioning into n-hexane, dichloromethane, ethyl acetate and n-butanol fractions. The free radical scavenging activity of the fractions was measured in vitro by using the ferric-reducing antioxidant power (FRAP), 2, 2-diphenyl-1-picryl dihydrazyl (DPPH), and nitric oxide (NO) assays. Phytochemical screening showed the presence of tannins, saponins, alkaloids, terpenoids, steroids, reducing sugars, cardiac glycosides and flavonoids in the extract and polar fractions. The highest total phenolics, total flavonoids and total antioxidant capacity were found to be 1331.18±41.56 mg GAE/g, 833.96±19.14 mg QUE/g and 83.74±1.88 mgAAE/g respectively in the butanol fraction. The n-hexane and butanol fractions demonstrated strong DPPH scavenging activity with IC50 values of 0.06±0.57 and 0.06±0.5 μg/ml, respectively. The DCM fraction showed the highest NO scavenging activity with IC50 value of 0.741±0.07 mg/ml, while hexane fraction has negligible effect. The butanolic fraction has the highest ferric reducing antioxidant activity with value of 48.46±0.15 mg AAE/g of sample.

scholarly journals Phytochemical Screening and Free Radical Scavenging Activity of Hydroethanolic Leaf Extract of Senna sieberiana DC (Caesalpiniaceae) and its Fractions

2019 ◽  
pp. 1-6
Author(s):  
Alioune Dior Fall ◽  
Awa Ndiaye Sy ◽  
Serigne Ibra Mbacké Dieng ◽  
Abdou Sarr ◽  
Mbaye Dieng
Keyword(s):  
Free Radical ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
Ic50 Values

Objectives: The aim of this study was to identify the phytochemical groups of hydroethanolic leaf extract of Senna sieberiana DC and its fractions and to investigate their free radical scavenging activity. Methods: S. sieberiana leaves were extracted with hydroethanolic solvent. From the hydroethanolic extract 3 fractions were obtained after a liquid/liquid fractionation (dichloromethane, ethyl acetate and water). Phytochemical screening of the leaf extract and its fractions was done using standard reactions. Free radical scavenging activity was assessed using DPPH and ABTS assays.  Results: Tannins, flavonoids, anthracenic derivatives, sterols and triterpenoids were the main phytochemical constituents of the leaf extract and fractions. The hydro-ethanolic leaf extract of Senna sieberiana, its dichloromethane, ethyl acetate and aqueous fractions and ascorbic acid had respective IC50 values of 191.6±3.82 - 495.73±8.96 - 165.8±4.85 - 50.40±2.65 - 19.53±0.13 µg/ml in ABTS assay.  In DPPH assay,  the IC50 values were  44.8 ± 1.22 - 218.93±9.01 - 32.13 ± 1.8 - 26.4±0.11- 4.66±0.07 µg/ml respectively for the leaf extract, dichloromethane, ethyl acetate and aqueous fractions and ascorbic acid. Conclusion: The hydroethanolic leaf extract of S. sieberiana had shown free radical scavenging activity. The aqueous fraction was more active among plant tested samples. 

scholarly journals Radical Scavenging Activity of Edible Fungal Sporocarp Extracts from the Niger Delta Region of Nigeria

2017 ◽  
Vol 9 (4) ◽  
Author(s):  
Ita B. N. ◽  
Eduok S. I.
Keyword(s):  
Free Radical ◽  
Niger Delta ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Aqueous Extracts ◽  
Metal Chelating ◽  
Niger Delta Region

The radical scavenging activity of acetone, ethanol and aqueous extracts from Agaricus placomyces, Cantharellus cibarius and Pleurotus ostreatus from the Niger Delta region of Nigeria was determined with their metal chelating and reducing power. The extracts exhibited significant free radical scavenging activities in the DPPH assay (IC50 = 2.11 to 4.37 mg/mL) with the aqueous extract from P. ostreatus being the most potent. Also, the aqueous extracts exhibited better metal chelating abilities and reducing power than the ethanol and acetone extracts. In addition, higher content of total phenolics and flavonoids was found in the aqueous extract of A. placomyces (55.63mg GAE/g) and P. ostreatus (15.55 mgQE/g) respectively, whereas ascorbic acid ranged from 0.33 to 1.22 mgAAE/ in all the extracts. Overall, our results indicate that mushrooms particularly Agaricus placomyces, and Pleurotus ostreatus contain high amount of polyphenolic compounds with significant free radical scavenging abilities, notable metal chelating activities and promising reducing power. These properties indicate that the mushrooms are functional foods with the potential to attenuate oxidative stress associated with free radical damage in biological systems.

Evaluation of In-vitro Antioxidant Potential and Screening, Characterization of Phytochemicals Using hydroethanolic leaf Extract of Caesalpinia Pulcherrima

2020 ◽  
Vol 09 ◽  
Author(s):  
Akila Ilangovan ◽  
Raghu V ◽  
Venugopal R ◽  
Vijay N ◽  
Yellasamy Y ◽  
...  
Keyword(s):  
Free Radical ◽  
Functional Groups ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Phytochemical Analysis ◽  
Scavenging Activity

Introduction: Novel Therapeutic Phytochemical studies are based on the use of plants in the production of drugs. This present study was carried out to the screening of phytochemicals and quantitative analysis, to assess In-vitro Antioxidant activities of the hydroethanolic leaf extract of Caesalpinia pulcherrima. FT-IR and GC-MS analysis of hydroethanolic leaf extract of Caesalpinia pulcherrima. Methods: The investigation of qualitative and determination of quantitative phytochemical analysis were performed using standard procedures. The total alkaloid, flavonoid, phenols, and tannin content were determined spectrophotometrically. DPPH (2,2-Diphenyl-1-picrylhydrazyl) free radical scavenging activity, ABTS (2,2’-azino-bis (3-ethylbenzothiazoline-6- sulfonic acid) free radical scavenging activity of hydroethanolic leaf extract of Caesalpinia pulcherrima was estimated by standard protocol. The identification of functional groups using plant leaf extracts by FTIR and GCMS. Results: The hydroethanolic leaf extract of Caesalpinia pulcherrima gives positive results for alkaloids, flavonoids, phenols, carbohydrates, tannins, amino acids, and proteins. The content of total alkaloids, flavonoids, phenols, and tannins varies from 8.4 AE/g, 3.3 CE/g, 2.9, and 3.2 GAE/g respectively. The antioxidant assay and IC50 values were found to be DPPH (43.3µg/ml), ABTS (46.1µg/ml), and ascorbic acid (41µg/ml), respectively and concentration ranging from 20 to 100µgml. Functional groups were also identified using the extract by FTIR and GCMS. Conclusions: From this work, it can be concluded that the presence of Phyto-component makes the plant useful in the treatment of various diseases. As such, it has been found that the hydroethanolic leaf extract of the plant contains more components and is beneficial for further research.

scholarly journals Phytochemical Screening and Free Radical Scavenging Activity of Cinnamomum tamala Leaf Extract

2021 ◽  
Vol 7 (2) ◽  
Author(s):  
Rani Raksha ◽  
Kumar Rajesh ◽  
Sharma Preeti ◽  
Hajam Younis Ahmad ◽  
Rai Seema
Keyword(s):  
Free Radical ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
Bioactive Components ◽  
Cinnamomum Tamala

Traditional plant-based medicines are still needed by the whole world for their primary healthcare benefits. The phytochemicals or plant extract could be used to treat different diseases and new formulation for the drug discovery in pharmaceuticals. Cinnamomum tamala is commonly called as Indian bay leaf or Tejpatta. The leaves and bark of Cinnamomum tamala are used to cure various diseases due to its various properties including astringent, stimulant and carminative. Hence, the objective of this study was to determine the comparative phytochemical screening and free radical scavenging activity of the leaf of Cinnamomum tamala. To achieve this, extract was prepared in three solvents (ethanol, ethanol, aqueous and chloroform). Phytochemical screening (qualitative and quantitative) was evaluated in all the three fractions to compare the solubility of various bioactive components. Phytochemical screening showed the presence of polyphenols, flavonoids, alkaloids, flavones and flavonols, tannins, carbohydrates, amino acids and proteins, saponins and glycosides in leaves of Cinnamomum tamala. Quantitative analysis showed that the total polyphenolic content, total flavonoids content, total alkaloids and total flavones and flavonols content in the hydroalcoholic extract was 48.1 mg GA (gallic acid)/g, 22.1 mg QE (Quercetin)/g, 59.9 mg/g and 1.75 mg RE (Rutin)/g, respectively. DPPH free radical assay revealed that Cinnamomum tamala hydroalcoholic leaf extract at a 100 µm/ml concentration showed 96.99± 0.99% inhibition activity. It can be concluded that most of the bioactive components are found soluble in hydroalcoholic solvent. Cinnamomum tamala hydroalcoholic leaf extract contains various bioactive and also exhibits significant free radical scavenging activity. Hence, it can be used as an alternative remedy for the treatment of various diseases.

Free radical scavenging activity and immunomodulatory effect of Stachytarpheta angustifolia leaf extract

2010 ◽  
Vol 119 (4) ◽  
pp. 1409-1416 ◽  
Author(s):  
Francis M. Awah ◽  
Peter N. Uzoegwu ◽  
Julius O. Oyugi ◽  
John Rutherford ◽  
Patrick Ifeonu ◽  
...  
Keyword(s):  
Free Radical ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Immunomodulatory Effect ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity
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