SCREENING OF RHIZOBACTERIA FROM ONION RHIZOSPHERE CAN INDUCE SYSTEMIC RESISTANCE TO BACTERIAL LEAF BLIGHT DISEASE ON ONION PLANTS

In modern cultivation processes indiscriminate use of pesticides and fertilizers, has led to substantialpollution of soil, air and water. So, there is an urgent need to solve the problem. Rhizobacteria are bacteria thatcolonize plant roots, and these bacteria are known to stimulate growth and thereby reduce incidence of plantdisease by direct and indirect mechanisms. A total of 136 rhizobacteria isolates were isolated from differentrhizosphere soils in central areas of production of onions in Indonesia. These isolates were screened for theircapability to enhance growth and protect onions against bacterial leaf blight disease-caused by Xanthomonasaxonopodis pv.allii. The results showed that ten isolates can enhance growth and protect onions against bacterialleaf blight diseases. Five isolates were isolated from West Sumatra, four isolates from Java and one isolated fromNorth Sumatra. All isolates produced indol-3-acetic acid with different concentrations. Molecular identificationof ten isolates belong to Bacillus sp, Pseudomonas sp, Stenotrophomonas sp and Serratia sp.

Download Full-text

SCREENING OF RHIZOBACTERIA FROM ONION RHIZOSPHERE CAN INDUCE SYSTEMIC RESISTANCE TO BACTERIAL LEAF BLIGHT DISEASE ON ONION PLANTS

International Journal of Agricultural Sciences ◽

10.25077/ijasc.1.1.81-89.2015 ◽

2015 ◽

Vol 1 (1) ◽

pp. 81

Author(s):

Milda Ernita, Trimurti Habazar, Nasrun Jamsari

Keyword(s):

Leaf Blight ◽

Plant Roots ◽

Bacterial Leaf Blight ◽

Systemic Resistance ◽

Pseudomonas Sp ◽

Induce Systemic Resistance ◽

Bacillus Sp ◽

West Sumatra ◽

Blight Disease ◽

North Sumatra

In modern cultivation processes indiscriminate use of pesticides and fertilizers, has led to substantialpollution of soil, air and water. So, there is an urgent need to solve the problem. Rhizobacteria are bacteria thatcolonize plant roots, and these bacteria are known to stimulate growth and thereby reduce incidence of plantdisease by direct and indirect mechanisms. A total of 136 rhizobacteria isolates were isolated from differentrhizosphere soils in central areas of production of onions in Indonesia. These isolates were screened for theircapability to enhance growth and protect onions against bacterial leaf blight disease-caused by Xanthomonasaxonopodis pv.allii. The results showed that ten isolates can enhance growth and protect onions against bacterialleaf blight diseases. Five isolates were isolated from West Sumatra, four isolates from Java and one isolated from North Sumatra. All isolates produced indol-3-acetic acid with different concentrations. Molecular identification of ten isolates belong to Bacillus sp, Pseudomonas sp, Stenotrophomonas sp and Serratia sp.

Download Full-text

THE POTENCY OF Bacillus sp. AND Pseudomonas sp. AS BIOLOGICALCONTROL AGENTS AGAINST CORN LEAF BLIGHT DISEASE CAUSED BY Pantoea sp.

AGRIVITA Journal of Agricultural Science ◽

10.17503/agrivita-2013-35-2-p103-109 ◽

2013 ◽

Vol 35 (2) ◽

Author(s):

Cokorda Javandira ◽

Luqman Qurata Aini ◽

Ariffin Noor Sugiharto ◽

Abdul Latief Abadi

Keyword(s):

Leaf Blight ◽

Pseudomonas Sp ◽

Bacillus Sp ◽

Blight Disease ◽

Corn Leaf Blight

Download Full-text

SKRINING DAN IDENTIFIKASI ISOLAT BAKTERI ENDOFIT UNTUK MENGENDALIKAN PENYAKIT HAWAR DAUN BAKTERI PADA BAWANG MERAH

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ◽

10.23960/j.hptt.213167-178 ◽

2013 ◽

Vol 13 (2) ◽

pp. 167-178

Author(s):

Zurai Resti ◽

Trimurti Habazar ◽

Deddi Prima Putra ◽

Nasrun Nasrun

Keyword(s):

Bacillus Cereus ◽

Endophytic Bacteria ◽

Disease Incidence ◽

Leaf Blight ◽

Bacterial Leaf Blight ◽

Bacillus Sp ◽

In Planta ◽

16S Rrna Sequence ◽

Randomized Design ◽

Blight Disease

Screening and identification of endophytic bacteria to control bacterial leaf blight disease on Shallot. The experiment was conducted in Laboratory and Green House, from January to June 2012. Laboratory experiment consisted of three steps: (1) isolation of endophytic bacteria from healthy onion roots, (2)In planta /screening of endophytic isolates capable of reducing bacterial leaf blight disease, and (3) molecular identification of potential endophytic isolates. Treatments of in planta test were arranged in Completely Randomized Design. Collected isolates were tested for their capability in controlling bacterial leaf blight disease on shallot. The variables observed were disease incidence, disease severity, and shallot yield. The results showed that out of 82 isolates successfully isolated, 56 isolates (68.29%) were Gram positive, and 26 isolate (31.71%) were Gram negative. All isolates were HR negative and pathogenicity negative. Six endophytic isolates showed better performance in inducing resistance and increasing onion yield. Based on 16S rRNA sequence the six isolates were Bacillus cereus strain P14 , Bacillus cereus strain Se07, Bacillus sp H1, Bacillus sp SJ1 and Serratia marcescens strain PPM4.

Download Full-text

CHARACTERIZATION OF ENDOPHYTIC BACILLUS ISOLATED FROM SHALLOT ROOT AS BIOCONTROL OF BACTERIAL LEAF BLIGHT DISEASE

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ◽

10.23960/j.hptt.11831-38 ◽

2018 ◽

Vol 18 (1) ◽

pp. 31

Author(s):

Zurai Resti ◽

Trimurti Habazar ◽

Deddi Prima Putra ◽

Nasrun .

Keyword(s):

Salicylic Acid ◽

Endophytic Bacteria ◽

Root Colonization ◽

Linear Regression Analysis ◽

Leaf Blight ◽

Bacterial Leaf Blight ◽

Bacillus Sp ◽

In Planta ◽

Blight Disease

Characterization of Endophytic Bacillus Isolated from Shallot Root as Biocontrol of Bacterial Leaf Blight Disease. Endophytic Bacillus isolated from the roots of healthy shallots has potential as biological control agent of bacterial leaf blight (BLB) disease. Based on the in planta screening, four endophytic bacteria that capable of controlling BLB diseases from the group of Bacillus were obtained, such as Bacillus cereus P14, Bacillus cereus Se07, Bacillus sp. HI, and Bacillus sp. SJI. The aims of this study were to investigate the characteristics of endophytic Bacillus that capable of controlling BLB disease and to determine the characteristic that most responsible in the disease control. This study method was descriptive. The characteristics observed were production of antibiotics by paper disc method, production of salicylic acid by capillary electrophoresis, and root colonization by in planta method. Linear regression analysis was used to determine the characteristic most responsible in the disease control. The results showed that four endophytic Bacillus were capable of producing salicylic acid and colonizing the root, and three of them were also capable of producing antibiotic. Salicylic acid production varied ranging from 13.96 to 14.72 ppm mL-1. Three endophytic Bacillus were capable of producing antibiotic with inhibition zone of 16.25 to 20.25 mm. Endophytic Bacillus was able to colonize the shallot roots with a population of bacteria ranged from 3.20 × 105-6, 20 × 105 CFU g-1 root. Based on the correlation coefficient of linear regression analysis, the root colonization of endophytic bacteria played the role in decreasing BLB disease.

Download Full-text